EN 15634-4:2023

Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

This document specifies a method for the detection of peanut (Arachis hypogaea) in chocolate.
Real-time PCR (Polymerase Chain Reaction) detection of peanut is based on an 86 bp (base pair) sequence from the Ara h 2 gene of peanut.

Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen Verfahren - Teil 4: Erdnuss (Arachis hypogaea) - Qualitativer Nachweis einer spezifischen DNA-Sequenz in Schokolade mittels Real-time PCR

Dieses Dokument legt ein Verfahren zum Nachweis von Erdnuss (Arachis hypogaea) in Schokolade fest.
Die Real time-PCR (Polymerase-Kettenreaktion) zum Nachweis von Erdnuss beruht auf einer 86 bp (Basenpaar) großen Sequenz des Ara h 2 Gens der Erdnuss.

Produits alimentaires - Détection des allergènes alimentaires par des méthodes d’analyse de biologie moléculaire - Partie 4 : Arachide (Arachis hypogaea) - Détection qualitative d’une séquence d’ADN spécifique dans du chocolat, par PCR en temps réel

Le présent document décrit une méthode de détection de l’arachide (Arachis hypogaea) dans du chocolat.
La détection de l’arachide par PCR (réaction de polymérisation en chaîne) en temps réel repose sur une séquence de 86 pb (paires de bases) provenant du gène Ara h 2 de l’arachide.

Živila - Določevanje alergenov v živilih z molekularno biološkimi metodami - 4. del: Arašidi (Arachis hypogaea) - Kvalitativno določanje specifičnega zaporedja DNK v čokoladi s PCR v realnem času

Ta metoda opisuje postopek za kvalitativno odkrivanje arašidov (Arachis hypogaea) v
v čokoladi z verižno reakcijo s polimerazo (PCR) v realnem času na podlagi gena za alergen iz arašida Ara h 2 [4, 5].

General Information

• Status: Published
• Publication Date: 21-Feb-2023
• ICS: 07.100.30 - Food microbiology
• ICS: 67.190 - Chocolate
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 12 - Food allergens
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 22-Feb-2023
• Due Date: 15-Aug-2022
• Completion Date: 22-Feb-2023
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: SIST EN 15634-4:2023 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

Relations

• Replaces: CEN/TS 15634-4:2016 - Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut (Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by real-time PCR
• Effective Date: 19-Jan-2023

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 15634-4:2023
01-maj-2023
Živila - Določevanje alergenov v živilih z molekularno biološkimi metodami - 4. del:
Arašidi (Arachis hypogaea) - Kvalitativno določanje specifičnega zaporedja DNK v
čokoladi s PCR v realnem času
Foodstuffs - Detection of food allergens by molecular biological methods - Part 4: Peanut
(Arachis hypogaea) - Qualitative detection of a specific DNA sequence in chocolate by
real-time PCR
Lebensmittel - Nachweis von Lebensmittelallergenen mit molekularbiologischen
Verfahren - Teil 4: Erdnuss (Arachis hypogaea) - Qualitativer Nachweis einer
spezifischen DNA-Sequenz in Schokolade mittels Real-time PCR
Produits alimentaires - Détection des allergènes alimentaires par des méthodes
d’analyse de biologie moléculaire - Partie 4 : Arachide (Arachis hypogaea) - Détection
qualitative d’une séquence d’ADN spécifique dans du chocolat, par PCR en temps réel
Ta slovenski standard je istoveten z: EN 15634-4:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.190 Čokolada Chocolate
SIST EN 15634-4:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 15634-4:2023

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SIST EN 15634-4:2023
EN 15634-4
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2023
EUROPÄISCHE NORM
ICS 07.100.30; 67.190 Supersedes CEN/TS 15634-4:2016
English Version
Foodstuffs - Detection of food allergens by molecular
biological methods - Part 4: Peanut (Arachis hypogaea) -
Qualitative detection of a specific DNA sequence in
chocolate by real-time PCR
Produits alimentaires - Détection des allergènes Lebensmittel - Nachweis von Lebensmittelallergenen
alimentaires par des méthodes d'analyse de biologie mit molekularbiologischen Verfahren - Teil 4:
moléculaire - Partie 4 : Arachide (Arachis hypogaea) - Erdnuss (Arachis hypogaea) - Qualitativer Nachweis
Détection qualitative d'une séquence d'ADN spécifique einer spezifischen DNA-Sequenz in Schokolade
dans du chocolat, par PCR en temps réel mittels Real-time PCR
This European Standard was approved by CEN on 16 January 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
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United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15634-4:2023 E
worldwide for CEN national Members.

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SIST EN 15634-4:2023
EN 15634-4:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents .
...