SIST-TS CEN/TS 839:2008
(Main)Wood preservatives - Determination of the protective effectiveness against wood destroying basidiomycetes - Application by surface treatment
Wood preservatives - Determination of the protective effectiveness against wood destroying basidiomycetes - Application by surface treatment
This European Standard specifies a test method for determining the protective effectiveness of a wood preservative applied to the surface of the wood, against wood destroying basidiomycetes cultured on an agar medium.
The method is applicable to all products that are to be applied by superficial application processes. This includes:
organic solvent-based wood preservatives; or
organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates; or
water-soluble products; or
chemicals that are being studied as active ingredients for application by superficial processes.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
Holzschutzmittel - Bestimmung der vorbeugenden Wirksamkeit gegen holzzerstörende Basidiomyceten - Anwendung mit Oberflächenverfahren
6
Diese Europäische Norm legt ein Prüfverfahren zur Bestimmung der vorbeugenden Wirksamkeit eines auf die
Holzoberfläche aufgebrachten Holzschutzmittels gegen auf einem Agarmedium gezüchtete holzzerstörende
Basidiomyceten fest.
Das Verfahren ist auf alle Produkte anwendbar, die für die Anwendung mit Oberflächenverfahren bestimmt
sind. Dazu gehören:
- Holzschutzmittel auf der Grundlage organischer Lösemittel;
- wasserdispergierbare organische Formulierungen im Anlieferungszustand oder in der Form, in der sie im
Laboratorium durch Verdünnung von Konzentraten hergestellt werden;
- wasserlösliche Produkte;
- Chemikalien, die als Wirkstoffe für die Anwendung mit Oberflächenverfahren untersucht werden.
ANMERKUNG Dieses Verfahren kann in Verbindung mit einer Alterungsbeanspruchung, z. B nach EN 73, angewendet
werden.
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-a-vis des champignons basidiomycetes lignivores - Application par traitement de surface
La présente Norme européenne spécifie une méthode d’essai de la détermination de l’efficacité protectrice d’un produit de préservation du bois, appliqué par un traitement de surface, vis a vis des champignons basidiomycetes lignivores cultivés sur milieu gélosé.
Cette méthode est applicable a tous les produits qui sont appliqués par des procédés par application superficielle. Cela comprend :
les produits de préservation du bois solubles dans les solvants organiques ;
des formulations organiques hydrodispersables telles qu’elles sont livrées ou préparées en laboratoire a partir de concentrés ; ou
des produits hydrosolubles ; ou
des produits chimiques étudiés en tant que matieres actives pour l’application par des procédés de surface.
NOTE Cette méthode peut etre utilisée conjointement a une épreuve de vieillissement, par exemple celle décrite dans l’EN 73.
Zaščitna sredstva za les - Ugotavljanje preventivne učinkovitosti zaščitnih sredstev proti glivam odprtotrosnicam - Površinsko nanašanje
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST-TS CEN/TS 839:2008
01-julij-2008
1DGRPHãþD
SIST-TS ENV 839:2004
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHSUHYHQWLYQHXþLQNRYLWRVWL]DãþLWQLK
VUHGVWHYSURWLJOLYDPRGSUWRWURVQLFDP3RYUãLQVNRQDQDãDQMH
Wood preservatives - Determination of the protective effectiveness against wood
destroying basidiomycetes - Application by surface treatment
Holzschutzmittel - Bestimmung der vorbeugenden Wirksamkeit gegen holzzerstörende
Basidiomyceten - Anwendung mit Oberflächenverfahren
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-a-vis des
champignons basidiomycetes lignivores - Application par traitement de surface
Ta slovenski standard je istoveten z: CEN/TS 839:2008
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
SIST-TS CEN/TS 839:2008 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
TECHNICAL SPECIFICATION
CEN/TS 839
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
March 2008
ICS 71.100.50 Supersedes ENV 839:2002
English Version
Wood preservatives - Determination of the protective
effectiveness against wood destroying basidiomycetes -
Application by surface treatment
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis-à-vis des champignons Wirksamkeit gegen holzzerstörende Basidiomyceten -
basidiomycètes lignivores - Application par traitement de Anwendung mit Oberflächenverfahren
surface
This Technical Specification (CEN/TS) was approved by CEN on 5 November 2007 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 839:2008: E
worldwide for CEN national Members.
