SIST ISO 5985:2003

Animal feeding stuffs -- Determination of ash insoluble in hydrochloric acid

ISO 5985:2002 specifies two procedures for animal feeding stuffs for the determination of the ash which is insoluble in hydrochloric acid.
The applicable procedure depends on the nature of the sample.
Procedure A is applicable to simple organic animal feeding stuffs and to compound feeding stuffs, except those mentioned under procedure B.
Procedure B is applicable to minerals, mineral mixtures and compound feeding stuffs of which the ash insoluble in hydrochloric acid exceeds 1 % (mass fraction), as determined by procedure A.

Aliments des animaux -- Détermination des cendres insolubles dans l'acide chlorhydrique

L'ISO 5985:2002 sp�cifie deux modes op�ratoires applicables aux aliments des animaux permettant de doser les cendres insolubles dans l'acide chlorhydrique.
Le mode op�ratoire � appliquer est fonction de la nature de l'�chantillon.
Le mode op�ratoire A est applicable aux aliments des animaux simples et aux aliments compos�s non min�raux (� l'exception de ceux pour lesquels le mode op�ratoire B s'applique).
Le mode op�ratoire B est applicable aux min�raux, aux m�langes min�raux et aux aliments compos�s dont la teneur en cendres insolubles dans l'acide chlorhydrique est sup�rieure � 1 % (fraction massique), lorsqu'elle est d�termin�e selon le mode op�ratoire A.

Krma - Določevanje pepela, netopnega v klorovodikovi kislini

General Information

• Status: Published
• Publication Date: 30-Apr-2003
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 01-May-2003
• Due Date: 01-May-2003
• Completion Date: 01-May-2003
• Ref Project: ISO 5985:2002 - Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid

Relations

• Revised: SIST ISO 5985:1995 - Animal feeding stuffs -- Determination of ash insoluble in hydrochloric acid
• Effective Date: 12-May-2008
• Revised: ISO 5985:1978 - Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid
• Effective Date: 12-May-2008

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 5985
Second edition
2002-11-15
Animal feeding stuffs — Determination
of ash insoluble in hydrochloric acid
Aliments des animaux — Détermination des cendres insolubles dans
l'acide chlorhydrique





Reference number
ISO 5985:2002(E)
©
ISO 2002

---------------------- Page: 1 ----------------------
ISO 5985:2002(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not
be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this
file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this
area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event
that a problem relating to it is found, please inform the Central Secretariat at the address given below.


©  ISO 2002
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body
in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.ch
Printed in Switzerland

ii © ISO 2002 — All rights reserved

---------------------- Page: 2 ----------------------
ISO 5985:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted
by the technical committees are circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identiying any or all such patent rights.
ISO 5985 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding
stuffs.
This second edition cancels and replaces the first edition (ISO 5985:1978), of which it constitutes a minor revision.
Annex A of this International Standard is for information only.

© ISO 2002 — All rights reserved iii

---------------------- Page: 3 ----------------------
INTERNATIONAL STANDARD ISO 5985:2002(E)

Animal feeding stuffs — Determination of ash insoluble
in hydrochloric acid
1 Scope
This International Standard specifies two procedures for animal feeding stuffs for the determination of the ash
which is insoluble in hydrochloric acid.
The applicable procedure depends on the nature of the sample.
a) Procedure A is applicable to simple organic animal feeding stuffs and to compound feeding stuffs (except
those mentioned under procedure B).
b) Procedure B is applicable to minerals, mineral mixtures and compound feeding stuffs of which the ash
insoluble in hydrochloric acid exceeds 1 % (mass fraction), as determined by procedure A.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 6498, Animal feeding stuffs — Preparation of test samples
3 Term and definition
For the purposes of this International Standard, the following term and definition applies.
3.1
ash insoluble in hydrochloric acid
that part of the ash which is insoluble in dilute hydrochloric acid under the conditions specified in this International
Standard
NOTE It is expressed as a mass fraction of the sample in percent.
4 Principle
4.1 Procedure A
The organic matter in a test portion is decomposed by incineration.
The ash obtained is treated with hydrochloric acid. The mixture is filtered, followed by drying, incineration and
weighing of the residue.
© ISO 2002 — All rights reserved 1

