EN 17424:2020

Foodstuffs - Determination of aflatoxins in spices other than paprika by IAC clean-up and HPLC-FLD with post-column derivatization

This document describes a procedure for the determination of aflatoxins B1, B2, G1 and G2 and total aflatoxins (sum of B1, B2, G1 and G2) in spices for which EU maximum levels are established, other than paprika, by high performance liquid chromatography (HPLC) with post-column derivatization (PCD) and fluorescence detection (FLD) after immunoaffinity column (IAC) clean-up.
The method is applicable to the spices capsicum (excluding paprika), pepper, nutmeg, ginger, turmeric and mixtures thereof.
The method has been validated for aflatoxins B1, B2, G1 and G2 and total aflatoxins in a range of test samples that comprised: ginger, pepper, nutmeg, chilli, turmeric as individual spices and mixed pepper + chilli + nutmeg (90 + 5 + 5, m + m + m), mixed spice+ginger (6 + 4, m + m) mixed spice, mixed turmeric+ginger (2 + 8, m + m).
The validation was carried out over the following concentration ranges: aflatoxin B1 = 1 µg/kg to 16 µg/kg and total aflatoxins = 2,46 µg/kg to 36,1 µg/kg.

Lebensmittel - Bestimmung von Aflatoxinen in Gewürzen außer Paprika mit IAC Reinigung und HPLC-FLD mit Nachsäulenderivatisierung

Dieses Dokument beschreibt ein Verfahren zur Bestimmung der Aflatoxine B1, B2, G1 und G2 und des Gesamtgehalts an Aflatoxinen (Summe von B1, B2, G1 und G2) in Gewürzen außer Paprika, für die EU Höchstgehalte festgelegt wurden, mittels Hochleistungsflüssigchromatographie (en: High Performance Liquid Chromatography, HPLC) mit Nachsäulenderivatisierung (en: post column derivatization, PCD) und Fluoreszenz¬detektion (FLD) nach Reinigung an der Immunoaffinitätssäule (en: immunoaffinity column, IAC).
Das Verfahren ist anwendbar auf die Gewürze Capsicum (außer Paprika), Pfeffer, Muskatnuss, Ingwer, Kurkuma und Mischungen daraus.
Dieses Verfahren wurde für die Aflatoxine B1, B2, G1 und G2 und die Gesamtaflatoxine in einer Reihe von Untersuchungsproben validiert. Diese umfassten: Ingwer, Pfeffer, Muskatnuss, Chili, Kurkuma als einzelne Gewürze und gemischten Pfeffer + Chili + Muskatnuss (90 + 5+ 5, m + m + m), gemischte Gewürze + Ingwer (6 + 4, m + m) gemischtes Gewürz, gemischtes Kurkuma + Ingwer (2 + 8, m + m).
Die Validierung wurde für die folgenden Konzentrationsbereiche durchgeführt: Aflatoxin B1 = 1 µg/kg bis 16 µg/kg und Gesamtaflatoxine = 2,46 µg/kg bis 36,1 µg/kg.

Produits alimentaires - Dosage des aflatoxines dans les épices autres que le paprika par purification sur colonne d’immunoaffinité et CLHP-FLD avec dérivation post-colonne

Le présent document décrit un mode opératoire pour le dosage des aflatoxines B1, B2, G1 et G2, ainsi que des aflatoxines totales (somme des aflatoxines B1, B2, G1 et G2) dans les épices pour lesquelles des teneurs maximales européennes sont établies, autres que le paprika, par chromatographie liquide à haute performance (CLHP) avec dérivation post-colonne (PCD) et détection par fluorescence (FLD) après purification sur colonne d’immunoaffinité (CIA).
La méthode est applicable au piment (à l’exclusion du paprika), au poivre, à la muscade, au gingembre, au curcuma, ainsi qu’à leurs mélanges.
La méthode a été validée pour les aflatoxines B1, B2, G1 et G2, ainsi que pour les aflatoxines totales dans un éventail d’échantillons d’essai comprenant : du gingembre, du poivre, de la muscade, du piment, du curcuma seuls, et un mélange poivre + piment + muscade (90 + 5 + 5, m + m + m), un mélange d’épices + gingembre (6 + 4, m + m), un mélange d’épices, un mélange curcuma + gingembre (2 + 8, m + m).
La validation a été effectuée dans les gammes de concentration suivantes : aflatoxine B1 = 1 µg/kg à 16 µg/kg et aflatoxines totales = 2,46 µg/kg à 36,1 µg/kg.

