CEN/TS 13130-13:2005
(Main)Materials and articles in contact with foodstuffs - Plastics substances subject to limitation - Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Materials and articles in contact with foodstuffs - Plastics substances subject to limitation - Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty food simulants, sunflower oil and a mixture of synthetic triglycerides.
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen, die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
Diese Technische Spezifikation, Teil der EN 13130, legt ein Verfahren zur Bestimmung von Bisphenol A in den Prüflebensmitteln Wasser, 3%ige Essigsäure (m/V) in wässriger Lösung, 15%iges Ethanol (Volumenanteil) in wässriger Lösung und rektifiziertes Olivenöl fest. Der ermittelte Gehalt an monomerem Bisphenol A wird in Milligramm Bisphenol A je Kilogramm Prüflebensmittel angegeben. Das Verfahren eignet sich zur quantitativen Bestimmung von Bisphenol A bei einem Mindestgehalt von 0,2 mg je Kilogramm Prüflebensmittel bis 0,7 mg je Kilogramm Prüflebensmittel.
ANMERKUNG Das Verfahren sollte auch auf andere wässrige Prüflebensmittel sowie auf die anderen fettigen Prüflebensmittel, Sonnenblumenöl und eine Mischung synthetischer Triglyceride anwendbar sein.
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les matières plastiques soumises à des limitations - Partie 13 : Détermination du 2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les simulants d'aliments
Materiali in predmeti v stiku z živili - Snovi v polimernih materialih, katerih koncentracija je omejena - 13. del: Določevanje 2,2-bis(4-hidroksifenil)propana (Bisfenola A) v modelnih raztopinah za živila
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2005
0DWHULDOLLQSUHGPHWLYVWLNX]åLYLOL6QRYLYSROLPHUQLKPDWHULDOLKNDWHULK
NRQFHQWUDFLMDMHRPHMHQDGHO 'RORþHYDQMHELV KLGURNVLIHQLO SURSDQD
%LVIHQROD$ YPRGHOQLKUD]WRSLQDK]DåLYLOD
Materials and articles in contact with foodstuffs - Plastics substances subject to limitation
- Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food
simulants
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen,
die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-Hydroxyphenyl)
Propan (Bisphenol A) in Prüflebensmitteln
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les
matieres plastiques soumises a des limitations - Partie 13 : Détermination du 2,2-bis(4-
hydroxyphenyl) propane (Bisphénol A) dans les simulants d'aliments
Ta slovenski standard je istoveten z: CEN/TS 13130-13:2005
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN/TS 13130-13
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2005
ICS 67.250
English version
Materials and articles in contact with foodstuffs - Plastics
substances subject to limitation - Part 13: Determination of 2,2-
bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Matériaux et objets en contact avec les denrées Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln
alimentaires - Substances dans les matières plastiques - Substanzen in Kunststoffen, die Beschränkungen
soumises à des limitations - Partie 13 : Détermination du unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
simulants d'aliments
This Technical Specification (CEN/TS) was approved by CEN on 16 December 2004 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 13130-13:2005: E
worldwide for CEN national Members.
Contents
page
Foreword. 3
Introduction . 5
1 Scope. 6
2 Normative references . 6
3 Principle . 6
4 Reagents . 7
5 Apparatus . 7
6 Samples . 8
7 Procedure . 9
8 Expression of results. 10
9 Confirmation. 11
10 Test report . 12
Bibliography . 13
Foreword
This document (CEN/TS 13130-13:2005) has been prepared by Technical Committee CEN/TC 194
“Utensils in contact with food”, the secretariat of which is held by BSI.
This part of EN 13130 has been prepared within the Standards, Measurement and Testing project,
MAT1-CT92-0006, “Development of Methods of Analysis for Monomers” and has been prepared by
Subcommittee (SC 1) of TC 194 "Utensils in contact with food" as one of a series of test methods for
plastics materials and articles in contact with foodstuffs.
This standard is intended to support Directives 2002/72/EC [1], 89/109/EEC [2], 82/711/EEC [3] and
its amendments 93/8/EEC [4] and 97/48/EC [5], and 85/572/EEC [6].
