Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algae

This European standard specifies a laboratory test method for determining the biocidal/biostatic efficacy of film preservatives in a coating against algal growth. The standard does not apply to coatings not susceptible to algal growth. The test method comprises only film preservation, not the protection of the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for wood and masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this standard.

Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von Filmkonservierungsmitteln in einem Beschichtungsstoff gegen Algen

Diese Europäische Norm legt ein Labor-Prüfverfahren zum Bestimmen der bioziden/biostatischen Wirksam¬keit von Filmkonservierungsmitteln in einer Beschichtung gegen Algenbewuchs fest. Die Norm gilt nicht für Beschichtungen, die unempfindlich gegen Algenbewuchs sind. Das Prüfverfahren umfasst nur die Konservie¬rung der Beschichtung, nicht den Schutz des Substrates, z. B. Holz. Dieser wird in einer anderen Norm behandelt. Das Prüfverfahren ist anwendbar für Beschichtungen für Holz und mineralische Substrate. Die Norm gilt nicht für Beschichtungen im maritimen Bereich.
Sicherheit, Gesundheit und Umweltaspekte liegen nicht im Anwendungsbereich dieser Norm.

Peintures et vernis - Méthode d'essai en laboratoire permettant de vérifier l'efficacité des préservateurs du feuil d'un revêtement contre les algues

Barve in laki - Laboratorijske metode za preskušanje učinkovitosti konzervansov filma v premazih proti algam

General Information

Status
Withdrawn
Publication Date
24-Jul-2007
Withdrawal Date
19-Aug-2014
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
20-Aug-2014

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EN 15458:2007
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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against algaePeintures et vernis - Méthode d'essai en laboratoire permettant de vérifier l'efficacité des préservateurs du feuil d'un revetement contre les alguesBeschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von Filmkonservierungsmitteln in einem Beschichtungsstoff gegen AlgenTa slovenski standard je istoveten z:EN 15458:2007SIST EN 15458:2007en,fr,de87.040Barve in lakiPaints and varnishesICS:SLOVENSKI

STANDARDSIST EN 15458:200701-november-2007

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 15458July 2007ICS 87.040 English VersionPaints and varnishes - Laboratory method for testing the efficacyof film preservatives in a coating against algaePeintures et vernis - Méthode d'essai en laboratoirepermettant de vérifier l'efficacité des préservateurs du feuild'un revêtement contre les alguesBeschichtungsstoffe - Laborverfahren für die Prüfung derWirksamkeit von Filmkonservierungsmitteln in einemBeschichtungsstoff gegen AlgenThis European Standard was approved by CEN on 21 June 2007.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36

B-1050 Brussels© 2007 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 15458:2007: E

EN 15458:2007 (E) 2 Contents Page Foreword..............................................................................................................................................................3 Introduction.........................................................................................................................................................4 1 Scope......................................................................................................................................................5 2 Normative references............................................................................................................................5 3 Principle..................................................................................................................................................5 4 Apparatus and materials.......................................................................................................................5 5 Microorganisms.....................................................................................................................................6 6 Sampling and preparation of test samples and of specimens.........................................................6 6.1 Sampling.................................................................................................................................................6 6.2 Preparation of test samples (see Annex A)..........................................................................................6 6.3 Conditioning of the test samples.........................................................................................................6 6.4 Preparation and number of specimens...............................................................................................6 7 Procedure...............................................................................................................................................7 7.1 Preparation of Bold´s Basal Medium ..................................................................................................7 7.2 Preparation of the stock solutions.......................................................................................................7 7.3 Preparation of the Petri dishes with culture medium........................................................................8 7.4 Preparation of stock cultures and sub-cultures.................................................................................8 7.5 Preparation of the algal suspension....................................................................................................8 7.6 Inoculation and incubation (see Annex A)...........................................................................................8 7.7 Assessment............................................................................................................................................9 8 Test report..............................................................................................................................................9 Annex A (informative)

Determination of resistance to algal growth..........................................................10

EN 15458:2007 (E) 3 Foreword This document (EN 15458:2007) has been prepared by Technical Committee CEN/TC 139 “Paints and varnishes”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by January 2008, and conflicting national standards shall be withdrawn at the latest by January 2008. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard : Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EN 15458:2007 (E) 4 Introduction This document identifies criteria to assess the efficacy of film preservatives in a coating against algae. The results of the method allow evaluation of an active ingredient with regard to its inclusion in Annex A of the Biocidal Products Directive 98/8/EC (Directive 98/8/EC of the European Parliament and of the Council of 16 February 1998 concerning the placing of biocidal products on the market – BPD). NOTE The characteristics of the biocide treated coating material should conform to national regulations with regard to health, safety and the environment.

EN 15458:2007 (E) 5 1 Scope This European standard specifies a laboratory test method for determining the biocidal/biostatic efficacy of film preservatives in a coating against algal growth. The standard does not apply to coatings not susceptible to algal growth. The test method comprises only film preservation, not the protection of the substrate itself, e.g. wood, which is dealt with in another standard. The test method is applicable for wood and masonry coatings. It is not applicable to marine coatings. Safety, health and environmental aspects are not in the scope of this standard. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 12469, Biotechnology — Performance criteria for microbiological safety cabinets EN 23270, Paints and varnishes and their raw materials — Temperatures and humidities for conditioning and testing (ISO 3270:1984) EN ISO 1513, Paints and varnishes — Examination and preparation of samples for testing (ISO 1513:1992) 3 Principle For the determination of the algicidal efficacy of film preservatives in a coating, the coating material is applied to a substrate, conditioned according to EN 23270, placed onto an agar surface, inoculated with a standard algal suspension and incubated over a certain period of time under conditions appropriate for algal growth. Conclusions can be drawn with regard to the algicidal efficacy of the film preservatives in a coating from the intensity of the algal growth on the coated surface of the specimen after incubation. The method described in this standard is a semi-quantitative, comparative method between coatings with and without film preservatives. 4 Apparatus and materials 4.1 Cutting device for preparing the specimens (coated filter paper, with a diameter of 55 mm). 4.2 Autoclave for sterilization. 4.3 Incubator, capable of maintaining (23 ± 2) °C. 4.4 Pipette, in the range between 100 µl to 1 000 µl, with sterile tips or combi-tips of 12,5 ml. 4.5 Filter paper without biocidal effect (e.g. cellulose with a pore size of 0,45 µm and a thickness of 650 µm). 4.6 Automatic welding apparatus to seal the bags. 4.7 Sterilized glass bottles (100 ml, 0,5 l, 1 l). 4.8 Sterilized test tubes or other sterilized glassware for preparing the slant agar cultures. 4.9 Bold modified Basal medium as specified in the method (see 7.1). 4.10 Stock solution (see 7.2). 4.11 Culture flask with cap (0,5 l or 1 l). 4.12 Laboratory balance, capable of weighing to an accuracy of 0,1 g. 4.13 Microscope 4.14 Device to determine cell count (commercially available counting chamber, e.g. Thoma chamber). 4.15 Device for application of coating 4.16 Sterile Petri dishes (with a diameter of 94 mm and a height of

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