ISO/FDIS 25353

• Abstract:

  This document specifies a qualitative procedure to qualifying leather animal species of (fibrous) protein from leather by MALDI-TOF mass spectrometer (MS). The composition of other fibres can be measured by methods described in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex C as an example of mixtures of (fibrous) protein from leather and polyester). The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis of their morphology, according to ISO/TR 11827.

• Status: Not Published
• ICS: 59.060.01 - Textile fibres in general
• Technical Committee: ISO/TC 38 - Textiles
• Drafting Committee: ISO/TC 38/WG 22 - Composition and chemical testing
• Current Stage: 5020 - FDIS ballot initiated: 2 months. Proof sent to secretariat
• Start Date: 26-Aug-2026
• Completion Date: 26-Aug-2026

• Consolidates: FprEN ISO 25353 - Textiles - Qualitative proteomic analysis of protein fibres from leather - Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (ISO/FDIS 25353:2026)
• Effective Date: 12-Feb-2026
• Consolidates: ISO/IEC 30118-14:2021 - Information technology – Open Connectivity Foundation (OCF) Specification — Part 14: OCF resource to BLE mapping specification
• Effective Date: 21-Jun-2025

Overview

ISO/FDIS 25353: Textiles - Qualitative proteomic analysis of protein fibres from leather - Peptide detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides an international method for the qualitative identification of animal species in leather-derived protein fibres. By utilizing MALDI-TOF mass spectrometry, this standard enables precise discrimination of animal species even in cases where traditional microscopy techniques prove insufficient due to the processed nature of the leather fibres.

This document is developed by ISO/TC 38, Textiles, and is intended for industry professionals involved in the quality assurance, sourcing, and verification of leather textiles and leather-spun yarn products.

Key Topics

• Animal Species Identification: The standard describes how to extract and enzymatically digest proteins from leather fibres, followed by mass spectrometric analysis to identify animal-specific peptide markers.
• Use of MALDI-TOF MS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is detailed for detecting species-specific peptides, ensuring high specificity in identification.
• Complementary Analysis Methods: The procedure complements fibre composition analysis governed by the ISO 1833 series, combining results to give a comprehensive view of blended textile compositions.
• Specimen Preparation: Guidelines for preparing test samples from leather, including cutting, drying, and weighing, help ensure standardized results and reproducibility.
• Reporting Requirements: The standard outlines the essential information that must be included in test reports, supporting traceability and transparency.

Applications

Implementing ISO/FDIS 25353 offers significant benefits to a range of stakeholders:

• Leather Goods Manufacturing: Ensures material authenticity and supports labeling claims regarding animal origin, which is critical for quality and regulatory compliance.
• Textile Quality Control: Enables laboratories and textile processors to accurately determine the composition of leather blends, supporting product development and market differentiation.
• Consumer Protection: Facilitates transparent product information, allowing consumers to make informed purchasing decisions, particularly where ethical or species-specific criteria are important.
• Sustainability and Traceability: Assists in the verification of recycled or reclaimed leather materials, contributing to responsible sourcing and sustainable manufacturing.
• Forensic and Regulatory Use: Equips authorities and independent inspectors with a scientific method for animal species authentication in disputed cases or when provenance is critical.

Related Standards

Organizations applying ISO/FDIS 25353 may also reference key related international textile and leather standards:

• ISO 17131: Leather - Identification of leather with microscopy. Describes classical microscopic techniques for leather identification.
• ISO/TR 11827: Textiles - Composition testing - Identification of fibres. Provides guidance on fibre morphology identification.
• ISO 1833 Series: Textiles - Quantitative chemical analysis. Covers methods for determining the composition of blends, such as polyester and protein fibres.
• ISO 20418-2: Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF MS. Offers similar mass spectrometry procedures for other animal fibres.

Practical Value

Adopting ISO/FDIS 25353 enhances the reliability, repeatability, and international comparability of animal species identification in leather textiles. The use of MALDI-TOF MS addresses the microscopic method's limitations, especially for highly processed or blended samples. This standard is particularly relevant for manufacturers, laboratories, importers, and brands seeking robust compliance and quality verification methods in the global textile and leather markets.

