prEN 117
(Main)Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)
Wood preservatives - Determination of toxic values against Reticulitermes species (European termites) (Laboratory method)
This document specifies a method for the determination of the toxic values of a wood preservative against the Reticulitermes species of European termites.
This method is applicable to:
- water-insoluble chemicals which are being studied as active insecticides;
- organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and
- water-soluble materials, for example salts.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Reticulitermes-Arten (Europäische Termiten) (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der Grenze der Wirksamkeit eines Holzschutzmittels gegenüber Reticulitermes-Arten von europäischen Termiten fest.
Das Verfahren ist anzuwenden für:
- nicht wasserlösliche chemische Erzeugnisse, die als insektizide Wirkstoffe untersucht werden;
- organische, in Wasser dispergierbare Formulierungen im Anlieferungszustand oder in der Form, wie sie im Labor durch Verdünnung aus Konzentraten hergestellt werden; und
- wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG Das Verfahren kann in Verbindung mit Alterungsverfahren, zum Beispiel nach EN 73 oder EN 84, angewendet werden.
Produit de préservation du bois - Détermination du seuil d'efficacité contre les termites européens du genre Reticulitermes (Méthode de laboratoire)
Le présent document décrit une méthode de détermination du seuil d'efficacité d'un produit de préservation du bois contre les espèces de termites européens du genre Reticulitermes .
Cette méthode s'applique :
- aux produits chimiques non hydrosolubles étudiés en tant que matières actives ;
- aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ; et
- aux produits hydrosolubles, par exemple des sels.
NOTE Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple selon l'EN 73 ou l'EN 84.
Zaščitna sredstva za les - Ugotavljanje toksičnih vrednosti proti Reticulitermes santonensis (evropskim termitom) (laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 117:2022
01-december-2022
Zaščitna sredstva za les - Ugotavljanje toksičnih vrednosti proti Reticulitermes
santonensis (evropskim termitom) (laboratorijska metoda)
Wood preservatives - Determination of toxic values against Reticulitermes species
(European termites) (Laboratory method)Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenüber Reticulitermes-
Arten (Europäische Termiten) (Laboratoriumsverfahren)Produit de préservation du bois - Détermination du seuil d'efficacité contre les termites
européens du genre Reticulitermes (Méthode de laboratoire)Ta slovenski standard je istoveten z: prEN 117
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
oSIST prEN 117:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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oSIST prEN 117:2022
DRAFT
EUROPEAN STANDARD
prEN 117
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2022
ICS 71.100.50 Will supersede EN 117:2012
English Version
Wood preservatives - Determination of toxic values
against Reticulitermes species (European termites)
(Laboratory method)
Produit de préservation du bois - Détermination du Holzschutzmittel - Bestimmung der Grenze der
seuil d'efficacité contre les termites européens du Wirksamkeit gegenüber Reticulitermes-Arten
genre Reticulitermes (Méthode de laboratoire) (Europäische Termiten) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 117:2022 E
worldwide for CEN national Members.---------------------- Page: 3 ----------------------
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Contents Page
European foreword ....................................................................................................................................................... 3
Introduction .................................................................................................................................................................... 4
1 Scope .................................................................................................................................................................... 5
2 Normative references .................................................................................................................................... 5
3 Terms and definitions ................................................................................................................................... 5
4 Principle ............................................................................................................................................................. 5
5 Test materials ................................................................................................................................................... 6
5.1 Biological material .......................................................................................................................................... 6
5.2 Products and reagents ................................................................................................................................... 6
5.3 Apparatus ........................................................................................................................................................... 7
6 Sampling ............................................................................................................................................................. 8
7 Test specimens ................................................................................................................................................. 8
7.1 Species of wood ................................................................................................................................................ 8
7.2 Wood quality ..................................................................................................................................................... 8
7.3 Provision of test specimens ......................................................................................................................... 8
7.4 Dimensions of test specimens .................................................................................................................... 8
7.5 Number and distribution of test specimens .......................................................................................... 9
8 Procedure........................................................................................................................................................... 9
8.1 Preparation of test specimens .................................................................................................................... 9
8.1.1 Conditioning of test specimens prior to treatment ............................................................................ 9
8.1.2 Treatment of test specimens ....................................................................................................................... 9
8.1.3 Drying and conditioning of the test specimens after treatment ................................................. 10
8.2 Exposure of the test specimens to the insects ................................................................................... 10
8.2.1 Collecting and selecting the termites .................................................................................................... 10
8.2.2 Instalaltion of the termites ....................................................................................................................... 11
8.2.3 Exposure .......................................................................................................................................................... 12
8.3 Conditions and duration of test .............................................................................................................. 13
8.3.1 Exposure .......................................................................................................................................................... 13
8.3.2 Maintaining of moisture content ............................................................................................................ 13
8.4 Examination of the test specimens and colonies .............................................................................. 14
8.4.1 Assessments ................................................................................................................................................... 14
9 Expression of results ................................................................................................................................... 15
10 Test report ...................................................................................................................................................... 15
Annex A (informative) Example of a test report ............................................................................................ 17
Annex B (informative) Example of a method of culturing termites ........................................................ 19
Annex C (informative) Environmental, health and safety precautions withinchemical/biological laboratory .............................................................................................................. 20
Bibliography ................................................................................................................................................................. 21
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European foreword
This document (prEN 117:2022) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.This document is currently submitted to the CEN Enquiry.
