EN ISO 6878:2004
(Main)Water quality - Determination of phosphorus - Ammonium molybdate spectrometric method (ISO 6878:2004)
Water quality - Determination of phosphorus - Ammonium molybdate spectrometric method (ISO 6878:2004)
ISO 6878:2004 specifies methods for the determination of orthophosphate, orthophosphate after solvent extraction, hydrolysable phosphate plus orthophosphate, and total phosphorus after decomposition.
The methods are applicable to all kinds of water including seawater and effluents. Phosphorus concentrations within the range of 0,005 mg/l to 0,8 mg/l may be determined in such samples without dilution.
A solvent extraction procedure allows smaller phosphorus concentrations to be determined with a detection limit of about 0,000 5 mg/l.
Wasserbeschaffenheit - Bestimmung von Phosphur - Photometrisches Verfahren mittels Ammoniummolybdat (ISO/FDIS 6878:2004)
Diese Internationale Norm legt Verfahren für die Bestimmung von
- Orthophosphat (siehe Abschnitt 4);
- Orthophosphat nach Lösemittel-Extraktion (siehe Abschnitt 5);
- hydrolysierbarem Phosphat und Orthophosphat (siehe Abschnitt 6);
- Gesamtphosphor nach Aufschluss (siehe Abschnitte 7 und 8) fest.
Die Verfahren sind auf alle Wasserarten, einschließlich Meerwasser und Abwässer, anwendbar. Ohne Verdünnung der Proben können Phosphor-Konzentrationen im Bereich von 0,005 mg/l bis 0,8 mg/l Phosphor bestimmt werden.
Ein Extraktionsschritt gestattet es, niedrigere Phosphorkonzentrationen mit einer Nachweisgrenze von etwa 0,000 5 mg/l zu bestimmen.
Qualité de l'eau - Dosage du phosphore - Méthode spectrométrique au molybdate d'ammonium (ISO 6878:2004)
L'ISO 6878:2004 spécifie des méthodes de dosage des orthophosphates, des orthophosphates après extraction au solvant, des phosphates hydrolysables et des orthophosphates, et du phosphore total après décomposition.
Ces méthodes sont applicables à tous les types d'eau, y compris l'eau de mer et les effluents. Des concentrations en phosphore comprises entre 0,005 mg/l et 0,8 mg/l peuvent être déterminées sans dilution pour ces échantillons.
Un mode opératoire d'extraction au solvant permet de déterminer des concentrations en phosphore plus faibles avec une limite de détection d'environ 0,000 5 mg/l.
Kakovost vode - Določevanje fosforja - Spektrometrijska metoda z amonijevim molibdatom (ISO 6878:2004)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2004
1DGRPHãþD
SIST EN 1189:1997
.DNRYRVWYRGH'RORþHYDQMHIRVIRUMD6SHNWURPHWULMVNDPHWRGD]DPRQLMHYLP
PROLEGDWRP ,62
Water quality - Determination of phosphorus - Ammonium molybdate spectrometric
method (ISO 6878:2004)
Wasserbeschaffenheit - Bestimmung von Phosphur - Photometrisches Verfahren mittels
Ammoniummolybdat (ISO 6878:2004)
Qualité de l'eau - Dosage du phosphore - Méthode spectrométrique au molybdate
d'ammonium (ISO 6878:2004)
Ta slovenski standard je istoveten z: EN ISO 6878:2004
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 6878
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2004
ICS 13.060.50 Supersedes EN 1189:1996
English version
Water quality - Determination of phosphorus - Ammonium
molybdate spectrometric method (ISO 6878:2004)
Qualité de l'eau - Dosage du phosphore - Méthode
spectrométrique au molybdate d'ammonium (ISO
6878:2004)
This European Standard was approved by CEN on 21 May 2004.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2004 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6878:2004: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 6878:2004) has been prepared by Technical Committee ISO/TC 147
"Water quality" in collaboration with Technical Committee CEN/TC 230 "Water analysis", the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by December 2004, and conflicting national
standards shall be withdrawn at the latest by December 2004.
This document supersedes EN 1189:1996.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 6878:2004 has been approved by CEN as EN ISO 6878:2004 without any
modifications.
INTERNATIONAL ISO
STANDARD 6878
Second edition
2004-06-01
Water quality — Determination of
phosphorus — Ammonium molybdate
spectrometric method
Qualité de l'eau — Dosage du phosphore — Méthode spectrométrique
au molybdate d'ammonium
Reference number
ISO 6878:2004(E)
©
ISO 2004
ISO 6878:2004(E)
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ii © ISO 2004 – All rights reserved
ISO 6878:2004(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Interferences. 1
3 Principle . 1
4 Determination of orthophosphate . 2
5 Determination of orthophosphate after solvent extraction . 7
6 Determination of hydrolysable phosphate and orthophosphate. 9
7 Determination of total phosphorus after peroxodisulfate oxidation . 11
8 Determination of total phosphorus after nitric acid-sulfuric acid digestion . 14
Annex A (informative) Interferences. 17
Annex B (informative) Precision data. 19
Bibliography . 21
ISO 6878:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6878 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,
chemical and biochemical methods.
