Surface active agents - Quantitative determination of free fatty acid in alkylamidopropylbetaines - Gas-chromatographic method

This European Standard specifies a procedure for the determination of the content of free fatty acid, FFA, in alkylamidopropylbetaines, which is defined as being the amount of fatty acid expressed in grams per 100 g of product.
This method has been validated for the determination of fatty acids from C6 to C20 in a total concentration range from 0,02  g to more than 3,0 g fatty acid per 100 g of product.

Grenzflächenaktive Stoffe - Quantitative Bestimmung freier Fettsäure in Alkylamidopropylbetainen - Gaschromatographisches Verfahren

Diese Europäische Norm legt ein Verfahren zur Bestimmung des Massenanteils an freier Fettsäure, FFA, in
Alkylamidopropylbetainen fest, der als Menge an Fettsäure in Gramm je 100 g des Produkts definiert ist.
Validiert wurde dieses Verfahren zur Bestimmung von C6- bis C20-Fettsäuren in einem Gesamtkonzentrationsbereich
der Fettsäure je 100 g des Produkts von 0,02 g/100 g bis größer 3,0 g/100 g.

Agents de surface - Dosage quantitatif des acides gras libres dans les alkylamidopropylbétaïnes - Méthode par chromatographie en phase gazeuse

La présente Norme européenne spécifie un mode opératoire de détermination de la teneur en acides gras libres des alkylamidopropylbétaïnes, définie comme étant la quantité d’acides gras exprimée en grammes pour 100 g de produit.
La présente méthode a été validée pour le dosage des acides gras de C6 à C20 dans une plage de concentration totale allant de 0,02 g à plus de 3,0 g d’acides gras pour 100 g de produit.

Površinsko aktivne snovi - Kvantitativno določevanje prostih maščobnih kislin v alkilamidopropilbetainih - Metoda plinske kromatografije

General Information

Status
Published
Publication Date
06-May-2008
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Due Date
07-May-2008
Completion Date
07-May-2008

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SLOVENSKI STANDARD
SIST EN 15608:2008
01-julij-2008
3RYUãLQVNRDNWLYQHVQRYL.YDQWLWDWLYQRGRORþHYDQMHSURVWLKPDãþREQLKNLVOLQY
DONLODPLGRSURSLOEHWDLQLK0HWRGDSOLQVNHNURPDWRJUDILMH
Surface active agents - Quantitative determination of free fatty acid in
alkylamidopropylbetaines - Gas-chromatographic method
Grenzflächenaktive Stoffe - Quantitative Bestimmung freier Fettsäure in
Alkylamidopropylbetainen - Gaschromatographisches Verfahren
Agents de surface - Dosage quantitatif des acides gras libres dans les
alkylamidopropylbétaïnes - Méthode par chromatographie en phase gazeuse
Ta slovenski standard je istoveten z: EN 15608:2008
ICS:
71.100.40 Površinsko aktivna sredstva Surface active agents
SIST EN 15608:2008 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
EUROPEAN STANDARD
EN 15608
NORME EUROPÉENNE
EUROPÄISCHE NORM
May 2008
ICS 71.100.40
English Version
Surface active agents - Quantitative determination of free fatty
acid in alkylamidopropylbetaines - Gas-chromatographic method

Agents de surface - Dosage quantitatif des acides gras Grenzflächenaktive Stoffe - Quantitative Bestimmung freier

libres dans les alkylamidopropylbétaïnes - Méthode par Fettsäure in Alkylamidopropylbetainen -

chromatographie en phase gazeuse Gaschromatographisches Verfahren
This European Standard was approved by CEN on 11 April 2008.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the

official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels

© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15608:2008: E

worldwide for CEN national Members.
---------------------- Page: 2 ----------------------
EN 15608:2008 (E)
Contents

Foreword......................................................................................................................................................................3

1 Scope ..............................................................................................................................................................4

2 Normative references ....................................................................................................................................4

3 Principle..........................................................................................................................................................4

4 Reagents.........................................................................................................................................................4

5 Apparatus .......................................................................................................................................................5

6 Sampling and preparation of the test sample.............................................................................................5

7 Procedure .......................................................................................................................................................5

8 Calculation and expression of results.........................................................................................................6

9 Precision.........................................................................................................................................................6

10 Test report ......................................................................................................................................................7

Annex A (informative) Reference GLC Chromatogram .........................................................................................8

A.1 GC Conditions................................................................................................................................................8

Annex B (informative) Method validation................................................................................................................9

B.1 Calibration ......................................................................................................................................................9

B.2 Statistical and other data derived from the results of interlaboratory tests ...........................................9

B.3 Remarks........................................................................................................................................................10

Bibliography ..............................................................................................................................................................11

---------------------- Page: 3 ----------------------
EN 15608:2008 (E)
Foreword

This document (EN 15608:2008) has been prepared by Technical Committee CEN/TC 276 “Surface active

agents”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by November 2008, and conflicting national standards shall be withdrawn

at the latest by November 2008.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech

Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,

Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,

Sweden, Switzerland and the United Kingdom.
---------------------- Page: 4 ----------------------
EN 15608:2008 (E)
1 Scope

This European Standard specifies a procedure for the determination of the content of free fatty acid, FFA, in

alkylamidopropylbetaines, which is defined as being the amount of fatty acid expressed in grams per 100 g of

product.

This method has been validated for the determination of fatty acids from C to C in a total concentration

6 20
range from 0,02 g to more than 3,0 g fatty acid per 100 g of product.
2 Normative references

The following referenced documents are indispensable for the application of this document. For dated

references, only the edition cited applies. For undated references, the latest edition of the referenced

document (including any amendments) applies.

EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)

ISO 607, Surface active agents and detergents – Methods of sample division
3 Principle

The free fatty acids are extracted with petroleum ether at acidic pH. Then the extracted fatty acids are

derivatised and subsequently analysed by GLC-FID. The chromatogram resolves the different acids according

to their alkyl chain length. For quantification the sum of the peak areas of all fatty acid homologues is related

to the peak area of the internal standard tridecanoic acid.
4 Reagents
4.1 General

WARNING — Your attention is drawn to the regulations covering the handling of hazardous

substances. Technical, organisational and personal protection measures should be observed.

During the analysis, unless otherwise specified, use only reagents of recognized analytical grade and that

have been checked in advance as to not interfere with the analytical results and water complying with grade 1

as defined in EN ISO 3696.
4.2 Tridecanoic acid, purity ≥ 99 % (m/m) (CAS number: 638-53-9).
4.3 Petroleum ether (40 ºC to 60 ºC) (CAS number: 101316-46-5).
4.4 Ethanol (CAS number: 64-17-5).
4.5 HCl, c = 37% (m/m) (CAS number: 7647-01-0).
4.6
...

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