Animal and vegetable fats and oils - Determination of acid value and acidity (ISO 660:2009)

This International Standard specifies three methods (two titrimetric and one potentiometric) for the determination of the acidity in animal and vegetable fats and oils, hereinafter referred to as fats. The acidity is expressed preferably as acid value, or alternatively as acidity calculated conventionally. This International Standard is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. The methods are not applicable to waxes. Since the methods are completely non-specific, they cannot be used to differentiate between mineral acids, free fatty acids, and other organic acids. The acid value, therefore, also includes any mineral acids that may be present.

Tierische und pflanzliche Fette und Öle - Bestimmung der Säurezahl und der Azidität (ISO 660:2009)

Diese Internationale Norm legt drei Verfahren (zwei titrimetrische und ein potentiometrisches) zur Bestimmung der Azidität in tierischen und pflanzlichen Fetten und Ölen, die im Folgenden als Fette bezeichnet werden, fest. Die Azidität wird vorzugsweise als Säurezahl ausgedrückt, alternativ als konventionell berechnete Azidität.
Die Norm gilt für raffinierte und rohe pflanzliche oder tierische Fette und Öle, Soapstock Fettsäuren oder technische Fettsäuren. Die Verfahren gelten nicht für Wachse.
Da die Verfahren vollkommen unspezifisch sind, können diese zur Unterscheidung zwischen Mineralsäuren, freien Fettsäuren und sonstigen organischen Säuren nicht angewendet werden. Die Säurezahl umfasst daher auch möglicherweise vorhandene Mineralsäuren.

Corps gras d'origines animale et végétale - Détermination de l'indice d'acide et de l'acidité (ISO 660:2009)

L'ISO 660:2009 spécifie trois méthodes (deux titrimétriques et une potentiométrique) pour la détermination de l'acidité dans les corps gras d'origines animale et végétale dits corps gras. L'acidité est exprimée, de préférence, en indice d'acide ou bien en acidité calculée conventionnellement.
L'ISO 660:2009 est applicable aux corps gras d'origines végétale ou animale à l'état brut ou raffinés, aux acides gras des pâtes de neutralisation ou aux acides gras de qualité technique. Ces méthodes ne sont pas applicables aux cires.
Ces méthodes ne sont en aucun cas spécifiques, elles ne peuvent donc pas être utilisées pour différencier les acides minéraux, les acides gras libres et autres acides organiques. De ce fait, l'indice d'acide inclut tout acide minéral potentiellement présent.

Rastlinske in živalske maščobe in olja - Določevanje kislinskega števila in kislosti (ISO 660:2009)

General Information

Status
Withdrawn
Publication Date
17-Nov-2009
Withdrawal Date
11-Oct-2020
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
12-Oct-2020
Due Date
04-Nov-2020
Completion Date
12-Oct-2020

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SLOVENSKI STANDARD
SIST EN ISO 660:2009
01-december-2009
1DGRPHãþD
SIST EN ISO 660:2000
SIST EN ISO 660:2000/A1:2005

5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHNLVOLQVNHJDãWHYLODLQNLVORVWL

,62

Animal and vegetable fats and oils - Determination of acid value and acidity (ISO

660:2009)

Tierische und pflanzliche Fette und Öle - Bestimmung der Säurezahl und der Azidität

(ISO 660:2009)

Corps gras d'origines animale et végétale - Détermination de l'indice d'acide et de

l'acidité (ISO 660:2009)
Ta slovenski standard je istoveten z: EN ISO 660:2009
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 660:2009 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 660:2009
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SIST EN ISO 660:2009
EUROPEAN STANDARD
EN ISO 660
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2009
ICS 67.200.10 Supersedes EN ISO 660:1999
English Version
Animal and vegetable fats and oils - Determination of acid value
and acidity (ISO 660:2009)

Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung der

de l'indice d'acide et de l'acidité (ISO 660:2009) Säurezahl und der Azidität (ISO 660:2009)

This European Standard was approved by CEN on 20 May 2009.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European

Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national

standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation

under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the

official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,

France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,

Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 660:2009: E

worldwide for CEN national Members.
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SIST EN ISO 660:2009
EN ISO 660:2009 (E)
Contents Page

Foreword ..............................................................................................................................................................3

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SIST EN ISO 660:2009
EN ISO 660:2009 (E)
Foreword

This document (EN ISO 660:2009) has been prepared by Technical Committee ISO/TC 34 "Food products" in

collaboration with Technical Committee CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their

by-products - Methods of sampling and analysis", the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an identical

text or by endorsement, at the latest by December 2009, and conflicting national standards shall be withdrawn

at the latest by December 2009.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN ISO 660:1999.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following

countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech

Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,

Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,

Sweden, Switzerland and the United Kingdom.
Endorsement notice

The text of ISO 660:2009 has been approved by CEN as a EN ISO 660:2009 without any modification.

