SIST-TS CEN ISO/TS 21268-2:2010

Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter (ISO/TS 21268-2:2007)

This part of ISO/TS 21268 specifies a test providing information on leaching of soil and soil materials under the experimental conditions specified hereafter, and particularly at a liquid to solid ratio of 10 l/kg dry matter. It applies to soil and soil material with a particle size less than or equal to 4 mm. This part of ISO/TS 21268 has been developed to measure the release of inorganic and organic constituents from soil and soil material and the ecotoxicological effects of eluates with respect to micro-organisms, fauna and flora. The test is not suitable for constituents that are volatile under ambient conditions. For ecotoxicological testing, see ISO 15799. The test procedure specified in this part of ISO/TS 21268 produces eluates, which are subsequently characterised by existing physical, chemical and ecotoxicological standard methods. This test is mainly aimed at being used for routine and control purposes, and it cannot be used alone to describe all leaching properties of a soil. Additional leaching tests are needed for that extended goal. This part of ISO/TS 21268 does not address issues related to health and safety. It only determines the leaching properties as outlined in Clause 4.

Bodenbeschaffenheit - Eluierungsverfahren für die anschließende chemische und ökotoxikologische Untersuchung von Boden und von Bodenmaterialien - Teil 2: Schüttelverfahren mit einem Flüssigkeits-/Feststoffverhältnis von 10 l/kg Trockenmasse (ISO/TS 21268-2:2007)

Dieser Teil von ISO/TS 21268 legt eine Prüfung fest, die Informationen zur Auslaugung von Böden und Bodenmaterialien unter den im Weiteren festgelegten experimentellen Bedingungen und insbesondere bei einem Flüssigkeits /Feststoffverhältnis von 10 l/kg Trockenmasse liefert. Er gilt für Böden und Boden-materialien mit einer Korngröße kleiner oder gleich 4 mm.
Dieser Teil von ISO/TS 21268 wurde erstellt, um die Freisetzung anorganischer und organischer Bestandteile aus Böden und Bodenmaterialien sowie die ökotoxikologischen Auswirkungen von Eluaten auf Mikro-organismen, Fauna und Flora zu untersuchen. Die Prüfung ist nicht geeignet für Bestandteile, die unter Umgebungsbedingungen flüchtig sind. Hinsichtlich der ökotoxikologischen Prüfung siehe ISO 15799.
ANMERKUNG 1   Zu den flüchtigen organischen Bestandteilen gehören niedermolekulare Komponenten in Mischungen, wie z. B. Mineralöl.
ANMERKUNG 2   Es ist nicht immer möglich, die Prüfbedingungen gleichzeitig für anorganische und organische Bestandteile zu optimieren und die optimalen Prüfbedingungen können sich auch für unterschiedliche Gruppen von organischen Bestandteilen unterscheiden. Die Prüfanforderungen für organische Bestandteile sind im Allgemeinen strenger als für anorganische Bestandteile. Die Prüfbedingungen, die für die Messung der Freisetzung von organischen Bestandteilen geeignet sind, sind im Allgemeinen auch auf anorganische Bestandteile anwendbar.
ANMERKUNG 3   Für ökotoxikologische Untersuchungen sind Eluate erforderlich, die die Freisetzung sowohl von anorganischen als auch von organischen Schadstoffen widerspiegeln. Im vorliegenden Dokument umfasst die öko-toxikologische Untersuchung auch die gentoxikologische Untersuchung.
Durch das in diesem Teil von ISO/TS 21268 festgelegte Prüfverfahren werden Eluate hergestellt, die anschließend physikalisch, chemisch und ökotoxikologisch nach bestehenden Standardverfahren charakterisiert werden.

Qualité du sol - Modes opératoires de lixiviation en vue d'essais chimiques et écotoxicologiques ultérieurs des sols et matériaux du sol - Partie 2: Essai en bâchée avec un rapport liquide/solide de 10 l/kg de matière sèche (ISO/TS 21268-2:2007)

