EN 13946:2014
(Main)Water quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes
Water quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes
This European Standard specifies a method for the sampling and laboratory preparation of benthic diatoms for ecological status and water quality assessments. Data produced by this method are suitable for production of water quality indices based on the relative abundance of taxa.
Wasserbeschaffenheit - Anleitung zur Probenahme und Probenaufbereitung von benthischen Kieselalgen aus Fließgewässern und Seen
Diese Europäische Norm legt ein Verfahren zur Probenahme und Laborpräparation benthischer Diatomeen zur Überwachung des ökologischen Zustands und der Wasserbeschaffenheit fest. Daten, die nach dieser Methode erhoben werden, sind zur Erzeugung von Wasserbeschaffenheitsindizes, die auf der relativen Häufigkeit der Taxa basieren, geeignet.
Qualité de l'eau - Guide pour l'échantillonnage en routine et le prétraitement des diatomées benthiques de rivières et de plans d'eau
La présente Norme européenne spécifie une méthode d’échantillonnage et de préparation en laboratoire de diatomées benthiques en vue d’évaluer l’état écologique et la qualité de l’eau. Les données obtenues par cette méthode conviennent pour la production d'indices de qualité de l'eau basés sur l’abondance relative des taxons.
Kakovost vode - Navodilo za rutinsko vzorčenje in pripravo vzorcev bentoških kremenastih alg rek in jezer
Standard EN 13946 določa metodo za vzorčenje in laboratorijsko pripravo bentoških kremenastih alg za ocene ekološkega stanja in kakovosti vode. Podatki, pridobljeni s to metodo, so primerni za pripravo indeksov kakovosti vode, ki temeljijo na relativni številčnosti tega reda.
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wasserbeschaffenheit - Anleitung zur Probenahme und Probenaufbereitung von benthischen Kieselalgen aus Fließgewässern und SeenQualité de l'eau - Guide pour l'échantillonnage en routine et le prétraitement des diatomées benthiques de rivières et de plans d'eauWater quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of water13.060.10Voda iz naravnih virovWater of natural resourcesICS:Ta slovenski standard je istoveten z:EN 13946:2014SIST EN 13946:2014en,fr,de01-maj-2014SIST EN 13946:2014SLOVENSKI
STANDARDSIST EN 13946:20031DGRPHãþD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 13946
March 2014 ICS 13.060.70 Supersedes EN 13946:2003English Version
Water quality - Guidance for the routine sampling and preparation of benthic diatoms from rivers and lakes
Qualité de l'eau - Guide pour l'échantillonnage en routine et le prétraitement des diatomées benthiques de rivières et de plans d'eau
Wasserbeschaffenheit - Anleitung zur Probenahme und Probenaufbereitung von benthischen Kieselalgen aus Fließgewässern und Seen This European Standard was approved by CEN on 20 December 2013.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 13946:2014 ESIST EN 13946:2014
Foreword .3 Introduction .4 1 Scope .5 2 Terms and definitions .5 3 Principle .6 4 Equipment .6 4.1 Equipment for field sampling .6 4.2 Laboratory equipment .6 5 Reagents .6 6 Procedure .8 6.1 Choice of substratum .8 6.2 Sample site selection .8 6.3 Sampling methods .8 6.3.1 Moveable natural hard surfaces .8 6.3.2 Method for sampling vertical man-made surfaces in situ .9 6.3.3 Use of artificial substrata . 10 6.3.4 Sample collection from submerged macrophytes and macroalgae . 10 6.3.5 Sample collection from emergent macrophytes . 10 6.4 Preparation prior to microscopic examination . 11 6.4.1 Preservation and preliminary laboratory treatment . 11 6.4.2 Methods for cleaning diatoms . 11 6.4.3 Preparation of permanent slides . 11 Annex A (informative)
Methods for cleaning diatoms for microscopic examination . 13 A.1 General . 13 A.2 Method 1: Hot hydrogen peroxide . 13 A.2.1 Apparatus . 13 A.2.2 Reagents . 13 A.2.3 Procedure . 13 A.3 Method 2: Cold hydrogen peroxide . 14 A.3.1 Apparatus and reagents . 14 A.3.2 Procedure . 14 A.4 Method 3: Hot hydrogen peroxide with potassium dichromate . 14 A.4.1 Apparatus . 14 A.4.2 Reagents . 14 A.4.3 Procedure . 14 A.5 Method 4: Cold acid (permanganate) method of cleaning . 15 A.5.1 Apparatus . 15 A.5.2 Reagents . 15 A.5.3 Procedure . 15 Bibliography . 17
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