Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO 14902:2001)

This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya
products.
This trypsin inhibitor activity is indicative of the degree of toasting of these products.
The detection limit of the method is 0,5 mg/g.

Futtermittel - Bestimmung der Trypsin-Inhibitoren-Aktivität in Sojaerzeugnissen (ISO 14902:2001)

Diese Internationale Norm legt ein Verfahren für die Bestimmung der Trypsin-Inhibitoren-Aktivität (TIA) in Sojaerzeugnissen fest.
Diese Trypsin-Inhibitoren-Aktivität ist ein Indikator für den Grad der Röstung dieser Erzeugnisse.
Die Nachweisgrenze des Verfahrens liegt bei 0,5 mg/g.

Aliments des animaux - Dosage de l'activité des inhibiteurs trypsiques des produits de soja (ISO 14902:2001)

Krma - Določevanje aktivnosti tripsin inhibitorja sojinih proizvodov (ISO 14902:2001)

General Information

Status
Published
Publication Date
14-Oct-2001
Current Stage
9093 - Decision to confirm - Review Enquiry
Completion Date
16-May-2007

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SLOVENSKI STANDARD
SIST EN ISO 14902:2002
01-junij-2002
.UPD'RORþHYDQMHDNWLYQRVWLWULSVLQLQKLELWRUMDVRMLQLKSURL]YRGRY ,62


Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO

14902:2001)

Futtermittel - Bestimmung der Trypsin-Inhibitoren-Aktivität in Sojaerzeugnissen (ISO

14902:2001)

Aliments des animaux - Dosage de l'activité des inhibiteurs trypsiques des produits de

soja (ISO 14902:2001)
Ta slovenski standard je istoveten z: EN ISO 14902:2001
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN ISO 14902:2002 en

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 14902:2002
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SIST EN ISO 14902:2002
EUROPEAN STANDARD
EN ISO 14902
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2001
ICS 65.120
English version
Animal feeding stuffs - Determination of trypsin inhibitor activity
of soya products (ISO 14902:2001)

Aliments des animaux - Dosage de l'activité des inhibiteurs Futtermittel - Bestimmung der Trypsin-Inhibitoren-Aktivität

trypsiques des produits de soja (ISO 14902:2001) in Sojaerzeugnissen (ISO 14902:2001)

This European Standard was approved by CEN on 3 October 2001.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official

versions.

CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,

Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels

© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14902:2001 E

worldwide for CEN national Members.
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SIST EN ISO 14902:2002
EN ISO 14902:2001 (E)
Foreword

The text of the International Standard ISO 14902:2001 has been prepared by Technical

Committee ISO/TC 34 "Agricultural food products" in collaboration with Technical Committee

CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of

which is held by NEN.

This European Standard shall be given the status of a national standard, either by publication

of an identical text or by endorsement, at the latest by April 2002, and conflicting national

standards shall be withdrawn at the latest by April 2002.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of

the following countries are bound to implement this European Standard: Austria, Belgium,

Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,

Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United

Kingdom.

NOTE FROM CMC The foreword is susceptible to be amended on reception of the German language

version. The confirmed or amended foreword, and when appropriate, the normative annex ZA for the

references to international publications with their relevant European publications will be circulated with

the German version.
Endorsement notice

The text of the International Standard ISO 14902:2001 was approved by CEN as a European

Standard without any modification.
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SIST EN ISO 14902:2002
INTERNATIONAL ISO
STANDARD 14902
First edition
2001-10-15
Animal feeding stuffs — Determination of
trypsin inhibitor activity of soya products
Aliments des animaux — Dosage de l'activité des inhibiteurs trypsiques
des produits de soja
Reference number
ISO 14902:2001(E)
ISO 2001
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SIST EN ISO 14902:2002
ISO 14902:2001(E)
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© ISO 2001

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ii © ISO 2001 – All rights reserved
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SIST EN ISO 14902:2002
ISO 14902:2001(E)
Contents Page

Foreword.....................................................................................................................................................................iv