FINAL DRAFT
International
Standard
ISO/TC 38
Textiles — Qualitative proteomic
Secretariat: SAC
analysis of protein fibres from
Voting begins on:
leather — Peptide detection using
2026-08-26
matrix-assisted laser desorption/
Voting terminates on:
ionization time-of-flight mass
2026-10-21
spectrometry (MALDI-TOF MS)
Textiles — Analyse protéomique qualitative de fibres protéiques
issues du cuir — Détection des peptides par MALDI-TOF MS
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
FINAL DRAFT
International
Standard
ISO/TC 38
Textiles — Qualitative proteomic
Secretariat: SAC
analysis of protein fibres from
Voting begins on:
leather — Peptide detection using
matrix-assisted laser desorption/
Voting terminates on:
ionization time-of-flight mass
spectrometry (MALDI-TOF MS)
Textiles — Analyse protéomique qualitative de fibres protéiques
issues du cuir — Détection des peptides par MALDI-TOF MS
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland Reference number
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms. 2
5 Principle . 2
6 Reagents . 2
7 Apparatus . 3
8 Preparation of test specimens . 3
9 Procedure . 4
9.1 Protein extraction .4
9.2 Animal species identification of protein fibres from leather .4
10 Test report . 4
Annex A (normative) Animal species-specific marker in MALDI-TOF-MS analysis . 5
Annex B (informative) Animal species-specific peaks in MALDI-TOF-MS analysis. 6
Annex C (informative) Quantitative analysis of mixtures of protein fibres from leather and
polyester .13
Bibliography . 14

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles, in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 248, Textiles and textile products, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
Introduction
Protein fibres from leather are staple fibres obtained by grinding leather scrap or shaving dust. A leather
spun yarn can be produced by spinning these leather fibres with other fibres.
In the protein fibres from leather industries, the identification of animal species is very important to
guarantee the quality of leather products. However, it is difficult to apply the microscopy method (ISO 17131
[1]
) to identify the protein fibres from leather, since it is not able to observe the cross-section structure of
[1]
leather or its surface. In the case of ISO 17131 , it is necessary to compare the material structure with
typical pictures of known samples by means of microscopy. For that reason, it can be difficult to absolutely
determine animal species of protein fibres from leather.
In order to overcome the difficulties of the microscope method, a method of applying matrix-assisted laser
desorption ionization-time of flight mass spectrometry mass spectrometer (MALDI-TOF MS) has been
developed for the discrimination of animal species of protein fibres from leather.
Since leather is mostly composed of protein, it utilizes enzymes to break down proteins into peptides and
analyse their mass spectra.
The method solves the problem of difficulty in identifying species when leather is pulverized and used, and
can be seen as an advanced technology in that it can be applied to various species as well as cowhide, which
is widely used.
v
FINAL DRAFT International Standard ISO/FDIS 25353:2026(en)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF MS)
1 Scope
This document specifies a qualitative procedure for identifying the animal species of leather using protein
fibres extracted from leather using matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS).
This document does not cover the composition of other fibres. This can be measured by methods described
in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see
Annex C as an example of mixtures of protein fibres from leather and poly
...

ISO/DISFDIS 25353
ISO/TC 38
Secretariat: SAC
Date: 2026-07-2208-12
Textiles — Qualitative proteomic analysis of protein fibres from
leather — Peptide detection using MALDI-TOF MSmatrix-assisted
laser desorption/ionization time-of-flight mass spectrometry
(MALDI-TOF MS)
DISTextiles — Analyse protéomique qualitative de fibres protéiques issues du cuir — Détection des peptides par
MALDI-TOF MS
FDIS stage
TTThhhiiisss d d drrraftaftaft i i isss s s suuubbbmmmiiittttttededed t t tooo  a pa pa parararallel vallel vallel vooottte e e iiinnn I I ISSSOOO,,, C C CEEENNN.
Voting begins on: 2025-09-25
Voting terminates on: 2025-12-18