At the time of preparation and publication of this part of EN 13130 the European Union legislation
relating to plastics materials and articles intended to come into contact with foodstuffs is incomplete.
Further Directives and amendments to existing Directives are expected which could change the
legislative requirements which this standard supports. It is therefore strongly recommended that users
of this standard refer to the latest relevant published Directive(s) before commencement of a test or
tests described in this standard.
This part of EN 13130 should be read in conjunction with EN 13130-1.
Further parts of EN 13130, under the general title Materials and articles in contact with foodstuffs -
Plastics substances subject to limitation, have been prepared, and others are in preparation,
concerned with the determination of specific migration from plastics materials into foodstuffs and food
simulants and the determination of specific monomers and additives in plastics. The parts of
EN 13130 are as follows.
Part 1: Guide to test methods for the specific migration of substances from plastics to foods and food
simulants and the determination of substances in plastics and the selection of conditions of exposure
to food simulants
Part 2: Determination of terephthalic acid in food simulants
Part 3: Determination of acrylonitrile in food and food simulants
Part 4: Determination of 1,3-butadiene in plastics
Part 5: Determination of vinylidene chloride in food simulants
Part 6: Determination of vinylidene chloride in plastics
Part 7: Determination of monoethylene glycol and diethylene glycol in food simulants
Part 8: Determination of isocyanates in plastics
Part 9: Determination of acetic acid, vinyl ester in food simulants
Part 10: Determination of acrylamide in food simulants
Part 11: Determination of 11-aminoundecanoic acid in food simulants
Part 12: Determination of 1,3-benzenedimethanamine in food simulants
Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Part 14: Determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indoline in food simulants
Part 15: Determination of 1,3-butadiene in food simulants
Part 16: Determination of caprolactam and caprolactam salt in food simulants
Part 17: Determination of carbonyl chloride in plastics
Part 18: Determination of 1,2-dihydroxybenzene, 1,3-dihydroxybenzene,
1,4-dihydroxybenzene, 4,4’-dihydroxybenzophenone and 4,4’dihydroxybiphenyl in food simulants
Part 19: Determination of dimethylaminoethanol in food simulants
Part 20: Determination of epichlorohydrin in plastics
Part 21: Determination of ethylenediamine and hexamethylenediamine in food simulants
Part 22: Determination of ethylene oxide and propylene oxide in plastics
Part 23: Determination of formaldehyde and hexamethylenetetramine in food simulants
Part 24: Determination of maleic acid and maleic anhydride in food simulants
Part 25: Determination of 4-methyl-pentene in food simulants
Part 26: Determination of 1-octene and tetrahydrofuran in food simulants
Part 27: Determination of 2,4,6-triamino-1,3,5-triazine in food simulants
Part 28: Determination of 1,1,1-trimethylolpropane in food simulants
Parts 1 to 8 are European Standards. Parts 9 to 28 are Technical Specifications.
WARNING All chemicals are hazardous to health to a greater or lesser extent. It is beyond
the scope of this Technical Specification to give instructions for the safe handling of all
chemicals, that meet, in full, the legal obligations in all countries in which this Technical
Specification may be followed. Therefore, specific warnings are not given and users of this
Technical Specification should ensure that they meet all the necessary safety requirements in
their own country.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this CEN Technical Specification: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Introduction
2,2-bis(4-hydroxyphenyl)propane, hereinafter referred to as Bisphenol A, C H O , PM/Ref. 13480, is
15 16 2
a monomer used in the manufacture of certain plastics materials and articles intended to come into
contact with foodstuffs. After manufacture residual Bisphenol A can remain in the finished product and
may migrate into foodstuffs coming into contact with that product.
The method has been pre-validated by a collaborative trial with three laboratories.
1 Scope
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food
simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive
oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per
kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A
at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty
food simulants, sunflower oil and a mixture of synthetic triglycerides.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 13130-1:2004, Materials and articles in contact with foodstuffs – Plastics substances subject to
limitation – Part 1: Guide to test methods for the specific migration of substances from plastics to
foods and food simulants and the determination of substances in plastics and the selection of
conditions of exposure to food simulants.
