ASTM E2783-11
(Test Method)Standard Test Method for Assessment of Antimicrobial Activity for Water Miscible Compounds Using a Time-Kill Procedure
Standard Test Method for Assessment of Antimicrobial Activity for Water Miscible Compounds Using a Time-Kill Procedure
SIGNIFICANCE AND USE
This procedure may be used to assess the in vitro reduction of a microbial population of test organisms after exposure to a test material.
SCOPE
1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling time when tested against antimicrobial test materials in vitro. The organisms used are standardized as to growth requirements and inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous systems.
1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL.
1.3 Knowledge of microbiological techniques is required for this procedure.
1.4 Aseptic technique should be practiced at all times.
1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units follow in parentheses.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation: E2783 − 11
StandardTest Method for
Assessment of Antimicrobial Activity for Water Miscible
1
Compounds Using a Time-Kill Procedure
This standard is issued under the fixed designation E2783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3. Terminology
1.1 This test method measures the changes of a population 3.1 Definitions:
of aerobic and anaerobic microorganisms within a specific
3.1.1 antimicrobial, n—describes an agent that kills or
sampling time when tested against antimicrobial test materials
inactivates microorganisms or suppresses their growth or
in vitro. The organisms used are standardized as to growth
reproduction.
requirements and inoculum preparation and must grow under
3.1.2 drug, n—articles intended for use in the diagnosis,
the conditions of the test. The primary purpose of this test
cure, mitigation, treatment, or prevention of disease in man or
method is to provide a set of standardized conditions and test
other animals. Drugs are intended to affect the structure or any
organisms to facilitate comparative assessments of antimicro-
function of the body of man or other animals.
bial materials miscible in aqueous systems.
3.1.3 initial microbial population, n—bacterial count (CFU/
1.2 Thistestmethodallowstheoptionofusingatestsample
mL) in the final volume of test material.Also known as initial
size of 10 mL or 100 mL.
bacterial population, numbers control or control.
1.3 Knowledge of microbiological techniques is required
3.1.4 inoculum, n—the viable microorganisms used to con-
for this procedure.
taminate a sample, device or surface, often expressed as to
1.4 Aseptic technique should be practiced at all times.
number and type.
1.5 Inthistestmethod,SIunitsareusedforallapplications,
3.1.5 microbiocide, n—a physical or chemical agent that
except for distance in which case inches are used and SI units
kills microorganisms.
follow in parentheses.
3.1.6 neutralization, n—the process for inactivating or
1.6 This standard does not purport to address all of the
quenching the activity of a microbiocide. Often achieved
safety concerns, if any, associated with its use. It is the
through chemical or physical means (for example, filtration or
responsibility of the user of this standard to establish appro-
dilution).
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
3.1.7 room temperature, n—temperature in the range of 20
to 30°C (68 to 85°F).
2. Referenced Documents
3.1.8 test material, n—a formulation which incorporates
2
2.1 ASTM Standards:
antimicrobial ingredient(s). Also known as test formulation.
E691Practice for Conducting an Interlaboratory Study to
3.1.9 total test volume, n—the volume of test material plus
Determine the Precision of a Test Method
the volume of inoculum suspension.
E1054Test Methods for Evaluation of Inactivators of Anti-
microbial Agents
4. Summary of Test Method
4.1 A dilution/aliquot of the test material is brought into
1
This test method is under the jurisdiction of ASTM Committee E35 on contactwithaknownpopulationoftestorganismsforspecified
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
periods of time, at a specified temperature. The activity of the
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
test material is quenched at specified sampling intervals
Current edition approved Oct. 1, 2011. Published October 2011. Originally
(example 15, 30, and 60 s, or any range covering several
approved in 2010. Last previous edition approved in 2010 as E2783–10. DOI:
10.1520/E2783–11.
minutes or hours) with an appropriate neutralizing technique.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
The test material is neutralized at the sampling time and the
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
surviving microorganisms enumerated. The percent and log
Standards volume information, refer to the standard’s Document Summary page on 10
the ASTM website. reduction, from an initial microbial population is calculated.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
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E2783 − 11
5. Significance and Use 7.4 Dilution Fluid or Diluent—SterileButterfield’sbuffered
3
phosphate diluent or other suitable diluent with appropriately
5.1 This procedure may be used to assess the in vitro
validated neutralizers. Perform Test Method E1054 to deter-
reduction of a microbial population of test organisms af
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2783–10 Designation:E2783–11
Standard Test Method for
Assessment ofAntimicrobialActivity for Water Miscible
1
Compounds Using a Time-Kill Procedure
This standard is issued under the fixed designation E2783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling
time when tested against antimicrobial test materials in vitro. The organisms used are standardized as to growth requirements and
inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set
of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous
systems.
1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL.
1.3 Knowledge of microbiological techniques is required for this procedure.
1.4 Aseptic technique should be practiced at all times.
1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units
follow in parentheses.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2315Guide forAssessment ofAntimicrobialActivity Using aTime-Kill ProcedureTest Methods for Evaluation of Inactivators
of Antimicrobial Agents
3. Terminology
3.1 Definitions:
3.1.1 antimicrobial, n—describes an agent that kills or inactivates microorganisms or suppresses their growth or reproduction.
3.1.2 drug, n—articles intended for use in the diagnosis, cure, mitigation, treatment, or prevention of disease in man or other
animals. Drugs are intended to affect the structure or any function of the body of man or other animals.
3.1.3 initial microbial population, n—bacterial count (CFU/mL) in the final volume of test material. Also known as initial
bacterial population, numbers control or control.
3.1.4 inoculum, n—the viable microorganisms used to contaminate a sample, device or surface, often expressed as to number
and type.
3.1.5 microbiocide, n—a physical or chemical agent that kills microorganisms.
3.1.6 neutralization, n—the process for inactivating or quenching the activity of a microbiocide. Often achieved through
chemical or physical means (for example, filtration or dilution).
3.1.7 room temperature, n—temperature in the range of 20 to 30°C (68 to 85°F).
3.1.8 test material, n—a formulation which incorporates antimicrobial ingredient(s). Also known as test formulation.
3.1.9 total test volume, n—the volume of test material plus the volume of inoculum suspension.
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2010. Published November 2010. DOI: 10.1520/E2783–10.
Current edition approved Oct. 1, 2011. Published October 2011. Originally approved in 2010. Last previous edition approved in 2010 as E2783 – 10. DOI:
10.1520/E2783–11.
2
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
E2783–11
4. Summary of Test Method
4.1 Adilution/aliquotofthetestmaterialisbroughtintocontactwithaknownpopulationoftestorganismsforspecifiedperiods
of time, at a specified temperature. The activity of the test material is quenched at specified sampling intervals (example 15, 30,
and 60 s, or any range covering several minutes or hours) with an appropriat
...
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