SIST EN 370:2024
(Main)Wood preservatives - Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer)
Wood preservatives - Determination of eradicant efficacy in preventing emergence of Anobium punctatum (De Geer)
This document specifies a method for the determination of the curative action of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood.
This method is applicable to any surface-applied treatment that is intended to prevent emergence of adult beetles but not intended to kill larvae in infested timber.
NOTE 1 This method can be used in conjunction with an ageing procedure, for example EN 73.
NOTE 2 Products intended to kill larvae can be tested by the method described in EN 48.
Holzschutzmittel - Bestimmung der auf Schlupfverhinderung beruhenden bekämpfenden Wirksamkeit gegenüber Anobium punctatum (De Geer)
Dieses Dokument legt ein Verfahren zur Bestimmung der bekämpfenden Wirkung eines Holzschutzmittels gegen Befall durch Anobium punctatum (De Geer) fest, wenn das Produkt zur Oberflächenbehandlung auf Holz aufgetragen wird.
Dieses Verfahren ist für jede Oberflächenbehandlung anwendbar, die dazu bestimmt ist, den Schlupf ausgewachsener Käfer zu verhindern, aber nicht dazu gedacht ist, die Larven in befallenem Holz abzutöten.
ANMERKUNG 1 Dieses Verfahren kann in Verbindung mit einer Alterungsbeanspruchung angewendet werden, z. B. nach EN 73.
ANMERKUNG 2 Produkte zur Abtötung von Larven können durch das in EN 48 beschriebene Verfahren geprüft werden.
Produits de préservation du bois - Détermination de l'efficacité curative contre l'émergence d'Anobium punctatum (De Geer)
Le présent document décrit une méthode de détermination de l’action curative d'un produit de préservation du bois contre l’infestation d'Anobium punctatum (De Geer) lorsque le produit est appliqué au bois par un traitement de surface.
La présente méthode est applicable à tout traitement de surface qui est destiné à empêcher l'émergence d’insectes adultes mais non de tuer les larves dans le bois infesté.
NOTE 1 Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple selon l'EN 73.
NOTE 2 Les produits destinés à tuer les larves peuvent être soumis à essai selon la méthode décrite dans l’EN 48.
Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivnega delovanja proti navadnemu trdoglavcu Anobium punctatum (De Geer)
Ta dokument določa metodo za ugotavljanje učinkovitosti obnovitvenega delovanja zaščitnega sredstva za les proti navadnemu trdoglavcu Anobium punctatum (De Geer), ko je izdelek uporabljen v sklopu površinske obdelave
lesa.
Ta metoda se uporablja za vse površinske obdelave lesa, ki so namenjene preprečevanju pojavu odraslih hroščev, ne pa za ubijanje ličink v prizadetem lesu.
OPOMBA 1: Ta metoda se lahko uporablja v povezavi s postopkom staranja, na primer EN 73.
OPOMBA 2: Proizvode, namenjene za ubijanje ličink, je mogoče preskusiti po metodi, opisani v standardu EN 48.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2024
Nadomešča:
SIST EN 370:2004
Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivnega delovanja proti
navadnemu trdoglavcu Anobium punctatum (De Geer)
Wood preservatives - Determination of eradicant efficacy in preventing emergence of
Anobium punctatum (De Geer)
Holzschutzmittel - Bestimmung der auf Schlupfverhinderung beruhenden bekämpfenden
Wirksamkeit gegenüber Anobium punctatum (De Geer)
Produits de préservation du bois - Détermination de l'efficacité curative contre
l'émergence d'Anobium punctatum (De Geer)
Ta slovenski standard je istoveten z: EN 370:2023
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 370
EUROPEAN STANDARD
NORME EUROPÉENNE
December 2023
EUROPÄISCHE NORM
ICS 71.100.50 Supersedes EN 370:1993
English Version
Wood preservatives - Determination of eradicant efficacy
in preventing emergence of Anobium punctatum (De Geer)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der auf
l'efficacité curative contre l'émergence d'Anobium Schlupfverhinderung beruhenden bekämpfenden
punctatum (De Geer) Wirksamkeit gegenüber Anobium punctatum (De Geer)
This European Standard was approved by CEN on 6 November 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 370:2023 E
worldwide for CEN national Members.
