ISO 17995:2019
(Main)Water quality — Detection and enumeration of thermotolerant Campylobacter spp
Water quality — Detection and enumeration of thermotolerant Campylobacter spp
This document specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection of Campylobacter species in a specified sample volume. Clean water samples with low turbidity can be membrane filtered for either a qualitative method, semi-quantitative or quantitative (MPN) method. Water samples with higher turbidity, such as primary and secondary wastewater effluents and sediments, are analysed using the same qualitative, semi-quantitative or quantitative MPN method by direct inoculation of material into bottles or tubes. Sediments can be suspended in a suitable diluent or inoculated directly into enrichment broths. Users wishing to employ this method are expected to verify its performance for the particular matrix under their own laboratory conditions.
Qualité de l'eau — Recherche et dénombrement d'espèces thermotolérantes du genre Campylobacter
Kakovost vode - Ugotavljanje prisotnosti in števila termotolerantnih vrst Campylobacter
Ta standard določa metodo za ugotavljanje prisotnosti ter izračuna delnega kvantitativnega in kvalitativnega (MPN) števila termotolerantnih bakterij iz roda Campylobacter. Metodo je mogoče uporabiti za vse vrste voda, vključno s: pitno vodo, podzemno vodo in vodo iz vodnjakov, sladko, slankasto in slano površinsko vodo, vodo v bazenih, vodo v zdraviliških bazenih in bazenih za hidroterapijo, rekreacijsko vodo, kmetijsko vodo in iztoki, neočiščeno in očiščeno odpadno vodo ter tudi vključno s peskom in drugimi usedlinami. Metodo je mogoče uporabiti za odkrivanje bakterij iz rodu Campylobacter v določeni količini vzorca. Vzorce čiste vode z nizko motnostjo je mogoče filtrirati skozi membrano bodisi s kvalitativno metodo, delno kvantitativno ali kvantitativno (MPN) metodo. Vzorci vode z večjo motnostjo, na primer primarne in sekundarne odpadne vode ter usedline, se analizirajo po isti kvalitativni, delni kvantitativni ali kvantitativni metodi MPN z neposredno inokulacijo materiala v steklenice ali epruvete. Usedline je mogoče odložiti v primerno razredčilo ali inokulirati neposredno v obogatitvene bujone. Od uporabnikov, ki želijo uporabiti to metodo, se pričakuje, da bodo v svojih laboratorijskih pogojih preverili učinkovitost metode za ustrezno matriko.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2020
Nadomešča:
SIST ISO 17995:2007
Kakovost vode - Ugotavljanje prisotnosti in števila termotolerantnih vrst
Campylobacter
Water quality - Detection and enumeration of thermotolerant Campylobacter species
Qualité de l'eau - Recherche et dénombrement d'espèces thermotolérantes du genre
Campylobacter
Ta slovenski standard je istoveten z: ISO 17995:2019
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
INTERNATIONAL ISO
STANDARD 17995
Second edition
2019-11
Water quality — Detection and
enumeration of thermotolerant
Campylobacter spp
Qualité de l'eau — Recherche et dénombrement d'espèces
thermotolérantes du genre Campylobacter
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Inoculation and enrichment in selective liquid medium . 2
4.3 Isolation and selection for confirmation . 2
4.4 Confirmation . 2
5 Culture media and reagents . 3
6 Apparatus . 3
7 Sampling, transport and storage . 4
8 Sample processing . 4
8.1 General . 4
8.2 Direct inoculation into enrichment broth . 4
8.3 Membrane filtration . 4
9 Procedure. 5
9.1 Enrichment . 5
9.2 Plating on solid, selective medium. 6
9.3 Reading of presumptive results . 6
9.4 Confirmation . 6
9.4.1 General. 6
9.4.2 Selection of colonies for confirmation . 6
9.4.3 Absence of growth on non-selective agar plates . 7
9.4.4 Motility and cell morphology . 7
9.4.5 Detection of oxidase activity . 7
9.4.6 Interpretation . 7
9.4.7 Further verification . 8
9.5 Identification of Campylobacter species (optional). 8
9.5.1 General. 8
9.5.2 Detection of catalase . . 8
9.5.3 Detection of hippurate hydrolysis . 8
9.5.4 Detection of indoxyl acetate hydrolysis . 9
9.5.5 Interpretation . 9
10 Quality assurance . 9
11 Expression of results . 9
12 Test report .10
Annex A (normative) Flow diagram of the method .11
Annex B (normative) Semi‑quantitative analysis .12
Annex C (normative) Composition and preparation of culture media and reagents .13
Annex D (normative) Performance testing for the quality assurance of the culture media .22
Annex E (informative) Performance characteristics .23
Annex F (informative) Additional information about campylobacters .24
Bibliography .25
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
This second edition cancels and replaces the first edition (ISO 17995:2005), which has been technically
revised. The main changes compared to the previous edition are as follows:
— the inclusion of direct inoculation of enrichment broths in addition to membrane filtration with
additional information about sample processing.
— methods for the speciation of Campylobacter.
