SIST EN ISO 15774:2017

Animal and vegetable fats and oils - Determination of cadmium content by direct graphite furnace atomic absorption spectrometry (ISO 15774:2017)

ISO 15774:2017 describes a method for the determination of trace amounts (micrograms per kilogram) of cadmium in all types of crude or refined edible oils and fats.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Tierische und pflanzliche Fette und Öle - Bestimmung von Cadmium mit Direkt-Graphitofen-Atomabsorptionsspektrometrie (ISO 15774:2017)

Corps gras d'origines animale et végétale - Détermination de la teneur en cadmium par spectrométrie d'absorption atomique à four graphite (ISO 15774:2017)

ISO 15774:2017 décrit une méthode de détermination des traces de cadmium (microgrammes par kilogramme) dans tous les types de corps gras alimentaires, qu'ils soient raffinés ou bruts.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application du présent document.

Rastlinske in živalske maščobe in olja - Določevanje kadmija neposredno z grafitno pečjo z atomsko absorpcijsko spektrometrijo (ISO 15774:2017)

Ta dokument opisuje metodo za določevanje sledi (mikrogrami na kilogram) kadmija v vseh vrstah surovih ali rafiniranih užitnih olj in maščob.
Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem dokumentu.

General Information

• Status: Published
• Public Enquiry End Date: 19-Dec-2016
• Publication Date: 06-Apr-2017
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 14-Mar-2017
• Due Date: 19-May-2017
• Completion Date: 07-Apr-2017
• Ref Project: EN ISO 15774:2017 - Animal and vegetable fats and oils - Determination of cadmium content by direct graphite furnace atomic absorption spectrometry (ISO 15774:2017)

Relations

• Replaces: SIST EN ISO 15774:2001 - Animal and vegetable fats and oils - Determination of cadmium content by direct graphite furnace atomic absorption spectrometry (ISO 15774:2000)
• Effective Date: 01-May-2017

Overview

EN ISO 15774:2017 - "Animal and vegetable fats and oils - Determination of cadmium content by direct graphite furnace atomic absorption spectrometry" - is a CEN-adopted ISO method for measuring trace cadmium (Cd) at micrograms per kilogram in crude and refined edible oils and fats. Published in 2017, this method supersedes EN ISO 15774:2001. Milk and milk‑derived fats are excluded from the scope.

Key topics and technical requirements

• Analytical principle: direct graphite furnace atomic absorption spectrometry (GF‑AAS). Samples are incinerated/atomized in a pyrolytic graphite tube and Cd is measured at 228.8 nm.
• Matrix modification: palladium is used as a matrix modifier to prevent Cd loss during thermal pretreatment (0.1% Pd solution prepared from PdCl2).
• Calibration and standards: use organometallic cadmium standard (example given) to prepare a 10 mg/kg stock and working standards (2.5, 5.0 and 10.0 µg/kg) in refined vegetable oil.
• Reagents & gases: analytical‑grade water (ISO 3696 grade 1), cyclohexane, hydrochloric acid, PdCl2, and argon (≥99.99%) for the furnace.
• Apparatus: GF‑AAS with peak‑area/autocalibrate modes, deuterium background or Zeeman correction; uncoated graphite tubes with a pyrolytic platform; optional autosampler (sample cup heating at 60 °C ±10 °C).
• Sample preparation: oils and standards are equilibrated at ~60 °C; high‑melting fats (mp ≥ 40 °C) are diluted 1:1 (mass/mass) with blank oil; autosampler injection volumes specified (15 µl heated, 20 µl when solvent‑diluted).
• Quality & performance: document includes precision data (repeatability, reproducibility) from interlaboratory tests and prescribes reporting elements for test reports.

Applications

• Routine food safety testing for cadmium contamination in edible oils and fats.
• Quality control in edible oil refining, seed oil processing, biodiesel feedstock assessment (when relevant to food chain).
• Regulatory compliance testing for maximum Cd limits set by food safety authorities.
• Method validation and interlaboratory comparison studies for trace metal analysis in lipid matrices.

Who uses this standard

• Accredited analytical and food testing laboratories
• Oil & fat producers and refiners (quality assurance teams)
• Regulatory agencies and public health laboratories
• Method developers and instrument vendors supplying GF‑AAS solutions

Related standards

• ISO 3696 - Water for analytical laboratory use (normative reference).
• EN ISO 15774:2001 - previous edition (now superseded).

Keywords: EN ISO 15774:2017, cadmium determination, graphite furnace atomic absorption spectrometry, GF‑AAS, edible oils, fats, trace metals, palladium matrix modifier, food safety.

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2017
1DGRPHãþD
SIST EN ISO 15774:2001
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHNDGPLMDQHSRVUHGQR]
JUDILWQRSHþMR]DWRPVNRDEVRUSFLMVNRVSHNWURPHWULMR,62
Animal and vegetable fats and oils - Determination of cadmium content by direct graphite
furnace atomic absorption spectrometry (ISO 15774:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung von Cadmium mit Direkt-
Graphitofen-Atomabsorptionsspektrometrie (ISO 15774:2017)
Corps gras d'origines animale et végétale - Détermination de la teneur en cadmium par
spectrométrie d'absorption atomique à four graphite (ISO 15774:2017)
Ta slovenski standard je istoveten z: EN ISO 15774:2017
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 15774
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2017
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 15774:2001
English Version
Animal and vegetable fats and oils - Determination of
cadmium content by direct graphite furnace atomic
absorption spectrometry (ISO 15774:2017)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination de la teneur en cadmium par von Cadmium mit Direkt-Graphitofen-
spectrométrie d'absorption atomique à four graphite Atomabsorptionsspektrometrie (ISO 15774:2017)
(ISO 15774:2017)
This European Standard was approved by CEN on 15 February 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15774:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 15774:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2017, and conflicting national standards shall
be withdrawn at the latest by August 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 15774:2001.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 15774:2017 has been approved by CEN as EN ISO 15774:2017 without any modification.
INTERNATIONAL ISO
STANDARD 15774
Second edition
2017-02
Animal and vegetable fats and oils —
Determination of cadmium content
by direct graphite furnace atomic
absorption spectrometry
Corps gras d’origines animale et végétale — Détermination de la
teneur en cadmium par spectrométrie d’absorption atomique à four
graphite
Reference number
ISO 15774:2017(E)
©
ISO 2017
ISO 15774:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
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ii © ISO 2017 – All rights reserved

ISO 15774:2017(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Procedure. 3
7.1 General . 3
7.2 Treatment of samples, vegetable oil and standards . 3
7.3 Preparation of apparatus . 3
7.4 Determination . 4
7.4.1 Graphite tube blank . 4
7.4.2 Vegetable oil . 4
7.4.3 Calibration of apparatus . 4
7.4.4 Oil (liquid) samples . 4
7.4.5 Fat samples with melting point 40 °C and higher . 4
7.4.6 Number of determinations. 5
8 Expression of results . 5
9 Precision . 5
9.1 Interlaboratory test. 5
9.2 Repeatability . 5
9.3 Reproducibility . 5
10 Test report . 5
Annex A (informative) Results of interlaboratory test . 6
ISO 15774:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endor
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