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 6
5 Test materials and apparatus . 7
5.1 Biological material . 7
5.1.1 Anobium punctatum (De Geer) larvae . 7
5.1.2 Provision of larvae . 7
5.1.3 Choice of larvae . 7
5.2 Products and reagents . 7
5.3 Apparatus . 8
6 Sampling . 8
7 Test specimens . 8
7.1 Species of wood . 8
7.2 Quality of wood . 8
7.3 Provision of test specimens . 9
7.4 Dimension of test specimens . 9
7.5 Number of test specimens . 9
8 Procedure. 9
8.1 Preparation of the test specimens . 9
8.1.1 Sealing of the transverse faces . 9
8.1.2 Treatment of test specimens . 10
8.2 Exposure of the test specimens to the insects . 11
8.2.1 Preparation of sub-specimens . 11
8.2.2 Insertion of larvae. 11
8.2.3 Conditioning of infested sub-specimens to induce emergence . 11
8.3 Examination of sub-specimens . 12
9 Validity of the test . 12
10 Expression of results . 12
11 Test report . 12
Annex A (informative) Example of a test report . 16
Annex B (informative) Culturing technique for Anobium punctatum . 18
B.1 Culture wood . 18
B.1.1 Wood species . 18
B.1.2 Collection of culture wood . 18
B.1.3 Cutting of culture wood . 18
B.1.4 Drying of culture wood . 18
B.2 Source of beetles . 18
B.2.1 Collection of beetles . 18
B.2.2 Quarantine of beetles . 18
B.3 Infestation of culture wood . 18
B.3.1 Culture vessels . 18
B.3.2 Preparation of wood . 19
B.3.3 Introduction of beetles . 19
B.4 Culturing conditions . 19
B.4.1 Normal environment . 19
B.4.2 Natural pupation induction . 19
B.4.3 Artificial pupation induction . 19
B.5 Collection of beetles . 19
B.6 General culture hygiene . 19
Bibliography . 21
European foreword
This document (EN 370:2023) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by SIS.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by June 2024, and conflicting national standards shall be
withdrawn at the latest by June 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 370:1993.
The main changes compared to the previous edition are listed below:
a) maximum recommended quantity of test solution application was changed from 250 g/m to
300 ml/m (8.1.2.2);
b) the count of adults for validity of the test may include those hatching underneath the cover-ups of
the end faces (Clause 9).
NOTE Test results obtained according to earlier versions of this document and when the tests had started
before this version of EN 370 was published are considered as valid.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
This document describes a laboratory method of test which gives a basis for assessment of the eradicant
efficacy of a wood preservative, in preventing emergence of Anobium punctatum. It determines the lethal
effects of an insecticidal product, deposited by surface application, on beetles attempting to emerge
through treated wood surfaces.
The method has been developed to assess the efficacy of eradicant formulations based on non-
penetrating fluids which act only on emerging adult beetles and not at depth on larvae established in the
wood.
The method simulates conditions which can appear in practice where a length of timber infested with
Anobium punctatum is treated on all the sides from which emergence of beetles is possible.
This laboratory method provides one criterion by which the value of a product can be assessed. In making
this assessment, the methods by which the preservative may be applied should be taken into account. It
is further recommended that results from this test should be supplemented by those from other
appropriate tests, and above all by comparison with practical experience.
When products which are very active at low concentrations are used, it is very important to take suitable
precautions to isolate and separate, as far as possible, operations involving chemical products, other
products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use
of separate rooms, areas within rooms, extraction facilities and conditioning chambers as well as special
training for personnel.
1 Scope
This document specifies a method for the determination of the curative action of a wood preservative
against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment
to wood.
