Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants (ISO 11269-2:2012)

This part of ISO 11269 describes a method to assess the quality of an unknown soil and the soil habitat function
by determining the emergence and early growth response of at least two terrestrial plant species compared
to reference or standard control soils. It is applicable to soils of unknown quality, e.g. from contaminated sites,
amended soils or soils after remediation.

Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora - Teil 2: Wirkung von verunreinigten Böden auf Saatauflauf und frühes Wachstum höherer Pflanzen (ISO 11269-2:2012)

Dieser Teil von ISO 11269 beschreibt ein Verfahren für die Beurteilung der Beschaffenheit eines unbekannten Bodens und der Lebensraumfunktion des Bodens durch Bestimmung der Wirkung auf den Saatauflauf und das frühe Wachstum von mindestens zwei terrestrischen Pflanzenarten im Vergleich zu Referenz- oder Standardkontrollböden. Das Verfahren ist auf Böden unbekannter Beschaffenheit, z. B. von kontaminierten Standorten, geänderte (verbesserte) Böden oder Böden nach einer Sanierung anwendbar.

Qualité du sol - Détermination des effets des polluants sur la flore du sol - Partie 2: Effets des sols contaminés sur l'émergence et la croissance des végétaux supérieurs (ISO 11269-2:2012)

La présente partie de l'ISO 11269 décrit une méthode d'évaluation de la qualité d'un sol inconnu et de la fonction
d'habitat d'un sol en déterminant l'émergence et la réponse sur les premiers stades de croissance d'au moins
deux espèces de végétaux terrestres par rapport à des sols de référence ou à des sols témoins standards. Elle
est applicable à des sols de qualité inconnue, par exemple des sols provenant de sites contaminés, des sols
amendés ou des sols après réhabilitation.

Kakovost tal - Vpliv onesnaževal na talno floro - 2. del: Vpliv onesnaženih tal na kalitev in rast višjih rastlin (ISO 11269-2:2012)

Ta del standarda ISO 11269 opisuje metodo za oceno kakovosti neznanih tal in funkcije habitata tal z ugotavljanjem kalitve in rasti vsaj dveh kopenskih rastlinskih vrst v primerjavi z referenčno ali standardno kontrolno prstjo. Uporablja se za tla neznane kakovosti, npr. tla z onesnaženih lokacij, obogatena tla ali tla po sanaciji.

General Information

Status
Published
Publication Date
19-Feb-2013
Withdrawal Date
30-Aug-2013
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
20-Feb-2013
Due Date
16-Jun-2013
Completion Date
20-Feb-2013

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SLOVENSKI STANDARD
01-maj-2013
1DGRPHãþD
SIST ISO 11269-2:2006
Kakovost tal - Vpliv onesnaževal na talno floro - 2. del: Vpliv onesnaženih tal na
kalitev in rast višjih rastlin (ISO 11269-2:2012)
Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of
contaminated soil on the emergence and early growth of higher plants (ISO 11269-
2:2012)
Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora
- Teil 2: Wirkung von kontaminierten Böden auf Saatauflauf und frühes Wachstum
höherer Pflanzen (ISO 11269-2:2012)
Qualité du sol - Détermination des effets des polluants sur la flore du sol - Partie 2: Effets
des sols contaminés sur l'émergence et la croissance des végétaux supérieurs (ISO
11269-2:2012)
Ta slovenski standard je istoveten z: EN ISO 11269-2:2013
ICS:
13.080.30 Biološke lastnosti tal Biological properties of soils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 11269-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2013
ICS 13.080.30
English Version
Soil quality - Determination of the effects of pollutants on soil
flora - Part 2: Effects of contaminated soil on the emergence and
early growth of higher plants (ISO 11269-2:2012)
Qualité du sol - Détermination des effets des polluants sur Bodenbeschaffenheit - Bestimmung der Wirkungen von
la flore du sol - Partie 2: Effets des sols contaminés sur Schadstoffen auf die Bodenflora - Teil 2: Wirkung von
l'émergence et la croissance des végétaux supérieurs (ISO verunreinigten Böden auf Saatauflauf und frühes
11269-2:2012) Wachstum höherer Pflanzen (ISO 11269-2:2012)
This European Standard was approved by CEN on 5 February 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11269-2:2013: E
worldwide for CEN national Members.

Contents Page
Foreword . 3
Foreword
The text of ISO 11269-2:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 11269-2:2013 by
Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2013, and conflicting national standards shall be withdrawn at
the latest by August 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11269-2:2012 has been approved by CEN as EN ISO 11269-2:2013 without any modification.

INTERNATIONAL ISO
STANDARD 11269-2
Third edition
2012-01-15
Soil quality — Determination of the
effects of pollutants on soil flora —
Part 2:
Effects of contaminated soil on the
emergence and early growth of higher
plants
Qualité du sol — Détermination des effets des polluants sur la flore du
sol —
Partie 2: Effets des sols contaminés sur l’émergence et la croissance
des végétaux supérieurs
Reference number
ISO 11269-2:2012(E)
©
ISO 2012
ISO 11269-2:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2012 – All rights reserved

ISO 11269-2:2012(E)
Contents Page
Foreword .iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Units . 3
5 Principle . 3
6 Test plants . 3
7 Materials . 4
7.1 Test vessels . 4
7.2 Soil . 4
8 Equipment . 6
9 Reference substance . 6
10 Procedure . 6
10.1 Experimental design . 6
10.2 Preparation of the pots . 7
10.3 Preparation of the seeds . 7
10.4 Growth conditions . 8
10.5 Start of the test . 8
10.6 Handling during the test . 8
11 Validity criteria . 9
12 Assessment of the results . 9
12.1 Data presentation . 9
12.2 Expression of the results . 9
13 Statistical analysis . 9
13.1 General . 9
13.2 Range-finding test .10
13.3 Final test .10
14 Test report . 11
Annex A (informative) Additional recommended plant species based on test results gained by applying
[4]
Environment Canada Test Method: EPS 1/RM/45 .13
Annex B (informative) Phytotoxic values for reference compounds: sodium trichloro-acetate
and boric acid .15
Annex C (informative) Recommended method for the measuring of the water-holding capacity
of the soil .16
Annex D (informative) Recommendations for nutrient supply of soils .17
Bibliography .18
ISO 11269-2:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11269-2 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4,
Biological methods.
This third edition cancels and replaces the second edition (ISO 11269-2:2005), which has been technically revised.
ISO 11269 consists of the following parts, under the general title Soil quality — Determination of the effects of
pollutants on soil flora:
— Part 1: Method for the measurement of inhibition of root growth
— Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
iv © ISO 2012 – All rights reserved

ISO 11269-2:2012(E)
Introduction
This part of ISO 11269 describes a procedure for evaluating the quality of soils of different origin carrying
unknown contaminations. The evaluation of the effects on plant growth is based on emergence and inh
...

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