EN 16868:2019

Ambient air - Sampling and analysis of airborne pollen grains and fungal spores for networks related to allergy - Volumetric Hirst method

This document specifies the procedure to sample continuously and to analyse the concentration of airborne pollen grains and fungal spores in ambient air using the volumetric Hirst type sampler [1] [2] [3] (see Annex A) or an even equivalent method assuring comparable data.
This document describes both the sampling and the analysis procedures for the purpose of networks related to allergy. For the other tasks mentioned in the introduction, other specifications may be required.

Außenluft - Probenahme und Analyse luftgetragener Pollen und Pilzsporen für Allergienetzwerke - Volumetrische Hirst-Methode

Dieses Dokument legt das Verfahren fest zur kontinuierlichen Probenahme und zur Analyse der Konzentration luftgetragener Pollenkörner und Pilzsporen in der Umgebungsluft mittels eines volumetrischen Sammlers nach dem Hirst-Prinzip [1] [2] [3] (siehe Anhang A) oder einer gleichwertigen, vergleichbare Daten liefernden Methode.
Dieses Dokument beschreibt sowohl die Probenahme als auch die Analyseverfahren für die Zwecke von Allergienetzwerken. Für die anderen in der Einleitung erwähnten Aufgaben können andere Spezifikationen erforderlich sein.

Air ambiant - Échantillonnage et analyse des grains de pollen en suspension dans l'air et des spores fongiques pour les réseaux relatifs à l’allergie - Méthode volumétrique de Hirst

La présente Norme européenne spécifie le mode opératoire pour l’échantillonnage en continu et l’analyse de la concentration en grains de pollen et spores fongiques dans l’air ambiant en utilisant l’échantillonneur volumétrique de Hirst [1] [2] [3] (voir l’Annexe A) ou toute autre méthode équivalente garantissant des données comparables.
La présente Norme européenne décrit les modes opératoires d’échantillonnage et d’analyse aux fins des réseaux relatifs à l’allergie. Pour les autres objectifs mentionnés dans l’introduction, des spécifications différentes peuvent être requises.

Zunanji zrak - Vzorčenje in analiza cvetnega prahu in trosov gliv v zraku za alergijsko omrežje - Volumetrična Hirstova metoda

Ta dokument določa postopek za stalno vzorčenje in analiziranje koncentracije cvetnega prahu ter trosov gliv v zunanjem zraku z volumetričnim vzorčevalnikom tipa Hirst. Ta dokument opisuje postopke vzorčenja in tudi analize za namen alergijskih omrežij. Morda so potrebne druge specifikacije. Med izpostavljenostjo pelodnim zrnom in trosom gliv ter onesnaženostjo zraka obstaja sinergija, za katero so potrebne nekatere določene in dodatne informacije, da se lahko zmanjša tveganje za vpliv na zdravje.

General Information

• Status: Published
• Publication Date: 07-May-2019
• Withdrawal Date: 29-Nov-2019
• ICS: 13.040.20 - Ambient atmospheres
• Technical Committee: CEN/TC 264 - Air quality
• Drafting Committee: CEN/TC 264/WG 39 - Sampling and analysis of airborne pollen grains and fungal spores
• Current Stage: 9020 - Submission to 2 Year Review Enquiry - Review Enquiry
• Start Date: 15-Jul-2024
• Completion Date: 15-Jul-2024
• Ref Project: SIST EN 16868:2019 - Ambient air - Sampling and analysis of airbone pollen grains and fungal spores for networks related to allergy - Volumetric Hirst method

Relations

• Replaces: CEN/TS 16868:2015 - Ambient air - Sampling and analysis of airborne pollen grains and fungal spores for allergy networks - Volumetric Hirst method
• Effective Date: 15-May-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2019
Nadomešča:
SIST-TS CEN/TS 16868:2016
Zunanji zrak - Vzorčenje in analiza cvetnega prahu in trosov gliv v zraku za
alergijsko omrežje - Volumetrična Hirstova metoda
Ambient air - Sampling and analysis of airbone pollen grains and fungal spores for
networks related to allergy - Volumetric Hirst method
Außenluft - Probenahme und Analyse luftgetragener Pollen und Pilzsporen für
Allergienetzwerke - Volumetrische Hirst-Methode
Air ambiant - Échantillonnage et analyse des grains de pollen et des spores fongiques
aériens pour les réseaux relatifs à l'allergie - Méthode volumétrique de Hirst
Ta slovenski standard je istoveten z: EN 16868:2019
ICS:
13.040.20 Kakovost okoljskega zraka Ambient atmospheres
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 16868
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2019
EUROPÄISCHE NORM
ICS 13.040.20 Supersedes CEN/TS 16868:2015
English Version
Ambient air - Sampling and analysis of airborne pollen
grains and fungal spores for networks related to allergy -
Volumetric Hirst method
Air ambiant - Échantillonnage et analyse des grains de Außenluft - Probenahme und Analyse luftgetragener
pollen en suspension dans l'air et des spores fongiques Pollen und Pilzsporen für Allergienetzwerke -
pour les réseaux relatifs à l'allergie - Méthode Volumetrische Hirst-Methode
volumétrique de Hirst
This European Standard was corrected and reissued by the CEN-CENELEC Management Centre on 12 June 2019.

