EN ISO 8192:2007
(Main)Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation (ISO 8192:2007)
Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation (ISO 8192:2007)
ISO 8192:2007 specifies a method for assessing the inhibitory effect of a test material on the oxygen consumption of activated sludge microorganisms.
This method is intended to represent the conditions in biological waste-water treatment plants. It gives information on inhibitory or stimulatory effects after a short exposure (usually 30 min up to 180 min or even more ) of the test material on activated sludge microorganisms.
This method is applicable for testing waters, waste waters, pure chemicals and mixtures of chemicals. Concerning the chemicals, the method refers to those which are soluble under the test conditions. Special care is necessary with materials of low water solubility or high volatility, and with materials abiotically consuming or producing oxygen.
Wasserbeschaffenheit - Bestimmung der Hemmung des Sauerstoffverbrauchs von Belebtschlamm nach Kohlenstoff- und Ammonium-Oxidation (ISO 8192:2007)
Diese Internationale Norm legt ein Verfahren zur Abschätzung der Hemmwirkung eines Testguts auf den Sauerstoffverbrauch von Belebtschlamm-Mikroorganismen fest.
Das Verfahren gibt die Bedingungen in einer biologischen Kläranlage wieder. Es liefert Informationen über hemmende und stimulierende Wirkungen des Testguts nach kurzer Einwirkungszeit (üblicherweise 30 min bis 180 min) auf die Belebtschlamm-Mikroorganismen.
Das Verfahren ist anwendbar auf Wasser, Abwasser, reine Chemikalien und Chemikalienmischungen. Besondere Aufmerksamkeit ist bei Chemikalien mit geringer Wasserlöslichkeit, hoher Flüchtigkeit und Materialien, die abiotisch Sauerstoff verbrauchen oder erzeugen, geboten.
Qualité de l'eau - Essai d'inhibition de la consommation d'oxygène par des boues activées pour l'oxydation du carbone et de l'ammonium (ISO 8192:2007)
L'ISO 8192:2007 spécifie une méthode d'évaluation de l'effet inhibiteur d'un échantillon d'essai sur la consommation d'oxygène des micro-organismes des boues activées.
La présente méthode est conçue pour représenter les conditions dans les stations de traitement des eaux résiduaires biologiques. Elle fournit des informations sur les effets inhibiteurs ou stimulateurs après un temps d'exposition court (habituellement de 30 min à 180 min ou même plus) des micro-organismes des boues activées à l'échantillon d'essai.
Cette méthode est applicable aux essais des eaux, des eaux résiduaires, des substances chimiques pures et des mélanges de substances chimiques. S'agissant des substances chimiques, la méthode porte sur les substances chimiques solubles dans les conditions de l'essai. Des précautions particulières sont nécessaires avec les produits à faible solubilité aqueuse, à haute volatilité et avec ceux qui consomment ou produisent de l'oxygène de manière abiotique.
Kakovost vode - Preskus inhibicije porabe kisika z aktivnim blatom za oksidacijo ogljika in amonija (ISO 8192:2007)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2007
1DGRPHãþD
SIST EN ISO 8192:1997
Kakovost vode - Preskus inhibicije porabe kisika z aktivnim blatom za oksidacijo
ogljika in amonija (ISO 8192:2007)
Water quality - Test for inhibition of oxygen consumption by activated sludge for
carbonaceous and ammonium oxidation (ISO 8192:2007)
Wasserbeschaffenheit - Bestimmung der Hemmung des Sauerstoffverbrauchs von
Belebtschlamm nach Kohlenstoff- und Ammonium-Oxidation (ISO 8192:2007)
Qualité de l'eau - Essai d'inhibition de la consommation d'oxygene par des boues
activées pour l'oxydation du carbone et de l'ammonium (ISO 8192:2007)
Ta slovenski standard je istoveten z: EN ISO 8192:2007
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 8192
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2007
ICS 13.060.70 Supersedes EN ISO 8192:1995
English Version
Water quality - Test for inhibition of oxygen consumption by
activated sludge for carbonaceous and ammonium oxidation
(ISO 8192:2007)
Qualité de l'eau - Essai d'inhibition de la consommation Wasserbeschaffenheit - Bestimmung der Hemmung des
d'oxygène par des boues activées pour l'oxydation du Sauerstoffverbrauchs von Belebtschlamm nach
carbone et de l'ammonium (ISO 8192:2007) Kohlenstoff- und Ammonium-Oxidation (ISO 8192:2007)
This European Standard was approved by CEN on 29 December 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8192:2007: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 8192:2007) has been prepared by Technical Committee ISO/TC 147
"Water Quality" in collaboration with Technical Committee CEN/TC 230 "Water Analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by August 2007, and conflicting national
standards shall be withdrawn at the latest by August 2007.