This document will supersede EN 117:2012.
Significant technical differences between this document and EN 117:2012 are as follows:
a) the required purity of fine white quartz sand was lowered (5.2.1.1);b) the values for the examination of termite attack in rating 2i) and 3i) were slightly changed to close
rating gaps (8.4.1.2).NOTE 1 The species name “Reticulitermes santonensis” is officially withdrawn and replaced by Reticulitermes
flavipes. This document follows the new regulation.NOTE 2 Test results obtained according to earlier versions of this document and when the tests had started
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Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative against the Reticulitermes species of European termites. It allows
the determination of the concentration at which the product completely prevents attack by these insects
of impregnated wood of a susceptible species.This laboratory method provides one criterion by which the value of a product can be assessed. In making
this assessment the methods by which the preservative can be applied should be taken into account. It is
further recommended that results from this should be supplemented by those from other appropriate
tests, and above all by comparison with practical experience.When products which are very active at low concentrations are used it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other
products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use
of separate rooms, areas within rooms, extraction facilities and conditioning chambers as well as special
training for personnel (see also Annex C for environmental, health and safety precautions).
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1 Scope
This document specifies a method for the determination of the toxic values of a wood preservative against
the Reticulitermes species of European termites .This method is applicable to:
— water-insoluble chemicals which are being studied as active insecticides;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates; and— water-soluble materials, for example salts.
NOTE This method can be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics
of the total volume being sampled3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 PrincipleImpregnation of several sets of test specimens of susceptible wood with a series of solutions in which the
concentration of preservative is ranged in a given progression.Exposure of these test specimens to specified colonies of Reticulitermes and assessment of the attack
suffered after exposure under fixed conditions and over a fixed period. In providing biological validation
The method can be applied not only to different species of Reticulitermes, but also to other species of the family
of the Rhinotermitidae, adapting the conditions of temperature and humidity where necessary to the specific
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of individual species, it is essential that the locality of origin of each test termite species is given. The
description of the locality should at least include the district name.Comparison of these results with those obtained with untreated and solvent or diluent-treated control
test specimens.Derivation of the toxic values of the product under test.
5 Test materials
5.1 Biological material
Workers, soldiers and nymphs of an identified termite species of Reticulitermes.
The termite species and the locality of origin should be stated in the test report and their identification
should be proved.The termites should be obtained from colonies reared as described in Annex B.
5.2 Products and reagents
5.2.1 Substrate for establishing the colonies. A choice of:
5.2.1.1 Fine white quartz sand consisting of grains of crystallized silica, very pure (90 % silica), and
free from any organic substances5.2.1.2 A hydrated, laminar, aluminium-iron-magnesium silicate exfoliated to give particles of
3 31 mm to 3 mm with an apparent density of 80 kg/m to 90 kg/m
Particles of less than 1 mm shall be eliminated by sieving prior to use to ensure the absence of free water
and prevent any significant agglomeration of the particles.5.2.1.3 Rigid polyurethane foam with open pores
3 3 2
NOTE Horticulture foam with mass per unit of volume of 14 kg/m and compressive strength of 0,02 N/mm
to 0,03 N/mm is suitable. It is advisable to cut the foam into sheets 15 mm thick.
5.2.2 Fumigant (if necessary) xylene, technical grade, mixed isomers5.2.3 Water, complying with grade 3 of EN ISO 3696
5.2.4 Solvent or diluent, a suitable volatile liquid that will dissolve or dilute the preservative but does
not leave a residue in the wood which would have a toxic effect on the insect at the end of the conditioning
period5.2.5 Filter paper, ordinary quality, medium-fast grade
In France Fontainebleau sand, of which more than 97 % of the particles are between 75 µm and 300 µm in size,
provides these features.Determined in accordance with EN IS0 844.
