Foodstuffs - Determination of vitamin K1 by HPLC

This draft European Standard specifies a method for the determination of vitamin K1 in foodstuffs by high performance liquid chromatography (HPLC). The determination of Vitamin K1 content is carried out by measurement of reduced phylloquinone. The method has been validated for milk and milk products, however laboratory experiences exist which show that the method is also applicable to other type of foodstuffs.

Lebensmittel - Bestimmung von Vitamin K1 mit HPLC

Diese Europäische Norm legt ein Verfahren zur Bestimmung von Vitamin K1 in Lebensmitteln mit Hochleistungsflüssigchromatographie (HPLC) fest. Der Gehalt an Vitamin K1 wird durch die Messung von reduziertem Phyllochinon bestimmt. Das Verfahren wurde für Milch und Milchprodukte validiert, es gibt jedoch experimentelle Labor-Erfahrungen, die zeigen, dass das Verfahren auch auf andere Lebensmittel anwendbar ist.

Produits alimentaires - Dosage de la vitamine K1 par CLHP

La présente Norme européenne spécifie une méthode de dosage de la vitamine K1 présente dans les produits alimentaires par chromatographie liquide haute performance (CLHP). La détermination de la teneur en vitamine K1 est effectuée par mesurage de la forme réduite de la phylloquinone. Cette méthode a été validée pour le lait et les produits laitiers pour nourrissons. Toutefois, des expériences en laboratoire ont montré que cette méthode est également applicable aux autres catégories de produits alimentaires [10].

Živila – Določevanje vitamina K1 s HPLC

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Vitamin K1 mit HPLCProduits alimentaires - Dosage de la vitamine K1 par CLHPFoodstuffs - Determination of vitamin K1 by HPLC67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 14148:2003SIST EN 14148:2003en01-november-2003SIST EN 14148:2003SLOVENSKI

SIST EN 14148:2003

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14148July 2003ICS 67.050English versionFoodstuffs - Determination of vitamin K1 by HPLCProduits alimentaires - Dosage de la vitamine K1 par CLHPLebensmittel - Bestimmung von Vitamin K1 mit HPLCThis European Standard was approved by CEN on 2 May 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14148:2003 ESIST EN 14148:2003

EN 14148:2003 (E)2ContentspageForeword.31Scope.42Normative references.43Principle.44Reagents.45Apparatus.66Procedure.77Calculation.88Precision.99Test report.10Annex A (informative)
Figures.11Annex B (informative)
Precision data.13Annex C (informative)
Alternative HPLC-Systems.14Bibliography.15SIST EN 14148:2003

EN 14148:2003 (E)3ForewordThis document (EN 14148:2003) has been prepared by Technical Committee CEN/TC 275 “Food analysis -Horizontal methods”, the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identical text orby endorsement, at the latest by January 2004, and conflicting national standards shall be withdrawn at the latestby January 2004.Annexes A, B and C are informative.WARNING — The use of this standard can involve hazardous materials, operations and equipment. Thisstandard does not purport to address all the safety problems associate

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