— performance testing for the quality assurance of culture media has been added to Annex D.
— performance characteristics of the method have been added as an Annex E.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2019 – All rights reserved
Introduction
The thermotolerant Campylobacter species are not thought to propagate as free living but are zoonotic
pathogenic bacteria of mammals and birds and which cause disease in humans. Campylobacter
jejuni subsp. jejuni and Campylobacter coli are common causes of intestinal infections in humans.
Campylobacter upsaliensis is found mainly in cats and dogs and is of minor importance for human
infections. Campylobacter lari is less frequently associated with human infections. Campylobacter
infections give rise to a flu-like illness with malaise, fever and myalgia followed by diarrhoea. The
vehicles for Campylobacter infections are usually food, farm animals, pets and person-to-person
contact; water is also important. They can be isolated from waters contaminated with human or animal
faeces such as wastewater and surface waters. The bacteria have been demonstrated to survive within
amoebae. Outbreaks of campylobacteriosis have been reported in relation to the use of contaminated
drinking water and sporadic cases from recreational water use.
INTERNATIONAL STANDARD ISO 17995:2019(E)
Water quality — Detection and enumeration of
thermotolerant Campylobacter spp
WARNING — Persons using this docu
...
INTERNATIONAL ISO
STANDARD 17995
Second edition
2019-11
Water quality — Detection and
enumeration of thermotolerant
Campylobacter spp
Qualité de l'eau — Recherche et dénombrement d'espèces
thermotolérantes du genre Campylobacter
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 General . 2
4.2 Inoculation and enrichment in selective liquid medium . 2
4.3 Isolation and selection for confirmation . 2
4.4 Confirmation . 2
5 Culture media and reagents . 3
6 Apparatus . 3
7 Sampling, transport and storage . 4
8 Sample processing . 4
8.1 General . 4
8.2 Direct inoculation into enrichment broth . 4
8.3 Membrane filtration . 4
9 Procedure. 5
9.1 Enrichment . 5
9.2 Plating on solid, selective medium. 6
9.3 Reading of presumptive results . 6
9.4 Confirmation . 6
9.4.1 General. 6
9.4.2 Selection of colonies for confirmation . 6
9.4.3 Absence of growth on non-selective agar plates . 7
9.4.4 Motility and cell morphology . 7
9.4.5 Detection of oxidase activity . 7
9.4.6 Interpretation . 7
9.4.7 Further verification . 8
9.5 Identification of Campylobacter species (optional). 8
9.5.1 General. 8
9.5.2 Detection of catalase . . 8
9.5.3 Detection of hippurate hydrolysis . 8
9.5.4 Detection of indoxyl acetate hydrolysis . 9
9.5.5 Interpretation . 9
10 Quality assurance . 9
11 Expression of results . 9
12 Test report .10
Annex A (normative) Flow diagram of the method .11
Annex B (normative) Semi‑quantitative analysis .12
Annex C (normative) Composition and preparation of culture media and reagents .13
Annex D (normative) Performance testing for the quality assurance of the culture media .22
Annex E (informative) Performance characteristics .23
Annex F (informative) Additional information about campylobacters .24
Bibliography .25
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
This second edition cancels and replaces the first edition (ISO 17995:2005), which has been technically
revised. The main changes compared to the previous edition are as follows:
— the inclusion of direct inoculation of enrichment broths in addition to membrane filtration with
additional information about sample processing.
— methods for the speciation of Campylobacter.
— performance testing for the quality assurance of culture media has been added to Annex D.
— performance characteristics of the method have been added as an Annex E.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2019 – All rights reserved
Introduction
The thermotolerant Campylobacter species are not thought to propagate as free living but are zoonotic
pathogenic bacteria of mammals and birds and which cause disease in humans. Campylobacter
jejuni subsp. jejuni and Campylobacter coli are common causes of intestinal infections in humans.
Campylobacter upsaliensis is found mainly in cats and dogs and is of minor importance for human
infections. Campylobacter lari is less frequently associated with human infections. Campylobacter
infections give rise to a flu-like illness with malaise, fever and myalgia followed by diarrhoea. The
vehicles for Campylobacter infections are usually food, farm animals, pets and person-to-person
contact; water is also important. They can be isolated from waters contaminated with human or animal
faeces such as wastewater and surface waters. The bacteria have been demonstrated to survive within
amoebae. Outbreaks of campylobacteriosis have been reported in relation to the use of contaminated
drinking water and sporadic cases from recreational water use.
INTERNATIONAL STANDARD ISO 17995:2019(E)
Water quality — Detection and enumeration of
thermotolerant Campylobacter spp
WARNING — Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety problems, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this document
be carried out by suitably trained staff.
1 Scope
This document specifies a method for the detection, semi-quantitative and quantitative (MPN)
enumeration of thermotolerant Campylobacter species.
The method can be applied to all kinds of waters including: drinking water, ground water and well
water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools,
recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand
and other sediments.
This method can be used for the detection of Campylobacter species in a specified sample volume. Clean
water samples with low turbidity can be membrane filtere
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.