EN ISO 21427-2:2009

Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 2: Mixed population method using the cell line V79 (ISO 21427-2:2006)

ISO 21427-2:2006 specifies a method for the determination of genotoxicity of water and waste water using a mammalian in vitro test which detects damage, induced by water-soluble substances, to the chromosomes or the mitotic apparatus of V79 cells from the Chinese hamster.
The micronucleus test allows the identification of substances that cause cytogenetic damage which results in the formation of micronuclei containing lagging chromosome fragments and/or whole chromosomes.
The assay is based on the increase in the frequency of micronucleated cells after incubation with and without metabolic activation.

Wasserbeschaffenheit - Bestimmung der Gentoxizität mit dem In-vitro-Mikrokerntest - Teil 2: Verwendung einer nicht-synchronisierten V79-Zellkulturlinie (ISO 21427-2:2006)

Dieser Teil von ISO 21427 legt ein Verfahren zur Bestimmung der Gentoxizität von Wasser und Abwasser mit
einem In-vitro-Test mit Säugetierzellen fest. Mit dem Verfahren können durch Wasserinhaltsstoffe verursachte
zytogenetische Schäden an den Chromosomen oder am Mitoseapparat der V79-Zellen des Chinesischen
Hamsters nachgewiesen werden.
Die dabei entstehenden Mikrokerne können Chromosomenbruchstücke und/oder ganze Chromosomen
beinhalten.
Der Test basiert auf einer Zunahme der Häufigkeit von mikrokernhaltigen Zellen infolge der Einwirkung von
erbgutverändernden Substanzen mit und ohne Zugabe eines metabolisierenden Systems.

Qualité de l'eau - Évaluation de la génotoxicité par le mesurage de l'induction de micronoyaux - Partie 2: Méthode de la population mélangée à l'aide de la lignée de cellules V79 (ISO 21427-2:2006)

L'ISO 21427‑2:2006 spécifie une méthode permettant de déterminer la génotoxicité de l'eau et des eaux usées par un essai réalisé in vitro sur des mammifères pour détecter les dommages induits par les substances hydrosolubles sur les chromosomes ou l'appareil mitotique des cellules V79 du hamster chinois.
L'essai du micronoyau permet d'identifier les substances provoquant des dommages cytogénétiques à l'origine de la formation de micronoyaux contenant des fragments de chromosome retardataires et/ou des chromosomes entiers retardataires.
L'analyse est basée sur l'augmentation de la fréquence des cellules micronucléées après une incubation avec et sans activation métabolique.

Kakovost vode - Vrednotenje genotoksičnosti z merjenjem indukcije mikronukleusov - 2. del: Metoda z nesinhronizirano populacijo celic V79 celične linije (ISO 21427-2:2006)

General Information

• Status: Published
• Publication Date: 24-Mar-2009
• Withdrawal Date: 29-Sep-2009
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 25-Mar-2009
• Due Date: 19-Aug-2009
• Completion Date: 25-Mar-2009
• Ref Project: SIST EN ISO 21427-2:2009 - Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 2: Mixed population method using the cell line V79 (ISO 21427-2:2006)

Relations

• Corrected By: EN ISO 21427-2:2009/AC:2009 - Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei - Part 2: Mixed population method using the cell line V79 (ISO 21427-2:2006/Cor 1:2009)
• Effective Date: 30-May-2009

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 21427-2:2009
01-maj-2009
.DNRYRVWYRGH9UHGQRWHQMHJHQRWRNVLþQRVWL]PHUMHQMHPLQGXNFLMH
PLNURQXNOHXVRYGHO0HWRGD]QHVLQKURQL]LUDQRSRSXODFLMRFHOLF9FHOLþQH
OLQLMH,62
Water quality - Evaluation of genotoxicity by measurement of the induction of micronuclei
- Part 2: Mixed population method using the cell line V79 (ISO 21427-2:2006)
Wasserbeschaffenheit - Bestimmung der Gentoxizität mit dem In-vitro-Mikrokerntest -
Teil 2: Verwendung einer nicht-synchronisierten V79-Zellkulturlinie (ISO 21427-2:2006)
Qualité de l'eau - Évaluation de la génotoxicité par le mesurage de l'induction de
micronoyaux - Partie 2: Méthode de la population mélangée à l'aide de la lignée de
cellules V79 (ISO 21427-2:2006)
Ta slovenski standard je istoveten z: EN ISO 21427-2:2009
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN ISO 21427-2:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 21427-2:2009

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SIST EN ISO 21427-2:2009
EUROPEAN STANDARD
EN ISO 21427-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2009
ICS 13.060.70
English Version
Water quality - Evaluation of genotoxicity by measurement of the
induction of micronuclei - Part 2: Mixed population method using
the cell line V79 (ISO 21427-2:2006)
Qualité de l'eau - Évaluation de la génotoxicité par le Wasserbeschaffenheit - Bestimmung der Gentoxizität mit
mesurage de l'induction de micronoyaux - Partie 2: dem In-vitro-Mikrokerntest - Teil 2: Verwendung einer nicht-
Méthode de la population mélangée à l'aide de la lignée de synchronisierten V79-Zellkulturlinie (ISO 21427-2:2006)
cellules V79 (ISO 21427-2:2006)
This European Standard was approved by CEN on 1 March 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21427-2:2009: E
worldwide for CEN national Members.

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SIST EN ISO 21427-2:2009
EN ISO 21427-2:2009 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 21427-2:2009
EN ISO 21427-2:2009 (E)
Foreword
The text of ISO 21427-2:2006 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 21427-2:2009 by
Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2009, and conflicting national standards shall be
withdrawn at the latest by September 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 21427-2:2006 has been approved by CEN as a EN ISO 21427-2:2009 without an
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