Nanomanufacturing - Key control characteristics - Part 3-3: Luminescent nanomaterials - Determination of fluorescence lifetime of semiconductor quantum dots using time correlated single photon counting (TCSPC)

IEC 62607:2020, a Technical Specification, provides a method for determining the fluorescence lifetime of semiconductor quantum dots (QDs) using the time correlated single photon counting (TCSPC) technique. TCSPC is suitable for testing fluorescence lifetime in the range from picoseconds to nanoseconds. This document is only applicable to liquid samples that are stable dispersions of QDs. It is not applicable to solid samples.
This document includes:
– outlines of the experimental procedures,
– data processing, and
– case study.

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IEC TS 62607-3-3:2020 - Nanomanufacturing - Key control characteristics - Part 3-3: Luminescent nanomaterials - Determination of fluorescence lifetime of semiconductor quantum dots using time correlated single photon counting (TCSPC)
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IEC TS 62607-3-3 ®
Edition 1.0 2020-09
Nanomanufacturing – Key control characteristics –
Part 3-3: Luminescent nanomaterials – Determination of fluorescence lifetime of
semiconductor quantum dots using time correlated single photon counting
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IEC TS 62607-3-3 ®
Edition 1.0 2020-09
Nanomanufacturing – Key control characteristics –

Part 3-3: Luminescent nanomaterials – Determination of fluorescence lifetime of

semiconductor quantum dots using time correlated single photon counting

ICS 07.030, ICS 07.120 ISBN 978-2-8322-8881-8

– 2 – IEC TS 62607-3-3:2020  IEC 2020
1 Scope . 6
2 Normative references . 6
3 Terms, definitions, and abbreviated terms . 6
3.1 Terms and definitions . 6
3.2 Abbreviated terms . 7
4 Test principle . 7
5 Sample preparation . 8
6 Measurement . 8
6.1 TCSPC fluorescence spectrometer . 8
6.2 Measurement procedure . 8
6.2.1 Instrument preparation . 8
6.2.2 Fluorescence lifetime decay curve measurement . 9
6.2.3 IRF measurement . 10
6.2.4 Data analysis . 10
7 Test reports . 10
8 Uncertainty source. 10
Annex A (informative) Case study for determining fluorescence lifetime of
semiconductor quantum dots . 11
A.1 General . 11
A.2 QDs sample . 11
A.3 Instruments . 11
A.4 Measurement conditions for TCSPC . 11
A.5 Procedures for measurement . 11
A.5.1 Instrument preparation . 11
A.5.2 Fluorescence lifetime decay curve measurement . 11
A.5.3 IRF measurement . 12
A.6 Results of fluorescence lifetime decay curve . 12
A.7 Data analysis . 13
Annex B (informative) Typical laser input excitation sources used in TCSPC . 14
Bibliography . 15

Figure 1 – The schematic of start-stop times in time-resolved fluorescence
measurement with TCSPC . 7
Figure 2 – The working schematic of TCSPC fluorescence spectrometer . 8
Figure 3 – Examples for typical single exponential decay curves obtained in different
measurement ranges . 9
Figure A.1 – Typical fluorescence lifetime decay curve . 12
Figure A.2 – Fitting result curve and IRF curve . 13

Table B.1 – Typical laser input excitation sources used in TCSPC . 14
Table B.2 – Fluorescence lifetime of commonly used semiconductor QDs . 14


Part 3-3: Luminescent nanomaterials – Determination of fluorescence
lifetime of semiconductor quantum dots using time correlated single
photon counting (TCSPC)
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IEC TS 62607-3-3, which is a Technical Specification, has been prepared by IEC technical
committee 113: Nanotechnology standardization for electrotechnical products and systems.

– 4 – IEC TS 62607-3-3:2020  IEC 2020
The text of this Technical Specification is based on the following documents:
Enquiry draft Report on voting
113/490/DTS 113/529/RVDTS
Full information on the voting for the approval of this Technical Specification can be found in
the report on voting indicated in the above table.
This document has been drafted in accordance with the ISO/IEC Directives, Part 2.
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Key control characteristics, can be found on the IEC website.
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Fluorescence lifetime is considered as the average time that luminescent materials spend in
the excited state before emitting a photon and returning to the ground state. Fluorescence
lifetime can vary widely from picoseconds to hundreds of nanoseconds, even to microseconds
or milliseconds, depending on the type of luminescent nanomaterials.
Fluorescence lifetime is an important property of luminescent materials. Fluorescence lifetime
does not depend on fluorophore concentration, absorption by the sample, thickness of the
sample, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation
intensity. It is affected by external factors, such as t

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