EN 17958:2024
(Main)Food authenticity - Determination of the δ13C value of mono- (fructose and glucose), di-, and trisaccharides in honey by liquid chromatography-isotope ratio mass spectrometry (LC-IRMS)
Food authenticity - Determination of the δ13C value of mono- (fructose and glucose), di-, and trisaccharides in honey by liquid chromatography-isotope ratio mass spectrometry (LC-IRMS)
This document specifies a method for the determination of the ratio of stable isotopes of carbon (13C/12C) of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass spectrometer (LC-IRMS) for compound separation and subsequent determination of the 13C/12C ratio of mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them to guidance values of genuine honey, which have been previously agreed by subject matter experts, as the 13C/12C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from starch-rich plants or from sugar cane or sugar beet) differ to a certain extent.
The compliance assessment process is not part of this document.
Lebensmittelauthentizität - Bestimmung des δ13C-Wertes von Mono- (Fructose und Glucose), Di- und Trisacchariden in Honig durch Flüssigchromatographie-Isotopenverhältnis-Massenspektrometrie (LC‑IRMS)
Dieses Dokument legt ein Verfahren zur Bestimmung des Verhältnisses stabiler Kohlenstoffisotope (13C/12C) von in Honig enthaltenen Zuckern mittels Flüssigchromatographie gekoppelt mit einem Isotopenverhältnis-Massenspektrometer (LC-IRMS) zur Trennung der Verbindungen und anschließenden Bestimmung des 13C/12C-Verhältnisses von Mono-, Di- und Trisacchariden fest. Diese Verhältnisse können zur Beurteilung der Authentizität von Honig verwendet werden, indem sie mit den Leitwerten für echten Honig verglichen werden, die zuvor von Fachexperten vereinbart wurden, da sich die 13C/12C-Verhältnisse von Zuckern in echtem Honig und Zuckern in Verfälschungsmitteln (Sirupe aus stärkereichen Pflanzen oder aus Zuckerrohr oder Zuckerrüben) in gewissem Maße unterscheiden.
Der Prozess der Konformitätsbewertung ist nicht Teil dieses Dokuments.
Authenticité des aliments - Détermination de la valeur du δ13C des mono- (fructose et glucose), di-, et trisaccharides présents dans le miel par chromatographie en phase liquide spectrométrie de masse de rapports isotopiques (CL-SMRI)
Le présent document spécifie une méthode de détermination du rapport des isotopes stables du carbone (13C/12C) des sucres contenus dans le miel par chromatographie en phase liquide couplée à la spectrométrie de masse de rapports isotopiques (CL-SMRI). La méthode présente la séparation des composés et la détermination des teneurs en 13C/12C des mono-, di- et trisaccharides. Ces rapports isotopiques peuvent être utilisés pour évaluer l’authenticité du miel par comparaison avec des valeurs recommandées pour le miel authentique et convenues par des spécialistes du sujet, car les rapports 13C/12C des sucres du miel authentique, et ceux des sucres contenus dans les produits adultérants du miel (sirops fabriqués à partir de plantes riches en amidon ou à partir de sucre de canne ou de betterave) sont, dans une certaine mesure, différents les uns des autres.
Le processus d’évaluation de la conformité n’est pas traité dans le présent document.
Pristnost živil - Določanje vrednosti δ13C mono- (fruktoza in glukoza), di- in trisaharidov v medu s tekočinsko kromatografijo - z masno spektrometrijo z izotopskim razmerjem (LC-IRMS)
Ta dokument določa metodo za določevanje razmerja stabilnih izotopov ogljika (13C/12C) sladkorjev v medu s tekočinsko kromatografijo z masno spektrometrijo z izotopskim razmerjem (LC-IRMS) za ločevanje spojin in naknadno določanje razmerja 13C/12C mono-, di- in trisaharidov. Ta razmerja je mogoče uporabiti za ocenjevanje pristnosti medu s primerjavo z objavljenimi orientacijskimi vrednostmi pristnega medu, saj se razmerja 13C/12C sladkorjev pristnega medu in sladkorjev, ki jih vsebujejo dodatki (sirupi iz rastlin, bogatih s škrobom, ali iz sladkornega trsa ali sladkorne pese), do določene mere razlikujejo. Postopek ocenjevanja skladnosti ni del tega dokumenta.
