Foodstuff - Detection of irradiated foodstuff containing cellulose by ESR spectroscopy

This European Standard specifies a method for the detection of foods containing cellulose which have been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called electron paramagnetic resonance (EPR) spectrum, of the food.
Interlaboratory studies have been successfully carried out with pistachio nut shells, paprika powder and fresh strawberries. However, it has been shown that false positive results can appear when analysing bleached nuts. For further information, see Clause 7 on limitations.

Lebensmittel - ESR-spektroskopischer Nachweis von bestrahlten cellulosehaltigen Lebensmitteln

Dieses Dokument legt ein Verfahren zum Nachweis von mit ionisierender Strahlung behandelten cellulosehaltigen Lebensmitteln fest. In diesem Verfahren wird das ESR (Elektronen-Spin-Resonanz) – auch bekannt als EPR (Elektronen-Paramagnetische-Resonanz)-Spektrum des Lebensmittels analysiert, siehe [1] bis [13].
Dieses Verfahren wurde im Ringversuch erfolgreich an Pistazienschalen [14] bis [18], an Paprikapulver [19] und [20] und an frischen Erdbeeren [21] geprüft. Es hat sich jedoch gezeigt, dass das Verfahren bei der Untersuchung von gebleichten Nüssen zu falsch positiven Ergebnissen führen kann. Weitere Informationen siehe Abschnitt 7 zu den Grenzen des Verfahrens.

Produits alimentaires - Détection par spectroscopie RPE d'aliments ionisés contenant de la cellulose

Le présent document spécifie une méthode de détection de traitement ionisant appliquée à des produits
alimentaires contenant de la cellulose, par analyse du spectre de résonance de spin électronique (RSE)
aussi appelé spectre de résonance paramagnétique électronique (RPE) des aliments ; voir [1] à [13].
Des essais interlaboratoires ont été réalisés sur des écales de pistache [14] à [18], du paprika en poudre
[19] et [20], et des fraises fraîches [21]. Toutefois, il a été démontré que des résultats faux positifs peuvent
être obtenus lors de l’analyse de noix en coque blanchies. Pour de plus amples informations, voir
l’Article 7 relatif aux limites.

Živila - Določevanje obsevanosti živil, ki vsebujejo celulozo, s spektroskopijo ESR

Ta evropski standard določa metodo za določevanje živil, ki vsebujejo celulozo in so bila obdelana z ionizirajočim sevanjem. Določevanje poteka z analizo elektronske spin resonančne spektroskopije (ESR), imenovane tudi elektronske paramagnetne resonance (EPR), živil.
Medlaboratorijske študije so bile uspešno izvedene na lupinah pistacijevih oreščkov, papriki v prahu in svežih jagodah. Pri analizi beljenih oreščkov se je izkazalo, da lahko pride do lažno pozitivnih rezultatov. Za več informacij glejte točko 7 v zvezi z omejitvami.

General Information

Status
Published
Public Enquiry End Date
19-Dec-2019
Publication Date
12-May-2022
Current Stage
6060 - National Implementation/Publication (Adopted Project)
Start Date
04-May-2022
Due Date
09-Jul-2022
Completion Date
13-May-2022

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SLOVENSKI STANDARD
SIST EN 1787:2022
01-junij-2022
Nadomešča:
SIST EN 1787:2001
Živila - Določevanje obsevanosti živil, ki vsebujejo celulozo, s spektroskopijo ESR
Foodstuff - Detection of irradiated foodstuff containing cellulose by ESR spectroscopy
Lebensmittel - ESR-spektroskopischer Nachweis von bestrahlten cellulosehaltigen
Lebensmitteln
Produits alimentaires - Détection par spectroscopie RPE d'aliments ionisés contenant de
la cellulose
Ta slovenski standard je istoveten z: EN 1787:2022
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
SIST EN 1787:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 1787:2022