L'ISO/TS 21268-2:2007 spécifie un essai fournissant des informations sur la lixiviation des sols et des matériaux du sol dans des conditions expérimentales spécifiées, et notamment un rapport liquide/solide égal à 10 l/kg de matière sèche. Elle s'applique à des sols et des matériaux du sol de granularité inférieure ou égale à 4 mm.
L'ISO/TS 21268-2:2007 a été conçue pour étudier le relargage de constituants organiques et inorganiques à partir du sol et des matériaux du sol et les effets écotoxicologiques des éluats vis-à-vis des micro-organismes, de la faune et de la flore. L'essai n'est pas adapté aux espèces qui sont volatiles dans des conditions ambiantes. Pour les essais d'écotoxicité, voir l'ISO 15799.
Le mode opératoire spécifié dans l'ISO/TS 21268-2:2007 permet d'obtenir un éluat qui est ensuite caractérisé par des méthodes physiques, chimiques et écotoxicologiques normalisées.
Cet essai est principalement destiné à être utilisé à des fins de vérification de routine et de contrôle, et il ne peut pas, à lui seul, être utilisé pour déterminer toutes les propriétés de lixiviation d'un sol. D'autres essais sont requis pour atteindre cet objectif plus large. L'ISO/TS 21268-2:2007 ne traite pas des questions liées à la santé et à la sécurité. Elle permet uniquement de déterminer les propriétés de lixiviation.

Kakovost tal - Postopki izluževanja za nadaljnje kemijsko in ekotoksikološko preskušanje tal in talnih (zemeljskih) materialov - 2. del: Šaržni preskus z razmerjem tekoče/trdno 10 l/kg suhe snovi (ISO/TS 21268-2:2007)

Ta del ISO/TS 21268 določa preskus, ki zagotavlja informacije o izluževanju tal in talnih (zemeljskih) materialov pod preskusnimi pogoji, opredeljenimi v nadaljevanju, in zlasti pri razmerju tekoče/trdno 10 l/kg suhe snovi. Velja za tla in talne (zemeljske) materiale z velikostjo delcev, manjših ali enakih 4 mm. Ta del ISO/TS 21268 je bil razvit za merjenje sproščanja neorganskih in organskih sestavin iz tal in talnih (zemeljskih) materialov in ekotoksioloških učinkov izlužkov na mikroorganizme, favno in floro.   Preskus ni primeren za sestavine, ki so hlapne pod okoljskimi pogoji. Za ekotoksiološko preskušanje glej ISO 15799. Preskusni postopek, določen v tem delu ISO/TS 21268, prinaša izlužke, ki so nadalje določeni z obstoječimi fizičnimi, kemičnimi in ekotoksiološkimi standardnimi metodami.  Ta preskus je predvsem namenjen temu, da se uporablja v rutinske in nadzorne namene ter se ne more uporabljati sam za opis vseh lastnosti izluženih tal. Za tak razširjen cilj so potrebni dodatni preskusi izluževanja. Ta del ISO/TS 21268 ne naslavlja vprašanj glede zdravja in varnosti. Kot je navedeno v Klavzuli 4, zgolj določa lastnosti izluženega.

General Information

• Status: Withdrawn
• Publication Date: 19-Jan-2010
• Withdrawal Date: 02-Dec-2019
• ICS: 13.080.05 - Examination of soils in general
• Technical Committee: KAT - Soil quality
• Current Stage: 9900 - Withdrawal (Adopted Project)
• Start Date: 02-Dec-2019
• Due Date: 25-Dec-2019
• Completion Date: 03-Dec-2019
• Ref Project: CEN ISO/TS 21268-2:2009 - Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil materials - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter (ISO/TS 21268-2:2007)

Relations

• Replaced By: SIST EN ISO 21268-2:2020 - Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil-like material - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter (ISO 21268-2:2019)
• Effective Date: 01-Jan-2020
• Replaced By: SIST EN ISO 21268-2:2020 - Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing of soil and soil-like material - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter (ISO 21268-2:2019)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN ISO/TS 21268-2:2010
01-februar-2010
.DNRYRVWWDO3RVWRSNLL]OXåHYDQMD]DQDGDOMQMHNHPLMVNRLQHNRWRNVLNRORãNR
SUHVNXãDQMHWDOLQWDOQLK]HPHOMVNLKPDWHULDORYGHOâDUåQLSUHVNXV]
UD]PHUMHPWHNRþHWUGQRONJVXKHVQRYL,6276
Soil quality - Leaching procedures for subsequent chemical and ecotoxicological testing
of soil and soil materials - Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry
matter (ISO/TS 21268-2:2007)
Bodenbeschaffenheit - Eluierungsverfahren für die anschließende chemische und
ökotoxikologische Untersuchung von Boden und von Bodenmaterialien - Teil 2:
Schüttelverfahren mit einem Flüssigkeits-/Feststoffverhältnis von 10 l/kg Trockenmasse
(ISO/TS 21268-2:2007)
Qualité du sol - Modes opératoires de lixiviation en vue d'essais chimiques et
écotoxicologiques ultérieurs des sols et matériaux du sol - Partie 2: Essai en bâchée
avec un rapport liquide/solide de 10 l/kg de matière sèche (ISO/TS 21268-2:2007)
Ta slovenski standard je istoveten z: CEN ISO/TS 21268-2:2009
ICS:
13.080.05 Preiskava tal na splošno Examination of soils in
general
SIST-TS CEN ISO/TS 21268-2:2010 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST-TS CEN ISO/TS 21268-2:2010