3 Principle
The level of Bisphenol A in aqueous food simulants is determined by high performance liquid
chromatography (HPLC) with ultra violet (UV) detection. Olive oil test samples are extracted with a
mixture of water/methanol and the resultant solution analyzed by HPLC. Calibration is achieved by
analysis of relevant simulants containing known amounts of Bisphenol A. Confirmation of Bisphenol A
is carried out by diode array detection.
4 Reagents
NOTE All reagents should be of recognized analytical quality unless otherwise stated.
4.1 Analyte
2,2-bis(4-hydroxyphenyl)propane (Bisphenol A or 4,4'-(methylethylidene)-bisphenol or
4,4'-isopropylidenediphenol), C H O , molecular weight: 228,28, purity > 99 %.
15 16 2
4.2 Chemicals
4.2.1 n-Hexane
4.2.2 Methanol
4.2.3 Water, deionized
4.3 Solutions
4.3.1 Extraction solvent, methanol/water = 1:1
Measure 100 ml of methanol (4.2.2) and 100 ml of water (4.2.3) and mix.
4.3.2 Mobile phase for HPLC, methanol/water = 70:30
Measure 500 ml of methanol (4.2.2) and 215 ml of water (4.2.3) and mix.
4.3.3 Stock solution of Bisphenol A in methanol at a defined concentration of approximately
0,38 mg/ml
Weigh to the nearest 0,1 mg approximately 37,5 mg of Bisphenol A (4.1.1) into a 100 ml volumetric
flask. Dissolve the Bisphenol A in methanol and make up to the mark with methanol (4.2.2).
Calculate the concentration in micrograms of Bisphenol A per millilitre of solution.
Repeat the procedure to obtain a second stock solution.
NOTE The solution can be stored in a well closed container in dark for a maximum of 3 months at any
temperature between +20 °C and –20 °C.
5 Apparatus
NOTE An instrument or item of apparatus is listed only where it is special or made to a particular
specification, usual laboratory glassware and equipment being assumed to be available.
5.1 High performance liquid chromatograph, preferably, equipped with an automatic 20 µl loop
injector and a variable wavelength UV detector conn
...
SLOVENSKI STANDARD
01-april-2005
Materiali in predmeti v stiku z živili - Snovi v polimernih materialih, katerih
koncentracija je omejena - 13. del: Določevanje 2,2-bis(4-hidroksifenil)propana
(Bisfenola A) v modelnih raztopinah za živila
Materials and articles in contact with foodstuffs - Plastics substances subject to limitation
- Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food
simulants
Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Substanzen in Kunststoffen,
die Beschränkungen unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
Matériaux et objets en contact avec les denrées alimentaires - Substances dans les
matieres plastiques soumises a des limitations - Partie 13 : Détermination du 2,2-bis(4-
hydroxyphenyl) propane (Bisphénol A) dans les simulants d'aliments
Ta slovenski standard je istoveten z: CEN/TS 13130-13:2005
ICS:
67.250 Materiali in predmeti v stiku z Materials and articles in
živili contact with foodstuffs
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN/TS 13130-13
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
February 2005
ICS 67.250
English version
Materials and articles in contact with foodstuffs - Plastics
substances subject to limitation - Part 13: Determination of 2,2-
bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Matériaux et objets en contact avec les denrées Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln
alimentaires - Substances dans les matières plastiques - Substanzen in Kunststoffen, die Beschränkungen
soumises à des limitations - Partie 13 : Détermination du unterliegen - Teil 13: Bestimmung von 2,2-Bis(4-
2,2-bis(4-hydroxyphènyl) propane (Bisphénol A) dans les Hydroxyphenyl)Propan (Bisphenol A) in Prüflebensmitteln
simulants d'aliments
This Technical Specification (CEN/TS) was approved by CEN on 16 December 2004 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 13130-13:2005: E
worldwide for CEN national Members.
