SIST EN ISO 11702:2016

Animal and vegetable fats and oils - Enzymatic determination of total sterols content (ISO 11702:2016)

This International Standard specifies a method for the quantitative determination of the total sterols
content by means of an enzymatic staining test. The method is applicable to free and esterified sterols
in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable
to sample quantities of 1 g to 2 g of fat.
The method is not applicable to dark coloured fats and oils. The enzyme is not specific for cholesterol,
but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with
sterols at higher levels.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this
International Standard.
NOTE The method is technically equivalent to IUPAC method 2.404[8] and DGF standard method F-III 2 (91).[7]

Tierische und pflanzliche Fette und Öle - Enzymatische Bestimmung des Gesamtsterin-Gehaltes (ISO 11702:2016)

Diese Internationale Norm legt ein Verfahren zur quantitativen Bestimmung des Gesamtsterin-Gehaltes durch einen enzymatischen Farbtest fest. Das Verfahren ist anwendbar auf freie und veresterte Sterine in tierischen und pflanzlichen Fetten und Ölen, fetthaltigen Lebensmitteln und verwandten Erzeugnissen. Die Bestimmung gilt für Probenmengen von 1 g bis 2 g Fett.
Das Verfahren ist nicht auf dunkel gefärbte Fette und Öle anwendbar. Das Enzym ist nicht spezifisch für Cholesterin, sondern es werden auch andere 3 Hydroxysterine oxidiert. Das Verfahren wurde nicht an Produkten geprüft, die mit höheren Konzentrationen von Sterinen angereichert waren.
Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieser Internationalen Norm ausgenommen.
ANMERKUNG   Das Verfahren ist der IUPAC Methode 2.404 [8] und der DGF Einheitsmethode F III 2 (91) [7] technisch gleichwertig.

Corps gras d'origines animale et végétale - Détermination enzymatique de la teneur en stérols totaux (ISO 11702:2016)

L'ISO 11702 :2016 spécifie une méthode pour la  détermination quantitative de la teneur en stérols totaux à l'aide d'un essai de coloration enzymatique. La méthode s'applique aux stérols libres et estérifiés, contenus dans les corps gras d'origines animale et végétale, dans les aliments gras et les produits connexes. La détermination s'applique aux quantités d'échantillon de 1 g à 2 g de matière grasse.
Cette méthode ne s'applique pas aux corps gras de couleur sombre. L'enzyme n'est pas spécifique du cholestérol et oxyde également d'autres 3-hydroxystérols. La méthode n'a pas été testée pour des produits enrichis avec des stérols à des niveaux plus élevés.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application de l'ISO 11702 :2016.

Rastlinske in živalske maščobe in olja - Encimsko določevanje celotnih sterolov (ISO 11702:2016)

Ta mednarodni standard določa metodo za količinsko določevanje celotnih sterolov na podlagi encimskega preskusa z obarvanjem. Metoda se uporablja za proste in esterificirane sterole v rastlinskih in živalskih maščobah in oljih, živilih, ki vsebujejo maščobe, ter povezanih proizvodih. Določevanje se uporablja za vzorčne količine maščobe (1–2 g).
Metoda se ne uporablja za maščobe in olja temne barve. Encim ni specifičen za holesterol, vendar oksidira tudi druge 3-hidroksisterole. Metoda ni bila preskušena za proizvode, obogatene s steroli na višjih ravneh.
Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem mednarodnem standardu.
OPOMBA: Metoda je tehnično enakovredna metodi IUPAC 2.404[8] in standardni metodi DGF F-III 2 (91).

General Information

• Status: Published
• Public Enquiry End Date: 19-May-2016
• Publication Date: 06-Sep-2016
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 18-Aug-2016
• Due Date: 23-Oct-2016
• Completion Date: 07-Sep-2016
• Ref Project: EN ISO 11702:2016 - Animal and vegetable fats and oils - Enzymatic determination of total sterols content (ISO 11702:2016)

Relations

• Replaces: SIST EN ISO 11702:2010 - Animal and vegetable fats and oils - Enzymatic determination of total sterols content (ISO 11702:2009)
• Effective Date: 01-Oct-2016

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 11702:2016
01-oktober-2016
1DGRPHãþD
SIST EN ISO 11702:2010
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD(QFLPVNRGRORþHYDQMHFHORWQLKVWHURORY
,62
Animal and vegetable fats and oils - Enzymatic determination of total sterols content
(ISO 11702:2016)
Tierische und pflanzliche Fette und Öle - Enzymatische Bestimmung des Gesamtsterin-
Gehaltes (ISO 11702:2016)
Corps gras d'origines animale et végétale - Détermination enzymatique de la teneur en
stérols totaux (ISO 11702:2016)
Ta slovenski standard je istoveten z: EN ISO 11702:2016
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 11702:2016 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 11702:2016

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SIST EN ISO 11702:2016


EN ISO 11702
EUROPEAN STANDARD

NORME EUROPÉENNE

August 2016
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 11702:2009
English Version

Animal and vegetable fats and oils - Enzymatic
determination of total sterols content (ISO 11702:2016)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Enzymatische
Détermination enzymatique de la teneur en stérols Bestimmung des Gesamtsterin-Gehaltes (ISO
totaux (ISO 11702:2016) 11702:2016)
This European Standard was approved by CEN on 9 August 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11702:2016 E
worldwide for CEN national Members.

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SIST EN ISO 11702:2016
EN ISO 11702:2016 (E)
Contents Page
European foreword . 3
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SIST EN ISO 11702:2016
EN ISO 11702:2016 (E)
European foreword
This document (EN ISO 11702:2016) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2017, and conflicting national standards
shall be withdrawn at the latest by February 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 11702:2009.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 11702:2016 has been approved by CEN as EN ISO 11702:2016 without any modification.
3

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SIST EN ISO 11702:2016

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SIST EN ISO 11702:2016
INTERNATIONAL
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