Organic and organo-mineral fertilizers - Determination of the biuret content by high-performance liquid chromatography (HPLC)

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [4]. However, the present method was not validated for blends.
This document specifies a method for the determination of the biuret content by high-performance liquid chromatography (HPLC) with UV detector. The method is applicable to organic and organo-mineral fertilizers containing urea.
Nowadays, there is a method standardized as EN 15479 that allows the determination of biuret in urea by spectrophotometric detection. Organic and organo-mineral fertilizers contain organic matter and other compounds apart from urea that would interfere in a spectrophotometric method. HPLC allows an accurate determination of biuret by separating it from possible interfering compounds [2] [3].

Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts mittels Hochleistungs-Flüssigkeitschromatographie (HPLC)

Dieses Dokument ist für Düngeprodukte anzuwenden, die den Kategorien PFC 1(A) und PFC 1(B) zugeordnet oder als Komponente der Kategorien PFC 1(A) und PFC 1(B) in PFC 7 der Verordnung (EU) 2019/1009 [4] eingestuft sind. Das vorliegende Verfahren wurde jedoch nicht für Mischungen validiert.
Dieses Dokument legt ein Verfahren zur Bestimmung des Biuretgehalts mittels Hochleistungs-Flüssigkeitschromatographie (HPLC) mit UV Detektor fest. Das Verfahren ist für organische und organisch-mineralische Düngemittel, die Harnstoff enthalten, anzuwenden.
Heutzutage gibt es ein als EN 15479 genormtes Verfahren, das die Bestimmung von Biuret in Harnstoff durch spektrophotometrische Detektion ermöglicht. Organische und organisch-mineralische Düngemittel enthalten neben Harnstoff auch andere Verbindungen, die bei einem spektralphotometrischen Verfahren stören würden. Die HPLC ermöglicht eine genaue Bestimmung von Biuret, indem sie es von möglichen störenden Verbindungen trennt [2] [3].

Engrais organiques et organo-minéraux - Détermination de la teneur en biuret par chromatographie liquide à haute performance (HPLC)

Le présent document est applicable aux fertilisants qui sont classés dans les catégories PFC 1(A) et PFC 1(B) ou comme composants de PFC 1(A) et de PFC 1(B) dans la catégorie PFC 7 du Règlement (UE) 2019/1009 [4]. Toutefois, la présente méthode n’a pas été validée pour les mélanges.
Le présent document spécifie une méthode pour la détermination de la teneur en biuret par chromatographie en phase liquide haute performance (HPLC) avec un détecteur UV. La méthode est applicable aux engrais organiques et organo-minéraux contenant de l’urée.
De nos jours, il existe une méthode normalisée, l’EN 15479, qui permet de doser le biuret dans l’urée par détection spectrophotométrique. Cependant, les engrais organiques et organo-minéraux contiennent de la matière organique et des composés autres que l’urée qui interfèreraient en cas d’utilisation d’une méthode spectrophotométrique. La HPLC permet un dosage exact du biuret en le séparant des composés pouvant interférer [2] [3].

Organska in organsko-mineralna gnojila - Določevanje biureta s tekočinsko kromatografijo visoke ločljivosti (HPLC)

Ta dokument vzpostavlja metodologijo za določevanje vsebnosti biureta.

General Information

Status
Published
Publication Date
05-Apr-2022
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
06-Apr-2022
Due Date
04-Feb-2023
Completion Date
06-Apr-2022

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SLOVENSKI STANDARD
SIST-TS CEN/TS 17765:2023
01-februar-2023
Organska in organsko-mineralna gnojila - Določevanje biureta s tekočinsko
kromatografijo visoke ločljivosti (HPLC)
Organic and organo-mineral fertilizers - Determination of the biuret content by high-
performance liquid chromatography (HPLC)
Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts
mittels Hochleistungs-Flüssigkeitschromatographie (HPLC)
Engrais organiques et organo-minéraux - Détermination de la teneur en biuret par
chromatographie liquide à haute performance (HPLC)
Ta slovenski standard je istoveten z: CEN/TS 17765:2022
ICS:
65.080 Gnojila Fertilizers
SIST-TS CEN/TS 17765:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST-TS CEN/TS 17765:2023

---------------------- Page: 2 ----------------------
SIST-TS CEN/TS 17765:2023


CEN/TS 17765
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

April 2022
TECHNISCHE SPEZIFIKATION
ICS 65.080
English Version

Organic and organo-mineral fertilizers - Determination of
the biuret content by high-performance liquid
chromatography (HPLC)
Engrais organiques et organo-minéraux - Organische und organisch-mineralische Düngemittel -
Détermination de la teneur en biuret Bestimmung des Biuretgehalts
This Technical Specification (CEN/TS) was approved by CEN on 13 March 2022 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17765:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST-TS CEN/TS 17765:2023
CEN/TS 17765:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure . 6
7.1 Sampling . 6
7.2 Calibration solutions . 7
7.3 Preparation of the sample . 7
7.4 Extraction of the sample and preparation of the test solution. 7
7.5 HPLC conditions . 8
7.6 Preparation of the standard curve . 8
7.7 Determination of biuret in the test solutions . 8
8 Calculations . 9
9 Test report . 9
Bibliography . 10

