EN 17715:2024
(Main)Plant biostimulants - Detection of Shigella spp.
Plant biostimulants - Detection of Shigella spp.
This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest percentage in the blend, the European Standard for the highest percentage of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
Dieses Dokument liefert ein Verfahren zum Nachweis, dass das Pathogen Shigella spp. in mikrobiellen Pflanzen-Biostimulanzien nicht vorhanden ist.
Das Nachweisverfahren für Shigella-Pathogene ist nicht trennscharf, und eine Quantifizierung wird selten durchgeführt. Der Nachweis erfolgt in der Regel mit einem Anreicherungsmedium und anschließender Subkultivierung auf verschiedenen Selektivmedien.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien prozentual den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Biostimulants des végétaux - Recherche de Shigella spp.
Le présent document fournit une méthode permettant de vérifier que le pathogène Shigella spp. n’est pas présent dans les biostimulants microbiens des végétaux.
La méthode de recherche des pathogènes Shigella n’est pas sensible et la quantification est rarement réalisée. La recherche est généralement réalisée en utilisant un milieu d’enrichissement, puis en procédant à un repiquage sur une variété de milieux sélectifs.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le pourcentage le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le pourcentage le plus élevé du mélange, la Norme européenne relative au composant représentant le pourcentage le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17715:2023
Rastlinski biostimulanti - Ugotavljanje prisotnosti Shigella spp.
Plant biostimulants - Detection of Shigella spp.
Pflanzen-Biostimulanzien - Nachweis von Shigella spp.
Biostimulants des végétaux - Recherche de Shigella spp.
Ta slovenski standard je istoveten z: EN 17715:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17715
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17715:2022
English Version
Plant biostimulants - Detection of Shigella spp.
Biostimulants des végétaux - Recherche de Pflanzen-Biostimulanzien - Nachweis von
Shigella spp. Shigella spp.
This European Standard was approved by CEN on 26 August 2024.
This European Standard was corrected and reissued by the CEN-CENELEC Management Centre on 11 December 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17715:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Culture media, reagents and antisera . 7
6 Apparatus and glassware . 7
7 Sampling . 7
8 Procedure. 7
9 Expression of results . 14
10 Test report . 14
Annex A (normative) Diagram of test procedure . 16
Annex B (normative) Composition and preparation of culture media and reagents . 17
Annex C (normative) Description of Shigella colony morphology and colour on selective
agars, for both identification and quality control purposes . 28
Annex D (informative) Interlaboratory study . 29
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilising products aimed to be covered. 32
Bibliography . 33
European foreword
This document (EN 17715:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17715:2022.
— the introduction has been updated and Table 1 has been removed;
— Clause 2, Normative references, has been updated;
— Annex B and Annex C have been revised;
— Annex D on the interlaboratory study has been added;
— Annex ZA has been added.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
For the relationship with EU Legislation, see informative Annex ZA, which is an integral part of this
document.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
WARNING — Persons using this document should be familiar with normal laboratory practice. This
document does not purport to address all of the safety problems, if any, associated with its use. It is the
responsibility of the user to establish appropriate safety and health practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document be
carried out by suitably trained staff.
1 Scope
This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial
plant biostimulants.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed.
Detection is usually performed using an enrichment medium followed by subculturing onto a variety of
selective media.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Plant Biostimulants and where the following category Plant Biostimulants is the highest
percentage in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant
Biostimulants is not the highest percentage in the blend, the European Standard for the highest
percentage of the blend applies. In case a blend of fertilizing products is composed of components in
equal quantity or in case the component EU fertilising products used for the blend have identical
formulations , the user decides which standard to apply.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17708:2024, Plant biostimulants — Preparation of sample for microbial analysis
EN ISO 21567:2004, Microbiology of food and animal feeding stuffs — Horizontal method for the detection
of Shigella spp. (ISO 21567:2004)
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN ISO 11133:2014 , Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media (ISO 11133:2014)
3 Terms and definitions
For the purposes of this document, the terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
• ISO Online browsing platform: available at https://www.iso.org/obp
• IEC Electropedia: available at https://www.electropedia.org/
3.1
Shigella spp.
microorganisms which form typical colonies on solid selective media described and which display the
morphological, physiological and biochemical characteristics described when the analysis is carried out
in accordance with this document
An example of such a blend is a product with 2 claimed functions consisting of a non-microbial plant biostimulant
and an organic fertilizer composed of 1 kg/kg of plant biostimulant from
seaweed.
As impacted by EN ISO 11133:2014/A1:2018 and EN ISO 11133:2014/A2:2020.
3.2
detection of Shigella spp.
determination of the presence or absence of these microorganisms in a particular mass of product, when
tests are carried out in accordance with this document
3.3
interlaboratory study
study performed by multiple laboratories testing identical samples at the same time, the results of which
are used to estimate alternative-method performance parameters
Note 1 to entry: The aim of an interlaboratory study is to determine the variability of the results obtained in
different laboratories using identical samples.
[SOURCE: ISO 16140-1:2016, 2.33 [2]]
3.4
sensitivity
number of samples found positive divided by the number of samples tested at a given level of
contamination
Note 1 to entry: The results are thus dependent on the level of contamination of the sample.
3.5
specificity
number of samples found negative divided by the number of blank samples tested
3.6
positive predictive value
PPV
ratio of positive samples identified as positive to all those with positive test results (including samples
that were incorrectly identified as positive)
3.7
negative predictive value
NPV
ratio of negative samples identified as negative to all those with negative test results (including samples
that were incorrectly identified as negative)
4 Principle
4.1 General
Detection of Shigella spp. shall be conducted in accordance with the sections specified in
EN ISO 21567:2004 and with the following four successive stages (according to Annex A, Figure A.1).
4.2 Enrichment in selective liquid medium
A test portion shall be inoculated with Shigella broth (B.2.1) containing 0,5 µg/ml of novobiocin, then
incubated anaerobically at 41,5 °C ± 1°C for 16 h to 20 h.
4.3 Plating out and identification of colonies
From the enrichment culture obtained, three selective differential media shall be inoculated: MacConkey
agar with low selectivity; XLD agar with moderate selectivity; Hektoen enteric agar with the greatest
selectivity. All shall be incubated at 37 °C ± 1°C for 20 h to 24 h.
4.4 Biochemical and serological confirmation
Typical and suspect colonies shall be selected from each of the three selective agars. The colonies shall
be purified on nutrient agar, then biochemical and serological characterizations shall be carried out using
the tests described.
5 Culture media, reagents and antisera
Use only reagents of recognized analytical grade, unless otherwise specified, and di
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