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SIST EN 1787:2022


EN 1787
EUROPEAN STANDARD

NORME EUROPÉENNE

April 2022
EUROPÄISCHE NORM
ICS 67.050 Supersedes EN 1787:2000
English Version

Foodstuff - Detection of irradiated foodstuff containing
cellulose by ESR spectroscopy
Produits alimentaires - Détection par spectroscopie Lebensmittel - ESR-spektroskopischer Nachweis von
RPE d'aliments ionisés contenant de la cellulose bestrahlten cellulosehaltigen Lebensmitteln
This European Standard was approved by CEN on 14 February 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 1787:2022 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
SIST EN 1787:2022
EN 1787:2022 (E)
Contents Page
European foreword . 3
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Apparatus and equipment . 6
6 Procedure . 6
6.1 Sample preparation . 6
6.1.1 General. 6
6.1.2 Shells and stones . 6
6.1.3 Spices . 6
6.1.4 Strawberries . 7
6.2 ESR Spectroscopy . 7
6.2.1 Spectrometer settings . 7
6.2.2 Analysis of sample . 8
7 Evaluation . 8
7.1 G-value calculation . 8
7.2 Identification of irradiated samples . 8
8 Limitations . 9
9 Validation . 9
10 Test report . 10
Annex A (informative) Example Figures . 11
Annex B (informative) Further information on the applicability . 13
Bibliography . 14
2

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SIST EN 1787:2022
EN 1787:2022 (E)
European foreword
This document (EN 1787:2022) has been prepared by Technical Committee CEN/TC 275 “Food analysis -
Horizontal methods”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2022, and conflicting national standards shall
be withdrawn at the latest by October 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 1787:2000.
The predecessor of this document was elaborated on the basis of a protocol developed following a
concerted action supported by the Commission of European Union (XII C.5). Experts and laboratories
from EU and EFTA countries, contributed jointly to the development of this protocol.
In comparison with the previous edition, the entire document was editorially revised according to
current rules. Additionally, the following technical modifications have been made:
a) the scope was supplemented by the information, that chemical bleaching of nuts in shells can lead to
comparable signals;
b) clause “Terms and definitions” was added;
c) scientific language usage of clause “Principle” had been clarified;
d) former 4.1, 4.2 and 4.7 were updated and a footnote added for the g-value calculation;
e) clause “Procedure” was scientifically refined, its normative character (i.e. provisions set out)
modified towards more exemplary/suggestive expressions of provision and aligned with EN 13708;
f) subclause “General” was added to subclause “Sample preparation”;
g) clause “Evaluation” was amended by supplementing two subclauses, 7.1 “G-value calculation” and
7.2 “Identification of irradiated samples”, in alignment with EN 13708, including implementing its
former content into 7.2, whereas “6,0 mT” was refined to “6,05 mT ± 0,05 mT”;
h) clause “Limitations” was extended;
i) Figures A.5 and A.7 of irradiated but bleached nutshells from hazelnuts and walnuts and Figures A.6
and A.8 of irradiated (not bleached) nutshells from hazelnuts and walnuts were added;
j) Annex B was extended by the matrix hazelnuts;
k) the Bibliography was updated and extended by entry [23], [24] and [25].
3

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SIST EN 1787:2022
EN 1787:2022 (E)
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
4

---------------------- Page: 6 ----------------------
SIST EN 1787:2022
EN 1787:2022 (E)
1 Scope
This document specifies a method for the detection of foodstuff containing cellulose which have been
treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called
electron paramagnetic resonance (EPR) spectrum, of the foodstuff, see [1] to [13].
Interlaboratory studies have been successfully carried out with pistachio nut shells, [14] to [18], paprika
powder [19] and [20] and fresh strawberries [21]. However, it has been shown that chemical bleaching
of nuts in shells can lead to comparable signals. For further information, see Clause 8 on limitations.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696)
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
4 Principle
ESR spectroscopy detects paramagnetic centres (e.g. radicals). They are either due to irradiation, or to
other compounds present. An intense external magnetic field produces a difference between the energy
levels of the electron spins m = +½ and m = −½, leading to resonance absorption of an applied
s s
microwave beam in the spectrometer. ESR spectra are conventionally displayed as the first derivative of
the absorption with respect to the applied magnetic field.
The magnetic field and microwave frequency values depend on the experimental arrangements (sample
size and sample holder), while their ratio (i.e. g value) is an intrinsic characteristic of the paramagnetic
centre and its local co-ordination. For further information, see [1] to [13].
Radiation treatment produces specific radicals that can be mostly detected in solid and dry parts of the
foodstuff. The intensity of the signal obtained increases with the concentration of the paramagnetic
compounds and thus with the applied dose.
5