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CEN/TS 839:2008 (E)
Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative references .5
3 Terms and definitions .5
4 Principle.6
5 Test materials and apparatus.6
5.1 Biological material.6
5.2 Products and reagents.7
5.3 Apparatus .8
6 Sampling of the preservative.8
7 Test specimens.8
7.1 Species of wood.8
7.2 Wood quality .9
7.3 Provision of the test specimens.9
7.4 Dimensions and density of test specimens.9
7.5 Number and distribution of test specimens .9
8 Procedure .10
8.1 Preparation of the untreated test specimens .10
8.2 Preparation of the treated test specimens.10
8.3 Exposure to fungi .12
8.4 Culture conditions and duration of test .12
8.5 Assessment of test.12
9 Statement of results .13
10 Test report .13
Annex A (informative) Test fungi .15
A.1 General information on maintenance and acquisition of test strains.15
A.2 Maintenance and treatment of test fungi .15
A.3 Information regarding obligatory fungi .16
Annex B (normative) Methods of sterilisation .18
B.1 Ionising radiation.18
B.2 Epoxyethane-based sterilant.18
B.3 Epoxypropane-based sterilant .18
B.4 Steam .19
Annex C (informative) Examination of colonisation .20
Annex D (informative) Example of a test report .23
Annex E (informative) Environmental, health and safety precautions within chemical/biological
laboratory .26
Bibliography .27
2
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CEN/TS 839:2008 (E)
Foreword
This document (CEN/TS 839:2008) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes ENV 839:2002.
Significant technical differences between this standard and ENV 839:2002 are as follows:
a) change of assessment from, visual examination for decay supplemented with culturing to assess
colonisation of the interior, to determination of loss in mass due to fungal decay as well as visual
examination for decay of the surface and the interior of the test specimens. The visual examination is now
included as an optional means of assessment to determine colonisation and that this requires an
additional series of test specimens (Annex C).
b) addition in 7.5 of treated check test specimens for calculation of the correction value;
c) taking into account of the terms given in EN 1001-1and the definitions of EN 1001-2;
d) Introduction of an informative Annex E to take account of consideration for minimisation of environmental
and health hazards caused by the use of this biological test.
This standard includes Annexes A, C, D and E that are informative and an Annex B that is normative.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
3
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CEN/TS 839:2008 (E)
Introduction
This European Standard specifies a laboratory method of test which gives a basis for assessing the
effectiveness of a wood preservative, when applied as a surface treatment, against wood destroying
basidiomycetes. It tests whether the applied treatment is able to prevent the penetration of the fungi into the
untreated interior of the test specimens under the conditions of test.
This laboratory method provides one criterion by which the effectiveness of a product can be assessed. In
making this assessment, the methods by which the preservative may be applied should be taken into account.
It is also recommended that results from this test should be supplemented by those from other relevant tests
and above all by practical experience.
The procedures described in this standard method are intended to be carried out by suitably trained and/or
supervised specialists.
Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities,
conditioning chambers and special training for personnel. Also see Annex E for environmental, health and
safety precautions.
4
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CEN/TS 839:2008 (E)
1 Scope
This European Standard specifies a method of test for the determination of the protective effectiveness of a
wood preservative, applied to the surface of the wood, against wood destroying basidiomycetes cultured on
an agar medium.
The method is applicable to all products which are to be applied by superficial application processes. This
includes :
organic solvent-based wood preservatives ; or
organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of
concentrates ; or
water-soluble products ; or
chemicals which are being studied as active ingredients for application by superficial processes.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987).
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply:
3.1
representative sample
sample having its physical and/or chemical characteristics identical to the volumetric average characteristics
of the total volume being sampled.
[EN 1001-2,4.71]
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested).
[Adapted from EN 1001-2,4.83]
3.3
superficial application process
process which does not include particular features or procedures intended to overcome the natural resistance
of wood to penetration of a wood preservative in its ready to use form
[EN 1001-2,4.82]
5
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CEN/TS 839:2008 (E)
4 Principle
Several series of test specimens of a susceptible wood species are end-sealed with a material to prevent
penetration of the wood preservative under test into the end grain of the test specimens. The end-sealed test
specimens are treated with the wood preservative under test using the process and application rate specified
by the supplier.
NOTE Suitable application methods are brushing, pipetting and dipping.
The treated test specimens are exposed to attack by basidiomycetes in pure culture. The performance of the
test product is assessed in terms of its ability to prevent decay as determined by the maximum acceptable
loss in mass and the absence of visible decay of the surface and the untreated interior.
5 Test materials and apparatus
5.1 Biological material
The test fungi to be used are as follows:
5.1.1 Obligatory fungus in all cases
Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %.
5.1.2 Obligatory fungus for particular hazards
Coriolus versicolor (Linnaeus) Quélet (CTB 863A) on hardwood and/or on softwood as appropriate.
Loss in mass of beech in 16 weeks: a mass fraction of minimum 20 %.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 15 %.
5.1.3 Two species to be used compulsorily on the basis of the nature of the test product
For all products except creosote-type products:
Poria placenta (Fries) Cooke sensu J. Eriksson (FPRL 280) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %;
Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %.
For creosotes and similar products:
Lentinus lepideus Fries ex Fries (BAM Ebw. 20) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %;
Lentinus cyathiformis (Schaeffer ex Fries) Bresadola (CTB 67-02B) on hardwood.
Loss in mass of beech in 16 weeks: a mass fraction of minimum 20 %.
5.1.4 Optional fungi
For specific regional uses or conditions, it is also possible to select other fungi on an optional basis.
