Textile fabrics - Determination of antibacterial activity - Agar diffusion plate test (ISO 20645:2004)

This standard specifies a method for investigating the effect of textile auxiliaries on rottability of cellulose containing textiles.

Textile Flächengebilde - Prüfung der antibakteriellen Wirkung - Agarplattendiffusionstest (ISO 20645:2004)

Dieses Dokument legt ein Prüfverfahren zur Bestimmung der Wirkung antibakterieller Behandlungen von
Geweben, Gewirken und anderen flachen Textilien fest.
Dieses Verfahren gilt für die Prüfung hygienischer Ausrüstungen hydrophiler luftdurchlässiger Materialien oder
antibakteriell ausgerüsteter Fasern. Zur Prüfung nach diesem Verfahren ist eine Mindestdiffusion der
antimikrobiellen Mittel in den Prüfagar erforderlich.
ANMERKUNG Die Prüfung anderer Materialien nach diesem Verfahren ist möglich, sofern es dementsprechend
angepasst wird.
Das Prüfverfahren ist nicht geeignet für die Prüfung von Textilien, deren antibakterielle Behandlungen mit dem
Agar eine Reaktion eingehen.

Etoffes - Contrôle de l'activité antibactérienne - Essai de diffusion sur plaques de géloses (ISO 20645:2004)

La présente norme fixe une méthode de détermination de l'activité antibactérienne des traitements antimicrobiens appliqués aux textiles tissés, tricotés et autres textiles plats.
La présente méthode s'applique aux essais effectués sur les apprets hygiéniques des matériaux hydrophiles perméables a l'air. Le présent mode opératoire nécessite une diffusion minimale de l'appret antimicrobien dans la gélose.
NOTE   La présente méthode peut convenir a d'autres matériaux a condition d'etre adaptée en conséquence.
La présente méthode n'est pas appropriée aux essais sur textiles traités avec des apprets antimicrobiens qui réagissent avec la gélose.

Ploskovne tekstilije - Določitev protibakterijskega delovanja - Preskus na plošči, prekriti z agarjem (ISO 20645:2004)

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2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Textile Flächengebilde - Prüfung der antibakteriellen Wirkung - Agarplattendiffusionstest (ISO 20645:2004)Etoffes - Contrôle de l'activité antibactérienne - Essai de diffusion sur plaques de géloses (ISO 20645:2004)Textile fabrics - Determination of antibacterial activity - Agar diffusion plate test (ISO 20645:2004)59.080.30TkanineTextile fabrics07.100.99Drugi standardi v zvezi z mikrobiologijoOther standards related to microbiologyICS:Ta slovenski standard je istoveten z:EN ISO 20645:2004SIST EN ISO 20645:2005en01-marec-2005SIST EN ISO 20645:2005SLOVENSKI

SIST EN ISO 20645:2005

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN ISO 20645December 2004ICS 59.080.30English versionTextile fabrics - Determination of antibacterial activity - Agardiffusion plate test (ISO 20645:2004)Etoffes - Contrôle de l'activité antibactérienne - Essai dediffusion sur plaques de gélose (ISO 20645:2004)Textile Flächengebilde - Prüfung der antibakteriellenWirkung - Agarplattendiffusionstest (ISO 20645:2004)This European Standard was approved by CEN on 2 September 2004.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2004 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 20645:2004: ESIST EN ISO 20645:2005

EN ISO 20645:2004 (E) 2 Contents Page Foreword.3 Introduction.4 1 Scope.5 2 Terms and definitions.5 3 Safety precautions.5 4 Principle.5 5 Apparatus, reagents and culture media.5 5.1 Apparatus.5 5.2 Reagents and culture media.6 6 Test bacteria.7 7 Preparation of the bacteria cultures.7 7.1 General.7 7.2 Culturing with lyophilized bacteria.7 7.3 Culturing from the agar.7 8 Preparation of test specimens.8 8.1 General.8 8.2 Preparation of the test material.8 8.3 Material for blind tests.8 8.4 Conditioning of specimens.8 9 Test procedure.8 10 Assessment of the tests.9 11 Evaluation.10 12 Test report.10 Annex A (informative)
Special specimens and tests.11 Annex B (informative)
European Suppliers of ATCC.12 Bibliography.13
SIST EN ISO 20645:2005

