EN 16772:2016
(Main)Water quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers
Water quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers
This European Standard provides guidance on methods for sampling invertebrates in the hyporheic zone of wadable rivers. It describes each method, including details of the equipment involved and its use in the field. Guidance is given on developing a sampling strategy and selecting an appropriate survey technique for the purpose of investigation.
NOTE Benthic macroinvertebrate sampling is covered by other published standards (see Bibliography). Selected literature with references in support of this document is given in the Bibliography.
Wasserbeschaffenheit - Anleitung für Verfahren zur Probenahme von Invertebraten in der hyporheischen Zone von Flüssen
Qualité de l'eau - Lignes directrices relatives aux méthodes d'échantillonnage des invertébrés dans la zone hyporhéique des rivières
La présente Norme européenne fournit des lignes directrices relatives à l’échantillonnage des invertébrés dans la zone hyporhéique de rivières prospectables à pied. Elle décrit chaque méthode, y compris les détails du matériel nécessaire et son utilisation sur le terrain. Des lignes directrices sont données sur l’élaboration d’une stratégie d’échantillonnage et la sélection d’une technique de sondage appropriée pour l’étude.
NOTE L’échantillonnage de macro-invertébrés benthiques est traité dans d’autres normes publiées (voir la Bibliographie). La littérature sélectionnée dont les références étayent le présent document est indiquée dans la Bibliographie.
Kakovost vode - Navodilo za metode vzorčenja nevretenčarjev v hiporeični coni rek
Ta dokument podaja navodila za metode vzorčenja nevretenčarjev v hiporhaičnih conah prebrodljivih rek. Opisuje vsako metodo, vključno s podrobnostmi o vključeni opremi in njeni uporabi na terenu. Podana so navodila o razvijanju strategije vzorčenja in izbiranju ustrezne tehnike raziskovanja za namen preiskave. Vzorčenje bentoških makronevretenčarjev je obravnavano v drugih objavljenih standardih (glej bibliografijo). Izbrana literatura s sklici v podporo temu dokumentu je podana v bibliografiji.
General Information
Overview
EN 16772:2016 - Water quality: Guidance on methods for sampling invertebrates in the hyporheic zone of rivers (CEN) - provides practical, field-focused guidance for collecting and assessing invertebrate fauna living within the saturated sediments beneath and alongside wadable river channels (the hyporheic zone). The standard describes sampling equipment and field procedures, advises on developing a sampling strategy, and helps users select appropriate survey techniques for specific study objectives. It complements, but does not replace, standards for benthic macroinvertebrate sampling.
Key topics and requirements
- Scope and definitions: Clear definitions for hyporheic terms (hyporheic zone, hyporheos, benthos, meiofauna, macroinvertebrate) and the standard’s focus on wadable rivers.
- Survey objectives: Emphasises defining aims before method selection; suitability of methods depends on whether the study targets diversity, abundance, biomass, or impact assessments.
- Sampling strategy: Guidance on designing spatial and temporal sampling (reach selection, substrate types, instream vs riparian locations) and on quantitative vs semi‑quantitative approaches.
- Detailed sampling methods: Descriptions and field use of techniques and equipment, including (as covered in the standard):
- Karaman‑Chappuis pit
- Bou‑Rouch pump
- Vacuum pump
- Standpipe trap
- Williams corer
- Colonization devices
- Freeze coring Each method includes notes on species typically sampled and relevant environmental variables.
- Sample processing: Field and laboratory handling recommendations to ensure data quality.
- Safety and adaptability: Emphasises health & safety compliance and that methods may need modification for local conditions.
