EN 14352:2004

Foodstuffs - Determination of fumonisin B1 and B2 in maize based foods - HPLC method with immunoaffinity column clean up

This document specifies a method for the detemination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in maize based foods using high performance liquid chromatography (HPLC) and immunoaffinity clean-up, see [1], [2], [3].
The method has been successfully validated in a collaborative study according to AOAC Guidelines for collaborative study procedures [4] to validate characteristics of a method of analysis for the determination of fumonisins in maize flour and corn flakes containing 323 µg/kg to 1414 µg/kg FB1 and 90 µg/kg to 558 µg/kg FB2.

Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Maiserzeugnissen - HPLC-Vefahren mit Immunoaffinitätssäulen-Reinigung

Dieses Europäische Dokument legt ein Verfahren zur Bestimmung des Gehaltes von Fumonisin B1 (FB1) und Fumonisin B2 (FB2) in Maiserzeugnissen mit Hochleistungs-Flüssigchromatographie (HPLC) und Immunoaffinitätssäulen-Reinigung fest, siehe [1], [2] und [3].
Das Verfahren wurde erfolgreich in einem Ringversuch nach den "AOAC-Guidelines for Collaborative Studies to validate characteristics of a method of analysis" [4] für die Untersuchung von Fumonisinen in Maismehl und Cornflakes, die 323 µg/kg bis 1 414 µg/kg FB1 und 90 µg/kg bis 558 µg/kg FB2 enthalten, validiert.

Produits alimentaires - Dosage des fumonisines B1 et B2 dans des aliments à base de maïs - Méthode CLHP avec purification par colonne d'immunoaffinité

Le présent document spécifie une méthode de dosage de la fumonisine B1 (FB1) et de la fumonisine B2 (FB2) dans les aliments à base de maïs, par chromatographie en phase liquide haute performance (CLHP) et par purification par immunoaffinité (voir [1], [2], [3].
Cette méthode, validée par un essai interlaboratoires selon les Lignes Directrices AOAC pour Essais Interlaboratoires [4], consiste à doser les fumonisines dans la farine de maïs et les flocons de maïs contenant 323 µg/kg à 1414 µg/kg de FB1 et 90 µg/kg à 558 µg/kg de FB2.

Živila - Določevanje fumonizina B1 in B2 v živilih na osnovi koruze - Metoda HPLC s čiščenjem z imunoafinitetno kolono

General Information

• Status: Published
• Publication Date: 27-Jul-2004
• Withdrawal Date: 30-Jan-2005
• ICS: 67.060 - Cereals, pulses and derived products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 28-Oct-2020
• Ref Project: SIST EN 14352:2005 - Foodstuffs - Determination of fumonisin B1 and B2 in maize based foods - HPLC method with immunoaffinity column clean up

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Maiserzeugnissen - HPLC-Vefahren mit Immunoaffinitätssäulen-ReinigungProduits alimentaires - Dosage des fumonisines B1 et B2 dans des aliments a base de mais - Méthode CLHP avec purification par colonne d'immunoaffinitéFoodstuffs - Determination of fumonisin B1 and B2 in maize based foods - HPLC method with immunoaffinity column clean up67.060QMLKCereals, pulses and derived productsICS:Ta slovenski standard je istoveten z:EN 14352:2004SIST EN 14352:2005en01-januar-2005SIST EN 14352:2005SLOVENSKI
STANDARD



SIST EN 14352:2005



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14352July 2004ICS 67.060English versionFoodstuffs - Determination of fumonisin B1 and B2 in maizebased foods - HPLC method with immunoaffinity column cleanupProduits alimentaires - Dosage des fumonisines B1 et B2dans des aliments à base de maïs - Méthode CLHP avecpurification par colonne d'immunoaffinitéLebensmittel - Bestimmung von Fumonisin B1 und B2 inMaiserzeugnissen - HPLC-Vefahren mitImmunoaffinitätssäulen-ReinigungThis European Standard was approved by CEN on 30 April 2004.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2004 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14352:2004: ESIST EN 14352:2005



EN 14352:2004 (E) 2 Contents page Foreword.3 1 Scope.4 2 Normative references.4 3 Principle.4 4 Reagents.4 5 Apparatus.7 6 Sampling.8 7 Procedure.8 8 Calculation.10 9 Precision.10 10 Test report.12 Annex A (informative)
Precision data.14 Annex B (informative)
Typical chromatograms.16 Bibliography.18
SIST EN 14352:2005



EN 14352:2004 (E) 3 Foreword This document (EN 14352:2004) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard,
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