General Information
Overview
EN 17958:2024 specifies a laboratory method for assessing food authenticity of honey by measuring compound-specific carbon isotope ratios. The standard describes the determination of the δ13C value (13C/12C) of mono- (fructose, glucose), di- and trisaccharides in honey using liquid chromatography coupled to isotope ratio mass spectrometry (LC‑IRMS). Measured sugar δ13C values are compared with guidance values for genuine honey to help detect adulteration with sugar syrups derived from starch-rich plants, sugar cane (C4) or sugar beet (C3). (Compliance assessment is explicitly not covered.)
Key topics and technical requirements
- Scope and principle: Separation of honey sugars by LC, online oxidation/combustion to CO2, and measurement of CO2 isotopologues (m/z 44, 45, 46) by IRMS to calculate δ13C.
- Sample preparation: Dilution with water, filtration and injection to LC; sequence design includes reference materials, blanks and QC samples.
- Instrumentation & interface:
- LC system and column suitable for mono/di/tri‑saccharide separation using water as mobile phase (polymeric styrene‑divinylbenzene Ca2+ columns are example).
- LC‑IRMS interface for chemical oxidation or combustion to CO2.
- IRMS with collectors for m/z 44/45/46; correction for m/z 45 contributions.
- Calibration & reference materials:
- Use at least two certified reference materials with δ13C values traceable to VPDB for two‑point linear normalization.
- Working gases (CO2, He) and high‑purity reagents required.
- Performance, precision & QA:
- Instrument vendor recommendations are followed for setup; Annexes provide interlaboratory conditions, example chromatograms and precision data.
- EA‑IRMS is cited as complementary; LC‑IRMS improves detection of some C3-derived adulterants and increases sensitivity to C4 sugars (EA‑IRMS can detect additions down to ~7%).
Applications and users
- Applications:
- Detection of honey adulteration with exogenous sugars (C3 and C4 origins).
- Compound‑specific isotopic profiling of honey sugars for quality control, forensic analysis and research.
- Who uses it:
- Official food control and regulatory laboratories.
- Accredited testing labs and proficiency schemes.
- Honey producers and quality assurance teams.
- Research institutions and standardization bodies developing authenticity protocols.
Related standards and references
- Complements bulk stable carbon isotope ratio analysis (EA‑IRMS) and methods harmonized by the International Honey Commission.
- Builds on prior LC‑IRMS applications (e.g., OIV LC‑IRMS methods for wine sugars).
- Traceability tied to VPDB and certified reference materials.
Keywords: EN 17958:2024, food authenticity, honey, δ13C, LC‑IRMS, isotope ratio mass spectrometry, sugar adulteration, stable carbon isotope ratio, VPDB.
Frequently Asked Questions
EN 17958:2024 is a standard published by the European Committee for Standardization (CEN). Its full title is "Food authenticity - Determination of the δ13C value of mono- (fructose and glucose), di-, and trisaccharides in honey by liquid chromatography-isotope ratio mass spectrometry (LC-IRMS)". This standard covers: This document specifies a method for the determination of the ratio of stable isotopes of carbon (13C/12C) of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass spectrometer (LC-IRMS) for compound separation and subsequent determination of the 13C/12C ratio of mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them to guidance values of genuine honey, which have been previously agreed by subject matter experts, as the 13C/12C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from starch-rich plants or from sugar cane or sugar beet) differ to a certain extent. The compliance assessment process is not part of this document.
This document specifies a method for the determination of the ratio of stable isotopes of carbon (13C/12C) of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass spectrometer (LC-IRMS) for compound separation and subsequent determination of the 13C/12C ratio of mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them to guidance values of genuine honey, which have been previously agreed by subject matter experts, as the 13C/12C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from starch-rich plants or from sugar cane or sugar beet) differ to a certain extent. The compliance assessment process is not part of this document.
EN 17958:2024 is classified under the following ICS (International Classification for Standards) categories: 67.180.10 - Sugar and sugar products; 67.180.20 - Starch and derived products. The ICS classification helps identify the subject area and facilitates finding related standards.
