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SIST EN 14526:2005

(Main)

Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using pre-column derivatization with peroxide or periodate oxidation

Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using pre-column derivatization with peroxide or periodate oxidation

This European standard specifies a method for the quantitative determination of saxitoxin (STX) and decarbamoyl saxitoxin (dc-STX) in mussels. It may also be applicable in other shellfish, for example scallops. The limit of determination of this method (signal/noise = 10) is 0,006 mg/kg for saxitoxin and 0,02 mg/kg for dc-saxitoxin in mussel meat. The method has been tested for saxitoxin at levels at 0,4 mg/kg and 0,5 mg/kg and for dc-saxitoxin at levels at 0,4 mg/kg and 1,6 mg/kg.

Lebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxin in Muscheln - HPLC-Verfahren mit Vorsäulenderivatisierung mit Peroxid- oder Periodatoxdation

Dieses Dokument legt ein Verfahren für die quantitative Bestimmung von Saxitoxin (STX) und Decarbamoyl-Saxitoxin (DCSTX) in Miesmuscheln fest. Es darf auch auf andere Muscheln angewendet werden, z. B. Jacobsmuscheln. Die Bestimmungsgrenze dieses Verfahrens (Signal/Rauschverhältnis = 10) ist 0,006 mg/kg für Saxitoxin und 0,02 mg/kg für DC-Saxitoxin in Muschelfleisch. Dieses Verfahren wurde im Ringversuch für Saxitoxingehalte von 0,4 mg/kg und 0,5 mg/kg und für DC-Saxitoxingehalte von 0,4 mg/kg und 1,6 mg/kg geprüft.

Produits alimentaires - Détermination de la teneur en saxitoxine et en dc-saxitoxine dans les moules - Méthode par CLHP avec dérivation pré-colonne et par oxydation au peroxide ou au periodate

Le présent document spécifie une méthode de détermination quantitative de la saxitoxine (STX) et de la décarbamoyle saxitoxine (dc-STX) dans les moules. Cette méthode peut également s'appliquer a d'autres mollusques, par exemple les coquilles St-Jacques. La limite de quantification de cette méthode (signal/bruit = 10) est de 0,006 mg/kg pour la saxitoxine et de 0,02 mg/kg pour la dc-saxitoxine dans la chair de moule. Cette méthode a été évaluée pour la saxitoxine aux teneurs de 0,4 mg/kg et 0,5 mg/kg et pour la dc-saxitoxine aux teneurs de 0,4 mg/kg et 1,6 mg/kg.

Živila - Določevanje saksitoksina in dc-saksitoksina v školjkah - Metoda HPLC z uporabo predkolonske derivatizacije s peroksidno ali perjodatno oksidacijo

General Information

Status
Withdrawn
Publication Date
31-Dec-2004
Withdrawal Date
19-Feb-2017
ICS
67.120.30 - Fish and fishery products
Technical Committee
KŽP - Agricultural food products
Current Stage
9900 - Withdrawal (Adopted Project)
Start Date
20-Feb-2017
Due Date
15-Mar-2017
Completion Date
20-Feb-2017
Ref Project
EN 14526:2004 - Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using pre-column derivatization with peroxide or periodate oxidation

Relations

Replaced By
SIST EN 14526:2017 - Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation
Effective Date
08-Jun-2022
Replaced By
SIST EN 14526:2017 - Foodstuffs - Determination of saxitoxin-group toxins in shellfish - HPLC method using pre-column derivatization with peroxide or periodate oxidation
Effective Date
01-Mar-2017

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.NVLGDFLMRLebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxin in Muscheln - HPLC-Verfahren mit Vorsäulenderivatisierung mit Peroxid- oder PeriodatoxdationProduits alimentaires - Détermination de la teneur en saxitoxine et en dc-saxitoxine dans les moules - Méthode par CLHP avec dérivation pré-colonne et par oxydation au peroxide ou au periodateFoodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using pre-column derivatization with peroxide or periodate oxidation67.120.30Ribe in ribji proizvodiFish and fishery productsICS:Ta slovenski standard je istoveten z:EN 14526:2004SIST EN 14526:2005en01-januar-2005SIST EN 14526:2005SLOVENSKI
STANDARD



SIST EN 14526:2005



EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14526August 2004ICS 67.120.30English versionFoodstuffs - Determination of saxitoxin and dc-saxitoxin inmussels - HPLC method using pre-column derivatization withperoxide or periodate oxidationProduits alimentaires - Détermination de la teneur ensaxitoxine et en dc-saxitoxine dans les moules - Méthodepar CLHP avec dérivation pré-colonne et par oxydation auperoxide ou au periodateLebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxinin Muscheln - HPLC-Verfahren mitVorsäulenderivatisierung mit Peroxid- oderPeriodatoxdationThis European Standard was approved by CEN on 21 May 2004.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2004 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14526:2004: ESIST EN 14526:2005



EN 14526:2004 (E) 2 Contents Page Foreword.3 Introduction.4 1 Scope.5 2 Normative references.5 3 Principle.5 4 Reagents.6 5 Apparatus.9 6 Procedure.11 7 HPLC determination.13 8 Calibration graph.13 9 Identification.14 10 Calculation.14 11 Precision.14 12 Test report.15 Annex A (informative)
Performance data.
...

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