NOTE When optional fungi are used, information similar to that given in annex A for the obligatory fungi should be
included in the test report.
6
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CEN/TS 839:2008 (E)
5.1.5 Maintenance of strains
The strains shall be maintained and treated (frequency of subculturing, alternation of culture media, etc.) in
accordance with the instructions of their laboratory of origin (see A.2). The parent strain shall be maintained in
the laboratory of its origin so as to conserve and to assure its vigour.
If tests are not undertaken regularly or if a strain shows signs of degeneration a new standard culture of the
strain should be obtained from the laboratory of its origin for each test (see A.2). When new strains are
received, the virulence shall be tested to ensure the strain can achieve the minimum loss in mass (see 5.1.1,
5.1.2 and 5.1.3).
5.2 Products and reagents
5.2.1 Culture medium
The culture medium is a malt agar medium with the following composition:
malt extract :
in concentrated form: (50 ± 0,5) g ;
in powder form: (40 ± 0,5) g ;
agar causing no inhibition of growth of fungi:
(20 ± 0,5) g to (30 ± 0,5) g;
water conforming to grade 3 of EN ISO 3696.
quantity to make up to 1000 ml.
Prepare this medium by warming the mixture in a boiling water bath or steam bath, stirring until completely
dissolved.
Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of 3 mm to
4 mm when in its in-use position. Close the vessels as specified in 5.3.1 and sterilise in an autoclave at
121 °C for 20 min. Let the vessels cool in their in-use position.
5.2.2 Solvents and diluents
For water soluble or water dispersible preservatives:
water conforming to grade 3 of EN ISO 3696.
For preservatives to be diluted or dissolved in an organic solvent:
suitably volatile liquids that leave no residue in the wood that would have a toxic effect on the fungi at the
end of the post-treatment conditioning period.
NOTE Toluene and xylene of recognized analytical grade have been found suitable.
5.2.3 Fumigant (if necessary)
Xylene technical grade.
5.2.4 End-seal compound
A material resistant to the penetration of the wood preservative under test and the test fungi, or separate
materials for each, and without any fungistatic or fungicidal activity within the test specimen.
7
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CEN/TS 839:2008 (E)
NOTE Three brush coats of a 2-component epoxy lacquer, with drying between each application, have been found to
be suitable.
5.3 Apparatus
5.3.1 Culture vessels, Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml,
2 2
providing a flat surface area of between 85 cm and 120 cm for the medium.
NOTE 1 Examples of suitable vessels are given in EN 113.
NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with leak
proof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad of cotton
wool.
5.3.2 Drying oven, capable of being controlled at (103 ± 2) °C.
5.3.3 Desiccators, with efficient desiccant (silica gel for example).
5.3.4 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.5 Drying supports, which will give a minimum contact with the treated test specimens. The supports
shall be of a material that does not react with the test solvent or test wood preservative, for example glass for
organic products.
5.3.6 Culture chamber, (incubator or room), dark and controlled at (22 ± 2) °C and (70 ± 5) % relative
humidity.
5.3.7 Test specimen supports, made of glass, stainless steel or any other inert material, that is to say, with
no risk of having any effect on the culture medium, the fungus, the wood or the test wood preservative, or of
being itself modified. Supports can be capable of holding either one or two test specimens. The supports are
used to prevent direct contact of the test specimens with the culture medium, but shall not separate them from
it by more than 3 mm.
NOTE If abnormally high moisture contents in the test specimens are experienced consistently, use of test specimen
supports of approximately 5 mm thick can help to control the problem. If thicker test specimen supports are used, this
should be recorded in the test report.
5.3.8 Ordinary laboratory equipment, including a balance capable of weighing to the nearest of 0,01 g and
an autoclave.
6 Sampling of the preservative
The sample of the wood preservative shall be representative of the product to be tested. Samples shall be
stored and handled in accordance with any written instructions from the supplier.
NOTE For the sampling of wood preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The species of wood to be used shall be susceptible to attack by fungi and shall be readily penetrated by
liquids.
The reference species are Scots pine (Pinus sylvestris Linnaeus) representing softwoods and beech (Fagus
sylvatica Linnaeus) representing hardwoods.
8
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CEN/TS 839:2008 (E)
Additional tests may be undertaken using other species corresponding to the above characteristics, and of
particular importance for certain countries, but if so this shall be stated in the test report.
7.2 Wood quality
The wood shall be free from cracks, stain, decay, insect damage or other defects. The wood shall not have
been water-stored, floated, chemically treated or steamed.
NOTE Wood that has been kiln dried at temperatures below 60 °C can be used.
The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual
growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole.
The beech shall be even-grained, free from tyloses and discoloration. It shall have between 2 and 6 annual
growth rings per 10 mm.
7.3 Provision of the test specimens
Prepare planed strips having a cross section of (25 ± 0,5) mm × (15 ± 0,5) mm. The longitudinal faces shall be
parallel to the direction of the grain. The annual rings shall have a contact angle of (45 ± 15)° to the broad
faces. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to
give test specimens (50 ± 0,5) mm long.