1 Scope ..............................................................................................................................................................2

2 Normative reference ......................................................................................................................................2

3 Term and definition .......................................................................................................................................2

4 Principle..........................................................................................................................................................2

5 Reagents and materials ................................................................................................................................2

6 Apparatus .......................................................................................................................................................3

7 Sampling.........................................................................................................................................................4

8 Preparation of test sample............................................................................................................................4

9 Procedure .......................................................................................................................................................4

9.1 Number of determinations ............................................................................................................................4

9.2 Sample extraction..........................................................................................................................................4

9.3 Dilution of sample extract.............................................................................................................................5

9.4 Measurement of trypsin activity of working solution ................................................................................5

9.5 Measurement of trypsin inhibitor activity ...................................................................................................5

10 Calculation......................................................................................................................................................6

10.1 Inhibition percentage of sample extract solutions.....................................................................................6

10.2 Trypsin inhibitor activity...............................................................................................................................7

11 Precision.........................................................................................................................................................7

11.1 Interlaboratory tests ......................................................................................................................................7

11.2 Repeatability...................................................................................................................................................7

11.3 Reproducibility...............................................................................................................................................8

12 Test report ......................................................................................................................................................8

Annex A (normative) Dilution scheme for sample extract......................................................................................9

Annex B (informative) Results of interlaboratory test...........................................................................................11

Bibliography..............................................................................................................................................................12

© ISO 2001 – All rights reserved iii
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SIST EN ISO 14902:2002
ISO 14902:2001(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO

member bodies). The work of preparing International Standards is normally carried out through ISO technical

committees. Each member body interested in a subject for which a technical committee has been established has

the right to be represented on that committee. International organizations, governmental and non-governmental, in

liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical

Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.

Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.

Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of

patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

International Standard ISO 14902 was prepared by Technical Committee ISO/TC 34, Food products,

Subcommittee SC 10, Animal feeding stuffs.

Annex A forms a normative part of this International Standard. Annex B is for information only.

iv © ISO 2001 – All rights reserved
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SIST EN ISO 14902:2002
INTERNATIONAL STANDARD ISO 14902:2001(E)
Animal feeding stuffs — Determination of trypsin inhibitor activity
of soya products
1 Scope

This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya

products.

This trypsin inhibitor activity is indicative of the degree of toasting of these products.

The detection limit of the method is 0,5 mg/g.
2 Normative reference

The following normative document contains provisions which, through reference in this text, constitute provisions of

this International Standard. For dated references, subsequent amendments to, or revisions of, any of these

publications do not apply. However, parties to agreements based on this International Standard are encouraged to

investigate the possibility of applying the most recent edition of the normative document indicated below. For

undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC

maintain registers of currently valid International Standards.

ISO 3696:1987, Water for analytical laboratory use — Specification and test methods

3 Term and definition

For the purposes of this International Standard, the following term and definition applies.

3.1
trypsin inhibitor activity
TIA

mass of trypsin inhibited by the procedure described in this International Standard, divided by the mass of the test

sample
NOTE The trypsin inhibitor activity is expressed in milligrams per gram.
4Principle
Trypsin inhibitors are extracted from the sample at pH 9,5.

The remaining trypsin activity is measured by adding benzoyl-L-arginine-p-nitroanilide (L-BAPA) as substrate. The

quantity of released p-nitroaniline is measured spectrometrically.
© ISO 2001 – All rights reserved 1
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SIST EN ISO 14902:2002
ISO 14902:2001(E)
5 Reagents and materials
Use only reagents of recognized analytical grade.
5.1 Water, complying with at least grade 3 in accordance with ISO 3696.
5.2 Sodium hydroxide solution, c(NaOH) = 0,01 mol/l.
5.3 Hydrochloric acid, c(HCl) = 6 mol/l.
5.4 Hydrochloric acid, c(HCl) = 1 mol/l.
5.5 Hydrochloric acid, c(HCl) = 0,1 m
...

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