Contents
page
Foreword. 3
Introduction . 5
1 Scope. 6
2 Normative references . 6
3 Principle . 6
4 Reagents . 7
5 Apparatus . 7
6 Samples . 8
7 Procedure . 9
8 Expression of results. 10
9 Confirmation. 11
10 Test report . 12
Bibliography . 13
Foreword
This document (CEN/TS 13130-13:2005) has been prepared by Technical Committee CEN/TC 194
“Utensils in contact with food”, the secretariat of which is held by BSI.
This part of EN 13130 has been prepared within the Standards, Measurement and Testing project,
MAT1-CT92-0006, “Development of Methods of Analysis for Monomers” and has been prepared by
Subcommittee (SC 1) of TC 194 "Utensils in contact with food" as one of a series of test methods for
plastics materials and articles in contact with foodstuffs.
This standard is intended to support Directives 2002/72/EC [1], 89/109/EEC [2], 82/711/EEC [3] and
its amendments 93/8/EEC [4] and 97/48/EC [5], and 85/572/EEC [6].
At the time of preparation and publication of this part of EN 13130 the European Union legislation
relating to plastics materials and articles intended to come into contact with foodstuffs is incomplete.
Further Directives and amendments to existing Directives are expected which could change the
legislative requirements which this standard supports. It is therefore strongly recommended that users
of this standard refer to the latest relevant published Directive(s) before commencement of a test or
tests described in this standard.
This part of EN 13130 should be read in conjunction with EN 13130-1.
Further parts of EN 13130, under the general title Materials and articles in contact with foodstuffs -
Plastics substances subject to limitation, have been prepared, and others are in preparation,
concerned with the determination of specific migration from plastics materials into foodstuffs and food
simulants and the determination of specific monomers and additives in plastics. The parts of
EN 13130 are as follows.
Part 1: Guide to test methods for the specific migration of substances from plastics to foods and food
simulants and the determination of substances in plastics and the selection of conditions of exposure
to food simulants
Part 2: Determination of terephthalic acid in food simulants
Part 3: Determination of acrylonitrile in food and food simulants
Part 4: Determination of 1,3-butadiene in plastics
Part 5: Determination of vinylidene chloride in food simulants
Part 6: Determination of vinylidene chloride in plastics
Part 7: Determination of monoethylene glycol and diethylene glycol in food simulants
Part 8: Determination of isocyanates in plastics
Part 9: Determination of acetic acid, vinyl ester in food simulants
Part 10: Determination of acrylamide in food simulants
Part 11: Determination of 11-aminoundecanoic acid in food simulants
Part 12: Determination of 1,3-benzenedimethanamine in food simulants
Part 13: Determination of 2,2-bis(4-hydroxyphenyl)propane (Bisphenol A) in food simulants
Part 14: Determination of 3,3-bis(3-methyl-4-hydroxyphenyl)-2-indoline in food simulants
Part 15: Determination of 1,3-butadiene in food simulants
Part 16: Determination of caprolactam and caprolactam salt in food simulants
Part 17: Determination of carbonyl chloride in plastics
Part 18: Determination of 1,2-dihydroxybenzene, 1,3-dihydroxybenzene,
1,4-dihydroxybenzene, 4,4’-dihydroxybenzophenone and 4,4’dihydroxybiphenyl in food simulants
Part 19: Determination of dimethylaminoethanol in food simulants
Part 20: Determination of epichlorohydrin in plastics
Part 21: Determination of ethylenediamine and hexamethylenediamine in food simulants
Part 22: Determination of ethylene oxide and propylene oxide in plastics
Part 23: Determination of formaldehyde and hexamethylenetetramine in food simulants
Part 24: Determination of maleic acid and maleic anhydride in food simulants
Part 25: Determination of 4-methyl-pentene in food simulants
Part 26: Determination of 1-octene and tetrahydrofuran in food simulants
Part 27: Determination of 2,4,6-triamino-1,3,5-triazine in food simulants
Part 28: Determination of 1,1,1-trimethylolpropane in food simulants
Parts 1 to 8 are European Standards. Parts 9 to 28 are Technical Specifications.