For treatment, drying and ageing, the test specimens can be retained in planed strips of a length sufficient to
provide one test specimen for exposure to each of the test fungi. Each strip should be end-sealed prior to
treatment.
The specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens and originating from at least five planks.
7.4 Dimensions and density of test specimens
The dimensions of each test specimen at a mass fraction of (12 ± 2) % moisture content shall be
(50 ± 0,5) mm x (25 ± 0,5) mm x (15 ± 0,5) mm.
NOTE A moisture meter of the two-pronged electrical conductivity type is suitable for assessing moisture content.
2
The total surface area of the faces to be treated is theoretically 40 cm but an allowance shall be made for any
encroachment of the sealing compound on to these faces.
In a batch of test specimens to be treated, the density of an individual is permitted to differ from the mean
value of the batch by ± 10 %. This tolerance is increased to ± 20 % for the untreated test specimens. The
mean density for the treated test specimens used for the test shall be recorded in the test report.
7.5 Number and distribution of test specimens
The test specimens are divided into:
e treated test specimens :
1
these are the treated test specimens subjected to attack by the wood destroying fungi. Use at least
six test specimens for each combination of preservative, quantity to be applied, preservative
concentration, test fungus and for each timber species.
In case of dipping select at least 6 test specimens within a range of 10 % from the target retention.
Supplementary samples shall be treated, in order to have a sufficient number of correctly treated
specimens to put in test.
NOTE The treated test specimens are assessed by visual examination for decay of their surfaces and/or interior by the
test fungi. If optional tests for colonisation of the test fungi are required as an additional method of assessment, this should
be carried out on a parallel series of treated test specimens (Annex C).
9
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CEN/TS 839:2008 (E)
e untreated test specimens :
2
e untreated control test specimens : these are untreated test specimens, equal in number to the
2.1
treated test specimens e and of the same wood species, which are placed one in each culture
1
vessel together with a treated test specimen ;
e virulence control test specimens: these are untreated test specimens which are subjected to
2.2
attack by the test fungi to monitor vigour. Use six of these for each combination of test fungus and
timber species used in the test.
— e treated check test specimens for calculation of the correction value:
3
these are test specimens treated in exactly the same way as the e test specimens. Use at least six
1
test specimens for each combination of preservative, quantity to be applied and preservative
concentration and of the same wood species. They are placed, after drying, conditioning and any
appropriate ageing in uninoculated culture vessels, two in each vessel. Variations in mass of these
test specimens make it possible to determine the correction factor (C) of the variations in mass of the
treated test specimens e resulting from factors other than attack by the test fungi. At a given treating
1
concentration, factor C is the mass fraction change of the e test specimens.
3
Mark each specimen so that it can be identified throughout the test.
8 Procedure
8.1 Preparation of the untreated test specimens
Place the numbered untreated test specimens (e and e ) in the oven (5.3.2) and leave them there for 18 h
2.1 2.2
1)
to 24 h . Cool to room temperature in a desiccator (5.3.3) and weigh to the nearest 0,01 g to determine the
initial dry mass (m ). Place the test specimens in the conditioning chamber (5.3.4) until they need to be
0
sterilised (8.3).
NOTE Untreated test specimens are not end-sealed.
8.2 Preparation of the treated test specimens
8.2.1 Preparation
Place the numbered test specimens to be treated (e and e ) in the oven at (103 ± 2) °C (5.3.2) and leave
1 3
1)
them there for 18 h to 24 h . Cool to room temperature in a desiccator (5.3.3) and weigh to the nearest 0,01 g
to determine the initial dry mass (m ). Place the test specimens in the conditioning chamber (5.3.4) until they
0
need to be end-sealed (8.2.2).
1
)
In the case of supplementary tests (7.1) using species of wood other than Scots pine sapwood or beech, this drying
time may need to be longer than 18 h to 24 h; the drying time should be such that the test specimens achieve constant
mass. This can be established by selecting at random from the batch being dried 10 test specimens; after drying and
cooling as directed, determine the total mass, return the test specimens to the oven and repeat the operation at intervals
of not less than 4 h. Constant mass is achieved when the total mass of the selected specimens does not lose more than
0,05 g between weighing.
10
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CEN/TS 839:2008 (E)
8.2.2 End-sealing
Apply the end-sealing compound resistant to the penetration of the test wood preservative (5.2.4) to both end-
grain surfaces of each test specimen to be treated (e and e ). Allow to dry in the conditioning chamber (5.3.4)
1 3
for at least 24 h after the last application.
NOTE Since variation in end seal amount can create errors on mass loss careful operation in applying the end seal
as evenly as possible is required. As quality control guideline a variation within a 10 % limit should be envisaged and
additional absolute mass correction to all specimens could be useful.
