ASTM D5120-90(2004)
(Test Method)Standard Test Method for Inhibition of Respiration in Microbial Cultures in the Activated Sludge Process
Standard Test Method for Inhibition of Respiration in Microbial Cultures in the Activated Sludge Process
SIGNIFICANCE AND USE
The objectives of the respiration inhibition tests may be defined by the interests of the user, but the test method is designed primarily for examination of the inhibition response with operating microbial systems such as an activated sludge process treating domestic or industrial wastes.
Different apparatus exist that facilitate continuous or continual measurement of respiration in microbial systems and each may be used as the tool to observe respiration in this test method.
Respirometry may utilize any apparatus and technique that will achieve the determination of respiration rate. A number of devices are presented in Appendix X1. Equivalency in the experimental capability of each device is not implied. The analyst should select the respirometric approach that best suits his needs.
The inhibitory effect of a test candidate is identified more completely by examining inhibition over a range of concentrations, such as determining the EC50. The use of aerated containers permits concurrent management of a series of cell suspensions. A respirometer for each cell suspension might also be used.
SCOPE
1.1 This test method covers a batch procedure that evaluates the impact of selected wastewaters, materials, or specific compounds on the respiration rate of an aqueous microbial culture, such as activated sludge.
1.2 Alternative procedures for measurement of microbial activity, such as adenosine 5` triphosphate (ATP), specific substrate utilization, etc. are not within the scope of this test method.
1.3 The results obtained are based on comparisons in a specific test series that examines a range of concentrations of the potentially inhibitory test candidate using batch methods in a laboratory. Results are completed in a short time frame (a few hours).
1.4 The test results are specific to the microbial culture used. Microbial culture from different wastewater treatment plants will differ in kinds and numbers of organisms, and performance capability. Thus, there is no basis for comparing results for microbial cultures from different treatment facilities.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:D5120–90(Reapproved2004)
Standard Test Method for
Inhibition of Respiration in Microbial Cultures in the
1
Activated Sludge Process
This standard is issued under the fixed designation D5120; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 EC —the concentration of the test candidate in this
50
procedure (volume percent or mg/L) that results in a reduction
1.1 Thistestmethodcoversabatchprocedurethatevaluates
of respiration rate to 50 % of that observed for the control.
the impact of selected wastewaters, materials, or specific
compounds on the respiration rate of an aqueous microbial
4. Summary of Test Method
culture, such as activated sludge.
4.1 This test method utilizes respiration rate as the indicator
1.2 Alternative procedures for measurement of microbial
of microbial activity.
activity, such as adenosine 58 triphosphate (ATP), specific
4.2 A batch system that contains a microbial culture (re-
substrate utilization, etc. are not within the scope of this test
turned activated sludge from the process or a culture main-
method.
tained in the laboratory), selected nutrient dose, and a dilution
1.3 The results obtained are based on comparisons in a
of a compound, substance, wastewater, etc. (test candidate) is
specific test series that examines a range of concentrations of
prepared in a container in the laboratory. The batch system is
the potentially inhibitory test candidate using batch methods in
called a “cell suspension.”
alaboratory.Resultsarecompletedinashorttimeframe(afew
4.3 The nutrient dose introduces a large excess of biode-
hours).
gradable substrate thereby putting the culture at a high meta-
1.4 Thetestresultsarespecifictothemicrobialcultureused.
bolic rate. Inhibition of respiration by the test candidate is
Microbial culture from different wastewater treatment plants
observed under these conditions.
willdifferinkindsandnumbersoforganisms,andperformance
4.4 The prepared cell suspension is aerated for a 2-h period.
capability. Thus, there is no basis for comparing results for
At the end of the period, the respiration rate is determined
microbial cultures from different treatment facilities.
using a respirometric or an oxygen uptake technique.
1.5 This standard does not purport to address all of the
4.5 A lower respiration rate for a cell suspension that has
safety concerns, if any, associated with its use. It is the
received the test candidate compared to the respiration rate of
responsibility of the user of this standard to establish appro-
a control cell suspension indicates inhibition of respiration.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
5. Significance and Use
2. Referenced Documents 5.1 The objectives of the respiration inhibition tests may be
2 defined by the interests of the user, but the test method is
2.1 ASTM Standards:
designed primarily for examination of the inhibition response
D4478 Test Method for Oxygen Uptake
with operating microbial systems such as an activated sludge
3. Terminology process treating domestic or industrial wastes.
5.2 Different apparatus exist that facilitate continuous or
3.1 Definitions:
continual measurement of respiration in microbial systems and
3.1.1 respiration rate—the quantitative consumption of
each may be used as the tool to observe respiration in this test
oxygen by an aqueous microbial system. The consumption is
method.
generally expressed as mg O /L/h.
2
5.3 Respirometry may utilize any apparatus and technique
that will achieve the determination of respiration rate. A
1
This Test Method is under the jurisdiction ofASTM Committee D34 on Waste
number of devices are presented inAppendix X1. Equivalency
Management and is the direct responsibility of Subcommittee D34.03 onTreatment,
in the experimental capability of each device is not implied.
Recovery and Reuse.
Current edition approved Feb. 1, 2004. Published February 2004. Originally The analyst should select the respirometric approach that best
approved in 1990. Last previous edition approved in 1995 as D5120–90(1995).
suits his needs.
DOI: 10.1520/D5120-90R04.
5.4 The inhibitory effect of a test candidate is identified
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
more completely by examining inhibition over a range of
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Standards volume information, refer to the standard’s Document Summary page on
concentrations, such as determining the EC . The use of
50
the ASTM website.
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