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SIST-TS CEN ISO/TS 21268-2:2010


TECHNICAL SPECIFICATION
CEN ISO/TS 21268-2

SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION
November 2009
ICS 13.080.05
English Version
Soil quality - Leaching procedures for subsequent chemical and
ecotoxicological testing of soil and soil materials - Part 2: Batch
test using a liquid to solid ratio of 10 l/kg dry matter (ISO/TS
21268-2:2007)
Qualité du sol - Modes opératoires de lixiviation en vue Bodenbeschaffenheit - Eluierungsverfahren für die
d'essais chimiques et écotoxicologiques ultérieurs des sols
anschließende chemische und ökotoxikologische
et matériaux du sol - Partie 2: Essai en bâchée avec un Untersuchung von Boden und von Bodenmaterialien - Teil
rapport liquide/solide de 10 l/kg de matière sèche (ISO/TS 2: Schüttelverfahren mit einem Flüssigkeits-
21268-2:2007) -/Feststoffverhältnis von 10 l/kg Trockenmasse (ISO/TS
21268-2:2007)
This Technical Specification (CEN/TS) was approved by CEN on 8 September 2009 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.






EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN ISO/TS 21268-2:2009: E
worldwide for CEN national Members.

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SIST-TS CEN ISO/TS 21268-2:2010
CEN ISO/TS 21268-2:2009 (E)
Contents Page
Foreword .3

2

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SIST-TS CEN ISO/TS 21268-2:2010
CEN ISO/TS 21268-2:2009 (E)
Foreword
The text of ISO/TS 21268-2:2007 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the
International Organization for Standardization (ISO) and has been taken over as CEN ISO/TS 21268-2:2009
by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO/TS 21268-2:2007 has been approved by CEN as a CEN ISO/TS 21268-2:2009 without any
modification.

3

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SIST-TS CEN ISO/TS 21268-2:2010

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SIST-TS CEN ISO/TS 21268-2:2010

TECHNICAL ISO/TS
SPECIFICATION 21268-2
First edition
2007-07-15

Soil quality — Leaching procedures for
subsequent chemical and
ecotoxicological testing of soil and soil
materials
Part 2:
Batch test using a liquid to solid ratio of
10 l/kg dry matter
Qualité du sol — Modes opératoires de lixiviation en vue d'essais
chimiques et écotoxicologiques ultérieurs des sols et matériaux du sol
Partie 2: Essai en bâchée avec un rapport liquide/solide de 10 l/kg de
matière sèche




Reference number
ISO/TS 21268-2:2007(E)
©
ISO 2007

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
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ii © ISO 2007 – All rights reserved

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope .1
2 Normative references .1
3 Terms and definitions .2
4 Principle.3
5 Reagents.3
6 Apparatus .4
7 Sample pre-treatment.5
7.1 Sample size .5
7.2 Particle size reduction.5
7.3 Determination of dry matter content and water content.6
7.4 Preparation of test portion.6
8 Procedure .7
8.1 Testing conditions.7
8.2 Description of the procedure.7
8.2.1 Leaching step.7
8.2.2 Liquid-solid separation step.7
8.3 Further preparation of the eluate for analysis .8
8.4 Blank test for the application of the leaching procedure .8
9 Calculation.9
10 Test report .9
11 Analytical determination .10
11.1 General.10
11.2 Calculations and blank test information .10
12 Performance characteristics .10
Annex A (informative) Information on the influence on the test results of the parameters that
affect leaching.11
Annex B (informative) Example of a specific liquid-solid separation procedure for soil samples
(applying only to the leaching of inorganic constituents).13
Bibliography .15