8.2.3 Treatment with the test wood preservative
Treat the test specimens e and e on the unsealed longitudinal faces. If application is by brushing or by
1 3
pipette, calculate the amount of test product required to treat each face. Apply the amount evenly to each face
individually and weigh the test specimen before (m ) and after (m ) each application to the nearest 0,01 g.
1 2
Allow to dry between applications. Calculate the uptake of wood preservative solution for each face of each
test specimen (m - m ). Calculate the total uptake for each test specimen and express it in grams of wood
2 1
preservative per square metre of treated surface.
NOTE If the balance has a tare facility, it is easier to tare the balance with the test specimen on it, apply the test
wood preservative, and then record the uptake directly.
If application is by dipping, weigh each test specimen to the nearest 0,01 g (m ), dip for the required time then
1
remove any excess liquid with absorbent paper. Reweigh each test specimen immediately and record the
mass after treatment (m ).
2
Calculate the uptake of wood preservative solution for each test specimen (m - m ) and express it in grams of
2 1
wood preservative per square metre of treated surface.
8.2.4 Drying
Following treatment (8.2.3), place th
...
SLOVENSKI STANDARD
oSIST-TS prCEN/TS 839:2007
01-september-2007
=DãþLWQDVUHGVWYD]DOHV8JRWDYOMDQMHSUHYHQWLYQHXþLQNRYLWRVWL]DãþLWQLK
VUHGVWHYSURWLJOLYDPRGSUWRWURVQLFDP3RYUãLQVNRQDQDãDQMH
Wood preservatives - Determination of the protective effectiveness against wood
destroying basidiomycetes - Application by surface treatment
Holzschutzmittel - Bestimmung der vorbeugenden Wirksamkeit gegen holzzerstörende
Basidiomyceten - Anwendung mit Oberflächenverfahren
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-a-vis des
champignons basidiomycetes lignivores - Application par traitement de surface
Ta slovenski standard je istoveten z: prCEN/TS 839
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
oSIST-TS prCEN/TS 839:2007 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
TECHNICAL SPECIFICATION
FINAL DRAFT
prCEN/TS 839
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
July 2007
ICS 71.100.50 Will supersede ENV 839:2002
English Version
Wood preservatives - Determination of the protective
effectiveness against wood destroying basidiomycetes -
Application by surface treatment
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis-à-vis des champignons Wirksamkeit gegen holzzerstörende Basidiomyceten -
basidiomycètes lignivores - Application par traitement de Anwendung mit Oberflächenverfahren
surface
This draft Technical Specification is submitted to CEN members for formal vote. It has been drawn up by the Technical Committee CEN/TC
38.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change without notice
and shall not be referred to as a Technical Specification.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. prCEN/TS 839:2007: E
worldwide for CEN national Members.
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prCEN/TS 839:2007 (E)
Contents Page
Foreword.4
Introduction .5
1 Scope .6
2 Normative references .6
3 Terms and definitions .6
4 Principle.6
5 Test materials and apparatus.7
5.1 Biological material.7
5.2 Products and reagents.8
5.3 Apparatus .9
6 Sampling of the preservative.9
7 Test specimens.9
7.1 Species of wood.9
7.2 Wood quality .10
7.3 Provision of the test specimens.10
7.4 Dimensions and density of test specimens.10
7.5 Number and distribution of test specimens .10
8 Procedure .11
8.1 Preparation of the untreated test specimens .11
8.2 Preparation of the treated test specimens.11
8.3 Exposure to fungi .13
8.4 Culture conditions and duration of test .13
8.5 Assessment of test.13
9 Statement of results .14
10 Test report .14
Annex A (informative) Test fungi .16
A.1 General information on maintenance and acquisition of test strains.16
A.2 Maintenance and treatment of test fungi .16
A.3 Information regarding obligatory fungi .17
Annex B (normative) Methods of sterilisation .19
B.1 Ionising radiation.19
B.2 Epoxyethane-based sterilant.19
B.3 Epoxypropane-based sterilant .19
B.4 Steam .20
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Annex C (informative) .21
Examination of colonisation.21
C.5 Statement of results .22
Annex D (informative) Example of a test report.24
Annex E (informative) .27
Environmental, health and safety precautions within chemical/biological laboratory.27
Bibliography.28
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prCEN/TS 839:2007 (E)
Foreword
This document (prCEN/TS 839:2007) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.
This document is currently submitted to the Formal Vote.
This document will supersede ENV 839:2002.
Significant technical differences between this standard and ENV 839:2002 are as follows:
a) change of assessment from visual examination for decay supplemented with culturing to assess
colonisation of the interior, to determination of loss in mass due to fungal decay, as well as visual
examination for decay of the surface and the interior of the test specimens. The visual examination is now
included as an optional means of assessment to determine colonisation and that this requires an
additional series of test specimens (Annex C);
b) addition in 7.5 of treated check test specimens for calculation of the correction value;
c) taking into account of the terms given in EN 1001-1and the definitions of EN 1001-2;
d) Introduction of an informative Annex E which considers minimisation of environmental and health hazards
caused by the use of this biological test.