This document supersedes EN ISO 8192:1995.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.
Endorsement notice
The text of ISO 8192:2007 has been approved by CEN as EN ISO 8192:2007 without any
modifications.
INTERNATIONAL ISO
STANDARD 8192
Second edition
2007-02-01
Water quality — Test for inhibition of
oxygen consumption by activated sludge
for carbonaceous and ammonium
oxidation
Qualité de l'eau — Essai d'inhibition de la consommation d'oxygène par
des boues activées pour l'oxydation du carbone et de l'ammonium
Reference number
ISO 8192:2007(E)
©
ISO 2007
ISO 8192:2007(E)
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ii © ISO 2007 – All rights reserved
ISO 8192:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Principle. 2
5 Reagents, media and inoculum. 3
6 Apparatus . 4
7 Test environment. 5
8 Procedure . 5
9 Calculation and expression of results. 8
10 Validity of the results. 11
11 Test report . 13
Annex A (informative) Examples of measuring units . 14
Annex B (informative) Apparatus for culturing nitrifying activated sludge . 16
Annex C (informative) Overview of the test procedure . 18
Annex D (informative) Mixtures for the preliminary test . 19
Annex E (informative) Example of an inhibition curve . 20
Bibliography . 21
ISO 8192:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 8192 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological
methods.
This second edition cancels and replaces the first edition (ISO 8192:1986), which has been technically revised.
iv © ISO 2007 – All rights reserved
ISO 8192:2007(E)
Introduction
Information generated by this method for assessing the potential toxicity of substances, mixtures and waste
waters to activated sludge may be helpful in estimating the effect of a test material on mixed bacterial
communities in the aquatic environment, especially in aerobic biological treatment systems. The susceptibility
of oxygen uptake by different sub-populations of the bacterial communities to inhibition by chemicals and
waste waters is not necessarily uniform and selective effects may profoundly influence the outcome of the test.
There are two principal groups of microorganisms contributing to the total oxygen consumption by activated
sludge: heterotrophic organisms mainly responsible for the breakdown of carbon-based substrates
(carbonaceous oxidation) and autotrophic nitrifying organisms causing the oxidation of ammonium to nitrate
(nitrification).
This International Standard may be used to assess the toxicity of substances on total oxygen uptake (i.e.
carbonaceous oxidation and nitrification combined) or, by deliberately adding a specific inhibitor of nitrification,
also to assess toxicity of substances to the carbonaceous and nitrification components separately.
For the determination of the nitrification inhibition with this method, a sufficiently nitrifying activated sludge is
[4]
required. Indications of nitrification may be investigated further by application of ISO 9509 .
The user of this method should be aware that particular problems could require the specification of additional
marginal conditions.
The inhibitory effect of a test material may be exerted on both components or it may be exerted predominantly
on only one of them. Nitrification is the process more commonly prone to selective inhibition.
INTERNATIONAL STANDARD ISO 8192:2007(E)
Water quality — Test for inhibition of oxygen consumption by
activated sludge for carbonaceous and ammonium oxidation
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this International Standard
be carried out by suitably trained staff.
1 Scope
This International Standard specifies a method for assessing the inhibitory effect of a test material on the
oxygen consumption of activated sludge microorganisms.
This method is intended to represent the conditions in biological waste-water treatment plants. It gives
information on inhibitory or stimulatory effects after a short exposure (usually 30 min up to 180 min or even
more) of the test material on activated sludge microorganisms.
This method is applicable for testing waters, waste waters, pure chemicals and mixtures of chemicals.
Concerning the chemicals, the method refers to those which are soluble under the test conditions. Special
care is necessary with materials of low water solubility, high volatility and with materials abiotically consuming
or producing oxygen.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
activated sludge
accumulated biological mass (floc) produced in the treatment of waste water by the growth of bacteria and
other microorganisms in the presence of oxygen
[3]
(ISO 6107-1:2004 , definition 2)
3.2
concentration of suspended solids of an activated sludge
amount of solids obtained by filtration or centrifugation of a known volume of activated sludge and dryin
...
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