Organic, organo-mineral and inorganic fertilizers - Detection of Enterocococaceae

This document specifies a method for the detection and enumeration of Enterococcaceae in fertilizers of the following Product Function Categories (PFCs) of EU fertilizing products, as described in Regulation (EU) 2019/1009 [1]:
•   PFC 1(A): Organic fertilizer;
•   PFC 1(B): Organo-mineral fertilizer;
•   PFC 1(C): Inorganic fertilizer, which contains more than 1 % by mass of organic carbon, other than organic carbon from chelating or complexing agents, nitrification inhibitors, denitrification inhibitors or urease inhibitors, coating agents, urea or calcium cyanamide. The present method was validated on products known as present on the market in April 2021 and conform to Regulation (EU) 2019/1009 [1] that are inorganic fertilizers with more than 1 % of organic carbon such as poultry manure and struvite with low level of organic matter. In case that other products would be developed having other physical and chemical characteristics, it might become necessary to develop different methods to correctly account for pathogens they might contain.
This document specifies a colony-count technique on selective media, Slanetz Bartley agar or Bile Esculin Azide agar, respectively. The method is based on EN ISO 7899 2:2000.

Organische, organisch-mineralische und anorganische Düngemittel - Nachweis von Enterococcaceae

Dieses Dokument legt ein Verfahren zum Nachweis von Enterococcaceae in Düngemitteln der folgenden Produktfunktionskategorien (PFCs, en: Product Function Categories) von EU-Düngeprodukten fest, wie in der Verordnung (EU) 2019/1009 [1] beschrieben:
— PFC 1(A): organisches Düngemittel;
— PFC 1(B): organisch-mineralisches Düngemittel;
— PFC 1(C): anorganisches Düngemittel, das mehr als 1 % Masseanteil an organischem Kohlenstoff enthält, ausgenommen organischer Kohlenstoff aus Chelatbildnern oder Komplexbildnern, Nitrifikations-hemmstoffen, Denitrifikationshemmstoffen oder Ureasehemmstoffen, Hüllstoffen, Harnstoff oder Kalkstickstoff. Das vorliegende Verfahren wurde an Produkten validiert, von denen bekannt ist, dass sie im April 2021 auf dem Markt waren und der Verordnung (EU) 2019/1009 [1] entsprechen und bei denen es sich um anorganische Düngemittel mit mehr als 1 % organischem Kohlenstoff handelt, wie z. B. Geflügelmist und Struvit mit geringem Gehalt an organischer Substanz. Für den Fall, dass andere Produkte mit anderen physikalischen und chemischen Eigenschaften entwickelt werden, könnte es notwendig werden, andere Verfahren zu entwickeln, um die darin möglicherweise enthaltenen Pathogene korrekt zu erfassen.
Dieses Dokument legt ein Koloniezählverfahren auf Selektivmedien fest, Slanetz-Bartley-Agar bzw. Galle-Äsculin-Azid-Agar. Dieses Verfahren basiert auf EN ISO 7899-2:2000.

Engrais organiques, organo-minéraux et inorganiques - Recherche des Enterococaceae

Le présent document spécifie une méthode pour la recherche et le dénombrement des Enterococcaceae dans les engrais des catégories fonctionnelles de produits (PFC) des fertilisants UE décrites dans le règlement (UE) 2019/1009 [1] :
•   PFC 1(A) : engrais organique ;
•   PFC 1(B) : engrais organo-minéral ;
•   PFC 1(C) : engrais inorganique, contenant plus de 1 % en masse de carbone organique, autre que le carbone organique des agents chélatants ou complexants, des inhibiteurs de nitrification, des inhibiteurs de dénitrification ou des inhibiteurs d’uréase, des agents d’enrobage, de l’urée ou du cyanamide calcique. La présente méthode a été validée sur des produits connus comme présents sur le marché en avril 2021 et conformes au règlement (UE) 2019/1009 [1] qui sont des engrais inorganiques contenant plus de 1 % de carbone organique, tel que la poulaitte et la struvite avec un niveau bas de matière organique. Dans le cas où d'autres produits seraient développés avec d'autres caractéristiques physiques et chimiques, il pourrait s'avérer nécessaire de développer différentes méthodes pour rendre compte correctement des pathogènes qu'ils pourraient contenir.
Le présent document spécifie une technique de comptage des colonies sur des milieux sélectifs, la gélose Slanetz Bartley ou la gélose Bile-Esculine-Azoture, respectivement. La méthode est basée sur l’EN ISO 7899 2:2000.