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5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (26 ± 2) °C and at a minimum relative
humidity of (70 ± 5) %5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and relative humidity
(65 ± 5) %5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. It
is essential to follow safety procedures for handling flammable and toxic materials. Excessive exposure
of operators to solvents or their vapours should be avoided.5.3.4 Testing chamber, protected from light, ventilated and controlled at (26 ± 2) °C and at a minimum
relative humidity of (70 ± 5) %5.3.5 Treatment vessels, of a material that does not react with the preservative under test, for example
glass for organic products and polyethylene for salts containing fluorine5.3.6 Drying vessel(s), capable of holding sets of three test specimens (7.5), provided with a close-
fitting cover and containing supports that will give minimum contact with treated test specimens to be
placed on themThe vessels and supports shall be of a material that does not react with the preservative under test, for
example glass for organic compounds and polyethylene for products containing fluorine.
5.3.7 Weights, to provide ballast for the test specimensThe weights shall not react with any materials with which they come in contact during the test.
5.3.8 Safety equipment and protective clothing, appropriate for the test product and the test solvent,
to ensure the safety of the operator5.3.9 Vacuum vessels, fitted with stopcocks, capable of receiving the treatment
5.3.10 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa
5.3.11 Instruments, adapted for termite manipulation (aspirator, forceps)5.3.12 Test containers, suitable for holding the test specimens and of material resistant to the product
used, and fitted with perforated cover to provide a good exchange of air2 2
Base area
35 cm to 60 cm
Minimum height 8,5 cm
3 3
Volume
500 cm to 1 000 cm
5.3.13 Glass rings or rings of other inert materials, ca. 20 mm to ca. 25 mm high, ca. 15 mm to ca.
25 mm in diameter and with a wall thickness of ca. 1 mm to ca. 2 mm5.3.14 Protective gloves
The conditioning of test specimens after treatment can be carried out in the laboratory work area (5.3.3) provided
that this meets the conditions specified for the conditioning chamber (5.3.2).---------------------- Page: 9 ----------------------
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5.3.15 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of
0,01 g6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples should be stored
and handled in accordance with any written recommendations from the supplier.NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 will be used.
7 Test specimens7.1 Species of wood
The reference species is Scots pine (Pinus sylvestris Linnaeus).
Additional tests can be carried out using other species but, if so, this should be stated in the test report.
7.2 Wood qualityThe wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall
not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees
preferably felled in winter.NOTE Wood that has been kiln dried at temperatures below 60 °C can be used.
The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per
10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed
30 % of the whole.It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimensPrepare planed strips having a cross-section of (25 ± 0,5) mm × (15 ± 0,5) mm removing a minimum of
2 mm from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction
of the grain. The annual rings shall have a contact angle of greater than 10° to the broad faces of the test
specimens. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end grain
surfaces, to give test specimens (50 ± 0,5) mm long.The test specimens shall originate from a minimum of three trees or shall be taken at random from a
stock originally of more than 500 test specimens.7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2)
shall be (50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.For the purposes of calculating the mass of preservative retained per unit volume of wood (8.1.2.2) the
nominal volume of each test specimen shall be taken as 18,75 cmMark each test specimen so that it can be identified throughout the test.
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7.5 Number and distribution of test specimens
The test specimens shall be divided as follows:
a) treated test specimens: these are the test specimens which are impregnated and subject to attack by
Reticulitermes, use at least five test specimens for each concentration of the product;
b) untreated control test specimens for checking the virulence of the termite taken for the test: these
non-impregnated test specimens are subjected to attack by Reticulitermes; they are three in number;
c) solvent or diluent treated control test specimens subjected to attack by Reticulitermes; they are three
in number.8 Procedure
8.1 Preparation of test specimens
8.1.1 Conditioning of test specimens prior to treatment
Allow the test specimens to condition in the conditioning chamber (5.3.2) for a minimum of two weeks.
8.1.2 Treatment of test specimens8.1.2.1 Preparation fo treatment solutions
8.1.2.1.1 Solid preservatives
Water-soluble preservatives: dissolve the preservative in water (5.2.3) to the required concentration.
Non-water-soluble preservatives: dissolve the preservative in an appropriate solvent (5.2.4) to the
required concentration.8.1.2.1.2 Liquid preservatives
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is
a concentrate, dilute the preservative with the diluent to the required working concentration, using the
procedure specified by the supplier.Prepare a series of at least five concentrations by mass, distributed evenly about the expected toxic
values. A solvent or diluent control, i.e. treatment at concentration = 0, shall also be used. If the
approximate toxic values are unknown, the concentrations shall form a widely spaced geometric
progression for a first test and a more closely spaced geometric or arithmetic progression for subsequent
tests.All treatment solutions shall be freshly prepared.