All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: [e-mail]
Website: www.iso.org
Published in Switzerland
ii
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols and abbreviated terms . 2
5 Principle . 2
6 Reagents . 2
7 Apparatus . 3
8 Preparation of test specimens . 4
9 Procedure . 4
9.1 Protein extraction . 4
9.2 Animal species identification of protein fibres from leather . 4
10 Test report . 4
Annex A (normative) Animal species-specific marker in MALDI-TOF-MS analysis . 6
Annex B (informative) Animal species-specific peaks in MALDI-TOF-MS analysis . 7
Annex C (informative) Quantitative analysis of mixtures of protein fibres from leather and
polyester . 14
Bibliography . 15

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that this
may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles, in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 248, Textiles and textile products, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Protein fibres from leather are staple fibres obtained by grinding leather scrap or shaving dust. A leather spun
yarn can be produced by spinning these leather fibres with other fibres.
In the protein fibres from leather industries, the identification of animal species is very important to guarantee
[1]
the quality of leather products. However, it is difficult to apply the microscopy method (ISO 17131 (()) to
identify the protein fibres from leather, since it is not able to observe the cross-section structure of leather or
[1]
its surface. In the case of ISO 17131 , it is necessary to compare the material structure with typical pictures
of known samples by means of microscopy. For that reason, it can be difficult to absolutely determine animal
species of protein fibres from leather.
In order to overcome the difficulties of the microscope method, a method of applying matrix-assisted laser
desorption ionization-time of flight mass spectrometry mass spectrometer (MALDI-TOF MS) has been
developed for the discrimination of animal species of protein fibres from leather.
Since leather is mostly composed of protein, it utilizes enzymes to break down proteins into peptides and
analyse their mass spectra.
The method solves the problem of difficulty in identifying species when leather is pulverized and used, and
can be seen as an advanced technology in that it can be applied to various species as well as cowhide, which is
widely used.
v
Textiles — Qualitative proteomic analysis of protein fibres from
leather — Peptide detection using MALDI-TOF MSmatrix-assisted
laser desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
1 Scope
This document specifies a qualitative procedure for identifying the animal species of leather using protein
fibres extracted from leather using matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS).
This document does not cover the composition of other fibres. This can be measured by methods described in
the ISO 1833 series. Both results are then combined to determine the whole composition of fibres (see Annex
C as an example of mixtures of protein fibres from leather and polyester).
The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis
[2]
of their morphology, according to ISO/TR 11827 ISO/TR 11827.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 4044, Leather — Chemical tests — Preparation of chemical test samples
ISO 20418-2:2018, Textiles — Qualitative and quantitative proteomic analysis of some animal hair fibres — Part
2: Peptide detection using MALDI-TOF MS
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at http://www.iso.org/obphttp://www.iso.org/obp
— IEC Electropedia: available at http://www.electropedia.org/
3.1
protein
polymers of amino acids that play many critical roles in the body
[3]
[SOURCE: ISO 20418-2:2018 ISO 20418-2:2018, 3.2]
3.2
peptide
small protein (3.1) consisting of approximately less than 50 amino acids
[3]
[SOURCE: ISO 20418-2:2018 ISO 20418-2:2018,, 3.3]
3.3
marker
mass to change ratio of animal species specific monoisotopic peak used for identificationNote 1 to entry: See
Clause 4 for an explanation of mass to change retion.identification
N
...

