SIST EN 17697:2023

Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli, Pediococci, Enterococci and Bacilli in animal feeds

This document defines a Pulsed Field Gel Electrophoresis (PFGE) methodology for the identification of authorized probiotic Lactobacillus, Pediococcus, Enterococcus and Bacillus strains. The method can be applied to purified colonies obtained from cultured premixtures and feeds, in order to verify the presence of strains used as feed additives in declared concentrations, even against eventual microbial background resulting from nonsterile matrices.

Futtermittel: Probenahme- und Untersuchungsverfahren - PFGE Typisierung von Laktobazillen, Pediokokken, Enterokokken und Bazillen in Futtermitteln

Dieses Dokument legt eine Methodik der Pulsfeld-Gelelektrophorese (PFGE) zur Identifizierung zugelassener probiotischer Lactobacillus-, Pediococcus-, Enterococcus- und Bacillus-Stämme fest. Das Verfahren kann auf gereinigte Kolonien angewendet werden, die aus kultivierten Vormischungen und Futtermitteln gewonnen werden, um das Vorhandensein von Stämmen, die als Futtermittelzusatzstoffe in deklarierten Konzentrationen verwendet werden, sogar vor einem möglichen mikrobiellen Hintergrund, der aus nicht sterilen Matrices resultiert.

Aliments des animaux : Méthodes d’analyse - Typage EGCP des lactobacilles, pédiocoques, entérocoques et bacilles dans les aliments des animaux

Le présent document définit une technique d’électrophorèse en champ pulsé (EGCP) pour identifier les souches probiotiques autorisées de lactobacilles, pédiocoques, entérocoques et bacilles. La méthode peut être appliquée aux colonies purifiées obtenues à partir de prémélanges et aliments cultivés, pour vérifier la présence de souches utilisées comme additifs alimentaires dans des concentrations déclarées, même contre l’éventuel microbiote annexe résultant de matrices non stériles.

Krma - Metode vzorčenja in analize - Tipizacija laktobacilov, pediokokov, enterokokov in bacilov z metodo PFGE v krmi

Ta dokument določa metodologijo PFGE za identifikacijo odobrenih probiotičnih sevov laktobacilov, pediokokov, enterokokov in bacilov. To metodo je mogoče uporabljati za prečiščene kolonije, pridobljene iz kultiviranih premiksov in krme, da se potrdi prisotnost sevov, ki se uporabljajo kot krmni dodatki v prijavljenih koncentracijah, tudi glede na morebitno mikrobno ozadje, ki je posledica nesterilnih matric.

General Information

• Status: Published
• Public Enquiry End Date: 21-Oct-2021
• Publication Date: 17-Aug-2023
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 20-Jun-2023
• Due Date: 25-Aug-2023
• Completion Date: 18-Aug-2023
• Mandate: M/521 - Mandate for standardisation addressed to CEN for methods of analysis in the field of animal nutrition - part 1
• Ref Project: CEN/TS 17697:2023 - Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli, Pediococci, Enterococci and Bacilli in animal feeds

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17697:2023
01-september-2023
Krma - Metode vzorčenja in analize - Tipizacija laktobacilov, pediokokov,
enterokokov in bacilov z metodo PFGE v krmi
Animal feeding stuffs - Methods of sampling and analysis - PFGE typing of Lactobacilli,
Pediococci, Enterococci and Bacilli in animal feeds
Futtermittel: Probenahme- und Untersuchungsverfahren - PFGE Typisierung von
Laktobazillen, Pediokokken, Enterokokken und Bazillen in Futtermitteln
Aliments des animaux : Méthodes d’analyse - Typage EGCP des lactobacilles,
pédiocoques, entérocoques et bacilles dans les aliments des animaux
Ta slovenski standard je istoveten z: CEN/TS 17697:2023
ICS:
65.120 Krmila Animal feeding stuffs
SIST EN 17697:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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CEN/TS 17697
TECHNICAL SPECIFICATION

SPÉCIFICATION TECHNIQUE

June 2023
TECHNISCHE SPEZIFIKATION
ICS 65.120
English Version

Animal feeding stuffs - Methods of sampling and analysis -
PFGE typing of Lactobacilli, Pediococci, Enterococci and
Bacilli in animal feeds
Aliments des animaux : Méthodes d'analyse - Typage Futtermittel: Probenahme- und
EGCP des lactobacilles, pédiocoques, entérocoques et Untersuchungsverfahren - PFGE Typisierung von
bacilles dans les aliments des animaux Laktobazillen, Pediokokken, Enterokokken und
Bazillen in Futtermitteln
This Technical Specification (CEN/TS) was approved by CEN on 10 March 2023 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to
submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS
available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in
parallel to the CEN/TS) until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 17697:2023 E
worldwide for CEN national Members.

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SIST EN 17697:2023
CEN/TS 17697:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Reagents . 6
6 Media . 8
7 Apparatus . 9
8 Procedure . 10
9 Test report .
...

SLOVENSKI STANDARD
oSIST prEN 17697:2021
01-oktober-2021
Krma: Metode analize - Tipizacija laktobacilov, pediokokov, enterokokov in bacilov
z metodo PFGE v krmi
Animal feeding stuffs: Methods of analysis - PFGE typing of Lactobacilli, Pediococci,
Enterococci and Bacilli in animal feeds
Futtermittel: Probenahme- und Untersuchungsverfahren - PFGE Typisierung von
Laktobazillen, Pediokokken, Enterokokken und Bazillen
Aliments des animaux : Méthodes d’analyse - Typage EGCP des lactobacilles,
pédiocoques, entérocoques et bacilles dans les aliments des animaux
Ta slovenski standard je istoveten z: prEN 17697
ICS:
65.120 Krmila Animal feeding stuffs
oSIST prEN 17697:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17697:2021

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oSIST prEN 17697:2021


DRAFT
EUROPEAN STANDARD
prEN 17697
NORME EUROPÉENNE

EUROPÄISCHE NORM

August 2021
ICS 65.120
English Version

Animal feeding stuffs: Methods of analysis - PFGE typing of
Lactobacilli, Pediococci, Enterococci and Bacilli in animal
feeds
Aliments des animaux : Méthodes d'analyse - Typage Futtermittel: Probenahme- und
EGCP des lactobacilles, pédiocoques, entérocoques et Untersuchungsverfahren - PFGE Typisierung von
bacilles dans les aliments des animaux Laktobazillen, Pediokokken, Enterokokken und
Bazillen
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 327.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17697:2021 E
worldwide for CEN national Members.

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oSIST prEN 17697:2021
prEN 17697:2021 (E)
Contents Page
European foreword . 4
Introduction . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 6
4 Principle . 7
5 Reagents . 7
5.1 Tris (hydroxymet yl) aminomethane hydrochloride (Tris-HCl) . 7
5.2 Ethylenediaminetetraacetic acid (EDTA) . 7
5.3 TE-buffer: TE 10 mM Tris-Hcl, 1 mM EDTA, adjust to pH 8,0 and autoclave . 7
5.4 Lysozyme (≥20 000 U/mg).
...