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SIST EN ISO 660:2009
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SIST EN ISO 660:2009
INTERNATIONAL ISO
STANDARD 660
Third edition
2009-06-15
Animal and vegetable fats and oils —
Determination of acid value and acidity
Corps gras d'origines animale et végétale — Détermination de l'indice
d'acide et de l'acidité
Reference number
ISO 660:2009(E)
ISO 2009
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SIST EN ISO 660:2009
ISO 660:2009(E)
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ii © ISO 2009 – All rights reserved
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SIST EN ISO 660:2009
ISO 660:2009(E)
Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies

(ISO member bodies). The work of preparing International Standards is normally carried out through ISO

technical committees. Each member body interested in a subject for which a technical committee has been

established has the right to be represented on that committee. International organizations, governmental and

non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the

International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International Standards

adopted by the technical committees are circulated to the member bodies for voting. Publication as an

International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent

rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 660 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and

vegetable fats and oils.

This third edition cancels and replaces the second edition (ISO 660:1996), which has been technically revised.

It also incorporates the Amendment ISO 660:1996/Amd.1:2003.
© ISO 2009 – All rights reserved iii
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SIST EN ISO 660:2009
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SIST EN ISO 660:2009
INTERNATIONAL STANDARD ISO 660:2009(E)
Animal and vegetable fats and oils — Determination of acid
value and acidity
1 Scope

This International Standard specifies three methods (two titrimetric and one potentiometric) for the

determination of the acidity in animal and vegetable fats and oils, hereinafter referred to as fats. The acidity is

expressed preferably as acid value, or alternatively as acidity calculated conventionally.

This International Standard is applicable to refined and crude vegetable or animal fats and oils, soap stock

fatty acids or technical fatty acids. The methods are not applicable to waxes.

Since the methods are completely non-specific, they cannot be used to differentiate between mineral acids,

free fatty acids, and other organic acids. The acid value, therefore, also includes any mineral acids that may

be present.
2 Normative references

The following referenced documents are indispensable for the application of this document. For dated

references, only the edition cited applies. For undated references, the latest edition of the referenced

document applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
acid value

number of milligrams of potassium hydroxide required to neutralize the free fatty acids present in 1 g of fat,

when determined in accordance with the procedure specified in this International Standard

NOTE The acid value is expressed in milligrams per gram.
3.2
acidity

content of free fatty acids determined according to the procedure specified in this International Standard

NOTE The acidity is expressed as a percentage by mass. If the result of the determination is reported as acidity

without further explanation, this is, by convention, the acidity based on the oleic acid content.

© ISO 2009 – All rights reserved 1
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SIST EN ISO 660:2009
ISO 660:2009(E)
4 Principle

The sample is dissolved in a suitable solvent mixture, and the acids present are titrated with an ethanolic or

methanolic solution of potassium or sodium hydroxide.
The methods specified in 9.1 and 9.2 are reference methods.
5 Reagents

WARNING — Attention is drawn to the regulations which specify the handling of hazardous

substances. Technical, organizational and personal safety measures shall be followed.

Use only reagents of recognized analytical grade, unless otherwise specified.
5.1 Solvent A for solvent mixture (5.3): ethanol, volume fraction, ϕ ≈ 96 %.
As a replacement, propan-2-ol, volume fraction, ϕ ≈ 99 %, can be used.
5.2 Solvent B for solvent mixture (5.3): diethyl ether, peroxide-free.

As a replacement, tert-butyl methyl ether, light petroleum (boiling range 40 °C to 60 °C) or toluene can be

used.

WARNING — Diethyl ether is very flammable and may form explosive peroxides. Use with great

caution.

5.3 Solvent mixture, mix equal volumes of solvent A and B, (e.g. ϕ = 50 ml/100 ml and

ϕ = 50 ml/100 ml).

For hard or animal fats, a solvent mixture of one volume of solvent A (e.g. 25 ml) and three volumes of tert-

butyl methyl ether or toluene (e.g. 75 ml) is recommended.

Neutralize, just before use, by adding potassium hydroxide solution in the presence of 0,3 ml of the

phenolphthalein solution per 100 ml of solvent mixture.
For the titration with aqueous KOH, the solvent propan-2-ol can be used.
5.4 Ethanol or methanol, of minimum volume fraction, ϕ = 95 %.

5.5 Sodium hydroxide or potassium hydroxide, ethanolic or methanolic standard volumetric solutions,

amount of substance concentration c(NaOH) or c(KOH) = 0,1 mol/l and 0,5 mol/l. The concentration shall be

checked with a standard volumetric HCl solution.

NOTE The ethanolic/methanolic sodium/potassium hydroxide solution may be replaced by an aqueous

sodium/potassium hydroxide solution, but only if the volume of water introduced does not lead to phase separation.

5.6 Phenolphthalein, solution in ethanol, mass concentration, ρ = 1 g/100 ml.
5.7
...

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