---------------------- Page: 7 ----------------------
SIST EN 1787:2022
EN 1787:2022 (E)
5 Apparatus and equipment
Usual laboratory apparatus and, in particular, the following:
5.1 Commercially available X-Band ESR spectrometer including magnet (electro or permanent),
1
microwave bridge, console with field- controller and signal-channel, rectangular or cylindrical cavity .
®2
5.2 ESR tubes, suitable for the ESR spectrometer used (e.g. Suprasil quartz tubes).
5.3 Balance, accuracy of 1 mg (optional).
5.4 Laboratory vacuum oven or freeze dryer.
5.5 Electric blender.
5.6 Filter paper.
5.7 Scalpel, pincers.
5.8 Water complying with at least grade 3 of EN ISO 3696.
6 Procedure
6.1 Sample preparation
6.1.1 General
Do not grind the samples since grinding could either diminish the signal to noise ratio and also cause a
change of the shape of the ESR spectrum or induce other ESR signals [25].
6.1.2 Shells and stones
Remove pieces of suitable size (about 50 mg to 100 mg, e.g. 3,0 mm to 3,5 mm in diameter) from the shells
or stones of the foodstuff, e.g. using a scalpel or pincers. Drying [e.g. in a freeze-dryer or at approximately
40 °C in a laboratory vacuum oven (5.4)] is usually not necessary in the case of nutshells but
recommended for pips and kernels of fruits.
6.1.3 Spices
For example, use about 150 mg to 200 mg of the spice sample. Drying (e.g. in a freeze-dryer or at
approximately 40 °C in a laboratory vacuum oven (5.4)) is usually not necessary.

1
g-value calculation unit including frequency counter magnetic field probe (magnetic resonance Teslameter) or
any other built in g-value calculation unit.
2 ®
Suprasil is an example of a product available commercially. This information is given for the convenience
of users of this Standard and does not constitute an endorsement of CEN or CENELEC of this product.
6

---------------------- Page: 8 ----------------------
SIST EN 1787:2022
EN 1787:2022 (E)
6.1.4 Strawberries
Strawberry samples should be measured immediately after receipt. Otherwise store the samples at
approximately −18 °C until analysis.
For ESR measurement about 200 mg of seeds (achenes) of strawberries are needed. These can be gained
usually from about 80 g of strawberries.
For separation of the small seeds from the main fruit body either peel off the skin (recommendation: in
frozen state) or use the whole fruit (without stalks and leaves). Homogenize the strawberries in an
electric blender (5.5). Add 500 ml of water to the fruit pulp and stir thoroughly. Allow the seeds to settle
and decant most of the water together with the floating fruit pulp. Repeat this procedure once or twice to
remove any remaining fruit pulp.
Place the seeds on filter paper to remove adhering water. Dry the seeds in a freeze dryer or at
approximately 40 °C in a laboratory vacuum oven (5.4) e.g. for 2 h.
Storing samples in the frozen state will not adversely affect the detection of treatment with radiation.
6.2 ESR Spectroscopy
6.2.1 Spectrometer settings
The parameters shown in Table 1 have been found to be successful in interlaboratory tests (see Clause 9).
The values shown (Table 1) are given as examples and should be optimized per sample and ESR
spectrometer as required.
Use a time constant and sweep rate (or sweep time) appropriate for an ESR signal with a peak to peak
linewidth of approximately 0,8 mT.
Table 1 — Example for ESR spectrometer settings
Parameter Setting
a
Microwave radiation:
Frequency
...