© ISO 2007 – All rights reserved iii

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
In other circumstances, particularly when there is an urgent market requirement for such documents, a
technical committee may decide to publish other types of normative document:
— an ISO Publicly Available Specification (ISO/PAS) represents an agreement between technical experts in
an ISO working group and is accepted for publication if it is approved by more than 50 % of the members
of the parent committee casting a vote;
— an ISO Technical Specification (ISO/TS) represents an agreement between the members of a technical
committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting
a vote.
An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a
further three years, revised to become an International Standard, or withdrawn. If the ISO/PAS or ISO/TS is
confirmed, it is reviewed again after a further three years, at which time it must either be transformed into an
International Standard or be withdrawn.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO/TS 21268-2 was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 7, Soil
and site assessment.
ISO/TS 21268 consists of the following parts, under the general title Soil quality — Leaching procedures for
subsequent chemical and ecotoxicological testing of soil and soil materials:
⎯ Part 1: Batch test using a liquid to solid ratio of 2 l/kg dry matter
⎯ Part 2: Batch test using a liquid to solid ratio of 10 l/kg dry matter
⎯ Part 3: Up-flow percolation test
⎯ Part 4: Influence of pH on leaching with initial acid/base addition
iv © ISO 2007 – All rights reserved

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
Introduction
In various countries, tests have been developed to characterise and assess the constituents which can be
released from materials. The release of soluble constituents upon contact with water is regarded as a main
mechanism of release, which results in a potential risk to the environment during the use or disposal of
materials. The intent of these tests is to identify the leaching properties of materials. The complexity of the
leaching process makes simplifications necessary.
Not all of the relevant aspects of leaching behaviour can be addressed in one standard.
Tests to characterise the behaviour of materials can generally be divided into three categories (EN 12920;
[9]
EN 12457-2) and are addressed in ISO 18772 . The relationships between these tests are summarised
below.
a) “Basic characterisation” tests are used to obtain information on the short- and long-term leaching
behaviour and characteristic properties of materials. Liquid/solid (L/S) ratios, leachant composition,
factors controlling leachability, such as pH, redox potential, complexing capacity, role of dissolved organic
carbon (DOC), ageing of material and physical parameters, are addressed in these defined tests.
b) “Compliance” tests are used to determine whether the material complies with a specific behaviour or with
specific reference values. These tests focus on key variables and leaching behaviour previously identified
by basic characterisation tests.
c) “On-site verification” tests are used as a rapid check to confirm that the material is the same as that which
has been subjected to the compliance test(s). On-site verification tests are not necessarily leaching tests.
The test procedure described in this method belongs to category b): compliance tests.
NOTE Up to now, the test procedures described in this part of ISO/TS 21268 have not been validated.

© ISO 2007 – All rights reserved v

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SIST-TS CEN ISO/TS 21268-2:2010
TECHNICAL SPECIFICATION ISO/TS 21268-2:2007(E)

Soil quality — Leaching procedures for subsequent chemical
and ecotoxicological testing of soil and soil materials
Part 2:
Batch test using a liquid to solid ratio of 10 l/kg dry matter
1 Scope
This part of ISO/TS 21268 specifies a test providing information on leaching of soil and soil materials under
the experimental conditions specified hereafter, and particularly at a liquid to solid ratio of 10 l/kg dry matter. It
applies to soil and soil material with a particle size less than or equal to 4 mm.
This part of ISO/TS 21268 has been developed to measure the release of inorganic and organic constituents
from soil and soil material and the ecotoxicological effects of eluates with respect to micro-organisms, fauna
and flora. The test is not suitable for constituents that are volatile under ambient conditions. For
ecotoxicological testing, see ISO 15799.
NOTE 1 Volatile organic constituents include the low-molecular-weight components in mixtures such as mineral oil.
NOTE 2 It is not always possible to optimise test conditions simultaneously for inorganic and organic constituents and
optimum test conditions may also vary between different groups of organic constituents. Test requirements for organic
constituents are generally more stringent than those for inorganic constituents. The test conditions suitable for measuring
the release of organic constituents will generally also be applicable to inorganic constituents.
NOTE 3 For ecotoxicological testing, eluates representing the release of both inorganic and organic contaminants are
needed. In this document, ecotoxicological testing is also meant to include genotoxicological testing.
The test procedure specified in this part of ISO/TS 21268 produces eluates, which are subsequently
characterised by existing physical, chemical and ecotoxicological standard methods.
This test is mainly aimed at being used for routine and control purposes, and it cannot be used alone to
describe all leaching properties of a soil. Additional leaching tests are needed for that extended goal. This part
of ISO/TS 21268 does not address issues related to health and safety. It only determines the leaching
properties as outlined in Clause 4.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of samples
ISO 7027, Water quality — Determination of turbidity
ISO 10381-1, Soil quality — Sampling — Part 1: Guidance on the design of sampling programmes
ISO 10381-2, Soil quality — Sampling — Part 2: Guidance on sampling techniques
© ISO 2007 – All rights reserved 1