8.1.2.2 Impregnation
Carry out impregnation in ascending order of concentration, starting with the solvent control
(concentration = 0). The following procedure ensures the required complete impregnation of test
specimens by the test solutions. For each concentration weigh each test specimen to the nearest 0,05 g,
and then stack the test specimens in one of the treatment vessels (5.3.5) so that as much of their face as
possible is exposed (e.g. by piling them crosswise). Ballast the stack of test specimens with weights (5.3.7)
to prevent them from floating later when the liquid is admitted.Place each treatment vessel in one of the vacuum vessels (5.3.9) and reduce the pressure to 700 Pa.
Maintain this for 15 min. Observe the proper safety measures for vacuum vessels. After this period, close
the stopcock to the vacuum pump (5.3.10) and open the other stopcock to allow the solution of
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preservative to be drawn into the treatment vessel. Keep the test specimens covered completely by the
solution throughout the remainder of the impregnation process.Next, admit air to bring the vacuum vessel back to atmospheric pressure, remove the treatment vessel
with its submerged test specimens from the vacuum vessel, cover it and leave it for 2 h, adding further
solution if necessary to keep the test specimens fully covered by the liquid.After this impregnation treatment remove the test specimens one by one, remove the excess liquid from
their faces by lightly blotting with filter paper (5.2.5), and immediately weigh to the nearest 0,05 g.
In the case of water-soluble preservatives, for example salts and organic chemicals which are being
studied as active substances, calculate the mass of active matter retained by each test specimen from the
mass of solution absorbed and its concentration. When dealing with preservative formulations whose
constituents can be selectively absorbed by wood, it is necessary to carry out chemical analysis of the
solution before and after impregnation. Similarly, analysis is also recommended if very dilute solutions
are used.In the case of organic formulations and organic water-dispersible formulations the retention is expressed
for each test specimen in terms of the corresponding mass of the formulation retained but, if a
concentration is supplied, the retention is expressed in terms of the solution prepared ready for use as
specified by the supplier.Calculate the mass of preservative retained per unit volume of wood in kilograms per cubic metre, for
each test specimen.8.1.3 Drying and conditioning of the test specimens after treatment
Drying and conditioning of the test specimens depend on the nature of the product under test and on the
solvent or diluent used. It can be necessary to modify the conditioning procedure but, if so, this should be
stated in the test report.Arrange the impregnated test specimens treated with each preservative concentration on their narrow
faces, resting on two glass rods, not touching each other in the drying vessel (5.3.6). Place the cover on
the drying vessel. Place the drying vessel in the conditioning chamber (5.3.2). Invert the test specimens
twice each week during the subsequent drying period, temporarily removing the cover to perform these
operations.To prevent mould growth on test specimens treated with water-diluted preservatives, place a small dish
containing the xylene (5.2.2) in the drying vessel (5.3.6).During the first two weeks retain the cover on the drying vessel.
During the third week uncover the drying vessel progressively each day to allow the test specimens to
dry steadily.From the beginning of the fourth week leave the drying vessel fully open. Drying shall be complete at the
end of the fourth week.In the same way, place the test specimens impregnated with water-insoluble preservatives in the drying
vessel for one week and then open it gradually throughout the second week. From the beginning of the
third week leave the vessel fully open.If, in case of slow drying products, the conditioning period is extended, the extended conditioning periods
shall be stated in the test report.8.2 Exposure of the test specimens to the insects
8.2.1 Collecting and selecting the termites
Pick up the insects individually using the instrument (5.3.11). Make up groups of 250 workers, rejecting
insects which are moulting (indicated by the dull white colour of the abdomen) also those which appear
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to be wounded or remain motionless. To each group made up in this way add a number of soldiers
corresponding to the proportion found in the colony from which the workers were taken: add a
corresponding proportion of nymphs (1 % to 5 %).The number of colonies to be prepared as indicated above is equal to the number of test specimens to be
subjected to attack by the termites.If the required number of termites is more than that in a single culture, the control series and test series
shall contain the same number of groups from each colony. Termites from different colonies shall not be
mixed in a single group.8.2.2 Instalaltion of the termites
8.2.2.1 With sand
In a separate test container (5.3.12), remoisten the adequate quantity of sand for the test by introducing
first the water (5.2.3) and then the sand (5.2.1.1) in the proportions of one volume of water to four
volumes of sand.In each test container (5.3.12) form a layer of remoistened, non-compacted sand 40 mm to 60 mm thick.
At the (approximate) centre of the test container, place some wood from the original culture
(approximately 0,5 g) and push it down to the bottom of the test container.In each test container, place a glass ring (5.3.13) against one of the vertical walls of the test container and
in the middle of this wall; place it in the substrate so that it just protrudes the surface. Distribute a group
of termites made up as indicated in 8.2.1 in each test container, spreading them carefully over the entire
substrate (see Figure 1).Close each test container by means of its lid and place it in the testing chamber (5.3.4).
8.2.2.2 With aluminium-iron-magnesium silicatePrepare enough a
...
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