SIST EN 49-1:2005
(Main)Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)
Wood preservatives - Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface treatment (Laboratory method)
This document specifies a method for the determination of the protective effectiveness or the toxic values of a wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface treatment to wood.
This method is applicable to:
3 water-insoluble chemicals which are being studied as active insecticides,
3 organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,
3 organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates,
3 water-soluble materials, for example salts.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven - Teil 1: Oberflächenbehandlung (Laboratoriumsverfahren)
Dieses Dokument legt ein Verfahren zur Bestimmung der Schutzwirkung oder der Grenze der Wirksamkeit eines Holzschutzmittels gegen den Befall durch Anobium punctatum (De Geer) fest, wenn das Produkt zur Oberflächenbehandlung des Holzes verwendet wird.
Das Verfahren gilt für:
- wasserunlösliche Chemikalien, die als wirksame Insektizide untersucht werden;
- organische Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten;
- organische, in Wasser dispergierbare Formulierungen in deren Anlieferungszustand oder in Form von im Laboratorium hergestellten Verdünnungen von Konzentraten; und
- wasserlösliche Stoffe, zum Beispiel Salze.
ANMERKUNG Das Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung, zum Beispiel nach EN 73, angewendet werden.
Produits de préservation du bois - Détermination de l'efficacité protectrice vis a vis d' Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)
La présente partie de l'EN 49 prescrit une méthode de détermination de l'efficacité protectrice ou du seuil d'efficacité d'un produit de préservation du bois contre l'infestation par Anobiurn punctatum (De Geer) lorsque le produit est appliqué au bois par un traitement de surface.
La présente méthode est applicable :
3 aux produits chimiques non hydrosolubles étudiés en tant que matieres actives insecticides ; ou
3 aux formules organiques telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ; ou
3 aux formulations organiques hydrodispersables telles qu'elles sont fournies ou préparées au laboratoire par dilution de concentrés ; ou
3 aux produits solubles dans l'eau, par exemple aux sels.
NOTE Cette méthode peut etre utilisée conjointement avec une épreuve de vieillisse ment, par exemple EN 73.
Zaščitna sredstva za les - Ugotavljanje učinkovitosti preventivne zaščite proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število položenih jajčec in preživelih ličink - 1. del: Površinsko nanašanje (Laboratorijska metoda)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN 49-1:2005
01-julij-2005
1DGRPHãþD
SIST EN 49-1:1996
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHXþLQNRYLWRVWLSUHYHQWLYQH]DãþLWHSURWL
QDYDGQHPXWUGRJODYFX$QRELXPSXQFWDWXP 'H*HHU JOHGHQDãWHYLORSRORåHQLK
MDMþHFLQSUHåLYHOLKOLþLQNGHO 3RYUãLQVNRQDQDãDQMH /DERUDWRULMVNDPHWRGD
Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 1: Oberflächenbehandlung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis a vis d'
Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-1:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST EN 49-1:2005 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN 49-1:2005
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SIST EN 49-1:2005
EUROPEAN STANDARD
EN 49-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 49-1:1992
English version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'efficacité protectrice vis à vis d' Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch
Geer) par l'observation de la ponte et du taux de survie des Beobachten der Eiablage und des Überlebens von Larven -
larves - Partie 1: Application par traitement de surface Teil 1: Oberflächenbehandlung (Laborverfahren)
(Méthode de laboratoire)
This European Standard was approved by CEN on 3 February 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-1:2005: E
worldwide for CEN national Members.
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SIST EN 49-1:2005
EN 49-1:2005 (E)
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative references .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.7
7 Test specimens.7
8 Procedure .8
9 Validity of test .11
10 Expression of results .11
11 Test report .12
Annex A (informative) Example of a test report .14
Annex B (informative) Identification of sex of test insects Anobium punctatum.16
Annex C (informative) Culturing technique for Anobium punctatum .17
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory .20
Bibliography .21
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SIST EN 49-1:2005
EN 49-1:2005 (E)
Foreword
This document (EN 49-1:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and
wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical text or
by endorsement, at the latest by September 2005, and conflicting national standards shall be withdrawn at the
latest by September 2005.
This document supersedes EN 49-1:1992.
This document consists of two parts, Part 1 is required to enable effectiveness assessments of wood preservatives
which are intended to be applied by surface treatment and Part 2 those which are intended to be applied by
impregnation.
Significant technical differences between this document and EN 49-1:1992 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental and
health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark,
Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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SIST EN 49-1:2005
EN 49-1:2005 (E)
Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of
a wood preservative, when applied as a surface treatment, against Anobium punctatum. It allows the determination
of the concentration at which the product prevents the development of infestation from egg laying. It can also be
used with formulations ready for use.
The method simulates conditions which can occur in practice on timber which has been treated some time
previously with wood preservative applied by dip, brush or spray and on which eggs of Anobium punctatum are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative may be applied should be taken into account. It is further
recommended that results from this test should be supplemented by those from other appropriate tests, and above
all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable precautions
to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood,
laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within
rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for
environmental, health and safety precautions).
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SIST EN 49-1:2005
EN 49-1:2005 (E)
1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic values of a
wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface
treatment to wood.
This method is applicable to:
water-insoluble chemicals which are being studied as active insecticides,
organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,
organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates,
and
water-soluble materials, for example salts.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references,
only the edition cited applies. For undated references the latest edition of the referenced document (including any
amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
ISO 835-1, Laboratory glassware – Graduated pipettes – Part 1: General requirements
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total
volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Depending on the test being carried out either:
on a set of test specimens of a susceptible wood species that is surface treated with a solution of the
preservative, or
if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are
surface treated with a series of solutions in which the concentration of preservative is ranged in a given
progression.