SLOVENSKI STANDARD
oSIST prEN 1787:2019
01-december-2019
Živila - Detekcija obsevane hrane, ki vsebuje celulozo, s spektroskopijo ESR
Foodstuffs - Detection of irradiated food containing cellulose by ESR spectroscopy
Lebensmittel - ESR-spektroskopischer Nachweis von bestrahlten cellulosehaltigen
Lebensmitteln
Produits alimentaires - Détection par spectroscopie RPE d'aliments ionisés contenant de
la cellulose
Ta slovenski standard je istoveten z: prEN 1787
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
oSIST prEN 1787:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 1787:2019

---------------------- Page: 2 ----------------------
oSIST prEN 1787:2019


DRAFT
EUROPEAN STANDARD
prEN 1787
NORME EUROPÉENNE

EUROPÄISCHE NORM

October 2019
ICS Will supersede EN 1787:2000
English Version

Foodstuffs - Detection of irradiated food containing
cellulose by ESR spectroscopy
Produits alimentaires - Détection par spectroscopie Lebensmittel - ESR-spektroskopischer Nachweis von
RPE d'aliments ionisés contenant de la cellulose bestrahlten cellulosehaltigen Lebensmitteln
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 275.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 1787:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 1787:2019
prEN 1787:2019 (E)
Contents Page
European foreword .3
1 Scope .4
2 Normative references .4
3 Terms and definitions .4
4 Principle .4
5 Apparatus and equipment .5
6 Procedure .5
6.1 Sample preparation .5
6.1.1 General .5
6.1.2 Shells and stones .5
6.1.3 Spices .5
6.1.4 Strawberries .6
6.2 ESR Spectroscopy .6
6.2.1 Spectrometer settings .6
6.2.2 Analysis of sample .7
7 Evaluation .7
8 Limitations .7
9 Validation .8
10 Test report .9
Annex A (normative) Figures. 10
Annex B (informative) Further information on the applicability . 12
Bibliography . 13

2

---------------------- Page: 4 ----------------------
oSIST prEN 1787:2019
prEN 1787:2019 (E)
European foreword
This document (prEN 1787:2019) has been prepared by Technical Committee CEN/TC 275 “Food
analysis - Horizontal methods”, the secretariat of which is held by DIN.
This document will supersede EN 1787:2000.
The predecessor of this document was elaborated on the basis of a protocol developed following a
concerted action supported by the Commission of European Union (XII C.5). Experts and
laboratories from EU and EFTA countries, contributed jointly to the development of this protocol.
The changes between this document and the previous edition are as follows:
— The scope contains the information that it has been shown that false-positive results can appear
when analysing bleached nuts.
— The clause on limitations was amended by the information that, having identified, that the
method yields false positives with certain matrices (e.g. bleached nuts), it is in the responsibility
of the analyst to ensure that the method is fit for purpose on the matrix on which it is applied.
— The clause on limitations was furthermore amended by the information that it has been shown
that with nuts in shells (hazelnuts, walnuts, pistachio), a bleaching can lead to comparable
positive signals which can be mixed up with radiation induced signals.
— The Figures A.5 to A.8 were included to show how figures from non-irradiated but bleached
nutshells can be differentiated from irradiated shells from hazelnuts and walnuts.
— This new version was editorially updated according to current rules.
3

---------------------- Page: 5 ----------------------
oSIST prEN 1787:2019
prEN 1787:2019 (E)
1 Scope
This document specifies a method for the detection of foods containing cellulose which have been
treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also called
electron paramagnetic resonance (EPR) spectrum, of the food, see [1] to [13].
Interlaboratory studies have been successfully carried out with pistachio nut shells, [14] to [18],
paprika powder [19] and [20] and fresh strawberries [21]. However, it has been shown that false-
positive results can appear when analysing bleached nuts. For further information, see Clause 7 on
limitations.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments)
applies.
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
• IEC Electropedia: available at http://www.electropedia.org/
• ISO Online browsing platform: available at http://www.iso.org/obp
4 Principle
ESR spectroscopy detects paramagnetic centres (e.g. radicals). They are either due to irradiation, or
to other compounds present. An intense external magnetic field produces a difference between the
energy levels of the electron spins ms = + 1/2 and ms = −1/2, leading to resonance absorption of an
applied microwave beam in the spectrometer. ESR spectra are conventionally displayed as the first
derivative of the absorption with respect to the applied magnetic field.
The field and frequency values depend on the experimental arrangements (sample size, sample
holder and spectrometer specifications), while their ratio (i.e. g value) is an intrinsic characteristic
of the paramagnetic centre and its local coordination. For further information, see [1] to [13].
Radiation treatment produces specific radicals which can be mostly detected in solid and dry parts
of the food. The intensity of the signal obtained increases with the concentration of the
paramagnetic compounds and thus with the applied dose.
4