Practical applications
- River monitoring and ecological status assessments under water‑quality programmes
- Catchment management, river restoration and impact studies (pollution, habitat alteration, fish spawning nursery evaluation)
- Scientific research into hyporheic biodiversity, biogeochemical processes and surface–groundwater interactions
- Consultants and environmental contractors designing targeted invertebrate sampling campaigns
Who should use it
- Environmental agencies and regulators
- Hydrogeologists and stream ecologists
- Water quality scientists and biodiversity researchers
- Environmental consultants and field survey teams
Related standards and policy links
- Complements standards for benthic macroinvertebrate sampling (see Bibliography)
- Relevant EU legislation referenced in the standard: Habitats Directive, Water Framework Directive, Groundwater Directive, Nitrates Directive
- For detailed taxonomic or laboratory protocols consult the standard’s Bibliography and national guidance
Keywords: EN 16772, hyporheic zone, hyporheos, sampling invertebrates, water quality, CEN standard, river monitoring, sampling methods.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.UHNWasserbeschaffenheit - Anleitung für die Probenahme von Invertebraten (Wirbellosen) in der hyporheischen Zone von FlüssenQualité de l'eau - Lignes directrices relatives aux méthodes d'échantillonnage des invertébrés dans la zone hyporhéique de rivièresWater quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 16772:2016SIST EN 16772:2016en,fr,de01-december-2016SIST EN 16772:2016SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16772
April
t r s x ICS
s uä r x rä y r English Version
Water quality æ Guidance on methods for sampling invertebrates in the hyporheic zone of rivers Qualité de l 5eau æ Lignes directrices relatives aux méthodes d 5échantillonnage des invertébrés dans la zone hyporhéique des rivières
Wasserbeschaffenheit æ Anleitung zu Methoden für die hyporheischen Zone von Flüssen This European Standard was approved by CEN on
t r February
t r s xä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x y y tã t r s x ESIST EN 16772:2016
Examples of sampling strategies for three different types of investigation . 21 A.1 Case Study 1 – Assessing regional biodiversity and species richness . 21 A.2 Case Study 2 – Assessing impacts on fish spawning sites . 21 A.3 Case Study 3 – Assessing the impacts of pollution . 21 Bibliography . 22
Karaman-Chappuis pit Bou-Rouch pump Vacuum pump Standpipe trap Williams corer Colonization devices Freeze coring Migration/dispersal No No No Yes No Yes No Spatial heterogeneity No Yes Yes Yes Yes Yes Yes Temporal variability Yes Yes Yes Yes Yes Yes Yes Interstitial sediment transport No No No Yes No Yes No Substrate characteristics No No No No No No Yes Used on submerged substrate No Yes Yes Yes Yes Yes Yes Used in riparian zone Yes Yes Yes Yes Yes Yes Yes Quantitative No No No No Yes Yes Yes Semi-quantitative No Yes Yes Yes Yes No No 4 Sampling strategy The design of a sampling strategy will vary according to the purpose of the investigation. Sampling site location may be influenced by pre-existing monitoring networks or previous investigations. The following should be considered: — sampling method; — number and location of sampling sites; — number of replicates per site required to characterize site heterogeneity (e.g. upwelling and downwelling, substrate characteristics); — sampling frequency; — sampling depth; — seasonal variability; — abiotic data requirements; — spatial and temporal scale of investigation. Scale is important when examining the hyporheic zone, as various processes occur at different spatial scales. For example, at a stream-bed (patch) scale the size, shape, sorting and stability of unconsolidated material are the primary determinants of porosity and permeability. These factors have a major influence on community composition over relatively short distances and methods have been developed to address this [11]. At a broader scale, lateral connectivity (e.g. between the riparian zone and out to the wider floodplain) is a key consideration. Hyporheic flow paths occur at multiple scales, from the stream bed to the catchment. SIST EN 16772:2016
Karaman-Chappuis pit Bou-Rouch pump Vacuum pump Stand-pipe trap Williams corer Coloniz-ation devices Freeze coring Minimum number of operators a 1 1 1 1 1 1 2 Equipment cost
(in Euro, 2016) < 200 < 1 000 < 350 < 200 < 1 000 < 500 < 10 000 Installation time ~15 min ~15 min ~15 min ~2 h ~15 min ~1 h ~30 min Time to collect one sample ~5 min ~10 min ~10 min ~10 min ~5 min ~10 min ~45 min Portability High Medium High Medium High Medium Low Typical recovery time after installation None None None 2 weeks None Variable Variable Repeat sampling possible No No Yes Yes No Yes No a Installation of equipment for some methods will require additional help, i.e. for the standpipe traps, colonization devices and freeze coring. 5.2 Karaman-Chappuis pit 5.2.1 Description and operation This is a rapid, qualitative method for obtaining a hyporheic sample from exposed river sediments, particularly in gravel and sand [14]. A pit (~50 cm diameter) is excavated to such a depth that its base is below the water level. Interstitial flow into the pit is maintained by extracting water, using a hand pump SIST EN 16772:2016
Key 1 safety pin 9 bolt 2 lever 10 screen 3 upper piston valve 11 tip 4 liner 12 hand piston pump 5 body of the pump 13 mobile standpipe 6 lower piston 14 hyporheic zone 7 lower valve 15 standpipe with screen with 5-mm diameter ho
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Frequently Asked Questions
EN 16772:2016 is a standard published by the European Committee for Standardization (CEN). Its full title is "Water quality - Guidance on methods for sampling invertebrates in the hyporheic zone of rivers". This standard covers: This European Standard provides guidance on methods for sampling invertebrates in the hyporheic zone of wadable rivers. It describes each method, including details of the equipment involved and its use in the field. Guidance is given on developing a sampling strategy and selecting an appropriate survey technique for the purpose of investigation. NOTE Benthic macroinvertebrate sampling is covered by other published standards (see Bibliography). Selected literature with references in support of this document is given in the Bibliography.
