CEN/TR 15641:2007
(Main)Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass spectrometric parameters
Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass spectrometric parameters
This Technical Report lists mass spectrometric parameters which are useful for the application of European Standards for the determination of pesticide residues in foods of plant origin that use LC-MS/MS, such as the standards in preparation:
prEN 15637 "Foods of plant origin — Determination of pesticide residues using LC-MS/MS following methanol extraction and clean up using diatomaceous earth"
prEN 15662 "Foods of plant origin — Determination of pesticide residues using GC-MS and/or LC-MS/MS following acetonitrile extraction/partitioning by dispersive SPE — QuEChERS-method”
To facilitate the determination of active substances and/or metabolites using LC-MS/MS, Table 1 specifies the precursor ions and product ions suitable for quantification, which can be used independently of the type of triple quadrupole mass spectrometer. However, using an ion trap mass spectrometer other product ions can be generated or at least the relative intensities of the ions are different to triple quadrupole instruments. Furthermore, the additional parameters declustering potential (DP), collision energy (CE), relative retention times and an approximate classification of detection sensitivity are presented in Table 1. These were derived using the API 2000 ) and should be applicable at least for other instruments of the API type (Applied Biosystems).
Lebensmitteluntersuchung - Bestimmung von Pestizidrückständen mit LC-MS/MS - Parameter für die Tandem-Massenspektrometrie
Analyse des produits alimentaires - Détermination des résidus de pesticides par CL-SM/SM - Paramètres de spectrométrie de masse en tandem
Analize živil - Določevanje ostankov pesticidov z LC-MS/MS - Parametri tandemske masne spektrometrije
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST-TP CEN/TR 15641:2007
01-november-2007
$QDOL]HåLYLO'RORþHYDQMHRVWDQNRYSHVWLFLGRY]/&06063DUDPHWULWDQGHPVNH
PDVQHVSHNWURPHWULMH
Food analysis - Determination of pesticide residues by LC-MS/MS - Tandem mass
spectrometric parameters
Lebensmitteluntersuchung - Bestimmung von Pestizidrückständen mit LC-MS/MS -
Parameter für die Tandem-Massenspektrometrie
Analyse des produits alimentaires - Détermination des résidus de pesticides par CL-
SM/SM - Parametres de spectrométrie de masse en tandem
Ta slovenski standard je istoveten z: CEN/TR 15641:2007
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST-TP CEN/TR 15641:2007 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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TECHNICAL REPORT
CEN/TR 15641
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
August 2007
ICS 67.050
English Version
Food analysis - Determination of pesticide residues by LC-
MS/MS - Tandem mass spectrometric parameters
Analyse des produits alimentaires - Détermination des Lebensmitteluntersuchung - Bestimmung von
résidus de pesticides par CL-SM/SM - Paramètres de Pestizidrückständen mit LC-MS/MS - Parameter für die
spectrométrie de masse en tandem Tandem-Massenspektrometrie
This Technical Report was approved by CEN on 25 July 2007. It has been drawn up by the Technical Committee CEN/TC 275.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 15641:2007: E
worldwide for CEN national Members.
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CEN/TR 15641:2007 (E)
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Analyte specific parameters for selective reaction monitoring of pesticides.5
2.1 General.5
2.2 LC Parameters.6
2.3 General MS/MS parameters .6
2.4 Analyte specific MS/MS Parameters.7
Bibliography .23
2
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CEN/TR 15641:2007 (E)
Foreword
This document (CEN/TR 15641:2007) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN.
3
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CEN/TR 15641:2007 (E)
Introduction
Pesticide residue analysis employs multi methods involving extraction of residues from foods and clean up of
the extract to obtain as many pesticide residues as possible in the purified extracts. Afterwards the extracts
can be analysed by different kinds of instruments.
The hyphenation of liquid chromatography (LC) and tandem mass spectrometry (MS/MS) has become one of
the most universal, but selective and sensitive analysis techniques for identification and quantification of
pesticide residues in extracts of foods.
For the ionization of the analytes (M) in LC-MS/MS, electro spray ionization (ESI) or atmospheric pressure
chemical ionization (APCI) interfaces are most commonly used. Depending on the selected polarity of the
+ -
ionization, protonated or deprotonated molecular ions like [M+H] or [M-H] are generated. Using ESI,
relatively stable adducts (clusters) of the analytes (M) and components of the mobile phase like ions (e.g.
ammonium, sodium or potassium ions) or solve
...
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