This second edition cancels and replaces the first edition (ISO 6878:1998), which has been technically revised.
iv © ISO 2004 – All rights reserved
ISO 6878:2004(E)
Introduction
This International Standard specifies the determination of different forms of phosphorus compounds present in
ground, surface and waste waters in various concentrations in the dissolved and undissolved state.
The user should be aware that particular problems could require the specification of additional marginal
conditions.
INTERNATIONAL STANDARD ISO 6878:2004(E)
Water quality — Determination of phosphorus — Ammonium
molybdate spectrometric method
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions. It is absolutely essential
that tests conducted according to this International Standard be carried out by suitably qualified staff.
Molybdate and antimony waste solutions should be disposed of properly.
1 Scope
This International Standard specifies methods for the determination of
orthophosphate (see Clause 4);
orthophosphate after solvent extraction (see Clause 5);
hydrolysable phosphate plus orthophosphate (see Clause 6);
total phosphorus after decomposition (see Clauses 7 and 8).
The methods are applicable to all kinds of water including seawater and effluents. Phosphorus concentrations
within the range of 0,005 mg/l to 0,8 mg/l may be determined in such samples without dilution.
A solvent extraction procedure allows smaller phosphorus concentrations to be determined with a detection
limit of about 0,000 5 mg/l.
2 Interferences
See Annex A for some known interferences. There may be others and it is recommended to verify whether
any such interferences exist and take action to eliminate them.
3 Principle
Reaction of orthophosphate ions with an acid solution containing molybdate and antimony ions to form an
antimony phosphomolybdate complex.
Reduction of the complex with ascorbic acid to form a strongly coloured molybdenum blue complex.
Measurement of the absorbance of this complex to determine the concentration of orthophosphate present.
Polyphosphate and some organophosphorus compounds are determined if converted to molybdate reactive
orthophosphate formed by sulfuric acid hydrolysis.
Many organophosphorus compounds are converted to orthophosphate by mineralization with peroxodisulfate.
Nitric acid-sulfuric acid mineralization is used if a more vigorous treatment is required.
ISO 6878:2004(E)
4 Determination of orthophosphate
4.1 Reagents
During the analysis, use only reagents of recognized analytical grade and only water having a phosphate
content that is negligible compared with the lowest concentration to be determined in the samples.
For low phosphate contents, double-distilled water from an all-glass apparatus is recommended.
4.1.1 Sulfuric acid solution, c(H SO ) ≈ 9 mol/l.
2 4
Add 500 ml ± 5 ml of water to a 2 l beaker. Cautiously add, with continuous stirring and cooling, 500 ml ± 5 ml
of sulfuric acid, ρ = 1,84 g/ml. Mix well and allow the solution to cool to room temperature.
4.1.2 Sulfuric acid solution, c(H SO ) ≈ 4,5 mol/l.
2 4
Add 500 ml ± 5 ml of water to a 2 l beaker. Cautiously add, with continuous stirring and cooling, 500 ml ± 5 ml
of sulfuric acid (4.1.1). Mix well and allow to cool to room temperature.
4.1.3 Sulfuric acid solution, c(H SO ) ≈ 2 mol/l.
2 4
Add 300 ml ± 3 ml of water to a 1 l beaker. Cautiously add 110 ml ± 2 ml of sulfuric acid solution (4.1.1), with
continuous stirring and cooling. In a measuring flask, dilute to 500 ml ± 2 ml with water and mix well.
4.1.4 Sodium hydroxide solution, c(NaOH) = 2 mol/l.
Dissolve 80 g ± 1 g of sodium hydroxide pellets in water, cool and dilute to 1 l with water.
4.1.5 Ascorbic acid solution, ρ = 100 g/l.
Dissolve 10 g ± 0,5 g of ascorbic acid (C H O ) in 100 ml ± 5 ml water.
6 8 6
NOTE The solution is stable for 2 weeks if stored in an amber glass bottle in a refrigerator and can be used as long
as it remains colourless.
4.1.6 Acid molybdate, Solution I.
Dissolve 13 g ± 0,5 g of ammonium heptamolybdate tetrahydrate [(NH ) Mo O ⋅4H O] in 100 ml ± 5 ml of
4 6 7 24 2
water. Dissolve 0,35 g ± 0,05 g of antimony potassium tartrate hemihydrate [K(SbO)C H O ⋅½H O] in
4 4 6 2
100 ml ± 5 ml of water.
Add the molybdate solution to 300 ml ± 5 ml of sulfuric acid (4.1.1) with continuous stirring. Add the tartrate
solution and mix well.
NOTE The reagent is stable for at least 2 months if stored in an amber glass bottle.
4.1.7 Acid molybdate, Solution II.
Cautiously add 230 ml ± 0,5 ml of sulfuric acid (4.1.1) to 70 ml ± 5 ml of water, cool. Dissolve 13 g ± 0,5 g of
ammonium heptamolybdate tetrahydrate [(NH ) Mo O ⋅4H O] in 100 ml ± 5 ml of water. Add to the acid
4 6 7 24 2
solution and mix well. Dissolve 0,35 g ± 0,05 g of antimony potassium tartrate hemihydrate
[K(SbO)C H O ⋅½H O] in 100 ml ± 5 ml of water. Add to the molybdate-acid solutio
...
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