Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance

SIGNIFICANCE AND USE
5.1 This practice provides laboratory procedures for rating the relative bioresistance of metalworking fluid formulations, for determining the need for microbicide addition prior to or during fluid use in metalworking systems and for evaluating microbicide performance. General considerations for microbicide selection are provided in Practice E2169.  
5.2 The factors affecting challenge population numbers, taxonomic diversity, physiological state, inoculation frequency and biodeterioration effects in recirculating metalworking fluid systems are varied and only partially understood. Consequently, the results of tests completed in accordance with this practice should be used only to compare the relative performance of products or microbicide treatments included in a test series. Results should not be construed as predicting actual field performance.
SCOPE
1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D3946 and E686.  
1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change, physical property change or some combination thereof.  
1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant components.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Publication Date
31-Jul-2014
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Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2275 − 14
Standard Practice for
Evaluating Water-Miscible Metalworking Fluid Bioresistance
1
and Antimicrobial Pesticide Performance
This standard is issued under the fixed designation E2275; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope D888 Test Methods for Dissolved Oxygen in Water
D1067 Test Methods for Acidity or Alkalinity of Water
1.1 This practice addresses the evaluation of the relative
D1193 Specification for Reagent Water
inherent bioresistance of water-miscible metalworking fluids,
D3342 Test Method for Dispersion Stability of New (Un-
the bioresistance attributable to augmentation with antimicro-
used) Rolling Oil Dispersions in Water
bial pesticides or both. It replaces Methods D3946 and E686.
D3519 Test Method for Foam in Aqueous Media (Blender
1.2 In this practice relative bioresistance is determined by 3
Test) (Withdrawn 2013)
challenging metalworking fluids with a biological inoculum
D3601 Test Method for Foam In Aqueous Media (Bottle
that may either be characterized (comprised of one or more 3
Test) (Withdrawn 2013)
known biological cultures) or uncharacterized (comprised of
D4627 Test Method for Iron Chip Corrosion for Water–Mis-
biologically contaminated metalworking fluid or one or more
cible Metalworking Fluids
unidentified isolates from deteriorated metalworking fluid).
D5465 Practice for Determining Microbial Colony Counts
Challenged fluid bioresistance is defined in terms of resistance
from Waters Analyzed by Plating Methods
to biomass increase, viable cell recovery increase, chemical
E70 Test Method for pH of Aqueous Solutions With the
property change, physical property change or some combina-
Glass Electrode
tion thereof.
E1326 Guide for Evaluating Non-culture Microbiological
1.3 This practice is applicable to antimicrobial agents that Tests Used for Enumerating Bacteria
E2169 Practice for Selecting Antimicrobial Pesticides for
are incorporated into either the metalworking fluid concentrate
or end-use dilution. It is also applicable to metalworking fluids Use in Water-Miscible Metalworking Fluids
E2523 Terminology for Metalworking Fluids and Opera-
that are formulated using non-microbicidal, inherently biore-
sistant components. tions
E2563 Practice for Enumeration of Non-Tuberculosis Myco-
1.4 The values stated in SI units are to be regarded as
bacteria in Aqueous Metalworking Fluids by Plate Count
standard. No other units of measurement are included in this
Method
standard.
E2564 Practice for Enumeration of Mycobacteria in Metal-
1.5 This standard does not purport to address all of the
working Fluids by Direct Microscopic Counting (DMC)
safety concerns, if any, associated with its use. It is the
Method
responsibility of the user of this standard to establish appro-
E2657 Test Method for Determination of Endotoxin Con-
priate safety and health practices and determine the applica-
centrations in Water-Miscible Metalworking Fluids
bility of regulatory limitations prior to use.
E2694 Test Method for Measurement ofAdenosine Triphos-
phate in Water-Miscible Metalworking Fluids
2. Referenced Documents
E2756 Terminology Relating to Antimicrobial and Antiviral
2
2.1 ASTM Standards:
Agents
D1129 Terminology Relating to Water
E2889 Practice for Control of Respiratory Hazards in the
Metal Removal Fluid Environment
1
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides,
2.2 Other Standards:
Antimicrobials, and Alternative Control Agents and is the direct responsibility of
AOAC 960.9 Germicidal and Detergent Sanitizing Action
Subcommittee E35.15 on Antimicrobial Agents.
4
Disinfectants
Current edition approved Aug. 1, 2014. Published September 2014. Originally
approved in 2003. Last previous edition approved in 2013 as E2275 – 13. DOI:
10.1520/E2275-14.
2 3
For referenced ASTM standards, visit the ASTM website, www.astm.org, or The last approved version of this historical standard is referenced on
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM www.astm.org.
4
Standards volume information, refer to the standard’s Document Summary page on AOAC International Methods of Analysis, AOAC International, Gaithersburg,
the ASTM website. MD.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2275 − 14
9215A.6a Heterotrophic Plate Count Media, Plate Count 3.3 Abbreviations:
5
Agar 3.3.1 a.i.—active ingredient
5
9216 Direct Total Microbial Count 3.3.2 a.s.—as supplied
6
Microbiological Test <71> 3.3.3 ATCC—a
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2275 − 13 E2275 − 14
Standard Practice for
Evaluating Water-Miscible Metalworking Fluid Bioresistance
1
and Antimicrobial Pesticide Performance
This standard is issued under the fixed designation E2275; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice addresses the evaluation of the relative inherent bioresistance of water-miscible metalworking fluids, the
bioresistance attributable to augmentation with antimicrobial pesticides or both. It replaces Methods D3946 and E686.
1.2 In this practice relative bioresistance is determined by challenging metalworking fluids with a biological inoculum that may
either be characterized (comprised of one or more known biological cultures) or uncharacterized (comprised of biologically
contaminated metalworking fluid or one or more unidentified isolates from deteriorated metalworking fluid). Challenged fluid
bioresistance is defined in terms of resistance to biomass increase, viable cell recovery increase, chemical property change,
physical property change or some combination thereof.
1.3 This practice is applicable to antimicrobial agents that are incorporated into either the metalworking fluid concentrate or
end-use dilution. It is also applicable to metalworking fluids that are formulated using non-microbicidal, inherently bioresistant
components.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information
only.standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D888 Test Methods for Dissolved Oxygen in Water
D1067 Test Methods for Acidity or Alkalinity of Water
D1193 Specification for Reagent Water
D3342 Test Method for Dispersion Stability of New (Unused) Rolling Oil Dispersions in Water
3
D3519 Test Method for Foam in Aqueous Media (Blender Test) (Withdrawn 2013)
3
D3601 Test Method for Foam In Aqueous Media (Bottle Test) (Withdrawn 2013)
D4627 Test Method for Iron Chip Corrosion for Water–Miscible Metalworking Fluids
D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
E70 Test Method for pH of Aqueous Solutions With the Glass Electrode
E1326 Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids
E2523 Terminology for Metalworking Fluids and Operations
E2563 Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
E2564 Practice for Enumeration of Mycobacteria in Metalworking Fluids by Direct Microscopic Counting (DMC) Method
E2657 Test Method for Determination of Endotoxin Concentrations in Water-Miscible Metalworking Fluids
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2013Aug. 1, 2014. Published November 2013September 2014. Originally approved in 2003. Last previous edition approved in 20082013
as E2275 – 03E2275 – 13.(2008). DOI: 10.1520/E2275-13.10.1520/E2275-14.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
The last approved version of this historical standard is referenced on www.astm.org.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2275 − 14
E2694 Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
E2889 Practice for Control of Respiratory Hazards in the Metal
...

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