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SIST EN 49-1:2005
EN 49-1:2005 (E)
The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid, the
number of eggs hatched and the numbers of surviving larvae are observed and compared with those in untreated
control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by
dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test
specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture
(see Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then
checked to ensure that they are undamaged, active and free from any infestation by mites. Determine the sex
(see Annex B) of the collected and checked adults and place the males and females in separate containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is
the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase.
5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and
insoluble in the product under test.
NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable.
5.2.4 Water, complying with grade 3 of EN ISO 3696.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue
in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth, of cotton or linen, with a mesh aperture of 0,3 mm to 0,6 mm.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
NOTE The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has
the conditions specified for the conditioning chamber (see 5.3.2).
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5.3.3 Treatment vessel, of a material that does not react with the preservative under test, for example of glass
for organic products and of polyethylene for salts containing fluorine.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid
excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.6 Pipette, of type specified in ISO 835-1, Class B: graduated pipette with no waiting time. Capacity 1 ml with
an accuracy of ± 0,01 ml.
5.3.7 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to
ensure the safety of the operator.
5.3.8 Test containers, suitable for holding the test specimens and of material resistant to the solvents used, and
fitted with perforated covers to provide a good exchange of air.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.10 X-ray apparatus, (optional) with tungsten target and beryllium window, with voltage and current
continuously variable in the ranges:
voltage: 10 kV to 50 kV;
current: 0 mA to 15 mA.
5.3.11 Protective gloves
6 Sampling
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and
handled in accordance with any written recommendations from the supplier.
NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The reference species is European oak. This shall be either sessile oak (Quercus petraea (Mattuschka) Lieblin) or
pedunculate oak (Quercus robur Linnaeus).
1)
NOTE Additional tests may be carried out using other species but, if so, this should be stated in the test report.
7.2 Wood quality
The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have
been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in
1) The growth of young larvae of Anobium punctatum is slow in specimens from resinous wood. Results from test specimens
in resinous wood should be compared with those obtained from oak specimens.
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EN 49-1:2005 (E)
winter. The trees shall be cut immediately after felling and the timber rapidly air-dried. The wood shall not have
been stored for more than three years.
2)
The wood shall be exclusively sapwood and having between 2 annual rings per 10 mm and 10 annual rings per
10 mm.
NOTE It is recommended to use test specimens of similar growth rate within a single test.
7.3 Provision of test specimens
3)
Prepare planed strips having a cross-section of (25 ± 0,5) mm x (15 ± 0,5) mm removing a minimum of 2 mm
from any surfaces exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The
annual rings shall be parallel to the broad faces (contact angle of less than 5°). Make transverse cuts, neatly to give
sharp edges and a fine-sawn finish to the end-grain surfaces, to give test specimens (50 ± 0,5) mm long.
The specimens shall originate from a minimum of three trees or shall be taken at random from a stock originally of
more than 500 test specimens.
7.4 Dimensions of test specimens
The dimensions of each test specimen after reaching equilibrium in the conditioning chamber (5.3.2) shall be
(50 ± 0,5) mm x (25 ± 0,5) mm x (15 ± 0,5) mm.
Mark each test specimen so that it can be identified throughout the test.
7.5 Number of test specimens
Use:
a) five test specimens (see 7.4) for each preservative and each concentration;
b) five untreated control test specimens (see 7.4) for a complete test of any given preservative;
c) five control test specimens (7.4) treated with that solvent or diluent (5.2.4 or 5.2.5). if a solvent or diluent (water
included) is used.
When dipping is to be used (8.1.3.2.1) it is advisable to treat more than the specified number of test specimens so
that, after weighing, any test specimens with abnormally high or low retentions can be rejected from the batch.
8 Procedure
8.1 Preparation of the test specimens
8.1.1 Conditioning of test specimens prior to sealing
Allow the test specimens to condition in the conditioning chamber (5.3.2), for a minimum of two weeks.
8.1.2 Sealing
Sealing of the narrower longitudinal and the transverse faces and one of the large faces of the test specimens.
Seal these faces as follows:
2) It is not essential in this test for the starch content of the wood to be high.
3) These test specimens may be taken from the trunk of the tree or the large branches.
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8.1.2.1 For tests with solutions in which water is the continuous phase, apply three coats of the paraffin
wax (5.2.1) at about 90 °C so that the first coat adheres closely to the wood and the successive coatings bond to
one another. Condition the sealed test specimens in the conditioning chamber (5.3.2) for at least one day.
8.1.2.2 For tests with preservative solutions in which the continuous phase is an organic solvent that
dissolves paraffin wax, use the gelatine (5.2.2): apply the first coat as an aqueous solution of 200 g/l at 40 °C, then
after a minimum of 8 h of drying, apply two further coats of an aqueous solution of 300 g/l at 50 °C. Condition the
sealed test specimens in the conditioning chamber (5.3.2) for at least one day.
8.1.3 Treatment of the test specimens
8.1.3.1 Preparation of treatment solutions
8.1.3.1.1 Solid preservatives
Water-soluble preservatives: dissolve the preservative in the water (5.2.4) to the required concentration, or in a
series of concentrations if toxic values are to be determined;
Non-water-soluble preservatives: dissolve the preservative in an appropriate solvent (5.2.5) to the required
concentration, or in a series of concentrations if toxic values are to be determined.
All treatment solutions shall be freshly prepared.
8.1.3.1.2 Liquid preservatives
If appropriate, use the preservative without further preparation other than any necessary stirring. If it is a
concentrate or if toxic values are to be determined, dilute the p
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