This European Standard provides guidance on methods for sampling invertebrates in the hyporheic zone of wadable rivers. It describes each method, including details of the equipment involved and its use in the field. Guidance is given on developing a sampling strategy and selecting an appropriate survey technique for the purpose of investigation. NOTE Benthic macroinvertebrate sampling is covered by other published standards (see Bibliography). Selected literature with references in support of this document is given in the Bibliography.
EN 16772:2016 is classified under the following ICS (International Classification for Standards) categories: 13.060.70 - Examination of biological properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 16772:2016 is associated with the following European legislation: EU Directives/Regulations: 2000/60/EC. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
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SIST EN 16772:2016は、河川のヒポリヒクゾーンにおける無脊椎動物の採取方法に関するヨーロッパ標準であり、その適用範囲は非常に重要です。この標準は、歩行可能な河川のヒポリヒクゾーンにおける無脊椎動物のサンプリング手法に関する指針を提供しています。具体的には、各手法の詳細と使用する機器の情報、さらには実地での使用方法までを網羅しています。 この標準の強みは、無脊椎動物の採取に関する方法論を明確に定義している点にあります。サンプリング戦略を構築するためのガイダンスがあり、調査の目的に応じた適切な調査技術の選択を支援します。これにより、研究者や環境保護活動家は、ターゲットとする種やその生息環境に最適な方法を選ぶことが可能となります。 加えて、同標準では底生の大規模無脊椎動物サンプリングについては他の公開された標準に委ねている点にも注意が必要です。このように、EN 16772:2016は、ヒポリヒクゾーンにおける生態系の評価と監視を行う上で、非常に関連性の高い資料となっています。 このように、SIST EN 16772:2016は、河川のヒポリヒクゾーンにおける無脊椎動物のサンプリング手法に関する貴重な指針を提供し、実践者にとっての信頼できるリソースとなっています。
Die Norm EN 16772:2016 bietet wichtige Richtlinien für die Probenahme von wirbellosen Tieren in der hyporheischen Zone von Flüssen, insbesondere in begehbaren Gewässern. Diese europäische Norm stellt sicher, dass die herkömmlichen Methoden zur Probenahme standardisiert und somit reproduzierbar sind. Dies verbessert die Vergleichbarkeit von Daten zwischen verschiedenen Studien und Regionen erheblich. Ein herausragendes Merkmal der Norm ist die detaillierte Beschreibung der einzelnen Methoden zur Probenahme. Die Norm beinhaltet umfassende Informationen über das benötigte Equipment und dessen Anwendung im Feld. Dies ist besonders wertvoll für Forschende und Praktiker, die in der Gewässerökologie tätig sind, da es ihnen ermöglicht, qualitativ hochwertige und konsistente Proben zu entnehmen. Ein weiterer Stärke der EN 16772:2016 liegt in der Anleitung zur Entwicklung einer Probenahmestrategie sowie bei der Auswahl geeigneter Erhebungstechniken. Dies stellt sicher, dass die Probenahme nicht nur effizient, sondern auch zielgerichtet ist, sodass spezifische Forschungsfragen adäquat beantwortet werden können. Die Norm berücksichtigt zudem die unterschiedlichen Bedingungen in Fließgewässern, was ihre Anwendung in der Praxis noch wertvoller macht. Die Relevanz der EN 16772:2016 kann nicht genug betont werden, da sie eine zentrale Rolle im Bereich der Gewässeruntersuchungen spielt. Indem sie den Fokus auf die hyporheische Zone legt, die für die Biodiversität und die Gesundheit von Flüssen entscheidend ist, trägt diese Norm zur Förderung eines tieferen Verständnisses der aquatischen Ökosysteme bei. Insgesamt stellt die EN 16772:2016 einen bedeutenden Schritt in der Standardisierung von Sammlungsprozessen dar und fördert somit eine verbesserte ökologische Forschung und das Management von Flussökosystemen.
