EN 47:2005
(Main)Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
Wood preservatives - Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
This document specifies a method for the determination of the toxic values of a wood preservative against the larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation.
This method is applicable to:
¾ water-insoluble chemicals which are being studied as active insecticides;
¾ organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
¾ organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates;
¾ water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures.
Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Larven von Hylotrupes bajulus (Linnaeus) - (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Larven von Hylotrupes bajulus (Linnaeus) fest, die in Holz eingesetzt wurden, das zuvor durch Volltränkung behandelt wurde.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
- wasserlösliche Stoffe, zum Beispiel Salze.
Das Verfahren gilt unabhängig davon, ob die Proben einer angemessenen Alterungsbeanspruchung ausgesetzt wurden oder nicht.
Produits de préservation du bois - Détermination du seuil d'efficacité contre les larves d'Hylotrupes bajulus (Linnaeus) - (Méthode de laboratoire)
La présente Norme européenne prescrit une méthode de détermination du seuil d'efficacité d'un produit de préservation du bois contre les larves d'Hylotrupes bajulus (Linnaeus) après leur introduction dans du bois totalement imprégné.
La présente méthode est applicable :
¾ aux produits chimiques non hydrosolubles étudiés en tant que matières actives insecticides, ou
¾ aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés, ou
¾ aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés, ou
¾ aux produits solubles dans l'eau, par exemple aux sels.
Cette méthode s'applique à des éprouvettes d'essai ayant subi ou non des épreuves de vieillissement appropriées.
Zaščitna sredstva za les - Ugotavljanje toksičnih vrednosti proti ličinkam hišnega kozlička Hylotrupes bajulus (Linnaeus) (Laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2005
1DGRPHãþD
SIST EN 47:1996
SIST EN 47:1996/AC:2002
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHWRNVLþQLKYUHGQRVWLSURWLOLþLQNDPKLãQHJD
NR]OLþND+\ORWUXSHVEDMXOXV /LQQDHXV /DERUDWRULMVNDPHWRGD
Wood preservatives - Determination of the toxic values against larvae of Hylotrupes
bajulus (Linnaeus) - (Laboratory method)
Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Larven von
Hylotrupes bajulus (Linnaeus) - (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination du seuil d'efficacité contre les larves
d'Hylotrupes bajulus (Linnaeus) - (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 47:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN 47
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 47:1988
English version
Wood preservatives - Determination of the toxic values against
larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method)
Produits de préservation des bois - Détermination des Holzschutzmittel - Bestimmung der Grenze der
valeurs toxiques contre les larves d'Hylotrupes bajulus Wirksamkeit gegenüber Larven von Hylotrupes bajulus
(Linnaeus) - (Méthode de laboratoire) (Linnaeus) - (Laborverfahren)
This European Standard was approved by CEN on 3 February 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 47:2005: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative reference .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.8
7 Test specimens.9
8 Procedure .10
9 Expression of results .13
10 Test report .14
Annex A (informative) Example of a test report .16
Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) .18
Annex C (normative) Differentiation of heartwood and sapwood in Pinus species.21
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory .22
Bibliography .23
Foreword
This document (EN 47:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and
wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by September 2005, and conflicting national standards shall be
withdrawn at the latest by September 2005.
This document supersedes EN 47:1988.
Significant technical differences between this document and EN 47:1988 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.
Introduction
This document describes a laboratory method of testing which gives a basis for the general assessment of the
effectiveness of a wood preservative against Hylotrupes bajulus by determination and comparison with
different classes of larvae, of the concentration at which the product prevents their survival in totally
impregnated wood of a susceptible species.
In this respect it differs from the method specified in EN 46-1 which is intended to determine whether a
preservative applied to the surface is capable of preventing infestation of wood by these larvae.
This laboratory method provides a criterion by which the value of a preservative can be assessed. In making
this assessment the methods by which the preservative may be applied should be taken into account. It is
further recommended that results from this test should be supplemented by those from other appropriate tests
and, above all, by comparison with practical experience.
When products which are very active at very low concentration are used, it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other products,
treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate
rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see
also Annex E for environmental, health and safety precautions).
1 Scope
This document specifies a method for the determination of the toxic values of a wood preservative against the
larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation.
This method is applicable to:
water-insoluble chemicals which are being studied as active insecticides;
organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates; and
water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative reference
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the
total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Impregnation of several sets of test specimens of susceptible wood species with a series of concentrations of
the preservative.
Introduction of Hylotrupes bajulus larvae of a given category into these test specimens and determination of
their survival rate at fixed intervals of time.
Comparison of the results with those obtained with untreated and solvent or diluent-treated control test
specimens. Derivation of the toxic values of the product under test for the category of larvae in question.
5 Test materials
5.1 Biological material
5.1.1 Hylotrupes bajulus (Linnaeus) larvae
Category 1 (obligatory test): larvae within a maximum of 3 days of hatching.
Category 2 (optional additional test): larvae with masses in the range 50 mg to 150 mg.
5.1.2 Source of larvae
The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
NOTE Otherwise larvae in Category 2 can be taken from naturally infested wood, in which case they should be
transferred into sapwood of pine and stored for at least 4 weeks under the rearing conditions specified in Annex B.
Do not use the larvae in the test if they have not fed normally during this storage period.
5.1.3 Provision of larvae
Collect larvae in Category 1 from eggs laid by different females.
Carefully cut out the larvae in Category 2 from the culture blocks and keep them separated from one another
for 2 days to 3 days in the culturing chamber (5.3.1) to check that they are healthy.
5.1.4 Choice of larvae in Category 2
Use only healthy larvae in the test.
NOTE A healthy larva can be recognized by its ivory-white colour, its firm consistency and rounded appearance, and
by the absence of wounds or bites which show up as dark marks. Healthy larvae react to the touch by vigorous movement
and attempts to bite.
Reject any larvae which are shrunken or aged, which have recently moulted, or which are in a pre-pupal stage.
5.1.5 Number of larvae
The number of larvae per treated and control test specimen shall be six of Category 1 or one of Category 2.
Sort the larvae retained in Category 2 mentioned above.
Do not use larvae weighing more than 150 mg as they may pupate and therefore interfere with the test.
For a single test, use a mixed batch of larvae of Category 1 and for Category 2, as far as possible, use larvae
of similar masses. The number of larvae necessary is given in Table 1.
Table 1 – Number of larvae and test specimens
Larvae in Category 2
Concentrations Larvae in Category 1
a
Without radiography
With radiography
of
Type of test
preservatives
specimen
Number of Number of Number of
Number Number Number
test test test
Mass fraction
of larvae of larvae of larvae
specimens specimens specimens
Treated test
1 5 30 10 10 7 7
specimens
_ 2 5 30 10 10 7 7
_ 3 5 30 10 10 7 7
_ 4 5 30 10 10 7 7
_ 5 5 30 10 10 7 7
etc.
Untreated control test 0 5 30 10 10 7 7
specimens
Solvent or diluent 0 5 30 10 10 7 7
control test specimens
(including water)
Total for 5 concentrations 35 210 70 70 49 49
a
The use of radiography is only recommended in the case of tests with larvae in Category 2.
5.2 Products and reagents
5.2.1 Xylene, technical grade, mixed isomers.
5.2.2 Water, complying with grade 3 of EN ISO 3696.
5.2.3 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a
residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.4 Cellulose or absorbent cotton wool and filter paper
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at relative humidity
(85 ± 5) %.
5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at relative humidity
(65 ± 5) %.
NOTE The conditioning of test specimens can be carried out in the laboratory work area (see 5.3.3) provided that this
has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials.
Avoid excessive exposure of operators to solvents or their
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