WARNING All chemicals are hazardous to health to a greater or lesser extent. It is beyond
the scope of this Technical Specification to give instructions for the safe handling of all
chemicals, that meet, in full, the legal obligations in all countries in which this Technical
Specification may be followed. Therefore, specific warnings are not given and users of this
Technical Specification should ensure that they meet all the necessary safety requirements in
their own country.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to announce this CEN Technical Specification: Austria, Belgium, Cyprus,
Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Introduction
2,2-bis(4-hydroxyphenyl)propane, hereinafter referred to as Bisphenol A, C H O , PM/Ref. 13480, is
15 16 2
a monomer used in the manufacture of certain plastics materials and articles intended to come into
contact with foodstuffs. After manufacture residual Bisphenol A can remain in the finished product and
may migrate into foodstuffs coming into contact with that product.
The method has been pre-validated by a collaborative trial with three laboratories.
1 Scope
This document, part of EN 13130, specifies a method for the determination of Bisphenol A in the food
simulants water, 3 % w/v acetic acid aqueous, 15 % v/v ethanol aqueous solution and rectified olive
oil. The level of Bisphenol A monomer determined is expressed as milligrams Bisphenol A per
kilogram of food simulant. The method is applicable to the quantitative determination of Bisphenol A
at a minimum level of 0,2 mg/kg to 0,7 mg per kilogram of food simulants.
NOTE The method should also be applicable to other aqueous food simulants as well as to the other fatty
food simulants, sunflower oil and a mixture of synthetic triglycerides.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 13130-1:2004, Materials and articles in contact with foodstuffs – Plastics substances subject to
limitation – Part 1: Guide to test methods for the specific migration of substances from plastics to
foods and food simulants and the determination of substances in plastics and the selection of
conditions of exposure to food simulants.
3 Principle
The level of Bisphenol A in aqueous food simulants is determined by high performance liquid
chromatography (HPLC) with ultra violet (UV) detection. Olive oil test samples are extracted with a
mixture of water/methanol and the resultant solution analyzed by HPLC. Calibration is achieved by
analysis of relevant simulants containing known amounts of Bisphenol A. Confirmation of Bisphenol A
is carried out by diode array detection.
4 Reagents
NOTE All reagents should be of recognized analytical quality unless otherwise stated.
4.1 Analyte
2,2-bis(4-hydroxyphenyl)propane (Bisphenol A or 4,4'-(methylethylidene)-bisphenol or
4,4'-isopropylidenediphenol), C H O , molecular weight: 228,28, purity > 99 %.
15 16 2
4.2 Chemicals
4.2.1 n-Hexane
4.2.2 Methanol
4.2.3 Water, deionized
4.3 Solutions
4.3.1 Extraction solvent, methanol/water = 1:1
Measure 100 ml of methanol (4.2.2) and 100 ml of water (4.2.3) and mix.
4.3.2 Mobile phase for HPLC, methanol/water = 70:30
Measure 500 ml of methanol (4.2.2) and 215 ml of water (4.2.3) and mix.
4.3.3 Stock solution of Bisphenol A in methanol at a defined concentration of approximately
0,38 mg/ml
Weigh to the nearest 0,1 mg approximately 37,5 mg of Bisphenol A (4.1.1) into a 100 ml volumetric
flask. Dissolve the Bisphenol A in methanol and make up to the mark with methanol (4.2.2).
Calculate the concentration in micrograms of Bisphenol A per millilitre of solution.
Repeat the procedure to obtain a second stock solution.
NOTE The solution can be stored in a well closed container in dark for a maximum of 3 months at any
temperature between +20 °C and –20 °C.
5 Apparatus
NOTE An instrument or item of apparatus is listed only where it is special or made to a particular
specification, usual laboratory glassware and equipment being assumed to be available.
5.1 High performance liquid chromatograph, preferably, equipped with an automatic 20 µl loop
injector and a variable wavelength UV detector connected to an i
...
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