PROJET FINAL
Norme
internationale
ISO/TC 38
Textiles — Analyse protéomique
Secrétariat: SAC
qualitative de fibres protéiques
Début de vote:
issues du cuir — Détection des
2026-08-26
peptides par spectrométrie
Vote clos le:
de masse à temps de vol avec
2026-10-21
ionisation/désorption laser assistée
par matrice (MALDI-TOF MS)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
LES DESTINATAIRES DU PRÉSENT PROJET SONT
INVITÉS À PRÉSENTER, AVEC LEURS OBSERVATIONS,
NOTIFICATION DES DROITS DE PROPRIÉTÉ DONT ILS
AURAIENT ÉVENTUELLEMENT CONNAISSANCE ET À
FOURNIR UNE DOCUMENTATION EXPLICATIVE.
OUTRE LE FAIT D’ÊTRE EXAMINÉS POUR
ÉTABLIR S’ILS SONT ACCEPTABLES À DES FINS
INDUSTRIELLES, TECHNOLOGIQUES ET COM-MERCIALES,
AINSI QUE DU POINT DE VUE DES UTILISATEURS, LES
PROJETS DE NORMES
TRAITEMENT PARALLÈLE ISO/CEN
INTERNATIONALES DOIVENT PARFOIS ÊTRE CONSIDÉRÉS
DU POINT DE VUE DE LEUR POSSI BILITÉ DE DEVENIR DES
NORMES POUVANT
SERVIR DE RÉFÉRENCE DANS LA RÉGLEMENTATION
NATIONALE.
Numéro de référence
PROJET FINAL
Norme
internationale
ISO/TC 38
Textiles — Analyse protéomique
Secrétariat: SAC
qualitative de fibres protéiques
Début de vote:
issues du cuir — Détection des
2026-08-26
peptides par spectrométrie
Vote clos le:
de masse à temps de vol avec
2026-10-21
ionisation/désorption laser assistée
par matrice (MALDI-TOF MS)
Textiles — Qualitative proteomic analysis of protein fibres
from leather — Peptide detection using matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry (MALDI-
TOF MS)
LES DESTINATAIRES DU PRÉSENT PROJET SONT
INVITÉS À PRÉSENTER, AVEC LEURS OBSERVATIONS,
NOTIFICATION DES DROITS DE PROPRIÉTÉ DONT ILS
AURAIENT ÉVENTUELLEMENT CONNAISSANCE ET À
FOURNIR UNE DOCUMENTATION EXPLICATIVE.
DOCUMENT PROTÉGÉ PAR COPYRIGHT
OUTRE LE FAIT D’ÊTRE EXAMINÉS POUR
ÉTABLIR S’ILS SONT ACCEPTABLES À DES FINS
© ISO 2026 INDUSTRIELLES, TECHNOLOGIQUES ET COM-MERCIALES,
AINSI QUE DU POINT DE VUE DES UTILISATEURS, LES
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
PROJETS DE NORMES
TRAITEMENT PARALLÈLE ISO/CEN
INTERNATIONALES DOIVENT PARFOIS ÊTRE CONSIDÉRÉS
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
DU POINT DE VUE DE LEUR POSSI BILITÉ DE DEVENIR DES
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
NORMES POUVANT
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
SERVIR DE RÉFÉRENCE DANS LA RÉGLEMENTATION
NATIONALE.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse Numéro de référence
ii
Sommaire Page
Avant-propos .iv
Introduction .v
1 Domaine d'application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Symboles et abréviations . 2
5 Principe. 2
6 Réactifs . 2
7 Appareillage . 3
8 Préparation des éprouvettes . 3
9 Mode opératoire . 4
9.1 Extraction des protéines .4
9.2 Identification des espèces animales à l'origine des fibres protéiques issues du cuir .4
10 Rapport d'essai . 4
Annexe A (normative) Marqueur spécifique de l'espèce animale dans l'analyse par MALDI-
TOF MS . 5
Annexe B (informative) Pics spécifiques des espèces animales dans l'analyse par MALDI-TOF MS . 6
Annexe C (informative) Analyse quantitative des mélanges de fibres protéiques issues du cuir
avec des fibres de polyester .13
Bibliographie . 14

