EN ISO 20227:2017

Water quality - Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock culture independent microbiotest (ISO 20227:2017)

ISO 20227:2017 specifies a method for the determination of the inhibition of the growth of the first fronds of Spirodela polyrhiza germinated from turions, by substances and mixtures contained in water or waste water, including treated municipal waste water and industrial effluents.
The test is also applicable to pure chemicals and in particular, plant protection products and pesticides.

Wasserbeschaffenheit - Bestimmung der wachstumshemmenden Wirkung von Abwässern, natürlichen Wässern und Chemikalien auf die Wasserlinsenart Spirodela polyrhiza - Verfahren mittels Stammkultur unabhängigem mikrobiologischem Test (ISO 20227:2017)

Qualité de l'eau - Détermination des effets d'inhibition sur la croissance de la lentille d'eau Spirodela polyrhiza par les eaux usées, les eaux naturelles et les produits chimiques - Méthode utilisant un bioessai miniaturisé indépendant d'une culture mère (ISO 20227:2017)

L'ISO 20227 :2017 spécifie une méthode permettant de déterminer l'inhibition de la croissance des premières frondes de Spirodela polyrhiza ayant germé à partir de turions, provoquée par des substances et des préparations contenues dans les eaux ou les eaux résiduaires, y compris les eaux résiduaires urbaines après traitement et les effluents industriels.
L'essai s'applique également aux produits chimiques purs et, en particulier, aux produits phytopharmaceutiques et aux pesticides.

Kakovost vode - Določevanje učinka odpadne vode, naravne vode in kemikalij na zaviranje rasti vodne leče Spirodela polyrhiza - Metoda z neodvisnim mikrobiološkim preskusom z založno kulturo (ISO 20227:2017)

Ta mednarodni standard določa metodo za določevanje zaviranja rasti prvih listov vodne leče Spirodela polyrhiza, ki vzkalijo iz mirujočih brstnih mešičkov, s snovmi in zmesmi, ki jih vsebuje voda ali odpadna voda, vključno s prečiščeno gospodinjsko odpadno vodo in industrijskimi odplakami. Preskus se uporablja tudi za čiste kemikalije, še posebej za izdelke za zaščito rastlin in pesticide.

General Information

• Status: Published
• Publication Date: 04-Jul-2017
• Withdrawal Date: 30-Jan-2018
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 05-Jul-2017
• Due Date: 16-Dec-2017
• Completion Date: 05-Jul-2017
• Ref Project: SIST EN ISO 20227:2017 - Water quality - Determination of the growth inhibition effects of waste waters, natural waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock culture independent microbiotest (ISO 20227:2017)

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2017
.DNRYRVWYRGH'RORþHYDQMHXþLQNDRGSDGQHYRGHQDUDYQHYRGHLQNHPLNDOLMQD
]DYLUDQMHUDVWLYRGQHOHþH6SLURGHODSRO\UKL]D0HWRGD]QHRGYLVQLP
PLNURELRORãNLPSUHVNXVRP]]DORåQRNXOWXUR,62
Water quality - Determination of the growth inhibition effects of waste waters, natural
waters and chemicals on the duckweed Spirodela polyrhiza - Method using a stock
culture independent microbiotest (ISO 20227:2017)
Wasserbeschaffenheit - Bestimmung der wachstumshemmenden Wirkung von
Abwässern, natürlichen Wässern und Chemikalien auf die Wasserlinsenart Spirodela
polyrhiza - Verfahren mittels Stammkultur unabhängigem mikrobiologischem Test (ISO
20227:2017)
Qualité de l'eau - Détermination des effets d'inhibition sur la croissance de la lentille
d'eau Spirodela polyrhiza par les eaux usées, les eaux naturelles et les produits
chimiques - Méthode utilisant un bioessai miniaturisé indépendant d'une culture mère
(ISO 20227:2017)
Ta slovenski standard je istoveten z: EN ISO 20227:2017
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 20227
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2017
EUROPÄISCHE NORM
ICS 13.060.70
English Version
Water quality - Determination of the growth inhibition
effects of waste waters, natural waters and chemicals on
the duckweed Spirodela polyrhiza - Method using a stock
culture independent microbiotest (ISO 20227:2017)
Qualité de l'eau - Détermination des effets d'inhibition Wasserbeschaffenheit - Bestimmung der
sur la croissance de la lentille d'eau Spirodela polyrhiza wachstumshemmenden Wirkung von Abwässern,
par les eaux usées, les eaux naturelles et les produits natürlichen Wässern und Chemikalien auf die
chimiques - Méthode utilisant un bioessai miniaturisé Wasserlinsenart Spirodela polyrhiza - Verfahren
indépendant d'une culture mère (ISO 20227:2017) mittels Stammkultur unabhängigem
mikrobiologischem Test (ISO 20227:2017)
This European Standard was approved by CEN on 9 May 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20227:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 20227:2017) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2018 and conflicting national standards shall
be withdrawn at the latest by January 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 20227:2017 has been approved by CEN as EN ISO 20227:2017 without any modification.
INTERNATIONAL ISO
STANDARD 20227
First edition
2017-06
Water quality — Determination of
the growth inhibition effects of waste
waters, natural waters and chemicals
on the duckweed Spirodela polyrhiza
— Method using a stock culture
independent microbiotest
Qualité de l’eau — Détermination des effets d’inhibition sur la
croissance de la lentille d’eau Spirodela polyrhiza par les eaux usées,
les eaux naturelles et les produits chimiques — Méthode utilisant un
bioessai miniaturisé indépendant d’une culture mère
Reference number
ISO 20227:2017(E)
©
ISO 2017
ISO 20227:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. [phone]
Fax [phone]
[e-mail]
www.iso.org
ii © ISO 2017 – All rights reserved

ISO 20227:2017(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test organisms . 3
6 Growth medium . 3
6.1 Preparation of stock solutions . 4
6.2 Preparation of the final concentration of modified Steinberg medium . 4
7 Apparatus . 4
8 Reference chemicals . 5
9 Procedure. 5
9.1 Germination of the Spirodela polyrhiza turions . 5
9.2 Tests on effluents (and waste waters) . 5
9.2.1 Addition of concentrated growth medium to the effluent sample . 5
9.2.2 Preparation of the effluent dilutions . 6
9.2.3 Procedure . 6
9.3 Tests on chemical compounds . 7
9.3.1 Range finding test . 7
9.3.2 Definitive test . 8
9.4 Filling of the test plate with the toxicant dilutions . 9
9.4.1 General. 9
9.4.2 Procedure . 9
9.5 Transfer of the germinated turions in the test cups .10
9.6 Photo of the multiwell at the start of the toxicity test .10
9.7 Incubation of the multiwell .10
9.8 Photo of the multiwell at the end of the toxicity test .11
9.9 Measurement of the area of the first fronds .11
10 Data treatment — Calculation of the growth inhibition .11
11 Validity criterion .12
12 Test sensitivity .12
13 Test with reference chemicals .12
14 Test report .15
Annex A (informative) Spirodela polyrhiza stock culturing for turion production .16
Annex B (informative) Sensitivity of the Spirodela polyrhiza microbiotest .17
Annex C (informative) Performance data .19
Bibliography .20
ISO 20227:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-go
...