You can purchase EN 17958:2024 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2025
Pristnost živil - Določanje vrednosti δ13C mono- (fruktoza in glukoza), di- in
trisaharidov v medu s tekočinsko kromatografijo - z masno spektrometrijo z
izotopskim razmerjem (LC-IRMS)
Food authenticity - Determination of the δ13C value of mono- (fructose and glucose), di-,
and trisaccharides in honey by liquid chromatography-isotope ratio mass spectrometry
(LC-IRMS)
Lebensmittelauthentizität - Bestimmung des δ13C-Wertes von Mono- (Fructose und
Glucose), Di- und Trisacchariden in Honig durch Flüssigchromatographie-
Isotopenverhältnis-Massenspektrometrie (LC-IRMS)
Authenticité des aliments - Détermination de la valeur du δ13C des mono- (fructose et
glucose), di-, et trisaccharides présents dans le miel par chromatographie en phase
liquide spectrométrie de masse de rapports isotopiques (CL-SMRI)
Ta slovenski standard je istoveten z: EN 17958:2024
ICS:
67.180.10 Sladkor in sladkorni izdelki Sugar and sugar products
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17958
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 67.180.10; 67.180.20
English Version
Food authenticity - Determination of the δ C value of
mono- (fructose and glucose), di-, and trisaccharides in
honey by liquid chromatography-isotope ratio mass
spectrometry (LC-IRMS)
Authenticité des aliments - Détermination de la valeur Lebensmittelauthentizität - Bestimmung des δ C -
du δ C des mono- (fructose et glucose), di-, et Wertes von Mono- (Fructose und Glucose), Di- und
trisaccharides présents dans le miel par Trisacchariden in Honig durch
chromatographie en phase liquide spectrométrie de Flüssigchromatographie-Isotopenverhältnis-
masse de rapports isotopiques (CL-SMRI) Massenspektrometrie (LC-IRMS)
This European Standard was approved by CEN on 24 June 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N
EUROPÄISCHES KOMITEE FÜR NORMUN G
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17958:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Reagents . 6
6 Apparatus . 7
7 Procedure. 8
7.1 Preparation of the test sample . 8
7.2 Preparation of the test sample solution . 8
7.3 LC-IRMS setup and performance qualification . 8
7.3.1 LC-IRMS interface setup . 8
7.3.2 IRMS calibration . 8
7.4 Determination and analytical sequence . 8
7.5 Calculations . 8
7.5.1 General . 8
7.5.2 Calculation of compound specific δ C values using two-point linear normalization . 8
8 Precision . 9
8.1 General . 9
8.2 Repeatability . 9
8.3 Reproducibility . 9
Annex A (informative) Experimental conditions used by the participants in the
interlaboratory comparison .10
Annex B (informative) Example of a chromatogram of the expected separation of the honey
saccharides .11
Annex C (informative) Precision data .12
Bibliography .16
European foreword
This document (EN 17958:2024) has been prepared by Technical Committee CEN/TC 460 “Food
Authenticity”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
Honey is a natural sweet substance produced by honey bees having attractive sensory properties.
Demand has increased over the years, partially due to population increase, but also due to the preference
of consumers for natural and unprocessed food. It is a globally traded commodity following complex trade
routes, which makes quality and authenticity control difficult. Honey is among the commodities most
[1]
vulnerable to fraud . The EU Honey Directive lays down the composition and authenticity criteria for
[2] [3]
honey . A set of analytical methods have been harmonized by the International Honey Commission ,
which allows enforcement of the provisions of the Directive. Those methods are widely used but are not
always appropriate for assessing the authenticity of the product: specifically, adulteration of honey by
non-declared dilution with foreign sugars/sweeteners, which is among the most frequently encountered
cases.
Syrups that mimic the composition of honey that are produced by chemical and/or enzymatic
[4]
modification of starch or sucrose are difficult to detect . If the starting product is obtained from a plant
using the Hatch–Slack pathway for carbon fixation (C4 plant), such as maize or sugar cane, stable carbon
isotope ratio analysis (SCIRA) using a combination of an elemental analyser and an isotope ratio mass
[5]
spectrometer (EA-IRMS) offers a possibility to detect additions down to a level of 7 % . Sugars
originating from C3 plants, which use the Calvin–Benson cycle, such as beet root or sugars generated from
rice or wheat starch escape detection by SCIRA. Combining liquid chromatography (LC) with IRMS (LC-
IRMS) offers new possibilities for detecting honey adulteration with sugars derived from C3 plants as
[6][7]
well as increasing the sensitivity for detecting C4 sugars . The method has gained popularity but has
never been subjected to multi-laboratory validation, which is a prerequisite for further developing it into
a standard by a Standards Developing Organization.