This standard includes an Annex A, Annex C, Annex D and Annex E that are informative and an Annex B that
is normative.
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prCEN/TS 839:2007 (E)
Introduction
This European Standard specifies a laboratory test method that provides a basis for assessing the
effectiveness of a wood preservative, when applied as a surface treatment against wood destroying
basidiomycetes. It tests whether the applied treatment is able to prevent the penetration of fungi into the test
specimen’s untreated interior under the conditions of test.
This laboratory method provides one criterion by which the effectiveness of a product can be assessed. In
making this assessment, the methods by which the preservative may be applied should be taken into account.
It is also recommended that results from this test should be supplemented by those from other relevant tests
and above all by practical experience.
The procedures described in this standard method are intended to be carried out by suitably trained and/or
supervised specialists.
Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities,
conditioning chambers and special training for personnel. Also see Annex E for environmental, health and
safety precautions.
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prCEN/TS 839:2007 (E)
1 Scope
This European Standard specifies a test method for determining the protective effectiveness of a wood
preservative applied to the surface of the wood, against wood destroying basidiomycetes cultured on an agar
medium.
The method is applicable to all products that are to be applied by superficial application processes. This
includes:
organic solvent-based wood preservatives; or
organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of
concentrates; or
water-soluble products; or
chemicals that are being studied as active ingredients for application by superficial processes.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this European Standard, the following terms and definitions apply.
3.1
representative sample
sample having its physical and/or chemical characteristics identical to the volumetric average characteristics
of the total volume being sampled
[EN 1001-2:2005, 4.71]
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
[Adapted from EN 1001-2:2005, 4.83]
3.3
superficial application process
process that does not include particular features or procedures intended to overcome the natural resistance of
wood to penetration of a wood preservative in its ready to use form
[EN 1001-2:2005, 4.82]
4 Principle
Several series of test specimens of a susceptible wood species are end-sealed with a material to prevent
penetration of the wood preservative under test into the end grain of the test specimens. The end-sealed test
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prCEN/TS 839:2007 (E)
specimens are treated with the wood preservative under test using the process and application rate specified
by the supplier.
NOTE Suitable application methods are brushing, pipetting and dipping.
The treated test specimens are exposed to attack by basidiomycetes in pure culture. The performance of the
test product is assessed in terms of its ability to prevent decay as determined by the maximum acceptable
loss in mass, and the absence of visible decay of the surface and the untreated interior.
5 Test materials and apparatus
5.1 Biological material
5.1.1 General
The test fungi to be used are as follows.
5.1.2 Obligatory fungus in all cases
Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of a minimum of 20 %.
5.1.3 Obligatory fungus for particular hazards
Coriolus versicolor (Linnaeus) Quélet (CTB 863A) on hardwood and/or on softwood as appropriate.
Loss in mass of beech in 16 weeks: a mass fraction of a minimum of 20 %.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of a minimum of 15 %.
5.1.4 Two species to be used compulsorily on the basis of the nature of the test product
For all products except creosote-type products:
Poria placenta (Fries) Cooke sensu J. Eriksson (FPRL 280) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of a minimum of 20 %;
Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of a minimum of 20 %.
For creosotes and similar products:
Lentinus lepideus Fries ex Fries (BAM Ebw. 20) on softwood.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of a minimum of 20 %;
Lentinus cyathiformis (Schaeffer ex Fries) Bresadola (CTB 67-02B) on hardwood.
Loss in mass of beech in 16 weeks: a mass fraction of a minimum of 20 %.
5.1.5 Optional fungi
For specific regional uses or conditions, it is also possible to select other fungi on an optional basis.
NOTE When optional fungi are used, information similar to that given in Annex A for the obligatory fungi that should
be included in the test report.
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prCEN/TS 839:2007 (E)
5.1.6 Maintenance of strains
The strains shall be maintained and treated (frequency of subculturing, alternation of culture media, etc.) in
accordance with the instructions of their laboratory of origin (see A.2). The parent strain shall be maintained in
the laboratory of its origin so as to conserve and to assure its vigour.
If tests are not undertaken regularly or if a strain shows signs of degeneration a new standard culture of the
strain should be obtained from the laboratory of its origin for each test (see A.2). When new strains are
received, the virulence shall be tested to ensure the strain can achieve the minimum loss in mass (see 5.1.2,
5.1.3 and 5.1.4).
5.2 Products and reagents
5.2.1 Culture medium
The culture medium is a malt agar medium with the following composition:
malt extract:
in concentrated form: (50 ± 0,5) g;
in powder form: (40 ± 0,5) g;
agar causing no inhibition of growth of fungi:
(20 ± 0,5) g to (30 ± 0,5) g;
water conforming to grade 3 of EN ISO 3696.
quantity to make up to 1 000 ml.
Prepare this medium by warming the mixture in a boiling water bath or steam bath, stirring until completely
dissolved.
Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of 3 mm to
4 mm when in its in-use position. Close the vessels as specified in 5.3.1 and sterilise in an autoclave at
121 °C for 20 min. Let the vessels cool in their in-use position.