---------------------- Page: 6 ----------------------
oSIST prEN 1787:2019
prEN 1787:2019 (E)
5 Apparatus and equipment
Usual laboratory apparatus and, in particular, the following:
5.1 Commercially available X-Band ESR spectrometer including magnet (electro or
permanent), microwave bridge, console with field- controller and signal-channel, rectangular or
cylindrical cavity.
®1
5.2 ESR tubes, suitable for the ESR spectrometer used (e.g. Suprasil quartz tubes,)
5.3 Balance, accurate to the nearest 1 mg (optional)
5.4 Laboratory vacuum oven or freeze dryer
5.5 Electric blender
5.6 Filter paper
5.7 Scalpel, pincers
5.8 Water of at least grade 3 according to EN ISO 3696
6 Procedure
6.1 Sample preparation
6.1.1 General
Do not grind the samples since grinding could either diminish the signal to noise ratio and can also
cause a change of the shape of the ESR spectrum or induce other ESR signals [25].
6.1.2 Shells and stones
Remove pieces of suitable size (about 50 mg to 100 mg, e.g. 3,0 mm to 3,5 mm in diameter) from the
shells or stones of the food, e.g. using a scalpel or pincers. Drying (e.g. in a freeze-dryer or at
approximately 40 °C in a laboratory vacuum oven (5.4)) is usually not necessary in the case of
nutshells but recommended for pips and kernels of fruits.
6.1.3 Spices
For example use about 150 mg to 200 mg of the spice sample. Drying (e.g. in a freeze-dryer or at
approximately 40 °C in a laboratory vacuum oven (5.4)) is usually not necessary.

1 ®
) Suprasil is an example of a product available commercially. This information is given for the
convenience of users of this Standard and does not constitute an endorsement of CEN of this product.
5

---------------------- Page: 7 ----------------------
oSIST prEN 1787:2019
prEN 1787:2019 (E)
6.1.4 Strawberries
Strawberry samples should be measured immediately after receipt. Otherwise store the samples at
approximately - 18 °C until analysis.
For ESR measurement about 200 mg of seeds (achenes) of strawberries are needed. These can be
gained usually from about 80 g of strawberries.
For separation of the small seeds from the main fruit body either peel off the skin (recommendation:
in frozen state) or use the whole fruit (without stalks and leaves). Homogenize the strawberries in
an electric blender (5.5). Add 500 ml of water to the fruit pulp and stir thoroughly. Allow the seeds
to settle and decant most of the water together with the floating fruit pulp. Repeat this procedure
once or twice to remove any remaining fruit pulp.
Place the seeds on filter paper to remove adhering water. Dry the seeds in a freeze dryer or at
approximately 40 °C in a laboratory vacuum oven (5.4) e.g. for 2 h.
Storing samples in the frozen state will not adversely affect the detection of treatment with
radiation.
6.2 ESR Spectroscopy
6.2.1 Spectrometer settings
These following parameters have been proven to be successful in the interlaboratory test (see
Clause 8). They should be optimized as per sample and ESR spectrometer specifications.
Use a time constant and sweep rate (or sweep time) appropriate for an ESR signal with a peak to
peak linewidth of approximately 0,8 mT. For example, the following ESR spectrometer settings have
been found to be satisfactory:
2
Microwave radiation: Frequency 9,78 GHz , power 0,4 mW (for e.g. pistachio nuts), to
3)
0,8 mW (for e.g. paprika powder or strawberries) ;
2
Magnetic field: 348 mT centre field ), sweep width 20 mT;
Signal channel: 50 kHz or 100 kHz modulation frequency;
 0,4 mT to 1,0 mT modulation amplitude;
4 −1
 100 ms to 200 ms time con
...

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