La norme EN 16772:2016 offre une approche essentielle pour l'évaluation de la qualité de l'eau, en fournissant des directives précises pour le prélèvement des invertébrés dans la zone hyporhéique des rivières praticables. Son champ d'application est particulièrement pertinent, car il couvre les méthodes de sampling adaptées à des environnements aquatiques spécifiques, permettant ainsi une collecte de données fiable pour une meilleure compréhension de la biodiversité aquatique. Les forces de cette norme résident dans sa clarté et la richesse des détails fournis. Chaque méthode de prélèvement est minutieusement décrite, avec des précisions sur l'équipement requis et son utilisation sur le terrain. Cela facilite la tâche des chercheurs et des gestionnaires de l'environnement en garantissant que les protocoles suivis sont harmonisés et fondés sur des pratiques éprouvées. De plus, EN 16772:2016 fournit des conseils sur l'élaboration d'une stratégie de prélèvement et la sélection de techniques d'enquête adéquates. Cela est crucial pour garantir que les études menées sont pertinentes et adaptées aux objectifs d'investigation, qu'ils soient liés à la conservation, à la restauration ou à l'évaluation de la santé des écosystèmes aquatiques. En somme, cette norme s'avère être un outil indispensable pour toute recherche ou gestion axée sur la qualité de l'eau et les écosystèmes fluviaux, en renforçant la capacité des acteurs à mener des évaluations rigoureuses et normalisées des invertébrés dans les zones hyporhéiques. Le lien avec la littérature et d'autres normes concernant le prélèvement de macroinvertébrés benthiques renforce également sa validité et son intégration dans le cadre plus large de l'écologie aquatique.
표준 EN 16772:2016은 강의 hyporheic zone에서 무척추동물을 샘플링하기 위한 방법론에 대한 지침을 제공합니다. 이 유럽 표준은 샘플링 방법을 상세히 설명하며, 현장에서의 장비 사용 및 그 세부사항을 명시합니다. 특히, 이 표준의 범위는 샘플링 전략 개발과 조사 목적에 맞는 적절한 조사 기법 선택에 대한 조언을 포함하고 있으며, 이는 수생 생태학의 최신 연구에 필수적인 요소입니다. EN 16772:2016의 강점 중 하나는 다양한 샘플링 방법을 체계적으로 정리하고 있다는 점입니다. 각 방법에서는 필요한 장비와 사용 방법에 대한 구체적인 설명이 이루어져 있어, 연구자가 실제 현장에서 쉽게 적용할 수 있도록 돕습니다. 이러한 접근은 수생 생태계의 건강성을 평가하고 관리하기 위한 기초 자료를 제공하는 데 매우 중요합니다. 또한, 이 표준은 경량화된 하수 구간 조사를 통해 무척추동물의 분포와 생태적 건강성을 이해하는 데 도움을 줄 수 있으며, 이는 환경 관리를 위한 전략 수립에 기여할 수 있습니다. EN 16772:2016은 또한 생물 다양성을 보존하고 회복하기 위한 조사 및 연구 프로젝트에 필수적인 지침을 제공하여, 환경 과학 연구자들에게 매우 유용한 자료입니다. 결론적으로, EN 16772:2016은 강의 hyporheic zone에서의 무척추동물 샘플링을 위한 필수 가이드라인으로, 환경 과학 및 생태학에 종사하는 연구자들에게 큰 가치를 제공합니다. 이 표준의 체계적이고 구체적인 접근은 향후 연구와 환경 관리를 위한 기반을 마련하는 데 기여할 것입니다.
The EN 16772:2016 standard is a comprehensive document that provides essential guidance on methods for sampling invertebrates in the hyporheic zone of rivers. This European Standard is particularly relevant for researchers, ecologists, and environmental scientists focused on assessing aquatic ecosystems. One of the key strengths of EN 16772:2016 lies in its detailed description of various sampling methods. The standard outlines specific techniques that can be employed in the field, offering clarity on the equipment needed for effective sampling. This specificity ensures that practitioners are well-informed, enabling accurate and consistent data collection from the hyporheic zone, which is an essential habitat for many aquatic invertebrates. Moreover, the EN 16772:2016 standard emphasizes the importance of developing a coherent sampling strategy. It guides users in selecting the most suitable survey techniques based on their investigation objectives. This aspect is particularly valuable, as it addresses the variability of river environments and the distinct characteristics of different study sites, facilitating adaptive and context-specific approaches to sampling. The standard also acknowledges the existing literature and other published standards related to benthic macroinvertebrate sampling, providing a well-rounded context for practitioners. This connection to established research in the field reinforces the credibility and relevance of EN 16772:2016, ensuring that users can integrate its guidance with broader ecological studies. In summary, EN 16772:2016 stands out for its in-depth methodology and practical guidance on sampling invertebrates in the hyporheic zone of wadable rivers. It serves as a crucial resource for those aiming to enhance their understanding of aquatic ecosystems and promotes the reliable collection of data necessary for effective environmental monitoring and research.
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