Organska, organsko-mineralna in anorganska gnojila - Ugotavljanje prisotnosti enterokokov (Enterocococaceae)

General Information

Status
Published
Publication Date
17-May-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
18-May-2022
Due Date
04-Feb-2023
Completion Date
18-May-2022

Buy Standard

Technical specification
TS CEN/TS 17804:2024
English language
17 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day
Draft
kTS FprCEN/TS 17804:2022
English language
18 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 17804:2024
01-junij-2024
Organska, organsko-mineralna in anorganska gnojila - Ugotavljanje prisotnosti
enterokokov (Enterocococaceae)
Organic, organo-mineral and inorganic fertilizers - Detection of Enterocococaceae
Organische, organisch-mineralische und anorganische Düngemittel - Nachweis von
Enterococcaceae
Engrais organiques, organo-minéraux et inorganiques - Recherche des Enterococaceae
Ta slovenski standard je istoveten z: CEN/TS 17804:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17804:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST-TS CEN/TS 17804:2024

---------------------- Page: 2 ----------------------
SIST-TS CEN/TS 17804:2024
CEN/TS 17804
TECHNICAL SPECIFICATION
SPÉCIFICATION TECHNIQUE
May 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version
Organic, organo-mineral and inorganic fertilizers -
Detection of Enterocococaceae
Engrais organiques, organo-minéraux et inorganiques Organische, organisch-mineralische und
- Recherche des Enterococaceae anorganische Düngemittel - Nachweis von
Enterococcaceae
This Technical Specification (CEN/TS) was approved by CEN on 13 April 2022 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17804:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17804:2024
CEN/TS 17804:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Reagents . 7
5.1 General. 7
5.2 Diluents . 7
5.2.1 General. 7
5.2.2 Basic phosphate buffer . 7
5.2.3 Double-buffered phosphate buffer . 7
5.3 Selective media . 7
5.3.1 Slanetz Bartley agar .
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17804:2022
01-januar-2022
Organska, organsko-mineralna in anorganska gnojila - Ugotavljanje prisotnosti
enterokokov (Enterococcaceae)
Organic, organo-mineral and inorganic fertilizers - Detection of Enterococcaceae
Organische, organisch-mineralische und anorganische Düngemittel - Nachweis von
Enterococcaceae
Engrais organiques, organo-minéraux et inorganiques - Recherche des Enterococaceae
Ta slovenski standard je istoveten z: FprCEN/TS 17804
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17804:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17804:2022

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17804:2022


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17804
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

December 2021
ICS 65.080
English Version

Organic, organo-mineral and inorganic fertilizers -
Detection of Enterococcaceae
Engrais organiques, organo-minéraux et inorganiques - Organische, organisch-mineralische und anorganische
Recherche des Enterococaceae Düngemittel - Nachweis von Enterococcaceae


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 260.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17804:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17804:2022
FprCEN/TS 17804:2021(E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Reagents . 8
5.1 General. 8
5.2 Diluents . 8
5.2.1 General. 8
5.2.2 Basic phosphate buffer . 8
5.2.3 Double-buffered phosphate buffer . 8
5.3 Selective media . 8
5.3.1 Slanetz Bartley agar . 8
5.3.2 Bile Esculin Azide agar .
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.