5.2.2 Solvents and diluents
For water soluble or water dispersible preservatives:
water conforming to grade 3 of EN ISO 3696.
For preservatives to be diluted or dissolved in an organic solvent:
suitably volatile liquids that leave no residue in the wood that would have a toxic effect on the fungi at the
end of the post-treatment conditioning period.
NOTE Toluene and xylene of recognized analytical grade have been found suitable.
5.2.3 Fumigant (if necessary)
Xylene technical grade.
5.2.4 End-seal compound
A material resistant to the penetration of the wood preservative under test and the test fungi, or separate
materials for each, and without any fungistatic or fungicidal activity within the test specimen.
NOTE Three brush coats of a 2-component epoxy lacquer, with drying between each application, have been found to
be suitable.
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5.3 Apparatus
5.3.1 Culture vessels, Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml,
2 2
providing a flat surface area of between 85 cm and 120 cm for the medium.
NOTE 1 Examples of suitable vessels are given in EN 113.
NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with leak
proof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad of cotton
wool.
5.3.2 Drying oven, capable of being controlled at (103 ± 2) °C.
5.3.3 Desiccators, with efficient desiccant (silica gel for example).
5.3.4 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.5 Drying supports that will give a minimum contact with the treated test specimens. The supports shall
be of a material that does not react with the test solvent or test wood preservative, for example glass for
organic products.
5.3.6 Culture chamber, (incubator or room), dark and controlled at (22 ± 2) °C and (70 ± 5) % relative
humidity.
5.3.7 Test specimen supports, made of glass, stainless steel or any other inert material, that is to say, with
no risk of having any effect on the culture medium, the fungus, the wood or the test wood preservative, or of
being itself modified. Supports can be capable of holding either one or two test specimens. The supports are
used to prevent direct contact of the test specimens with the culture medium, but shall not separate them from
it by more than 3 mm.
NOTE If abnormally high moisture contents in the test specimens are experienced consistently, use of test specimen
supports of approximately 5 mm thick can help to control the problem. If thicker test specimen supports are used, this
should be recorded in the test report.
5.3.8 Ordinary laboratory equipment, including a balance capable of weighing to the nearest of 0,01 g and
an autoclave.
6 Sampling of the preservative
The sample of the wood preservative shall be representative of the product to be tested. Samples shall be
stored and handled in accordance with any written instructions from the supplier.
NOTE For the sampling of wood preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The species of wood to be used shall be susceptible to attack by fungi and shall be readily penetrated by
liquids.
The reference species are Scots pine (Pinus sylvestris Linnaeus) representing softwoods and beech (Fagus
sylvatica Linnaeus) representing hardwoods.
Additional tests may be undertaken using other species corresponding to the above characteristics, and of
particular importance for certain countries, but if so, this shall be stated in the test report.
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7.2 Wood quality
The wood shall be free from cracks, stain, decay, insect damage or other defects. The wood shall not have
been water-stored, floated, chemically treated or steamed.
NOTE Wood that has been kiln dried at temperatures below 60 °C can be used.
The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual
growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole.
The beech shall be even-grained, free from tyloses and discoloration. It shall have between 2 and 6 annual
growth rings per 10 mm.
7.3 Provision of the test specimens
Prepare planed strips having a cross section of (25 ± 0,5) mm × (15 ± 0,5) mm. The longitudinal faces shall be
parallel to the direction of the grain. The annual rings shall have a contact angle of (45 ± 15) ° to the broad
faces. Make transverse cuts, neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to
have test specimens (50 ± 0,5) mm long.
For treatment, drying and ageing, the test specimens can be kept in planed strips of a length sufficient to
provide one test specimen for exposure to each of the test fungi. Each strip should be end-sealed prior to
treatment.
The specimens shall originate from a minimum of three trees or shall be taken at random from a stock
originally of more than 500 test specimens and originating from at least five planks.
7.4 Dimensions and density of test specimens
The dimensions of each test specimen at a mass fraction of (12 ± 2) % moisture content shall be
(50 ± 0,5) mm x (25 ± 0,5) mm x (15 ± 0,5) mm.
NOTE A moisture meter of the two-pronged electrical conductivity type is suitable for assessing moisture content.
2
The total surface area of the faces to be treated is theoretically 40 cm but an allowance shall be made for any
encroachment of the sealing compound on to these faces.
In a batch of test specimens to be treated, the it is permitted that the density of an individual can differ from
the mean value of the batch by ± 10 %. This tolerance is increased up to ± 20 % for the untreated test
specimens. The mean density for the treated test specimens used for the test shall be recorded in the test
report.
7.5 Number and distribution of test specimens
The test specimens are divided into:
e treated test specimens :
1
these are the treated test specimens subjected to attack by the wood destroying fungi. Use at least
six test specimens for each combination of preservative, quantity to be applied, preservative
concentration, test fungus and for each timber species.