2

---------------------- Page: 4 ----------------------
SIST-TS CEN/TS 17765:2023
CEN/TS 17765:2022 (E)
European foreword
This document (CEN/TS 17765:2022) has been prepared by Technical Committee CEN/TC 260
“Fertilizers and liming materials”, the secretariat of which is held by DIN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
3

---------------------- Page: 5 ----------------------
SIST-TS CEN/TS 17765:2023
CEN/TS 17765:2022 (E)
Introduction
Regulation (EU) 2019/1009 [4] lays down the rules on the making available on the market of EU
fertilizing products and the specific safety and quality requirements for the defined product function
categories (PFCs). Organic and organo-mineral fertilizers have been classified as PFC 1(A) and PFC 1(B).
This document defines test methods for the determination of the biuret content to be used for organic
and organo-mineral fertilizers in order to measure the compliance with the related requirement in
Regulation (EU) 2019/1009 [4].
When heating urea near or above its melting point (132 °C) during the manufacturing of urea, ammonia
slowly evolves and several different substances, including biuret, are formed. Excessively high
concentrations of biuret can have detrimental effects on plants growth.
4

---------------------- Page: 6 ----------------------
SIST-TS CEN/TS 17765:2023
CEN/TS 17765:2022 (E)
1 Scope
This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the
PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [4]. However, the present
method was not validated for blends.
This document specifies a method for the determination of the biuret content by high-performance liquid
chromatography (HPLC) with UV detector. The method is applicable to organic and organo-mineral
fertilizers containing urea.
Nowadays, there is a method standardized as EN 15479 that allows the determination of biuret in urea
by spectrophotometric detection. Organic and organo-mineral fertilizers contain organic matter and
other compounds apart from urea that would interfere in a spectrophotometric method. HPLC allows an
accurate determination o
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 17765:2021
01-december-2021
Organska in organsko-mineralna gnojila - Določevanje biureta
Organic and organo-mineral fertilizers - Determination of the biuret content
Organische und organisch-mineralische Düngemittel - Bestimmung des Biuretgehalts
Ta slovenski standard je istoveten z: FprCEN/TS 17765
ICS:
65.080 Gnojila Fertilizers
kSIST-TS FprCEN/TS 17765:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
kSIST-TS FprCEN/TS 17765:2021

---------------------- Page: 2 ----------------------
kSIST-TS FprCEN/TS 17765:2021


FINAL DRAFT
TECHNICAL SPECIFICATION
FprCEN/TS 17765
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

November 2021
ICS 65.080
English Version

Organic and organo-mineral fertilizers - Determination of
the biuret content
 Organische und organisch-mineralische Düngemittel -
Bestimmung des Biuretgehalts


This draft Technical Specification is submitted to CEN members for Vote. It has been drawn up by the Technical Committee
CEN/TC 260.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change
without notice and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 17765:2021 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
kSIST-TS FprCEN/TS 17765:2021
FprCEN/TS 17765:2021 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Procedure . 6
7.1 Sampling . 6
7.2 Calibration solutions . 7
7.3 Preparation of the sample . 7
7.4 Extraction of the sample and preparation of the test solution. 7
7.5 HPLC conditions . 8
7.6 Preparation of the standard curve . 8
7.7 Determination of biuret in the test solutions . 8
8 Calculations . 8
9 Test report . 9
Bibliography . 10

2

---------------------- Page: 4 ----------------------
kSIST-TS FprCEN/TS 17765:2021
FprCEN/TS 17765:2021 (E)
European foreword
This document (FprCEN/TS 17765:2021) has been prepared by Technical Committee CEN/TC 260
“Fertilizers and liming materials”, the secretariat of which is held by DIN.
This document is currently submitted to the Vote on TS.
This document has been prepared under a standardisation request given to CEN by the European
Commission and the European Free Trade Association.
3

---------------------- Page: 5 ----------------------
kSIST-TS FprCEN/TS 17765:2021
FprCEN/TS 17765:2021 (E)
Introduction
Regulation (EU) 2019/1009 [4] lays down the rules on the making available on the market of EU
fertilizing products and the specific safety and quality requirements for the defined product function
categories (PFCs). Organic and organo-mineral fertilizers have been classified as PFC 1(A) and PFC 1(B).
This document defines test methods for the determination of the biuret content to be used for organic
and organo-mineral fertilizers in order to measure the compliance with the related requirement in the
Regulation (EU) 2019/1009 [4].
When heating urea near or above its melting point (132 °C) during the manufacturing of urea, ammonia
slowly evolves and several different substances, including biuret, are formed. Excessively high
concentrations of biuret can have detrimental effects on plants growth.
4

---------------------- Page: 6 ----------------------
kSIST-TS FprCEN/TS 17765:2021
FprCEN/TS 17765:2021 (E)
1 Scope
This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the
PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [4]. However, the present
method was not validated for blends.
This document specifies a method for the determination of the biuret content by high-performance liquid
chromatography (HPLC) with UV detector. The method is applicable to organic and organo-mineral
fertilizers containing urea.
Nowadays, there is a method standardized as EN 15479 that allows the determination of biuret in urea
by spectrophotometric detection. Organic and organo-mineral fertilizers contain organic matter and
other compounds apart from urea that would interfere in a spectrophotometric method. HPLC allows an
accurate determination of biuret by separating it from possible interfering compounds [2] [3].
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696:1995, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC ma
...

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