EN 15786:2009
(Main)Animal feeding stuffs - Isolation and enumeration of Pediococcus spp.
Animal feeding stuffs - Isolation and enumeration of Pediococcus spp.
This European Standard defines general rules for the enumeration of probiotic pediococci in feed samples (additives, premixtures and feeding stuffs) that contain pediococci as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable for mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].
There are different categories of feed samples:
a) Additives containing about 1010 (colony forming units) CFU/g
b) Premixtures containing about 108 CFU/g
c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.
The detection limit is as defined in ISO 7218.
Futtermittel - Keimzählung von Pediococcus spp.
Diese Europäische Norm legt allgemeine Regeln für die Zählung von probiotischen Pediokokken in Futter-mittelproben (Zusatzstoffe, Vormischungen und Futtermittel) fest, die Pediokokken als einzelnen probiotischen Bestandteil oder in einem Gemisch mit anderen probiotischen Mikroorganismen enthalten. Diese Norm ist nicht anwendbar auf mineralische Futtermittel, die als Ergänzungsfuttermittel definiert sind, hauptsächlich aus Mineralien zusammengesetzt sind und mindestens 40 % Rohasche enthalten (Richtlinie 79/373/EWG) [3].
Es gibt unterschiedliche Kategorien von Futtermittelproben:
a) Zusatzstoffe, die etwa 1010 koloniebildende Einheiten (KbE/g) enthalten;
b) Vormischungen, die etwa 108 KbE/g enthalten;
c) Futtermittel, Mehl oder Pellets, die etwa 106 KbE/g enthalten, einschließlich Fertigfuttermittel und Milch¬austauscher.
Die Nachweisgrenze entspricht der in EN ISO 7218 festgelegten.
Aliments des animaux - Isolement et dénombrement du Pediococcus spp.
La présente norme internationale définit les règles générales de dénombrement des pédiocoques probiotiques dans les échantillons d’aliments (additif, prémélange et aliments des animaux) qui contiennent des pédiocoques comme seul composant bactérien ou dans un mélange comportant d’autres micro organismes. La présente norme ne s’applique pas aux aliments minéraux définis comme aliments complémentaires constitués principalement de minéraux et contenant au moins 40 % de cendre brute (Directive 79/373/CEE du Conseil).
Il existe différentes catégories d’échantillons d’aliments :
les additifs contenant environ 1010 UFC/g ;
les prémélanges contenant environ 108 UFC/g ;
les aliments, farines ou granulés qui contiennent environ 106 UFC/g et comprennent des aliments complets et des succédanés du lait.
Krma - Izolacija in štetje domnevno prisotnih Pediococcus spp
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Krma - Izolacija in štetje domnevno prisotnih Pediococcus sppFuttermittel - Keimzählung von Pediococcus sppAliments des animaux - Isolement et dénombrement du Pediococcus sppAnimal feeding stuffs - Isolation and enumeration of Pediococcus spp65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 15786:2009SIST EN 15786:2009en,fr,de01-december-2009SIST EN 15786:2009SLOVENSKI
STANDARD
SIST EN 15786:2009
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15786
September 2009 ICS 65.120 English Version
Animal feeding stuffs - Isolation and enumeration of Pediococcus spp.
Aliments des animaux - Isolement et dénombrement du Pediococcus spp.
Futtermittel - Keimzählung von Pediococcus spp. This European Standard was approved by CEN on 1 August 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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B-1000 Brussels © 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15786:2009: ESIST EN 15786:2009
EN 15786:2009 (E) 2 Contents Page Foreword .3 Introduction .4 1 Scope .5 2 Normative references .5 3 Terms and definitions .5 4 Principle .6 5 Diluent, selective media and phenotypic characterisation .6 6 Apparatus and glassware .8 7 Sampling . 10 8 Preparation of test sample . 10 9 Procedure . 10 10 Expression of results . 12 11 Precision . 12 12 Test report . 13 Annex A (informative) Notes on procedure . 14 Annex B (informative) Results of the interlaboratory study . 15 Bibliography . 17
SIST EN 15786:2009
EN 15786:2009 (E) 3 Foreword This document (EN 15786:2009) has been prepared by Technical Committee CEN/TC 327 “Animal feeding stuffs”, the secretaria
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