In case of dipping select at least 6 test specimens within a range of 10 % from the target retention.
Supplementary samples shall be treated, in order to have a sufficient number of correctly treated
specimens to put in test.
NOTE The treated test specimens are assessed by visual examination for decay of their surfaces and/or interior by the
test fungi. If optional tests for colonisation of the test fungi are required as an additional method of assessment, this should
be carried out on a parallel series of treated test specimens (Annex C).
e untreated test specimens:
2
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e untreated control test specimens: these are untreated test specimens, equal in number to the
2.1
treated test specimens e and of the same wood species, which are placed one in each culture
1
vessel together with a treated test specimen;
e virulence control test specimens: these are untreated test specimens which are subjected to
2.2
attack by the test fungi to monitor vigour. Use six of these for each combination of test fungus and
timber species used in the test.
— e treated check test specimens for calculation of the correction value:
3
these are test specimens treated in exactly the same way as the e test specimens. Use at least six
1
test specimens for each combination of preservative, quantity to be applied, preservative
concentration and of the same wood species. They are placed, after drying, conditioning and any
appropriate ageing in uninoculated culture vessels, two in each vessel. Variations in mass of these
test specimens make it possible to determine the correction factor (C) of the variations in mass of the
treated test specimens e resulting from factors other than attack by the test fungi. At a given treating
1
concentration, factor C is the mass fraction change of the e test specimens.
3
Mark each specimen so that it can be identified throughout the test.
8 Procedure
8.1 Preparation of the untreated test specimens
Place the numbered untreated test specimens (e and e ) in the oven (5.3.2) and leave them there for 18 h
2.1 2.2
1)
to 24 h . Cool to room temperature in a desiccator (5.3.3) and weigh to the nearest 0,01 g to determine the
initial dry mass (m ). Place the test specimens in the conditioning chamber (5.3.4) until they need to be
0
sterilised (8.3).
NOTE Untreated test specimens are not end-sealed.
8.2 Preparation of the treated test specimens
8.2.1 Preparation
Place the numbered test specimens to be treated (e and e ) in the oven at (103 ± 2) °C (5.3.2) and leave
1 3
1)
them there for 18 h to 24 h . Cool to room temperature in a desiccator (5.3.3) and weigh to the nearest 0,01 g
to determine the initial dry mass (m ). Place the test specimens in the conditioning chamber (5.3.4) until they
0
need to be end-sealed (8.2.2).
1
)
In the case of supplementary tests (7.1) using species of wood other than Scots pine sapwood or beech, this drying
time may need to be longer than 18 h to 24 h; the drying time should be such that the test specimens achieve constant
mass. This can be established by selecting at random from the batch being dried 10 test specimens; after drying and
cooling as directed, determine the total mass, return the test specimens to the oven and repeat the operation at intervals
of not less than 4 h. Constant mass is achieved when the total mass of the selected specimens does not lose more than
0,05 g between weighings.
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8.2.2 End-sealing
Apply the end-sealing compound resistant to the penetration of the test wood preservative (5.2.4) to both end-
grain surfaces of each test specimen to be treated (e and e ). Allow to dry in the conditioning chamber (5.3.4)
1 3
for at least 24 h after the last application.
NOTE Since variation in end seal amount can create errors on mass loss careful operation in applying the end seal
as evenly as possible is required. As quality control guideline a variation within a 10 % limit should be envisaged and
additional absolute mass correction to all specimens could be useful.
8.2.3 Treatment with the test wood preservative
Treat the test specimens e and e on the unsealed longitudinal faces. If application is by brushing or by
1 3
pipette, calculate the amount of test product required to treat each face. Apply the amount evenly to each face
individually and weigh the test specimen before (m ) and after (m ) each application to the nearest 0,01 g.
1 2
Allow to dry between applications. Calculate the uptake of wood preservative solution for each face of each
test specimen (m - m ). Calculate the total uptake for each test specimen and express it in grams of wood
2 1
preservative per square metre of treated surface.
NOTE If the balance has a tare facility, it is easier to tare the balance with the test specimen on it, apply the test
wood preservative, and then record the uptake directly.
If application is by dipping, weigh each test specimen to the nearest 0,01 g (m ), dip for the required time then
1
remove any excess liquid with absorbent paper. Reweigh each test specimen immediately and record the
mass after treatment (m ).
2
Calculate the uptake of wood preservative solution for each test specimen (m - m ) and express it in grams of
2 1
wood preservative per square metre of treated surface.
8.2.4 Drying
Following treatment (8.2.3), place the treated test specimens on drying supports (5.3.5) in the conditioning
chamber (5.3.4). Invert the test specimens twice each week. Dry the test specimens until they weigh in at 24 h
intervals and are within ± 0,01 g.
NOTE 1 The length of the drying period will vary with the nature of the test wood preservative.
If test specimens are to be subjected to an ageing procedure, this shall be carried out after this drying
procedure.
NOTE 2 If the test specimens have been retained as planed strips, th
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