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
ISO 10381-3, Soil quality — Sampling — Part 3: Guidance on safety
ISO 10381-4, Soil quality — Sampling — Part 4: Guidance on the procedure for investigation of natural,
near-natural and cultivated sites
ISO 10381-5, Soil quality — Sampling — Part 5: Guidance on the procedure for the investigation of urban and
industrial sites with regard to soil contamination
ISO 10381-6, Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil
under aerobic conditions for the assessment of microbialogical processes, biomass and diversity in the
laboratory
ISO 10523, Water quality — Determination of pH
ISO 11465, Soil quality — Determination of dry matter and water content on a mass basis — Gravimetric
method
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
leaching test
test during which a material is put into contact with a leachant under strictly defined conditions and some
constituents of the material are extracted
3.2
leachant
liquid used in a leaching test
NOTE For the purpose of this part of ISO/TS 21268 the leachant is water as specified in 5.1.
3.3
eluate
solution recovered from a leaching test
3.4
liquid to solid ratio
L/S
ratio between the total volume of liquid (L in litres), which in this extraction is in contact with the soil sample,
and the dry mass of the sample (S in kg of dry matter).
NOTE L/S is expressed in l/kg.
3.5
dry matter content
w
dm
ratio, expressed in percent, between the mass of the dry residue, determined in accordance with ISO 11465,
and the corresponding raw mass
3.6
water content
w
H O
2
ratio, expressed in percent, between the mass of water contained in the material as received and the
corresponding dry residue of the material
NOTE The basis for the calculation of the moisture content is the mass of the dry residue in this part of
ISO/TS 21268, as specified in ISO 11465 (for the determination of the water content of soil).
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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
3.7
laboratory sample
sample or sub-sample(s) sent to or received by the laboratory
3.8
test sample
sample, prepared from the laboratory sample, from which test portions are removed for testing or analysis
3.9
test portion
quantity of material of appropriate size for measurement of the concentration or other properties of interest
taken from the test sample
NOTE 1 The test portion can be taken from the laboratory sample directly if no pre-treatment of the sample is required,
but usually it is taken from the test sample.
NOTE 2 A unit or increment of proper homogeneity, size and fineness, needing no further preparation, can be a test
portion.
3.10
soil material
excavated soil, dredged materials, manufactured soils, treated soils and fill materials
[ISO 15176:2002, definition 3.1.4]
4 Principle
The test portion, which originally or after suitable pre-treatment has a particle size less than or equal to 4 mm,
is brought into contact with water containing a low concentration (0,001 M) of calcium chloride under defined
conditions. The standard method is based on the assumption that equilibrium or near-equilibrium is achieved
between the liquid and solid phases during the test period. The solid residue is subsequently separated from
the liquid. The separation procedure may strongly influence the test results and shall be particularly stringent
for organic constituents. The properties of the eluate are measured using methods developed for water
analysis adapted to meet criteria for analysis of eluates, and/or the eluate may be subjected to subsequent
ecotoxicological testing.
After the test, the leaching conditions, in terms of pH, electrical conductivity or DOC and, optionally, redox
potential or turbidity dictated by the material, shall be recorded.
NOTE 1 These parameters often control the leaching behaviour of soil materials and are therefore important for
evaluation of the test results.
NOTE 2 The leachant is 0,001 M CaCl to minimize the mobilisation of DOC caused by an ionic strength of the
2
leachant which is too low.
The procedure described in this part of ISO/TS 21268 is based on the more stringent test requirements for
determining the release of organic constituents and for subsequent ecotoxicological testing. If only the release
of inorganic constituents is to be measured, less stringent requirements may be adapted for some steps of the
procedure.
5 Reagents
5.1 Demineralised water or deionised water or water of equivalent purity (5 < pH < 7,5) with a
conductivity < 0,5 mS/m in accordance with grade 3 specified in ISO 3696, made to 0,001 M CaCl . For
2
eluates that are not to be used for ecotoxicological testing, sodium azide (NaN ) shall be added to a resulting
3
concentration of 0,1 % in order to prevent microbial degradation of organic contaminants.
© ISO 2007 – All rights reserved 3

