ISO 21703:2019
(Main)Surface active agents — Microbiology — Microbiological test methods for liquid hand dishwashing
Surface active agents — Microbiology — Microbiological test methods for liquid hand dishwashing
This document provides microbiological test methods for enumeration and detection of aerobic mesophilic bacteria, detection of Escherichia coli and Pseudomonas aeruginosa in liquid hand dishwashing.
Agents de surface — Microbiologie — Méthodes d’essai microbiologique pour les détergents liquides de lavage de vaisselle à la main
General Information
Buy Standard
Standards Content (Sample)
INTERNATIONAL ISO
STANDARD 21703
First edition
2019-01
Surface active agents — Microbiology
— Microbiological test methods for
liquid hand dishwashing
Agents de surface — Microbiologie — Méthodes d’essai
microbiologique pour les détergents liquides de lavage de vaisselle
à la main
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Diluents and culture media . 2
5.1 General . 2
5.2 Neutralizing diluent and diluents . 2
5.2.1 General. 2
5.2.2 Neutralizing diluent: Fluid casein digest — soy lecithin — polysorbate 20
medium (SCDLP 20 broth) . 3
5.2.3 Diluent for the bacterial suspension (Tryptone sodium chloride solution) . 3
5.3 Culture medium for counting . 3
5.3.1 General. 3
5.3.2 Soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA) . 3
5.3.3 Other medium for counting . 4
5.4 Culture medium for detection . 4
5.4.1 General. 4
5.4.2 Enrichment broth: Eugon LT 100 broth . 5
5.5 Culture media for isolation and identification . 5
5.5.1 MacConkey agar medium . 5
5.5.2 Levine eosin-methylene blue agar medium (EMB agar medium) . 6
5.5.3 Cetrimide agar medium . 6
5.5.4 Pseudomonas agar medium for detection of pyocyanin (Pseudomonas agar P) . 7
6 Apparatus and glassware . 7
7 Strains of microorganisms . 7
8 Handling of the product and laboratory samples . 8
9 Procedure. 8
9.1 General recommendation . 8
9.2 Preparation of the initial suspension . 8
9.3 Enumeration methods . 8
9.3.1 Dilutions for enumeration . 8
9.3.2 Plate-count methods . 8
10 Method for detection and identification .11
10.1 Enrichment .11
10.2 Isolation .11
10.3 Detection of aerobic mesophilic bacteria .11
10.4 Detection and Identification of Escherichia coli .11
10.4.1 General.11
10.4.2 Gram’s stain .11
10.4.3 Culture on Levine eosin-methylene blue agar medium (EMB agar medium) .11
10.4.4 Expression of results .12
10.5 Detection and identification of Pseudomonas aeruginosa .12
10.5.1 General.12
10.5.2 Gram’s stain .12
10.5.3 Oxidase test .12
10.5.4 Culture on Pseudomonas agar medium for detection of pyocyanin .12
10.5.5 Expression of results .12
11 Neutralization of the antimicrobial properties of the product .13
11.1 General .13
11.2 Preparation of inoculum .13
11.3 Suitability of counting methods .13
11.3.1 Principle .13
11.3.2 Suitability test of the pour-plate method .13
11.3.3 Suitability of the surface spread method .13
11.3.4 Suitability of the membrane filtration method .14
11.4 Suitability of the detection method .14
11.4.1 Procedure .14
11.4.2 Interpretation .14
12 Test report .15
Annex A (informative) Neutralizers of antimicrobial activity of preservatives and rinsing
liquids .16
Annex B (informative) Other culture media .17
Bibliography .19
iv © ISO 2019 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 91, Surface active agents.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
INTERNATIONAL STANDARD ISO 217
...
INTERNATIONAL ISO
STANDARD 21703
First edition
2019-01
Surface active agents — Microbiology
— Microbiological test methods for
liquid hand dishwashing
Agents de surface — Microbiologie — Méthodes d’essai
microbiologique pour les détergents liquides de lavage de vaisselle
à la main
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Diluents and culture media . 2
5.1 General . 2
5.2 Neutralizing diluent and diluents . 2
5.2.1 General. 2
5.2.2 Neutralizing diluent: Fluid casein digest — soy lecithin — polysorbate 20
medium (SCDLP 20 broth) . 3
5.2.3 Diluent for the bacterial suspension (Tryptone sodium chloride solution) . 3
5.3 Culture medium for counting . 3
5.3.1 General. 3
5.3.2 Soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA) . 3
5.3.3 Other medium for counting . 4
5.4 Culture medium for detection . 4
5.4.1 General. 4
5.4.2 Enrichment broth: Eugon LT 100 broth . 5
5.5 Culture media for isolation and identification . 5
5.5.1 MacConkey agar medium . 5
5.5.2 Levine eosin-methylene blue agar medium (EMB agar medium) . 6
5.5.3 Cetrimide agar medium . 6
5.5.4 Pseudomonas agar medium for detection of pyocyanin (Pseudomonas agar P) . 7
6 Apparatus and glassware . 7
7 Strains of microorganisms . 7
8 Handling of the product and laboratory samples . 8
9 Procedure. 8
9.1 General recommendation . 8
9.2 Preparation of the initial suspension . 8
9.3 Enumeration methods . 8
9.3.1 Dilutions for enumeration . 8
9.3.2 Plate-count methods . 8
10 Method for detection and identification .11
10.1 Enrichment .11
10.2 Isolation .11
10.3 Detection of aerobic mesophilic bacteria .11
10.4 Detection and Identification of Escherichia coli .11
10.4.1 General.11
10.4.2 Gram’s stain .11
10.4.3 Culture on Levine eosin-methylene blue agar medium (EMB agar medium) .11
10.4.4 Expression of results .12
10.5 Detection and identification of Pseudomonas aeruginosa .12
10.5.1 General.12
10.5.2 Gram’s stain .12
10.5.3 Oxidase test .12
10.5.4 Culture on Pseudomonas agar medium for detection of pyocyanin .12
10.5.5 Expression of results .12
11 Neutralization of the antimicrobial properties of the product .13
11.1 General .13
11.2 Preparation of inoculum .13
11.3 Suitability of counting methods .13
11.3.1 Principle .13
11.3.2 Suitability test of the pour-plate method .13
11.3.3 Suitability of the surface spread method .13
11.3.4 Suitability of the membrane filtration method .14
11.4 Suitability of the detection method .14
11.4.1 Procedure .14
11.4.2 Interpretation .14
12 Test report .15
Annex A (informative) Neutralizers of antimicrobial activity of preservatives and rinsing
liquids .16
Annex B (informative) Other culture media .17
Bibliography .19
iv © ISO 2019 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 91, Surface active agents.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
INTERNATIONAL STANDARD ISO 217
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.