This method is applicable to any surface-applied treatment that is intended to prevent emergence of adult
beetles but not intended to kill larvae in infested timber.
NOTE 1 This method can be used in conjunction with an ageing procedure, for example EN 73.
NOTE 2 Products intended to kill larvae can be tested by the method described in EN 48.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
EN ISO 835, Laboratory glassware — Graduated pipettes (ISO 835)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics
of the total volume being sampled
3.2
supplier
sponsor of the test
4 Principle
Preservative is applied by brush or pipette onto test specimens of a susceptible timber. After drying the
test specimens are cut into two sub-specimens and larvae of Anobium punctatum are introduced into the
freshly-cut end grain surfaces.
After allowing larvae to establish, the untreated faces are sealed and insects are induced to pupate and
emerge. The numbers of beetles that emerge and the population that remains within the specimens are
compared with those in untreated controls.
5 Test materials and apparatus
5.1 Biological material
5.1.1 Anobium punctatum (De Geer) larvae
The culturing technique, which experience has shown to be suitable, is described in Annex B.
5.1.2 Provision of larvae
Carefully split or crumble infested small branchwood to extract larvae. Examine them under a binocular
microscope and destroy any that show injury or mite infestation or that do not respond by movement
when touched.
Weigh the larvae and keep those that have a mass between 7 mg and 12 mg, and are in perfect condition.
Keep them, for between 12 h and 60 h, separately from one another in glass receptacles in the culturing
chamber (5.3.1). Re-examine them and reject any which do not show movement in response to
stimulation with a fine brush.
5.1.3 Choice of larvae
Select sets of 12 larvae so that the total mass of each set is between 100 mg and 125 mg.
The numbers of larvae required are shown in Table 1.
Table 1 — Numbers of larvae
Number of test specimens
(100 mm × 50 mm × 30 mm)
Number of
Total number of
formulations to be required
larvae required
tested
Untreated Treated
controls specimens
1 3 3 144
2 3 6 216
3 6 9 360
4 6 12 432
NOTE 1 Each test specimen will later be divided into two sub-specimens (see 8.2).
NOTE 2 Additional larvae can be required to replace larvae which do not establish in the test sub-specimens.
5.2 Products and reagents
5.2.1 Water, complying with grade 3 of ISO 3696.
5.2.2 Gelatin, for sealing the relevant surfaces of specimens to be treated with solutions in which an
organic solvent is the continuous phase.
5.2.3 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which
water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity
(75 ± 5) %.
5.3.2 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid
excessive exposure of operators to solvents or their vapours.
(75 ± 5) %.
5.3.3 Testing chamber, ventilated, controlled at (21 ± 2) °C and at relative humidity
5.3.4 Low temperature regime chamber
— Either:
— ventilated and controlled to provide a continuous temperature regime with consecutive cycles
of 12 h at (6 ± 1) °C and 12 h at (13 ± 1) °C; or
— ventilated and controlled at (6 ± 1) °C and relative humidity (70 ± 5) %.
5.3.5 Drill, provided with bits capable of drilling smooth cylindrical holes of 2 mm diameter in
wood.
5.3.6 Plastic plates of opaque unplasticised PVC, 50 mm × 30 mm × 1 mm.
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent,
to ensure the safety of the operator.
5.3.8 Pipette, of type specified in EN ISO 835, Class B: graduated pipette with no waiting time. Capacity
from 0,5 ml to 25 ml with a maximum permissible error of ± 0,01 ml.
5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of
0,01 g.
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored
and handled in accordance with any written recommendations from the supplier.
For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The test shall be carried out on Pinus sylvestris (Linnaeus), Scots pine.
Additional tests may be done with other species such as European beech (Fagus sylvatica) (Linnaeus) but,
if so, this should be stated in the test report.
7.2 Quality of wood
Use only sound sapwood, straight-grained and without knots and bark.
The wood shall have an average growth of between two an
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