iii
Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale d'organismes nationaux
de normalisation (comités membres de l'ISO). L'élaboration des Normes internationales est en général
confiée aux comités techniques de l'ISO. Chaque comité membre intéressé par une étude a le droit de faire
partie du comité technique créé à cet effet. Les organisations internationales, gouvernementales et non
gouvernementales, en liaison avec l'ISO participent également aux travaux. L'ISO collabore étroitement avec
la Commission électrotechnique internationale (IEC) en ce qui concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier, de prendre note des différents
critères d'approbation requis pour les différents types de documents ISO. Le présent document a
été rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir
www.iso.org/directives).
L'ISO attire l'attention sur le fait que la mise en application du présent document peut entraîner l'utilisation
d'un ou de plusieurs brevets. L'ISO ne prend pas position quant à la preuve, à la validité et à l'applicabilité de
tout droit de propriété revendiqué à cet égard. À la date de publication du présent document, l'ISO n'avait pas
reçu notification qu'un ou plusieurs brevets pouvaient être nécessaires à sa mise en application. Toutefois,
il y a lieu d'avertir les responsables de la mise en application du présent document que des informations
plus récentes sont susceptibles de figurer dans la base de données de brevets, disponible à l'adresse
www.iso.org/brevets. L'ISO ne saurait être tenue pour responsable de ne pas avoir identifié de tels droits de
brevet.
Les appellations commerciales éventuellement mentionnées dans le présent document sont données pour
information, par souci de commodité, à l'intention des utilisateurs et ne sauraient constituer un engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l'ISO liés à l'évaluation de la conformité, ou pour toute information au sujet de l'adhésion de
l'ISO aux principes de l'Organisation mondiale du commerce (OMC) concernant les obstacles techniques au
commerce (OTC), voir le lien suivant: www.iso.org/avant-propos.
Le présent document a été élaboré par le comité technique ISO/TC 38, Textiles, en collaboration avec le
comité technique CEN/TC 248, Textiles et produits textiles, du Comité européen de normalisation (CEN)
conformément à l'Accord de coopération technique entre l'ISO et le CEN (Accord de Vienne).
Il convient que l'utilisateur adresse tout retour d'information ou toute question concernant le présent
document à l'organisme national de normalisation de son pays. Une liste exhaustive desdits organismes se
trouve à l'adresse www.iso.org/members.html.

iv
Introduction
Les fibres protéiques issues du cuir sont des fibres discontinues obtenues par broyage de chutes de cuir ou
de résidus de rasage. Un filé de fibres issues du cuir peut être produit en filant ces fibres issues du cuir avec
d'autres fibres.
Dans les fibres protéiques provenant de l'industrie du cuir, l'identification des espèces animales est très
importante pour garantir la qualité des produits en cuir. Cependant, il est difficile d'appliquer la méthode
[1]
de microscopie (ISO 17131 ) à l'identification des fibres protéiques issues du cuir, car cette méthode ne
[1]
permet pas d'observer la structure de la section du cuir ni sa surface. Dans le cas de l'ISO 17131, il est
nécessaire de comparer par microscopie la structure du matériau avec des images types d'échantillons
connus. Pour cette raison, il peut être difficile de déterminer avec certitude les espèces animales à l'origine
des fibres protéiques issues du cuir.
Pour remédier aux lacunes de la méthode au microscope, une méthode utilisant la spectrométrie de masse
à temps de vol avec ionisation/désorption laser assistée par matrice (MALDI-TOF MS) au moyen d'un
spectromètre de masse a été élaborée pour discriminer les espèces animales à l'origine des fibres protéiques
issues du cuir.
Le cuir étant principalement composé de protéines, ladite méthode utilise des enzymes pour décomposer les
protéines en peptides et analyser leur spectre de masse.
Cette méthode résout les difficultés à identifier les espèces lorsque le cuir est pulvérisé et usagé. Elle peut
être considérée comme une technologie avancée en ce sens qu'elle peut s'appliquer à diverses espèces, ainsi
qu'au cuir de vache qui est largement utilisé.

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PROJET FINAL Norme internationale ISO/FDIS 25353:2026(fr)
Textiles — Analyse protéomique qualitative de fibres
protéiques issues du cuir — Détection des peptides par
spectrométrie de masse à temps de vol avec ionisation/
désorption laser assistée par matrice (MALDI-TOF MS)
1 Domaine d'application
Le présent document spécifie un mode opératoire qualitatif pour identifier à l'aide de fibres protéiques
issues du cuir les espèces animales utilisées pour le cuir, par spectrométrie de masse à temps de vol avec
ionisation/désorption laser assistée par matrice (MALDI-TOF MS).
Le présent document ne couvre pas la composition des autres fibres. Elle peut être mesurée par les méthodes
décrites dans la série ISO 1833. Les deux résultats sont ensuite combinés pour déterminer la composition
totale des fibres (voir Annexe C pour un exemple de mélanges de fibres protéiques issues du cuir et de
polyester).
La méthode repose sur une identification préliminaire, par microscopie optique, de toutes les fibres
[2]
contenues dans un mélange sur la base de leur morphologie, conformément à l'ISO/TR 11827 .
2 Réfé
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