---------------------- Page: 4 ----------------------
ISO 5985:2002(E)
4.2 Procedure B
A test portion is treated with hydrochloric acid. The mixture is filtered, followed by drying and incineration.
The ash is treated as in 4.1.
5 Reagents
Use only reagents of analytical quality, and distilled or demineralized water or water of at least equivalent purity.
5.1 Dilute hydrochloric acid, 3 mol/l.
5.2 Trichloroacetic acid solution, 200 g/l.
5.3 Trichloroacetic acid solution, 10 g/l.
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1 Analytical balance, capable of weighing to the nearest 0,001 g.
6.2 Muffle furnace, electrically heated, thermostatically controlled, and provided with a pyrometer.
The furnace, when set at 550 °C, shall be capable of being controlled in such a way that the temperature in the
places where the incineration dishes will be placed will not differ by more than 20 °C from this set temperature.
6.3 Drying oven, capable of being controlled at (103 ± 2) °C.
6.4 Hot-plate or gas burner
6.5 Boiling water bath
6.6 Incineration dishes, of platinum or platinum-gold alloy (e.g. 10 % Pt, 90 % Au) or of other material
2
unaffected by the conditions of the test, preferably rectangular, with a surface area of about 20 cm and a height of
about 2,5 cm.
2
For samples which are inclined to swell on carbonization, use dishes with a surface area of about 30 cm and a
height of about 3 cm.
6.7 Desiccator, provided with an effective desiccant.
7 Sampling
It is important that the laboratory receive a sample which is truly representative and has not been damaged or
changed during transport or storage.
Store the sample in such a way that deterioration and change in composition are prevented.
Sampling is not part of the method specified in this International Standard. A recommended sampling method is
given in ISO 6497.
2 © ISO 2002 — All rights reserved

---------------------- Page: 5 ----------------------
ISO 5985:2002(E)
8 Procedures
8.1 Preparation of test sample
Prepare the test sample in acordance with ISO 6498.
8.2 Procedure A
8.2.1 Test portion
Weigh, to the nearest 0,001 g, about 5 g of the test sample (8.1) into an incineration dish (6.6).
8.2.2 Determination
8.2.2.1 Place the incineration dish containing the test porti
...

SLOVENSKI STANDARD
SIST ISO 5985:2003
01-maj-2003
.UPD'RORþHYDQMHSHSHODQHWRSQHJDYNORURYRGLNRYLNLVOLQL
Animal feeding stuffs -- Determination of ash insoluble in hydrochloric acid
Aliments des animaux -- Détermination des cendres insolubles dans l'acide
chlorhydrique
Ta slovenski standard je istoveten z: ISO 5985:2002
ICS:
65.120 Krmila Animal feeding stuffs
SIST ISO 5985:2003 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST ISO 5985:2003

---------------------- Page: 2 ----------------------

SIST ISO 5985:2003

INTERNATIONAL ISO
STANDARD 5985
Second edition
2002-11-15
Animal feeding stuffs — Determination
of ash insoluble in hydrochloric acid
Aliments des animaux — Détermination des cendres insolubles dans
l'acide chlorhydrique





Reference number
ISO 5985:2002(E)
©
ISO 2002

---------------------- Page: 3 ----------------------

SIST ISO 5985:2003
ISO 5985:2002(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not
be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this
file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat accepts no liability in this
area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters
were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In the unlikely event
that a problem relating to it is found, please inform the Central Secretariat at the address given below.


©  ISO 2002
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO's member body
in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.ch
Printed in Switzerland

ii © ISO 2002 — All rights reserved

---------------------- Page: 4 ----------------------

SIST ISO 5985:2003
ISO 5985:2002(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
The main task of technical committees is to prepare International Standards. Draft International Standards adopted
by the technical committees are circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identiying any or all such patent rights.
ISO 5985 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding
stuffs.
This second edition cancels and replaces the first edition (ISO 5985:1978), of which it constitutes a minor revision.
Annex A of this International Standard is for information only.

© ISO 2002 — All rights reserved iii

---------------------- Page: 5 ----------------------

SIST ISO 5985:2003

---------------------- Page: 6 ----------------------

SIST ISO 5985:2003
INTERNATIONAL STANDARD ISO 5985:2002(E)

Animal feeding stuffs — Determination of ash insoluble
in hydrochloric acid
1 Scope
This International Standard specifies two procedures for animal feeding stuffs for the determination of the ash
which is insoluble in hydrochloric acid.
The applicable procedure depends on the nature of the sample.
a) Procedure A is applicable to simple organic animal feeding stuffs and to compound feeding stuffs (except
those mentioned under procedure B).
b) Procedure B is applicable to minerals, mineral mixtures and compound feeding stuffs of which the ash
insoluble in hydrochloric acid exceeds 1 % (mass fraction), as determined by procedure A.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 6498, Animal feeding stuffs — Preparation of test samples
3 Term and definition
For the purposes of this International Standard, the following term and definition applies.
3.1
ash insoluble in hydrochloric acid
that part of the ash which is insoluble in dilute hydrochloric acid under the conditions specified in this International
Standard
NOTE It is expressed as a mass fraction of the sample in percent.
4 Principle
4.1 Procedure A
The organic matter in a test portion is decomposed by incineration.
The ash obtained is treated with hydrochloric acid. The mixture is filtered, followed by drying, incineration and
weighing of the residue.
© ISO 2002 — All rights reserved 1

