SIST EN ISO 14675:2003

Milk and milk products - Guidelines for a standardized description of competitive enzyme immunoassays - Determination of aflatoxin M1 content (ISO 14675:2003)

ISO 14675|IDF186:2002 give guidelines on the use of screening methods used for the determination of aflatoxin M1 content in milk and milk products, based upon competitive enzyme immuno-assays.
For legal purposes, positive enzyme immunoassay results require confirmation by an accepted reference method. However, depending on whether the test complies with the specifications given hereafter, enzyme immunoassays can be used for routine quality control, especially when the absence of aflatoxin M1 above the regulatory limit needs to be documented.

Milch und Milchprodukte - Leitfaden für eine vereinheitlichte Beschreibung kompetitiver Enzym-Immunoassays - Bestimmung des Gehalts an Aflatoxin M1 (ISO 14675:2003)

Diese Internationale Norm legt einen Leitfaden für die Anwendung von Screeningverfahren, die zur Bestimmung des Gehalts an Aflatoxin M1 in Milch und Milchprodukten angewendet werden und deren Grundlage kompetitive Enzym-Immunoassays sind, fest.
Zur Erfüllung gesetzlicher Auflagen ist bei den Ergebnissen kompetitiver Enzym-Immunoassays die Bestätigung durch ein anerkanntes Referenzverfahren erforderlich. Kompetitive Enzym-Immunoassays können jedoch (abhängig davon, ob der Test den im Weiteren angegebenen Spezifikationen entspricht) für die Routine-Qualitätskontrolle verwendet werden, besonders wenn das Nichtvorhandensein von Rückständen von Aflatoxin M1 oberhalb des durch Bestimmungen festgelegten Grenzwerts nachzuweisen ist.

Lait et produits laitiers - Lignes directrices pour une description normalisée des tests immuno-enzymatiques - Détermination de la teneur en aflatoxine M1 (ISO 14675:2003)

L'ISO 14675|IDF 186:2002 donne des lignes directrices concernant l'utilisation des méthodes de criblage pour le dosage de l'aflatoxine M1 dans le lait et les produits laitiers, basées sur des tests immuno-enzymatiques.
Pour des raisons légales, les résultats de tests immuno-enzymatiques positifs nécessitent une confirmation par une méthode de référence généralement admise. Cependant, dans la mesure où ils répondent aux spécifications indiquées ci-après, les tests immuno-enzymatiques peuvent être utilisés pour des contrôles qualité de routine, surtout si l'absence d'aflatoxine M1 au-delà de la limite réglementaire doit être confirmée.

Mleko in mlečni izdelki - Vodila za standardiziran opis encimsko-imunoloških preskusov - Določitev aflatoxina M1 (ISO 14675:2003)

General Information

• Status: Published
• Publication Date: 31-Oct-2003
• ICS: 67.100.10 - Milk and processed milk products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-Nov-2003
• Due Date: 01-Nov-2003
• Completion Date: 01-Nov-2003
• Ref Project: EN ISO 14675:2003 - Milk and milk products - Guidelines for a standardized description of competitive enzyme immunoassays - Determination of aflatoxin M1 content (ISO 14675:2003)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 14675:2003
01-november-2003
0OHNRLQPOHþQLL]GHONL9RGLOD]DVWDQGDUGL]LUDQRSLVHQFLPVNRLPXQRORãNLK
SUHVNXVRY'RORþLWHYDIODWR[LQD0,62
Milk and milk products - Guidelines for a standardized description of competitive enzyme
immunoassays - Determination of aflatoxin M1 content (ISO 14675:2003)
Milch und Milchprodukte - Leitfaden für eine vereinheitlichte Beschreibung kompetitiver
Enzym-Immunoassays - Bestimmung des Gehalts an Aflatoxin M1 (ISO 14675:2003)
Lait et produits laitiers - Lignes directrices pour une description normalisée des tests
immuno-enzymatiques - Détermination de la teneur en aflatoxine M1 (ISO 14675:2003)
Ta slovenski standard je istoveten z: EN ISO 14675:2003
ICS:
67.100.10 0OHNRLQSUHGHODQLPOHþQL Milk and processed milk
SURL]YRGL products
SIST EN ISO 14675:2003 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 14675:2003