13 12
An LC-IRMS method for the determination of the C/ C isotope delta values of glucose, fructose,
glycerol and ethanol in products of viti-vinicultural origin was collaboratively studied by the
International Organization of Vine and Wine (OIV) and endorsed for inclusion in the Compendium of
International Methods of Analysis of Wines and Musts (OIV-OENO resolution 479-2017).
This document provides the basis for the analytical method. The setup of the required apparatus depends
to a large extent on its design principles, and the specific recommendations of the manufacturers should
be followed. It is intended to serve as a frame in which the analyst can define their own analytical work
in accordance with the standard procedure.
1 Scope
13 12
This document specifies a method for the determination of the ratio of stable isotopes of carbon ( C/ C)
of sugars contained in honey by using liquid chromatography coupled to an isotope ratio mass
13 12
spectrometer (LC-IRMS) for compound separation and subsequent determination of the C/ C ratio of
mono-, di-, and trisaccharides. These ratios can be used to assess honey authenticity by comparing them
to guidance values of genuine honey, which have been previously agreed by subject matter experts, as
13 12
the C/ C ratios of sugars of genuine honey and sugars contained in adulterants (syrups made from
starch-rich plants or from sugar cane or sugar beet) differ to a certain extent.
The compliance assessment process is not part of this document.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
isotope delta
δ
stable isotope ratio of a sample expressed relative to a reference
Note 1 to entry: For carbon, this expression is given in Formula (1):
13 12
R C/ C
)
sample(
13 12
(1)
δ C/ C − 1
)
ref(
13 12
R C/ C
)
reference(
13 12 13
Note 2 to entry: The term 𝛿𝛿 ( C/ C) is often changed from the IUPAC format to 𝛿𝛿 C ; this document uses the
ref ref
IUPAC format for familiarity.
Note 3 to entry: To ensure international comparability of isotope delta values, a common reference is used; this
reference is an international measurement standard assigned by convention with isotope delta value exactly equal
to zero.
Note 4 to entry: Carbon isotope delta values for natural isotopic abundance in food materials are small and
expressed in permille (‰) rather than in their native form.
3.2
Vienna Peedee Belemnite
VPDB
international measurement standard for 𝛿𝛿 C
Note 1 to entry: VPDB is a virtual carbonate.
=
Note 2 to entry: The carbon isotopic composition of VPDB is defined by the exact isotope delta value assigned to the
calcium carbonate reference material NBS 19 of d13CVPDB = +1,95‰.
3.3
mass-to-charge ratio
m/z
dimensionless quantity formed by dividing the mass number of an ion by its charge number
3.4
working gas
gas consisting of the same molecule as the analyte gas (i.e. CO ) but introduced directly into the mass
spectrometer from a high-pressure cylinder rather than being created from the sample
3.5
sequence
continuous set of analyses including reference materials for normalization, QA/QC materials, procedural
blanks and samples prepared and analyzed together
4 Principle
Honey is diluted with water, filtered and injected into a liquid chromatography system for separation into
mono-, di-, tri- saccharides. The column effluent is fed into an interface where organic compounds are
oxidized to CO . CO isotopologues with m/z 44, 45 and 46 are separated in the spectrometer and
2 2
detected using Faraday cups. Compound specific δ C (‰) values are then calculated according to
Formula (1).
5 Reagents
Unless otherwise stated, use only reagents of recognized analytical grade (purity ≥ 99 %) and ultrapure
−1
water with a resistivity > 18 MΩ cm .
5.1 Working gas, carbon dioxide, 99,995 %.
5.2 Helium, 99,999 %.
5.3 Certified reference materials, at least two reference materials with certified 𝛿𝛿 C values
traceable to VPDB and spanning the expected measurement range shall be used for calibration . When
used for calibration, the δ C values and the associated uncertainties stated on the accompanying
certificates shall be used. Aqueous solution of those reference materials shall be prepared and used for
calibration according to the specifications of the instrument vendor.
5.4 Reference materials, sugars such as fructose, glucose, or sucrose of analytical grade
(purity > 98 %
...
