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EN ISO 14501:2021

(Main)

Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography (ISO 14501:2021)

Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography (ISO 14501:2021)

This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 μg/kg for milk powder and 0,005 μg/kg for liquid milk. The limit of quantification (LOQ) is 0,1 μg/kg for milk powder and 0,01 μg/kg for liquid milk.
The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.

Milch und Milchpulver - Bestimmung des Gehalts an Aflatoxin M1 - Reinigung durch Immunaffinitäts-Chromatographie und Bestimmung mit Hochleistungs-Flüssigchromatographie (ISO 14501:2021)

Dieses Dokument legt ein Verfahren zur Bestimmung des Aflatoxin-M1-Gehalts von Milch und Milchpulver fest. Der kleinste Validierungswert beträgt 0,08 μg/kg für Vollmilchpulver, d. h. 0,008 μg/l für rekonstituierte flüssige Milch. Die Nachweisgrenze (LOD, en: limit of detection) beträgt 0,05 μg/kg für Milchpulver sowie 0,005 μg/kg für flüssige Milch. Die Bestimmungsgrenze (LOQ, en: limit of quantification) beträgt 0,1 μg/kg für Milchpulver sowie 0,01 μg/kg für flüssige Milch.
Das Verfahren ist auch auf fettarme Milch, Magermilch, fettarmes Milchpulver und Magermilchpulver anwendbar.

Lait et lait en poudre - Détermination de la teneur en aflatoxine M1 - Purification par chromatographie d'immunoaffinité et détermination par chromatographie en phase liquide à haute performance (ISO 14501:2021)

Le présent document spécifie une méthode pour le dosage de l’aflatoxine M1 dans le lait et le lait en poudre. Le niveau de validation le plus bas est de 0,08 µg/kg de poudre de lait entier, ce qui correspond à 0,008 µg/l de lait liquide reconstitué. La limite de détection (LOD) est de 0,05 μg/kg pour le lait en poudre et de 0,005 μg/kg pour le lait liquide. La limite de quantification (LOQ) est de 0,1 μg/kg pour le lait en poudre et de 0,01 μg/kg pour le lait liquide.
La méthode est également applicable au lait à faible teneur en matière grasse, au lait écrémé et au lait en poudre à faible teneur en matière grasse ou écrémé.

Mleko in mleko v prahu - Določevanje vsebnosti aflatoksina M1 - Čiščenje z imunoafinitetno kromatografijo in določevanje s tekočinsko kromatografijo visoke ločljivosti (ISO 14501:2021)

General Information

Status
Published
Publication Date
25-May-2021
Withdrawal Date
29-Nov-2021
ICS
67.100.10 - Milk and processed milk products
Technical Committee
CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
Drafting Committee
CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
26-May-2021
Completion Date
26-May-2021
Ref Project
SIST EN ISO 14501:2021 - Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography (ISO 14501:2021)

Relations

Replaces
EN ISO 14501:2007 - Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography (ISO 14501:2007)
Effective Date
02-Jun-2021

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SLOVENSKI STANDARD
SIST EN ISO 14501:2021
01-julij-2021
Nadomešča:
SIST EN ISO 14501:2007
Mleko in mleko v prahu - Določevanje vsebnosti aflatoksina M1 - Čiščenje z
imunoafinitetno kromatografijo in določevanje s tekočinsko kromatografijo visoke
ločljivosti (ISO 14501:2021)
Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by
immunoaffinity chromatography and determination by high-performance liquid
chromatography (ISO 14501:2021)
Milch und Milchpulver - Bestimmung des Gehalts an Aflatoxin M1 - Reinigung durch
Immunaffinitäts-Chromatographie und Bestimmung mit Hochleistungs-
Flüssigchromatographie (ISO 14501:2021)
Lait et lait en poudre - Détermination de la teneur en aflatoxine M1 - Purification par
chromatographie d'immunoaffinité et détermination par chromatographie en phase
liquide à haute performance (ISO 14501:2021)
Ta slovenski standard je istoveten z: EN ISO 14501:2021
ICS:
67.100.10 Mleko in predelani mlečni Milk and processed milk
proizvodi products
SIST EN ISO 14501:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 14501:2021

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SIST EN ISO 14501:2021


EN ISO 14501
EUROPEAN STANDARD

NORME EUROPÉENNE

May 2021
EUROPÄISCHE NORM
ICS 67.100.10 Supersedes EN ISO 14501:2007
English Version

Milk and milk powder - Determination of aflatoxin M1
content - Clean-up by immunoaffinity chromatography and
determination by high-performance liquid
chromatography (ISO 14501:2021)
Lait et lait en poudre - Détermination de la teneur en Milch und Milchpulver - Bestimmung des Gehalts an
aflatoxine M1 - Purification par chromatographie Aflatoxin M1 - Reinigung durch Immunaffinitäts-
d'immunoaffinité et détermination par Chromatographie und Bestimmung mit Hochleistungs-
chromatographie en phase liquide à haute Flüssigchromatographie (ISO 14501:2021)
performance (ISO 14501:2021)
This European Standard was approved by CEN on 12 April 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14501:2021 E
worldwide for CEN national Members.

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SIST EN ISO 14501:2021
EN ISO 14501:2021 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 14501:2021
EN ISO 14501:2021 (E)
European foreword
This document (EN ISO 14501:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2021, and conflicting national standards
shall be withdrawn at the latest by November 2021.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 14501:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
...

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