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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
NOTE 1 Microbial degradation of organic contaminants may occur in eluates without NaN .
3
NOTE 2 If only inorganic compounds are measured, the addition of NaN is not required.
3
5.2 Rinsing solutions: nitric acid 0,1 mol/l (analytical grade) and/or organic solvent (acetone).
6 Apparatus
6.1 Borosilicate glass of high purity in accordance with ISO 5667-3, with a nominal volume of 1 l, glass
bottles having caps of inert material, for example PTFE (polytetrafluoroethylene). Rinsing is compulsory and it
should be assured that previously used bottles have no background level of analytes.
NOTE 1 If only inorganic parameters are analysed, alternative materials, such as HDPE/PP bottles, can be used,
except for unpreserved samples for mercury analysis.
The volume of 1 l is selected in combination with the mass m of 90 g as specified in 7.4 in order to minimise
head-space at a L/S ratio of 10 l/kg dry matter. In the case of materials with low density, deviation from this
requirement can be necessary while still ensuring minimum headspace. This deviation should be reported.
NOTE 2 Glass of high quality is considered adequate for both metals and organic contaminants, particularly, since the
pH range usually covered in soil testing does not reach the conditions (pH > 10 and pH < 3) where glass itself may be
partially dissolved.
NOTE 3 Heat treatment of used glassware at 550 °C can be used to remove traces of analytes. However, this
treatment has been shown to increase adsorption of organic substances from the air.
6.2 Glass bottle with a nominal volume of 5 l, to be used when samples from replicate tests are
recombined after centrifugation for further analysis or testing.
−1 −1 −1
6.3 End-over-end tumbler (5 min to 10 min ) or roller table rotating at about 10 min .
Other shaking devices may be used, provided that they can be shown to provide equivalent results. These
agitation devices are specified because excessive abrasion leading to significant particle size reduction should
be avoided.
6.4 Filtration apparatus, either a vacuum filtration device (between 2,5 kPa and 4,0 kPa) or a
high-pressure filtration apparatus (< 0,5 MPa). Rinsing is compulsory. When semi-volatile components are to
be analysed, vacuum filtration shall not be used.
6.5 0,45 µm membrane filters, pre-rinsed or similarly clean, for filtration [e.g. rinsed with 0,1 mol/l HNO
3
(5.2) and water (5.1)].
The filter should be chosen so as not to adsorb (or release) compounds of interest. This could be tested in
preliminary experiments.
6.6 Sieving equipment with sieves of 4 mm nominal screen size.
NOTE Due to sieving, contamination of the sample can occur to an extent which affects the leaching of some
constituents of concern, e.g. chromium, nickel and molybdenum from stainless steel equipment or plasticisers from plastic
sieves.
6.7 Centrifuge operating at 20 000 g to 30 000 g using centrifuge tubes of fluorinated ethylene propylene
(FEP) or tubes of an alternative material which is inert with regard to both inorganic and organic compounds
and suitable for high-speed centrifugation.
Alternatively, if a high-speed centrifuge is not available, a centrifuge operating at 2 000 g to 2 500 g using
glass bottles may be used in combination with increased centrifugation time. Cooling shall be applied to
maintain the desired temperature.
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SIST-TS CEN ISO/TS 21268-2:2010
ISO/TS 21268-2:2007(E)
6.8 Device for measuring electrical conductivity.
6.9 pH meter in accordance with ISO 10523.
6.10 Two thermometers for air and leachant temperature measurement.
6.11 Redox potential meter (optional).
6.12 Balance with an accuracy of at least 0,1 g.
6.13 Measuring cylinders for volume determination with 1 % accuracy.
6.14 Sample splitter for sub-sampling of laboratory samples (optional).
6.15 Turbidity meter as specified in ISO 7027.
6.16 Crushing equipment: a jaw crusher.
NOTE Due
...