Die Norm EN 17958:2024 bietet einen klaren und präzisen Rahmen für die Bestimmung des δ13C-Wertes von Mono-, Di- und Trisacchariden in Honig durch flüssige Chromatographie gekoppelt mit Isotopenverhältnis-Massenspektrometrie (LC-IRMS). Diese Methodik ist insbesondere relevant für die Überprüfung der Authentizität von Honig und stellt sicher, dass durch verlässliche wissenschaftliche Methoden die Reinheit und Qualität des Produkts bestätigt werden können. Ein herausragendes Merkmal dieser Norm ist ihr methodischer Ansatz zur Analyse der stabilen Kohlenstoffisotope (13C/12C) in Zuckern. Durch die Anwendung von LC-IRMS zur Trennung von Verbindungen und zur anschließenden Bestimmung des 13C/12C-Verhältnisses wird eine hohe Genauigkeit und Präzision erreicht. Diese analytische Genauigkeit ist entscheidend, um zwischen echtem Honig und adulterierten Proben, beispielsweise solchen, die aus stärkehaltigen Pflanzen oder Zuckerrüben hergestellt wurden, unterscheiden zu können. Darüber hinaus ermöglicht die Norm eine fundierte Bewertung der Honigauthentizität, indem die ermittelten Isotopenverhältnisse mit zuvor definierten Richtwerten für echten Honig verglichen werden. Dies unterstützt nicht nur die Qualitätssicherung in der Honigindustrie, sondern leistet auch einen wichtigen Beitrag zum Verbraucherschutz und zur Aufrechterhaltung des Vertrauens in die Lebensmittelversorgung. Die Relevanz der Norm wird durch das zunehmende Interesse an Lebensmittelsicherheit und Authentizitätsprüfungen in der heutigen Gesellschaft unterstrichen. Die Verwendung von LC-IRMS zur Analyse von Zuckern in Honig ist ein innovativer Schritt in die Richtung, qualitativ hochwertige und authentische Produkte anzubieten. Damit stellt die EN 17958:2024 nicht nur ein wichtiges Werkzeug für Fachleute und Produzenten dar, sondern trägt auch zur gesetzlichen Einhaltung und zur Überwachung von Lebensmittelstandards bei, ohne jedoch den Prozess der Konformitätsbewertung zu behandeln, was den Fokus auf die analytische Methodik verstärkt.
표준 EN 17958:2024는 꿀에 포함된 단당류(과당 및 포도당), 이당류 및 삼당류의 탄소 안정 동위 원소 비율(δ13C 값)을 액체 크로마토그래피-동위 원소 비율 질량 분석법(LC-IRMS)을 사용하여 결정하는 방법을 규정하고 있습니다. 이 문서는 꿀의 진품 여부를 평가하기 위한 기준으로 매우 중요하며, 꿀의 13C/12C 비율을 통해 진품 꿀과 불순물(전분이 풍부한 식물이나 사탕수수, 사탕무에서 만든 시럽 등)에서의 차이를 확인할 수 있는 방법을 제공합니다. 이 표준의 강점은 액체 크로마토그래피와 동위 원소 비율 질량 분석기를 결합하여 고도로 정밀한 분석을 가능하게 한다는 점입니다. 이러한 기술적 접근은 꿀의 진품성을 검증하기 위해 필수적인 요소로 작용하며, 저작권 전문가들에 의해 인정된 가이드 값과의 비교를 통해 높은 신뢰도를 제공합니다. 이러한 비교 분석은 농산물의 무결성을 보장하고 소비자에게 품질 높은 제품을 제공하는 데 필수적인 역할을 합니다. 또한, 이 표준은 꿀 인증 및 품질 관리 과정에서 중요한 도구로 활용될 수 있으며, 꿀 산업의 신뢰도를 높이는 데 기여할 것입니다. 표준화된 방법론을 통해 생산자와 소비자 양측에서 더욱 투명하고 신뢰할 수 있는 시장 환경이 조성될 것으로 기대됩니다. EN 17958:2024는 꿀의 진품 인증을 위한 필수적이고 실용적인 가이드라인을 제공하며, 관련 분야의 전문가들이 활용할 수 있는 현실적인 기준을 설정하는 데 기여하고 있습니다.
