EN 16695:2015

Water quality - Guidance on the estimation of phytoplankton biovolume

This European Standard specifies a procedure for the estimation of biovolume of marine and freshwater phytoplankton taxa using inverted microscopy (Utermöhl technique according to EN 15204), in consideration of some heterotrophic protists (< 100 µm) that are not considered in routine zooplankton analysis and benthic microalgae, which can be found in pelagic water samples.
This European Standard describes the necessary methods for measuring cell dimensions and for the calculation of cell or counting unit volumes to estimate the biovolume in phytoplankton samples. This shall be done using harmonized assignments of geometrical shapes to avoid errors.

Wasserbeschaffenheit - Anleitung zur Abschätzung des Phytoplankton-Biovolumens

Diese Europäische Norm legt ein Verfahren zur Abschätzung des Biovolumens von marinen und limnischen Phytoplankton Taxa unter Anwendung der Umkehrmikroskopie (Utermöhl-Technik) nach EN 15204 fest, und berücksichtigt dabei einige heterotrophe Protisten (< 100 µm), die bei Routineauswertungen von Zooplankton nicht mit angerechnet werden und bentische Mikroalgen, die in pelagischen Wasserproben gefunden werden können.
Diese Europäische Norm beschreibt die erforderlichen Verfahren zur Messung der Zelldimensionen und zur Berechnung des Volumens von Zellen oder Zähleinheiten zur Abschätzung des Biovolumens in Phytoplanktonproben. Um Fehler zu vermeiden, werden harmonisierte geometrische Formen verwendet.

Qualité de l'eau - Lignes directrices pour l'estimation du biovolume des microalgues

La présente Norme européenne spécifie une méthode permettant d'estimer le biovolume des taxons de phytoplancton marin et d'eau douce par microscopie inversée (technique d'Utermöhl selon l'EN 15204), en tenant compte du fait que certains protistes hétérotrophes (< 100 µm), qui ne sont pas pris en considération dans l'analyse de routine du zooplancton et des microalgues benthiques, peuvent se trouver dans des échantillons d'eaux pélagiques.
La présente Norme européenne décrit les méthodes nécessaires pour mesurer les dimensions des cellules et pour calculer les volumes de cellules ou d'unités de comptage afin d'estimer le biovolume d'échantillons de phytoplancton. Pour cela, il est nécessaire d'utiliser des attributions harmonisées de formes géométriques afin d'éviter les erreurs.

Kakovost vode - Navodilo za ocenjevanje biovolumna mikroalg

Razvoj usklajenega protokola za oceno biovolumna alg, vključno s priporočenim seznamom geometričnih oblik najpogostejših evropskih taksonov fitoplanktona, ki izpolnjujejo zahteve iz okvirne direktive o vodah.
Metode naj bi zagotovile:
– mikroskopsko tehniko za merjenje dimenzij celic alg, ki so potrebne za oceno biovolumna fitoplanktona različnih taksonov fitoplanktona, vključno s posamičnimi celicami, oblikami kompleksnih celic in kolonijami;
– računske postopke za oceno biovolumna alg, vključno z razmerji biomase;
– potrebne postopke za zagotavljanje kakovosti;
– navodila za priporočene geometrične oblike za različne taksone fitoplanktona in pripadajoče enačbe za izračun biovolumna.

General Information

• Status: Published
• Publication Date: 01-Sep-2015
• Withdrawal Date: 30-Mar-2016
• ICS: 13.060.70 - Examination of biological properties of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 09-Feb-2022
• Directive: 2000/60/EC - Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy (substitutes Directive Reference 01/991 - Common Position on WFD)
• Mandate: M/424 - Mandate for standardisation addressed to CEN for the development or improvement standards in support of the water framework directive
• Ref Project: SIST EN 16695:2015 - Water quality - Guidance on the estimation of microalgal biovolume

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 16695:2015
01-november-2015
Kakovost vode - Navodilo za ocenjevanje biovolumna mikroalg
Water quality - Guidance on the estimation of microalgal biovolume
Wasserbeschaffenheit - Anleitung zur Abschätzung des Phytoplankton-Biovolumens
Qualité de l'eau - Lignes directrices pour l'estimation du biovolume des microalgues
Ta slovenski standard je istoveten z: EN 16695:2015
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
SIST EN 16695:2015 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 16695:2015

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SIST EN 16695:2015


EN 16695
EUROPEAN STANDARD

NORME EUROPÉENNE

September 2015
EUROPÄISCHE NORM
ICS 13.060.70
English Version

Water quality - Guidance on the estimation of
phytoplankton biovolume
Qualité de l'eau - Lignes directrices pour l'estimation Wasserbeschaffenheit - Anleitung zur Abschätzung des
du biovolume des microalgues Phytoplankton-Biovolumens
This European Standard was approved by CEN on 10 July 2015.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2015 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 16695:2015 E
worldwide for CEN national Members.

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SIST EN 16695:2015
EN 16695:2015 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Equipment and preservatives . 6
6 Procedure. 7
6.1 Sampling and sample preparation . 7
6.2 Calibration of the eyepiece micrometre, counting-graticule and image analysis
software . 7
6.3 Statistical requirements for determination . 8
6.4 Measurement . 9
6.4.1 General . 9
6.4.2 Using size classes based o
...