ISO 16000-39:2019
(Main)Indoor air — Part 39: Determination of amines — Analysis of amines by (ultra-) high-performance liquid chromatography coupled to high resolution or tandem mass spectrometry
Indoor air — Part 39: Determination of amines — Analysis of amines by (ultra-) high-performance liquid chromatography coupled to high resolution or tandem mass spectrometry
This document, along with ISO 16000-38, specifies the measurement method for determining the mass concentration of primary, secondary and tertiary aliphatic and aromatic amines in indoor air using accumulated sampling and high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS-MS) or high-resolution mass spectrometry (HRMS). The analytical procedure is covered by this document. The sampling procedure and the manufacturing of the samplers are covered by ISO 16000-38. This document describes specifications for the chromatography and the mass spectroscopy for the amines. Measurement results are expressed in µg/m3. Although primarily intended for the measurement of amines listed in Tables A.1 and A.2, it can also be used for the measurement of other amines in indoor air. This document gives instructions and describes procedures for the inclusion of other amines. The range of application of this document concerning the concentrations of amines in indoor air depends on the linear range of the calibration line and hence on the gas sample volume (here: from 5 l up to 100 l), the eluate volume (from 1 ml up to 5 ml), the injection volume (from 1 µl up to 10 µl) and the sensitivity of the analytical equipment (e.g. linear range from 2 pg up to 2 ng amine). The range of application can be expected to be from approximately 0,002 µg/m3 (100 l sample) up to 2 000 µg/m3 (5 l sample) for a common analytical equipment (e.g. Waters ?TQD") for the majority of the amines listed in Tables A.1 and A.2. The analysis of derivatives of ethanolamine is usually about 10 times more sensitive and the analysis of short-chained aliphatic amines is usually about 10 times less sensitive than the analysis of an average amine. The performance data of the analytical method is given in Annex B, particularly in Tables B.1 and B.2. This document can be used also for the determination of amines in water if the detection limit is sufficient. This document does not cover the determination of isocyanates in indoor air (nor in water samples) as corresponding amines (covered by ISO 17734-1 and ISO 17734-2).
Air intérieur — Partie 39: Titre manque
Notranji zrak - 39. del: Določevanje aminov - Analiza aminov s tekočinsko kromatografijo visoke ločljivosti (HPLC), sklopljeno z masno spektrometrijo visoke ločljivosti (HRMS) ali tandemsko masno spektrometrijo (MS-MS)
Ta dokument, skupaj s standardom ISO 16000-38, določa metodo merjenja masne koncentracije primarnih, sekundarnih in terciarnih alifatskih ter aromatskih aminov v notranjem zraku z uporabo akumuliranega vzorčenja in visokozmogljive tekočinske kromatografije (HPLC) skupaj s tandemsko masno spektrometrijo (MS-MS) ali masno spektrometrijo visoke ločljivosti (HRMS). Dokument zajema analitični postopek. Postopek vzorčenja in izdelava vzorčevalnikov sta zajeta v standardu ISO 16000-38. Ta dokument opisuje specifikacije za kromatografijo in masno spektroskopijo aminov. Rezultati meritev so izraženi v μg/m3. Čeprav je dokument namenjen predvsem za merjenje aminov, naštetih v dodatku A.1 in A.2, se lahko uporablja tudi za merjenje drugih aminov v notranjem zraku. Dokument vsebuje navodila in opisuje postopke za vključitev drugih aminov. Področje uporabe tega dokumenta v zvezi s koncentracijami aminov v notranjem zraku je odvisno od linearnega obsega kalibracijske krivulje in s tem od volumna vzorca plina (tukaj: od 5 l do 100 l), volumna eluata (od 1 ml do 5 ml), volumna vbrizgavanja (od 1 μl do 10 μl) in občutljivosti analitične opreme (npr. linearni razpon od 2 pg do 2 ng amina). Področje uporabe je mogoče pričakovati od približno 0,002 μg/m3 (vzorec 100 l) do 2000 μg/m3 (vzorec 5 l) za skupno analitsko opremo (npr. vode »TQD«) za večino aminov iz preglednic A.1 in A.2. Analiza derivatov etanolamina je običajno približno 10-krat občutljivejša, analiza kratko verižnih alifatskih aminov pa je običajno približno 10-krat manj občutljiva kot
analiza povprečnega amina. Podatki o učinkovitosti analitske metode so navedeni v dodatku B, zlasti v preglednicah B.1 in B.2. Ta dokument je mogoče uporabiti tudi za določanje aminov v vodi, če je meja zaznavanja zadostna. Ta dokument ne zajema določevanja izocianatov kot ustreznih aminov v notranjem zraku (niti v vzorcih vode) (zajeto v standardih ISO 17734-1 in ISO 17734-2).
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST ISO 16000-39:2019
01-december-2019
Notranji zrak - 39. del: Določevanje aminov - Analiza aminov s tekočinsko
kromatografijo visoke ločljivosti (HPLC), sklopljeno z masno spektrometrijo visoke
ločljivosti (HRMS) ali tandemsko masno spektrometrijo (MS-MS)
Indoor air - Part 39: Determination of amines - Analysis of amines by (ultra-) high-
performance liquid chromatography coupled to high resolution or tandem mass
spectrometry
Ta slovenski standard je istoveten z: ISO 16000-39:2019
ICS:
13.040.20 Kakovost okoljskega zraka Ambient atmospheres
SIST ISO 16000-39:2019 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST ISO 16000-39:2019
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SIST ISO 16000-39:2019
INTERNATIONAL ISO
STANDARD 16000-39
First edition
2019-06
Indoor air —
Part 39:
Determination of amines —
Analysis of amines by (ultra-) high-
performance liquid chromatography
coupled to high resolution or tandem
mass spectrometry
Reference number
ISO 16000-39:2019(E)
©
ISO 2019
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SIST ISO 16000-39:2019
ISO 16000-39:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
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SIST ISO 16000-39:2019
ISO 16000-39:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Amines in indoor air . 2
4.1 Properties of amines . 2
4.2 Origin and occurrence of amines . 2
5 Analytical procedure . 2
5.1 Preparation of sample solutions . 2
5.2 High-performance liquid chromatography . 2
5.3 Tandem mass spectrometry . 3
5.4 High resolution mass spectrometry . 3
5.5 Sample sequence and external calibration . 3
6 Testing of the suitability of the equipment and the instrumental analysis .
...
INTERNATIONAL ISO
STANDARD 16000-39
First edition
2019-06
Indoor air —
Part 39:
Determination of amines —
Analysis of amines by (ultra-) high-
performance liquid chromatography
coupled to high resolution or tandem
mass spectrometry
Reference number
ISO 16000-39:2019(E)
©
ISO 2019
---------------------- Page: 1 ----------------------
ISO 16000-39:2019(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
---------------------- Page: 2 ----------------------
ISO 16000-39:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Amines in indoor air . 2
4.1 Properties of amines . 2
4.2 Origin and occurrence of amines . 2
5 Analytical procedure . 2
5.1 Preparation of sample solutions . 2
5.2 High-performance liquid chromatography . 2
5.3 Tandem mass spectrometry . 3
5.4 High resolution mass spectrometry . 3
5.5 Sample sequence and external calibration . 3
6 Testing of the suitability of the equipment and the instrumental analysis .3
6.1 General . 3
6.2 High-performance or ultra-performance liquid chromatography (HPLC or UHPLC) . 3
6.3 Tandem mass spectrometry (MS-MS) . 4
6.4 High resolution mass spectrometry (HRMS) . 4
7 Reporting requirements . 4
Annex A (informative) List of included amines .
...
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