EN 17958:2024 の標準は、はちみつ中の単糖（フルクトースおよびグルコース）、二糖、三糖の炭素同位体比（δ13C値）を液体クロマトグラフィーと同位体比質量分析法（LC-IRMS）を用いて測定するための方法を明確に規定しています。この文書の範囲は、はちみつの真偽を評価するために重要な役割を果たします。 この標準の大きな強みは、LC-IRMS技術を使用することにより、はちみつ中の糖類の詳細な同位体比を取得できる点にあります。13C/12C比は、正規のはちみつと不正な添加物（デンプンが豊富な植物やサトウキビ、糖ビートから作られたシロップ）の間で顕著な違いがあるため、はちみつの本物性を確実に評価するための信頼性の高いデータを提供します。このアプローチは、はちみつの品質管理や市場での信頼性を保持するために不可欠です。 加えて、専門家によって導出されたガイダンス値との比較を通じて、はちみつの真偽は容易に判定できます。これは、食品の真偽を保証する上での重要な要素であり、消費者保護や市場の健全性を確保するための基盤となります。 ただし、この文書には遵守評価プロセスは含まれていないため、実際の運用においては注意が必要です。全体として、EN 17958:2024は食の安全性と品質を向上させるための貴重な資源となる標準であり、はちみつの真偽を評価するための効果的な方法を提供します。
La norme SIST EN 17958:2025 fournit une méthode précise et fiable pour la détermination de la valeur δ13C des mono- (fructose et glucose), di- et trisaccharides présents dans le miel. Grâce à l'utilisation de la chromatographie liquide couplée à une spectrométrie de masse à rapport isotopique (LC-IRMS), cette norme assure une séparation efficace des composés et une mesure précise du rapport 13C/12C des sucres. Le champ d'application de cette norme est particulièrement pertinent dans le contexte actuel où l'authenticité des denrées alimentaires est devenue une préoccupation majeure. En effet, les rapports isotopiques obtenus grâce à cette méthode peuvent être comparés à des valeurs de référence convenues pour le miel authentique, fournies par des experts en la matière. Cela permet d'identifier clairement les miels authentiques par rapport aux miels adulterés, souvent issus de sirops fabriqués à partir de plantes riches en amidon ou de canne à sucre et de betterave à sucre, qui présentent des ratios 13C/12C distincts. Parmi les forces de cette norme, on peut souligner sa méthodologie rigoureuse qui garantit non seulement la précision des mesures, mais aussi la reproductibilité des résultats, ce qui est essentiel pour établir la crédibilité des analyses menées. En outre, l'intégration d'instruments de pointe tels que le LC-IRMS dans le processus de mesure renforce l'efficacité de cette norme dans la lutte contre la fraude alimentaire. Dans un contexte où la traçabilité et la transparence des produits alimentaires sont de plus en plus valorisées, la norme SIST EN 17958:2025 s'avère être un outil indispensable pour les producteurs de miel, les laboratoires d'analyse et les régulateurs. En assurant la conformité avec des normes rigoureuses, elle contribue à garantir la qualité des produits offerts aux consommateurs, renforçant ainsi la confiance dans le marché du miel.
The EN 17958:2024 standard presents a significant advancement in the field of food authenticity, particularly in determining the δ13C value of sugars present in honey through the application of liquid chromatography-isotope ratio mass spectrometry (LC-IRMS). The scope of this standard effectively delineates the methodology for accurately measuring the stable isotopes of carbon, specifically the 13C/12C ratios of mono- (fructose and glucose), di-, and trisaccharides in honey. One of the strengths of this standard is its comprehensive approach to authentication; it not only outlines the technical procedures for analysis but also emphasizes the importance of comparing the obtained isotopic ratios against established guidance values identified by subject matter experts. This comparison is vital for the detection of potential adulteration, as the δ13C values of sugars in genuine honey differ from those found in syrups made from starch sources or sugar cane and sugar beet. Additionally, the document underlines the reliability of the LC-IRMS technique, recognized for its precision and sensitivity. By providing a structured protocol for the determination of sugar δ13C values, the standard ensures that honey producers, regulators, and consumers can have confidence in the authenticity of honey products. Moreover, EN 17958:2024 is relevant in the context of increasing consumer demand for transparency and quality in food products. As the food industry faces growing scrutiny regarding adulteration practices, this standard provides a necessary framework for testing and validation, contributing to the integrity of the honey market. In conclusion, the EN 17958:2024 standard represents a critical tool in the pursuit of food authenticity, combining scientific rigor with practical application to support the assessment of honey purity and quality.
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