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SIST EN ISO 14675:2003
EUROPEAN STANDARD
EN ISO 14675
NORME EUROPÉENNE
EUROPÄISCHE NORM
January 2003
ICS 67.100.10
English version
Milk and milk products - Guidelines for a standardized
description of competitive enzyme immunoassays -
Determination of aflatoxin M1 content (ISO 14675:2003)
Lait et produits laitiers - Lignes directrices pour une Milch und Milchprodukte - Leitfaden für eine vereinheitlichte
description normalisée des tests immuno-enzymatiques - Beschreibung kompetitiver Enzym-Immunoassays -
Détermination de la teneur en aflatoxine M1 (ISO Bestimmung des Gehalts an Aflatoxin M1 (ISO
14675:2003) 14675:2003)
This European Standard was approved by CEN on 6 December 2002.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2003 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14675:2003 E
worldwide for CEN national Members.

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SIST EN ISO 14675:2003
EN ISO 14675:2003 (E)
CORRECTED 2003-04-30
Foreword
This document (EN ISO 14675:2003) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 302 "Milk and
milk products - Methods of sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by July 2003, and conflicting national standards
shall be withdrawn at the latest by July 2003.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium, Czech
Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,
Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and
the United Kingdom.
Endorsement notice
The text of ISO 14675:2003 has been approved by CEN as EN ISO 14675:2003 without any
modifications.
2

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SIST EN ISO 14675:2003



INTERNATIONAL ISO
STANDARD 14675
IDF
186
First edition
2003-01-15


Milk and milk products — Guidelines for a
standardized description of competitive
enzyme immunoassays — Determination of
aflatoxin M content
1
Lait et produits laitiers — Lignes directrices pour une description
normalisée des tests immuno-enzymatiques — Détermination de la teneur
en aflatoxine M
1




Reference numbers
ISO 14675:2003(E)
IDF 186:2003(E)
©
 ISO and IDF 2003

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SIST EN ISO 14675:2003
ISO 14675:2003(E)
IDF 186:2003(E)
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ii © ISO and IDF 2003 – All rights reserved

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SIST EN ISO 14675:2003
ISO 14675:2003(E)
IDF 186:2003(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted
by the technical committees are circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 14675IDF 186 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5, Milk
and milk products, and the International Dairy Federation (IDF), in collaboration with AOAC International. It is being
published jointly by ISO and IDF and separately by AOAC International.
© ISO and IDF 2003 – All rights reserved iii

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SIST EN ISO 14675:2003
ISO 14675:2003(E)
IDF 186:2003(E)
Foreword
IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Committee in
every member country. Every National Committee has the right to be represented on the IDF Standing Committees
carrying out the technical work. IDF collaborates with ISO and AOAC International in the development of standard
methods of analysis and sampling for milk and milk products.
Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National
Committees for voting. Publication as an International Standard requires approval by at least 50 % of National
Committees casting a vote.
International Standard ISO 14675IDF 186 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
All work was carried out by the Joint ISO/IDF/AOAC Action Team, Organic contaminants, of the Standing
Committee on Analytical methods for additives and contaminants, under the aegis of its project leader,
Dr E. Märtlbauer (DE).

iv © ISO and IDF 2003 – All rights reserved

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SIST EN ISO 14675:2003
ISO 14675:2003(E)
IDF 186:2003(E)
Introduction
Proprietary methods such as ELISA methods cannot be described in separate International Standards. Therefore,
this International Standard is intended to provide guidelines on basic parameters required for evaluation/validation
of competitive enzyme immunoassays for the quantitative determination of aflatoxin M in milk and milk products.
1
Currently several quantitative immunochemical test formats are commercially available, which all share the basic
principles of the competitive enzyme immunoassay. However, since the test format of the 96-well microtitre plate
assay is most commonly used for quantitative measurement purposes, the parameters given in this International
Standard are specifically adopted to this test format, and may not necessarily apply in full to a different test format.

© ISO and IDF 2003 – All rights reserved v

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SIST EN ISO 14675:2003

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SIST EN ISO 14675:2003
ISO 14675:2003(E)
INTERNATIONAL STANDARD
IDF 186:2003(E)

Milk and milk products — Guidelines for a standardized
description of competitive enzyme immunoassays —
Determination of aflatoxin M content
1
1 Scope
This International Standard give guidelines on the use of screening methods used for the determination of aflatoxin
M content in milk and milk products, based upon competitive enzyme immunoassays.
1
For legal purpos
...