---------------------- Page: 7 ----------------------

SIST ISO 5985:2003
ISO 5985:2002(E)
4.2 Procedure B
A test portion is treated with hydrochloric acid. The mixture is filtered, followed by drying and incineration.
The ash is treated as in 4.1.
5 Reagents
Use only reagents of analytical quality, and distilled or demineralized water or water of at least equivalent purity.
5.1 Dilute hydrochloric acid, 3 mol/l.
5.2 Trichloroacetic acid solution, 200 g/l.
5.3 Trichloroacetic acid solution, 10 g/l.
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1 Analytical balance, capable of weighing to the nearest 0,001 g.
6.2 Muffle furnace, electrically heated, thermostatically controlled, and provided with a pyrometer.
The furnace, when set at 550 °C, shall be capable of being controlled in such a way that the temperature in the
places where the incineration dishes will be placed will not differ by more than 20 °C from this set temperature.
6.3 Drying oven, capable of being controlled at (103 ± 2) °C.
6.4 Hot-plate or gas burner
6.5 Boiling water bath
6.6 Incineration dishes, of platinum or platinum-gold alloy (e.g. 10 % Pt, 90 % Au) or of other material
2
unaffected by the conditions of the test, preferably rectangular, with a surface area of about 20 cm and a height of
about 2,5 cm.
2
For samples which are inclined to swell on carbonization, use dishes with a surface area of about 30 cm and a
height of about 3 cm.
6.7 Desiccator, provided with an effective desiccant.
7 Sampling
It is important that the laboratory receive a sample which is truly representative and has not been damaged or
changed during transport or storage.
Store the sample in such a way that deterioration and change in composition are prevented.
Sampling is not part of the method specified in this International Standard. A recommended sampling method is
given in ISO 6497.
...

NORME ISO
INTERNATIONALE 5985
Deuxième édition
2002-11-15
Aliments des animaux — Dosage
des cendres insolubles dans l’acide
chlorhydrique
Animal feeding stuffs — Determination of ash insoluble in
hydrochloric acid
Numéro de référence
ISO 5985:2002(F)
©
ISO 2002

---------------------- Page: 1 ----------------------
ISO 5985:2002(F)

DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2002
Droits de reproduction réservés. Sauf indication contraire, aucune partie de cette publication ne peut être reproduite ni utilisée
sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique, y compris la photocopie, l’affichage sur
l’internet ou sur un Intranet, sans autorisation écrite préalable. Les demandes d’autorisation peuvent être adressées à l’ISO à
l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Version française parue en 2013
Publié en Suisse
ii © ISO 2002 – Tous droits réservés

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ISO 5985:2002(F)

Sommaire Page
Avant-propos .iv
1 Domaine d’application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe . 1
4.1 Mode opératoire A . 1
4.2 Mode opératoire B . 1
5 Réactifs . 2
6 Appareillage . 2
7 Échantillonnage . 2
8 Modes opératoires . 3
8.1 Préparation de l’échantillon pour essai . 3
8.2 Mode opératoire A . 3
8.3 Mode opératoire B . 3
9 Expression des résultats. 4
10 Fidélité . 4
10.1 Essais interlaboratoires . . 4
10.2 Répétabilité . 4
10.3 Reproductibilité . 5
11 Rapport d’essai . 5
Annexe A (informative) Résultats des essais interlaboratoires . 6
Bibliographie . 7
© ISO 2002 – Tous droits réservés iii

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ISO 5985:2002(F)