Živila - Določevanje aflatoksina v začimbah, razen v papriki, z IAC-čiščenjem in HPLC-FLD s postkolonsko derivatizacijo

General Information

• Status: Published
• Publication Date: 10-Nov-2020
• Withdrawal Date: 30-May-2021
• ICS: 67.050 - General methods of tests and analysis for food products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 5 - Biotoxins
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 11-Nov-2020
• Due Date: 29-Jan-2020
• Completion Date: 11-Nov-2020
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/520 - Mandate for standardisation addressed to CEN for methods of analysis for mycotoxins in food
• Ref Project: SIST EN 17424:2021 - Foodstuffs - Determination of aflatoxins in spices other than paprika by IAC clean-up and HPLC-FLD with post-column derivatization

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 17424:2021
01-januar-2021
Živila - Določevanje aflatoksina v začimbah, razen v papriki, z IAC-čiščenjem in
HPLC-FLD s postkolonsko derivatizacijo
Foodstuffs - Determination of aflatoxins in spices other than paprika by IAC clean-up and
HPLC-FLD with post-column derivatization
Lebensmittel - Bestimmung von Aflatoxinen in Gewürzen außer Paprika mit IAC
Reinigung und HPLC-FLD mit Nachsäulenderivatisierung
Produits alimentaires - Détermination des aflatoxines dans les épices (pour lesquelles un
niveau maximal a été établi par l'UE) autres que le paprika
Ta slovenski standard je istoveten z: EN 17424:2020
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.220.10 Začimbe Spices and condiments
SIST EN 17424:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 17424:2021

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SIST EN 17424:2021


EN 17424
EUROPEAN STANDARD

NORME EUROPÉENNE

November 2020
EUROPÄISCHE NORM
ICS 67.050
English Version

Foodstuffs - Determination of aflatoxins in spices other
than paprika by IAC clean-up and HPLC-FLD with post-
column derivatization
Produits alimentaires - Dosage des aflatoxines dans les Lebensmittel - Bestimmung von Aflatoxinen in
épices autres que le paprika par purification sur Gewürzen außer Paprika mit IAC Reinigung und HPLC-
colonne d'immunoaffinité et CLHP-FLD avec dérivation FLD mit Nachsäulenderivatisierung
post-colonne
This European Standard was approved by CEN on 14 September 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17424:2020 E
worldwide for CEN national Members.

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SIST EN 17424:2021
EN 17424:2020 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 6
6 Apparatus and equipment . 10
7 Procedure . 11
7.1 Extraction of aflatoxins from the sample . 11
7.2 Determination of recovery .
...

SLOVENSKI STANDARD
oSIST prEN 17424:2019
01-oktober-2019
Živila - Določevanje aflatoksina v začimbah, razen v papriki, z IAC-čiščenjem in
HPLC-FLD s postkolonsko derivatizacijo
Foodstuffs - Determination of aflatoxins in spices other than paprika by IAC clean-up and
HPLC-FLD with post-column derivatization
Lebensmittel - Bestimmung von Aflatoxinen in Gewürzen außer Paprika mit IAC
Reinigung und HPLC-FLD mit Nachsäulenderivatisierung
Produits alimentaires - Détermination des aflatoxines dans les épices (pour lesquelles un
niveau maximal a été établi par l'UE) autres que le paprika
Ta slovenski standard je istoveten z: prEN 17424
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.220.10 Začimbe Spices and condiments
oSIST prEN 17424:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17424:2019

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oSIST prEN 17424:2019


DRAFT
EUROPEAN STANDARD
prEN 17424
NORME EUROPÉENNE

EUROPÄISCHE NORM

August 2019
ICS 67.050
English Version

Foodstuffs - Determination of aflatoxins in spices other
than paprika by IAC clean-up and HPLC-FLD with post-
column derivatization
Produits alimentaires - Détermination des aflatoxines Lebensmittel - Bestimmung von Aflatoxinen in
dans les épices (pour lesquelles un niveau maximal a Gewürzen außer Paprika mit IAC Reinigung und HPLC-
été établi par l'UE) autres que le paprika FLD mit Nachsäulenderivatisierung
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 275.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17424:2019 E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------
oSIST prEN 17424:2019
prEN 00275281:2019 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus and equipment . 9
7 Procedure .
...