ASTM E2458-10
(Practice)Standard Practices for Bulk Sample Collection and Swab Sample Collection of Visible Powders Suspected of Being Biological Agents from Nonporous Surfaces
Standard Practices for Bulk Sample Collection and Swab Sample Collection of Visible Powders Suspected of Being Biological Agents from Nonporous Surfaces
SIGNIFICANCE AND USE
These practices should be used only to collect visible samples that are suspected biothreat agents and have been field screened as defined by the FBI-DHS-HHS/CDC Coordinated Document for explosive hazard, radiological hazard, and other acute chemical hazards.
SCOPE
1.1 These practices address collection of visible powders that are suspected biothreat agents from solid nonporous surfaces using a bulk collection method, using a dry swab and laminated card, followed by a swab sampling method using a sterile moistened swab. Bulk powder samples are collected and packaged in a manner that permits the maximum amount of the sample to be safely transported to a reference laboratory within the Centers for Disease Control and Prevention (CDC) national Laboratory Response Network (LRN) for confirmatory identification and safe storage. If the source of the powder is a letter or small package, that item is also packaged in a manner that permits it to be safely transported to an LRN reference laboratory. A sterile moistened swab may be used to collect residual powder and may be used to conduct on-site biological assessments for the purpose of testing for biothreat agents.
1.2 These practices are performed in coordination with the Federal Bureau of Investigation (FBI) as part of a risk assessment including hazard assessment and threat evaluation as recommended and clarified in Guide . The decision to implement these practices and collect a public safety sample will be made by members of the response community of the jurisdiction assuming responsibility through coordination with the FBI and the receiving LRN reference laboratory.
1.3 Sample Collection Method A covers the bulk collection and packaging of suspicious visible powders that are suspected biothreat agents from solid nonporous surfaces. All samples suspected to be biothreat agents on nonporous surfaces should be collected according to Sample Collection Method A and sent to a LRN reference laboratory for confirmatory testing.
1.4 Sample Collection Method B covers swab sampling of residual suspicious powders that are suspected biothreat agents from solid nonporous surfaces. Swab samples can be used for on-site biological assessment; however results from on-site biological assessments are not definitive; confirmatory testing by the LRN reference laboratory is necessary to make public health decisions.
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Designation: E2458 − 10
Standard Practices for
Bulk Sample Collection and Swab Sample Collection of
Visible Powders Suspected of Being Biothreat Agents from
1
Nonporous Surfaces
This standard is issued under the fixed designation E2458; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope from solid nonporous surfaces. Swab samples can be used for
on-site biological assessment; however results from on-site
1.1 These practices address collection of visible powders
biological assessments are not definitive; confirmatory testing
that are suspected biothreat agents from solid nonporous
by the LRN reference laboratory is necessary to make public
surfaces using a bulk collection method, using a dry swab and
health decisions.
laminated card, followed by a swab sampling method using a
sterilemoistenedswab.Bulkpowdersamplesarecollectedand 1.5 These practices incorporate reference guidance for
packagedinamannerthatpermitsthemaximumamountofthe packaging and transport of suspicious visible powders to
sample to be safely transported to a reference laboratory within comply with all appropriate federal regulations regarding
theCentersforDiseaseControlandPrevention(CDC)national
biosafety and biosecurity.
2
Laboratory Response Network (LRN) for confirmatory iden-
1.6 These practices should only be used to collect visible
tificationandsafestorage.Ifthesourceofthepowderisaletter
samples that are suspected biothreat agents and have been field
or small package, that item is also packaged in a manner that
screened according to reference guidance for explosive hazard,
permits it to be safely transported to an LRN reference
radiological hazard, and other acute chemical hazards.
laboratory. A sterile moistened swab may be used to collect
1.7 The values stated in SI units are to be regarded as
residual powder and may be used to conduct on-site biological
standard. The values given in parentheses are for information
assessments for the purpose of testing for biothreat agents.
only.
1.2 These practices are performed in coordination with the
1.8 This standard does not purport to address all of the
Federal Bureau of Investigation (FBI) as part of a risk
safety concerns associated with its use. It is the responsibility
assessment including hazard assessment and threat evaluation
of the user of this standard to establish appropriate safety and
as recommended and clarified in Guide E2770.The decision to
health practices and determine the applicability of regulatory
implement these practices and collect a public safety sample
limitations prior to use.
will be made by members of the response community of the
jurisdiction assuming responsibility through coordination with
2. Referenced Documents
the FBI and the receiving LRN reference laboratory.
3
2.1 ASTM Standards:
1.3 Sample Collection Method A covers the bulk collection
E2770 GuideforOperationalGuidelinesforInitialResponse
and packaging of suspicious visible powders that are suspected
to a Suspected Biothreat Agent
biothreat agents from solid nonporous surfaces. All samples
F2412 Test Methods for Foot Protection
suspected to be biothreat agents on nonporous surfaces should
F2413 Specification for Performance Requirements for Pro-
becollectedaccordingtoSampleCollectionMethodAandsent
tective (Safety) Toe Cap Footwear
to a LRN reference laboratory for confirmatory testing.
