EN 15784:2009
(Main)Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.
Animal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp.
This European Standard defines general rules for the enumeration of probiotic bacilli in feeds containing bacilli (Bacillus species) as a single microorganism, component or mixed with other microorganisms. This method is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].
There are different categories of feed samples:
a) Additives containing about 1010 colony forming units (CFU)/g;
b) Premixtures containing about 108 CFU/g;
c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.
The detection limits are 500 (5 x 102) colony forming units per gram (CFU/g). The limits of determination are 2 x 104 CFU/g.
Futtermittel - Keimzählung von Bacillus spp.
Diese Europäische Norm legt allgemeine Regeln für die Zählung von probiotischen Bacillus-Arten (Bacillus spp.) in Futtermitteln fest, die Bacillus-Arten als einzelnen probiotischen Bestandteil oder in einem Gemisch mit anderen probiotischen Mikroorganismen enthalten. Dieses Verfahren ist nicht anwendbar auf mineralische Futtermittel, die als Ergänzungsfuttermittel definiert sind, welche hauptsächlich aus Mineralien zusammen-gesetzt sind und mindestens 40 % Rohasche enthalten (Richtlinie 79/373/EWG) [3].
Es gibt unterschiedliche Kategorien von Futtermittelproben:
a) Zusatzstoffe, die etwa 1010 koloniebildende Einheiten (KbE/g) enthalten;
b) Vormischungen, die etwa 108 KbE/g enthalten;
c) Futtermittel, Mehl oder Pellets, die etwa 106 KbE/g enthalten, einschließlich Fertigfuttermittel und Milch-austauscher;
Die Nachweisgrenzen liegen bei 500 (5 102) koloniebildenden Einheiten je Gramm (KbE/g). Die Bestim-mungsgrenzen liegen bei 2 104 KbE/g.
Aliments des animaux - Isolement et dénombrement des souches de Bacillus spp. présumées
La présente Norme européenne définit des règles générales pour le dénombrement des bacilles probiotiques dans les aliments contenant des bacilles (espèces Bacillus) sous forme de micro-organisme unique, de composant ou mélangés à d’autres micro-organismes. La présente méthode ne s’applique pas aux aliments minéraux, qui se définissent comme des aliments complémentaires constitués principalement de minéraux et contenant au moins 40 % de cendre brute (Directive 79/373/CEE du Conseil) [3].
Il existe différentes catégories d’échantillons d’aliments :
a) les additifs contenant environ 1010 unités formant colonie (UFC)/g ;
b) les prémélanges contenant environ 108 UFC/g ;
c) les aliments, farines ou granulés qui contiennent environ 106 UFC/g et qui se composent d’aliments complets et d’aliments d’allaitement.
Les limites de détection sont de 500 (5 102) unités formant colonie par gramme (UFC/g). Les seuils de détermination sont de 2 104 UFC/g.
Krma - Izolacija in štetje domnevno prisotnih Bacillus spp
General Information
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Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Krma - Izolacija in štetje domnevno prisotnih Bacillus sppFuttermittel - Keimzählung von vermutlichen Bacillus sppAliments des animaux - Isolement et dénombrement des souches probiotiques de BacillusAnimal feeding stuffs - Isolation and enumeration of presumptive Bacillus spp65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 15784:2009SIST EN 15784:2009en,fr,de01-december-2009SIST EN 15784:2009SLOVENSKI
STANDARD
SIST EN 15784:2009
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 15784
September 2009 ICS 65.120 English Version
Animal feeding stuffs -Isolation and enumeration of presumptive Bacillus spp.
Aliments des animaux - Isolement et dénombrement des souches probiotiques de Bacillus spp.
Futtermittel - Keimzählung von Bacillus spp. This European Standard was approved by CEN on 1 August 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
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B-1000 Brussels © 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15784:2009: ESIST EN 15784:2009
EN 15784:2009 (E) 2 Contents Page Foreword .3 Introduction .4 1 Scope .5 2 Normative references .5 3 Terms and definitions .5 4 Principle .6 5 Diluents and selective medium .6 6 Apparatus and glassware .7 7 Sampling .8 8 Preparation of test sample .8 9 Procedure .8 10 Expression of results . 10 11 Precision . 11 12 Test report . 11 Annex A (informative) Notes on procedure . 12 Annex B (informative) Results of the interlaboratory study . 13 Bibliography . 14
SIST EN 15784:2009
EN 15784:2009 (E) 3 Foreword This document (EN 15784:2009) has been
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