This European Standard was approved by CEN on 8 March 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, North
Macedonia, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

E UR O P ÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 16868:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 10
5 Sampling . 10
5.1 Equipment . 10
5.1.1 Apparatus . 10
5.1.2 Sampling support . 14
5.1.3 Installation conditions . 16
5.2 Operating procedure . 16
5.2.1 Preparation of the coating medium . 16
5.2.2 Support preparation . 17
5.2.3 Changing of the drum . 18
6 Analysis . 18
6.1 Equipment . 18
6.2 Operating procedure . 19
6.2.1 Support . 19
6.2.2 Mounting medium . 19
6.3 Methodology for counting . 19
6.3.1 Glass slide preparation for microscopy analysis for drum tape . 19
6.3.2 Optical microscopy . 21
6.3.3 Identification . 22
6.3.4 Counting method . 22
6.3.5 Data recording . 22
6.3.6 Conversion factor . 23
7 Performance characteristics for pollen and fungal spores counts . 24
7.1 General . 24
7.2 Integrated uncertainty assessment . 24
7.3 Uncertainty from counting error and counting routine. 24
7.4 Measurement uncertainty relating to sampling efficiency . 24
7.5 Measurement uncertainty relating to capture film, adhesive and specimen
preparation . 24
7.6 Measurement uncertainty relating to time discrimination . 25
7.7 Measurement uncertainty related to the detection limit . 25
7.8 Measurement uncertainty in relation to the calibration of the flow rate . 25
7.9 Measurement uncertainty relating to spatial representativity . 25
8 Quality assurance . 25
8.1 General . 25
8.2 Measurement site/trap . 25
8.2.1 Control . 25
8.2.2 Characterization of the site and its ambient conditions (passport of sampling site) . 25
8.2.3 Spatial representativity . 26
8.3 Analyst . 26
8.4 Intra- and interlaboratory quality assessments . 26
8.4.1 General . 26
8.4.2 Repeatability . 26
8.4.3 Reproducibility and accuracy . 26
8.4.4 Sensitivity and specificity . 27
8.5 Network monitoring management . 27
Annex A (informative) Hirst type volumetric trap . 28
Annex B (informative) Pictures of impaction support . 29
Annex C (informative) Material Safety Data Sheets . 31
Annex D (informative) Identification key . 32
Bibliography . 37

European foreword
This document (EN 16868:2019) has been prepared by Technical Committee CEN/TC 264 “Air quality”,
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2019, and conflicting national standards shall
be withdrawn at the latest by November 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 16868:2015.
The main changes with respect to the previous edition are listed below:
a) the title has been changed;
b) modifications have been made to the Introduction, the Scope and Clauses 3, 4, 5 and 6;
c) new paragraphs have been added to Clauses 7 and 8;
d) modifications have been made to all Annexes;
e) Figures D.2 and D.3 have been modified;
f) the Bibliography has been readjusted;
g) editorial changes have been made.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands,
Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
the United Kingdom.
Introduction
Biological particles (pollen and fungal spores) are present in the air, causing health impacts at various
levels. In Europe, a lot of people suffer from pollinosis due to pollen and/or fungal spores (EFA, European
Federation of Allergy and Airways Diseases Patients Association, 2017). Pollen grains and fungal spores
are considered in some Member States as an air pollutant as well as particles suspended in the air
(PM ). In Europe, European Aerobiology Society (EAS) in coordination with International
10,2,5
Association for Aerobiology (IAA) manage the methodology of sampling, analysis, quality control,
development and information.
Persons and institutions involved in pollen forecasting have a scientific and public health responsibility.
A pollen forecast is a guideline for allergen avoidance with a direct influence on pollen allergy sufferers
and their behaviour. Pollen allergy sufferers are in need of such information since pollen allergy affects
their quality of life and pollen and spores are an abundant, environmental aller
...