Avant-propos
L’ISO (Organisation internationale de normalisation) est une fédération mondiale d’organismes
nationaux de normalisation (comités membres de l’ISO). L’élaboration des Normes internationales est
en général confiée aux comités techniques de l’ISO. Chaque comité membre intéressé par une étude
a le droit de faire partie du comité technique créé à cet effet. Les organisations internationales,
gouvernementales et non gouvernementales, en liaison avec l’ISO participent également aux travaux.
L’ISO collabore étroitement avec la Commission électrotechnique internationale (CEI) en ce qui concerne
la normalisation électrotechnique.
Les Normes internationales sont rédigées conformément aux règles données dans les Directives
ISO/CEI, Partie 3.
La tâche principale des comités techniques est d’élaborer les Normes internationales. Les projets de
Normes internationales adoptés par les comités techniques sont soumis aux comités membres pour vote.
Leur publication comme Normes internationales requiert l’approbation de 75 % au moins des comités
membres votants.
L’attention est appelée sur le fait que certains des éléments du présent document peuvent faire l’objet de
droits de propriété intellectuelle ou de droits analogues. L’ISO ne saurait être tenue pour responsable de
ne pas avoir identifié de tels droits de propriété et averti de leur existence.
L’ISO 5985 a été élaborée par le comité technique ISO/TC 34, Produits alimentaires, sous-comité SC 10,
Aliments des animaux.
Cette deuxième édition annule et remplace la première édition (ISO 5985:1978), dont elle constitue une
révision mineure.
L’Annexe A de la présente Norme internationale est donnée à titre d’information uniquement.
La présente version française de l’ISO 5985:2002 inclut le Rectificatif technique ISO 5985:2002/Cor.1:2005
à la version anglaise publié le 2005-03-01.
iv © ISO 2002 – Tous droits réservés

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NORME INTERNATIONALE ISO 5985:2002(F)
Aliments des animaux — Dosage des cendres insolubles
dans l’acide chlorhydrique
1 Domaine d’application
La présente Norme internationale spécifie deux modes opératoires applicables aux aliments des animaux
permettant de doser les cendres insolubles dans l’acide chlorhydrique.
Le mode opératoire à appliquer est fonction de la nature de l’échantillon.
a) Le mode opératoire A est applicable aux aliments des animaux simples et aux aliments composés
non minéraux (à l’exception de ceux pour lesquels le mode opératoire B s’applique).
b) Le mode opératoire B est applicable aux minéraux, aux mélanges minéraux et aux aliments composés
dont la teneur en cendres insolubles dans l’acide chlorhydrique est supérieure à 1 % (fraction
massique), lorsqu’elle est déterminée selon le mode opératoire A.
2 Références normatives
Les documents normatifs suivants contiennent des dispositions qui par suite de la référence qui y est
faite, constituent des dispositions valables pour la présente Norme internationale. Pour les références
datées, les amendements ultérieurs ou les révisions de ces publications ne s’appliquent pas. Toutefois, les
parties prenantes aux accords fondés sur la présente Norme internationale sont invitées à rechercher
la possibilité d’appliquer l’édition la plus récente du document normatif indiqué ci-après. Pour les
références non datées, la dernière édition du document normatif en référence s’applique. Les membres
de l’ISO et de la CEI possèdent le registre des normes internationales en vigueur à un moment donné.
ISO 6498, Aliments des animaux — Préparation des échantillons pour essai
3 Termes et définitions
Pour les besoins de la présente Norme internationale, le terme et la définition suivants s’appliquent.
3.1
cendres insolubles dans l’acide chlorhydrique
cendres qui ne peuvent pas être dissoutes dans de l’acide chlorhydrique dilué dans les conditions
spécifiées par la présente Norme internationale
Note 1 à l’article: La teneur en cendres insolubles dans l’acide chlorhydrique est exprimée en fraction massique de
l’échantillon, en pourcentage.
4 Principe
4.1 Mode opératoire A
La matière organique contenue dans une prise d’essai est décomposée par incinération.
Les cendres ainsi obtenues sont traitées avec de l’acide chlorhydrique. Le mélange est ensuite filtré,
séché, incinéré, puis le résidu est pesé.
4.2 Mode opératoire B
Une prise d’essai est traitée avec de l’acide chlorhydrique. Le mélange est filtré, séché, puis incinéré.
© ISO 2002 – Tous droits réservés 1

---------------------- Page: 5 ----------------------
ISO 5985:2002(F)

Les cendres sont ensuite traitées selon 4.1.
5 Réactifs
Utiliser uniquement des réactifs de qualité analytique et de l’eau distillée, déminéralisée ou de pureté
au moins équivalente.
5.1 Acide chlorhydrique dilué, 3 mol/l.
5.2 Solution d’acide trichloroacétique, 200 g/l.
5.3 Solution d’acide trichloroacétique, 10 g/l.
6 Appareillage
Matériel courant de laboratoire et, en particulier, ce qui suit.
6.1 Balance analytique, pouvant peser à 0,001 g près.
6.2 Four à moufle, chauffé électriquement, à commande thermostatique et muni d’un pyromètre.
Une fois réglé à 550 °C, le four doit pouvoir être régulé de sorte que la température ne varie pas de plus
de 20 °C, par rapport à la température fixé
...