4
2.2 Federal Government Regulations:
1.4 Sample Collection Method B covers swab sampling of
18 USC 175 Prohibitions with respect to biological weapons
residual suspicious powders that are suspected biothreat agents
DOT 49 CFR, Parts 171-180 Hazardous Materials Regula-
tions
1
These practices are under the jurisdiction of ASTM Committee E54 on
Homeland Security Applications and are the direct responsibility of Subcommittee
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E54.01 on CBRNE Sensors and Detectors. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved Oct. 1, 2010. Published November 2010. Originally contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
approved in 2006. Last previous edition approved in 2006 as E2458 – 06. DOI: Standards volume information, refer to the standard’s Document Summary page on
10.1520/E2458-10. the ASTM website.
4
2
The CDC Laboratory Response Network is the network responsible for AvailablefromU.S.GovernmentPrintingOfficeSuperintendentofDocuments,
handling clinical specimens and environmental samples containing suspected 732N.CapitolSt.,NW,MailStop:SDE,Washington,DC20401,andalsoavailable
biothreat agents. online from Occupational Safety and Health Administrati
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2458–06 Designation:E2458–10
Standard Practices for
Bulk Sample Collection and Swab Sample Collection of
Visible Powders Suspected of Being BiologicalBiothreat
1
Agents from Nonporous Surfaces
This standard is issued under the fixed designation E2458; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 These practices address collection of visible powders that are suspected biologicalbiothreat agents from solid nonporous
surfaces using a bulk collection method, using a dry swab and laminated card, followed by a swab sampling method using a sterile
moistened swab. Bulk powder samples are collected and packaged in a manner that permits the maximum amount of the sample
tobesafelytransportedtoanapprovedareferencelaboratorywithintheCentersforDiseaseControlandPrevention(CDC)national
2
Laboratory Response Network (LRN) for safe storage, confirmatory analysis, and forensic testing. If the source of the powder is
a letter or small package, the source is also packaged in a manner that permits it to be safely transported to the laboratory in the
CDC Laboratory Response Network. Swab samples taken using a sterile moistened swab are used to collect residual powder and
may be used for on-site screening and presumptive testing (biological screening).
1.2These practices are performed after a risk assessment is conducted and a visible powder is deemed a credible biological
threat.
1.3Sample Collection Method A covers the bulk collection and packaging of suspicious visible powders that are suspected
biological agents from solid nonporous surfaces.
1.4Sample Collection Method B covers swab sampling of residual suspicious powders that are suspected biological agents from
solid nonporous surfaces. Swab samples can be used for on-site screening and presumptive testing (biological screening). These
presumptive tests are either confirmed or not confirmed by additional testing at the laboratory in the CDC Laboratory Response
Network using samples collected in Sample Collection Method A. for confirmatory identification and safe storage. If the source
of the powder is a letter or small package, that item is also packaged in a manner that permits it to be safely transported to an LRN
reference laboratory. A sterile moistened swab may be used to collect residual powder and may be used to conduct on-site
biological assessments for the purpose of testing for biothreat agents.
1.2 These practices are performed in coordination with the Federal Bureau of Investigation (FBI) as part of a risk assessment
including hazard assessment and threat evaluation as recommended and clarified in Guide E2770.The decision to implement these
practices and collect a public safety sample will be made by members of the response community of the jurisdiction assuming
responsibility through coordination with the FBI and the receiving LRN reference laboratory.
1.3 Sample Collection Method A covers the bulk collection and packaging of suspicious visible powders that are suspected
biothreat agents from solid nonporous surfaces. All samples suspected to be biothreat agents on nonporous surfaces should be
collected according to Sample Collection Method A and sent to a LRN reference laboratory for confirmatory testing.
1.4 Sample Collection Method B covers swab sampling of residual suspicious powders that are suspected biothreat agents from
solid nonporous surfaces. Swab samples can be used for on-site biological assessment; however results from on-site biological
assessments are not definitive; confirmatory testing by the LRN reference laboratory is necessary to make public health decisions.
1.5 These practices incorporate reference guidance for packaging and transport of suspicious visible powders to comply with
all appropriate federal regulations regarding biosafety and biosecurity.
1.6These practices should only be used to collect visible samples that are suspected biological hazards and have been screened
according to reference guidance for explosive hazard, radiological hazard, and other acute chemical hazards.
1.7The bulk sample collection practice and the swab sampling practice are recommended for collecting amassed or dispersed
powder samples from all nonporous surfaces on which the suspicious powder sample is clearly visible.
1
These practices are under the jurisd
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