EN ISO 20645:2004 (E) 3 Foreword This document (EN ISO 20645:2004) has been prepared by Technical Committee CEN/TC 248 “Textiles and textile products”, the secretariat of which is held by BSI, in collaboration with Technical Committee ISO/TC 38 “Textiles”. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2005, and conflicting national standards shall be withdrawn at the latest by June 2005. This document includes a Bibliography. CAUTION — This method involves the use of processes that could lead to a hazardous situation. Attention is drawn to the safety precautions in Clause 3. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
SIST EN ISO 20645:2005

EN ISO 20645:2004 (E) 4 Introduction The application of an antimicrobial finish to a textile can prevent bacterial growth and might reduce the effects of microbial pathway products, biodeterioration and microbiogenous odours. This method determines the activity of such treatments qualitatively when different products are compared. Semi-quantitative information on the effect of treatments can be obtained when different concentrations of the same product are compared. SIST EN ISO 20645:2005

EN ISO 20645:2004 (E) 5 1 Scope This document specifies a method for the determination of the effect of antibacterial treatments applied to woven, knitted and other flat textiles.
This method is applicable to testing hygienic finishes of hydrophilic, air-permeable materials or antibacterial products incorporated in the fibre. A minimum diffusion of the antibacterial treatment into the test agar is necessary with this procedure.
NOTE Other materials may be tested using this method, provided that it is adapted accordingly. This method is not suitable for testing textiles treated with antibacterial treatments that react with the agar. 2 Terms and definitions For the purposes of this document, the following term and definition applies. antibacterial effect inhibition of bacterial growth in favourable growing conditions 3 Safety precautions This method requires the use of bacteria and conditions that promote bacterial growth. Since the bacteria might be pathogenic the tests should be carried out by trained personnel. Appropriate safety precautions should be observed. 4 Principle Specimens of the material to be tested are placed on two-layer agar plates. The lower layer consists of a culture medium free from bacteria and the upper layer is inoculated with the selected bacteria. The textiles are tested on both sides. The level of antibacterial activity is assessed by examining the extent of bacterial growth in the contact zone between the agar and the specimen and, if present, the extent of the inhibition zone around the specimen. 5 Apparatus, reagents and culture media
5.1 Apparatus 5.1.1 Incubator , capable of maintaining a temperature of (37 ± 1)°C. 5.1.2 Autoclave, capable of operating at 121°C and 205 kPa (2,05 bar).
5.1.3 Water bath, capable of maintaining a temperature of (45 ± 2)°C. 5.1.4 Shaker, for test tubes
5.1.5 Microscope, 20 × magnification, lighting from beneath (lens 20x, stereomicroscope 20x). 5.1.6 Petri dishes, of glass or polystyrene construction and 9 cm inner diameter SIST EN ISO 20645:2005

EN ISO 20645:2004 (E) 6 5.2 Reagents and culture media 5.2.1 Reagents Use only reagents of recognized analytical grade and distilled water or water of equivalent purity. 5.2.2 Culture media Dry agar, available commercially with the following composition1. Should commercially available agars be unsuitable for the bacteria to be tested, the culture media shall be adapted or replaced accordingly. Such changes shall be mentioned in the test report Composition of the nutrient broth for test strains: trypton peptone 17 g phyton peptone 3 g sodium chloride 5 g di-potassium
hydrogen phosphate
2,5 g dextrose 2,5 g distilled water 1 000 ml Preparation Prepare the nutrient broth by heating the above solids in water until they are completely dissolved. Sterilize the broth at 121 °C (205 kPa) in the autoclave for 15 min. After sterilization, the pH of the broth shall be 7,3 ±
0,1 at 20 °C.
Composition of the medium for tests: trypton peptone 15 g phyton peptone 5

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