EN ISO 21872-1:2017

Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)

ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
ISO 21872-1:2017 is applicable to the following:
-      products intended for human consumption and the feeding of animals;
-      environmental samples in the area of food production and food handling.

Mikrobiologie der Lebensmittelkette — Horizontales Verfahren zur Bestimmung von Vibrio spp. — Teil 1: Nachweis von potentiell enteropathenogenen Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus (ISO 21872-1:2017)

Dieses Dokument legt ein horizontales Verfahren zum Nachweis von enteropathogenen Vibrio spp. fest, die Erkrankungen des Menschen am oder über den Darmtrakt verursachen. Die Spezies, deren Nachweis die hier aufgeführten Verfahren einbeziehen, sind Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus.
Das Dokument gilt für
—   Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind;
—   Umgebungsproben im Bereich der Herstellung von Lebensmitteln und beim Umgang mit Lebensmitteln.
ANMERKUNG 1   Dieses Verfahren ist für bestimmte Produkte nicht unbedingt bis ins Detail geeignet (siehe Einleitung).
ANMERKUNG 2   Die Weltgesundheitsorganisation (WHO; en: World Health Organization) hat V. parahaemolyticus, V. cholerae und V. vulnificus als die wichtigsten lebensmittelassoziierten Vibrio spp. identifiziert. Das Verfahren in diesem Dokument kann jedoch ebenso für die Identifizierung anderer Vibrio spp., die Erkrankungen des Menschen verursachen, geeignet sein [1].

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la détermination des Vibrio spp. - Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus potentiellement entéropathogènes (ISO 21872-1:2017)

ISO 21872-1:2017 spécifie une méthode horizontale pour la recherche des espèces entéropathogènes de Vibrio provoquant des maladies dans ou via le tractus intestinal chez l'homme. Les espèces détectables par les méthodes spécifiées incluent Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus.
ISO 21872-1:2017 s'applique:
-      aux produits destinés à la consommation humaine et à l'alimentation animale;
-      aux échantillons environnementaux dans le domaine de la production et de la manutention de denrées alimentaires.

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje Vibrio spp. - 1. del: Ugotavljanje potencialno enteropatogene Vibrio parahaemolyticus, Vibrio cholerae in Vibrio vulnificus (ISO 21872-1:2017)

Ta standard opisuje ugotavljanje patogenih vrst Vibrio parahaemolyticus in Vibrio cholerae (referenčni dokument je standard ISO/TS 21872 -1).

General Information

• Status: Published
• Publication Date: 11-Jul-2017
• Withdrawal Date: 30-Jan-2018
• ICS: 07.100.30 - Food microbiology
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 12-Jul-2017
• Due Date: 01-Mar-2014
• Completion Date: 12-Jul-2017
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/381 - Mandate for standardisation addressed to CEN in the field of Methods of analysis of foodstuffs concerning Food Hygiene
• Ref Project: SIST EN ISO 21872-1:2017 - Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)

Relations

• Amended By: EN ISO 21872-1:2017/A1:2023 - Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus - Amendment 1: Inclusion of performance testing of culture media and reagents (ISO 21872 1:2017/Amd 1:2023)
• Effective Date: 25-Nov-2020

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2017
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje Vibrio
spp. - 1. del: Ugotavljanje potencialno enteropatogene Vibrio parahaemolyticus,
Vibrio cholerae in Vibrio vulnificus (ISO 21872-1:2017)
Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. -
Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio
cholerae and Vibrio vulnificus (ISO 21872-1:2017)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum
Nachweis von potentiell enteropathogenen Vibrio spp. - Teil 1: Nachweis von vibrio
parahaemolyticus und vibrio cholerae (ISO 21872-1:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la détermination des
Vibrio spp. - Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio
cholerae et Vibrio vulnificus potentiellement entéropathogènes (ISO 21872-1:2017)
Ta slovenski standard je istoveten z: EN ISO 21872-1:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21872-1
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2017
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - Horizontal
method for the detection of potentially enteropathogenic
Vibrio spp. - Part 1: Detection of Vibrio parahaemolyticus
and Vibrio cholerae (ISO 21872-1:2017)
Microbiologie des aliments - Méthode horizontale pour Mikrobiologie von Lebensmitteln und Futtermitteln -
la recherche des Vibrio spp. potentiellement Horizontales Verfahren zum Nachweis von potentiell
entéropathogènes - Partie 1: Recherche de Vibrio enteropathogenen Vibrio spp. - Teil 1: Nachweis von
parahaemolyticus et Vibrio cholerae (ISO 21872- vibrio parahaemolyticus und vibrio cholerae (ISO
1:2017) 21872-1:2017)
This European Standard was approved by CEN on 14 May 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21872-1:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 21872-1:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2018 and conflicting national standards shall
be withdrawn at the latest by January 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 21872-1:2017 has been approved by CEN as EN ISO 21872-1:2017 without any
modification.
INTERNATIONAL ISO
STANDARD 21872-1
First edition
2017-06
Microbiology of the food chain —
Horizontal method for the
determination of Vibrio spp. —
Part 1:
Detection of potentially
enteropathogenic Vibrio
parahaemolyticus, Vibrio cholerae and
Vibrio vulnificus
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la détermination des Vibrio spp. —
Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio
cholerae et Vibrio vulnificus potentiellement entéropathogènes
Reference number
ISO 21872-1:2017(E)
©
ISO 2017
ISO 21872-1:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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ii © ISO 2017 – All rights reserved

ISO 21872-1:2017(E)
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Principle . 2
4.1 General . 2
4.2 Primary enrichment in a liquid selective medium . 2
4.3 Secondary enrichment in a liquid selective medium . 3
4.4 Isolation and identification . 3
4.5 Confirmation . 3
5 Culture media and reagents . 3
5.1 Enrichment medium: alkaline saline peptone water (ASPW). 4
5.2 Solid selective isolation media . 4
5.2.1 First medium: thiosulphate, citrate, bile and sucrose agar medium (TCBS) . 4
5.2.2 Second medium. 4
5.3 Saline nutrient agar (SNA) . 4
5.4 Reagent for detection of oxidase . 4
5.5 Biochemical tests . 4
5.5.1 L-lysine decarboxylase saline medium (LDC) . 4
5.5.2 Arginine dihydrolase saline medium (ADH) . 4
5.5.3 Reagent for detection of β-galactosidase . 5
5.5.4 Saline medium for detection of indole . 5
5.5.5 Saline peptone waters . 5
5.5.6 Sodium chloride solution . 5
5.6 PCR . 5
5.6.1 Tris acetate EDTA buffer (TAE) (or a buffer allowing similar performance
for the purpose) . 5
5.6.2 Mastermix . 5
5.6.3 Primers and probes . 5
5.6.4 Positive control material . 5
5.6.5 Negative extraction control . 6
6 Equipment and consumables . 6
7 Sampling . 6
8 Preparation of the test sample . 6
9 Procedure (See Figure A.1) . 7
9.1 Test portion and initial suspension . 7
9.2 Primary selective enrichment . 7
9.3 Secondary selective enrichment . 7
9.4 Isolation and identification . 8
9.5 Confirmation . 8
9.5.1 General. 8
9.5.2 Selection of colonies for confirmation and preparation of pure cultures .
...